Related Manuals for Menicon ShellPa Pro
Summary of Contents for Menicon ShellPa Pro
- Page 1 SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(1/32) Instruction Manual For ShellPa Pro (Model type : STMP) Lifescience Dept. Menicon Co.,Ltd.
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Page 2: Table Of Contents
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(2/32) ＜Table of contents＞ 1. Preparing the equipment -------------------------------P4 1.1. Checking the supplied items -------------------------------P4 1.2. Identifying parts -------------------------------P5 1.3. Setting and connecting of each items -------------------------------P6 1.4. Switch on and check initial position (original point). - Page 3 SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(3/32) 7. Parameter screen -------------------------------P25 7.1. "Calender + Time" set -------------------------------P25 7.2. "Hour Meter" -------------------------------P25 7.3. "Set Position" and "Pin Gap Adjust" -------------------------------P25 8. "Maintenance" and "Overhaul" alarm -------------------------------P27 <Maintenance: When operating time reaches 2,000 hours.>...
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Page 4: Preparing The Equipment
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(4/32) 1. Preparing the equipment 1.1. Checking the supplied items First, please check, there are below units and parts. Operation unit ×1unit Control unit ×1 unit Stretch chamber holder ×1holder Cover plate ×5plates... -
Page 5: Identifying Parts
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(5/32) 1.2. Identifying parts Stretch and relax point (bar) <Operation unit> Rigid point (bar) Access cover Connecting cable 凸 Fuse Vent hole Connecting cable 凹 <Control unit> Main switch... -
Page 6: Setting And Connecting Of Each Items
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(6/32) 1.3. Setting and connecting of each item Operation unit Control unit Figure1.Connecting and setting ShellPa Pro Beforehand, cleaning up closely, inside and outside of “operation unit” and “stretch chamber holder”, using ethanol. -
Page 7: Switch On And Check Initial Position (Original Point)
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(7/32) 1.4. Switch on and check initial position (original point). Below procedure, please check and conduct. Beforehand, please check nothing of an obstacle in chamber holder setting area on “Operation unit”.- -- [1] Please turn on a “main switch”... -
Page 8: Interface Layout And Summary Of Each Interface
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(8/32) 1.5. Interface layout and Summary of each interface <Interface layout> Operating Pattern A Main menu Operating Pattern A Setting Data Output ★ Stretch-Stop system Monitoring ★Same layout for PatternB1 Operating Pattern B1 ★Same layout for PatternB2... - Page 9 SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(9/32) <Summary of each interface> (Main menu): Select of stretch and relax pattern, and switching “Stretch-Stop system” mode on and off. Other, enter to “Stretch-Stop system setting” interface or “parameter setting” interface.
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Page 10: Preparing For Stretch Culture ( Seeding On Stretch Chamber)
Before stretch cell culture start, you need to prepare for cell seeding on and cell attaching to “Stretch chamber”. Photo: Cell seeding on and cell attaching to “Stretch chamber” in Menicon Co., Ltd. (Cell: L929, Chamber: SC4Dea, Check time: 24h. after seeding). -
Page 11: Basic Function And Operation
3. Basic function and operation 3.1. Selecting stretch pattern On “ShellPa Pro”, you can practice operation of some stretch and relax patterns. Select of each stretch and relax pattern is done in “Main menu” screen. (Please check area inside the red line in “Main menu” screen below.) Please check below explanation of each pattern. -
Page 12: Pattern B2 (Int.-Sin.): Sine Wave + Standstill
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(12/32) <Pattern B2 (Int.-Sin.): Sine wave + Standstill> On this pattern, you can set standstill time in addition to “Pattern B1”. If retention time set on zero, movement will be the same as “PattenB1”. Please refer to wave on “Operating Pattern”... -
Page 13: Setting Stretch And Relax Parameter
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(13/32) 3.2. Setting stretch and relax parameter You can set parameter on “Operating Pattern setting” screen. Each “Operating Pattern setting” screen is not same on each pattern. But standard parameters is two parameters below. - Page 14 SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(14/32) Key pad Other patterns, almost the same as “Pattern A”, but parameter contents of each Pattern is different. Please refer to below tables. Table2. Each parameter on “Pattern B1”...
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Page 15: Operating To Start And Stop
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(15/32) (First time) Relax movement time to non- 0.25 --- 30.00sec. stretch initial position (First time) Standstill time on relax (non- 0.0 sec. --- 86400.0sec.(24h.) stretch initial position) (Second time) Stretch movement time to 0.25 --- 30.00sec. -
Page 16: Stop Operation
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(16/32) (Attention) On stretch and relax movement( displayed “NO SET Position”), you cannot change from “Operating Pattern” screen to “ Main menu” screen (This is interlock). <Stop operation> Push “STOP” button on “Operating Pattern” screen. -
Page 17: Cycle Stop System" Mode
“Operation unit”, there is a possibility that the origin position may change. 4. “Cycle Stop system” mode “Cycle Stop system” mode makes “ShellPa Pro” stop, automatically, on setting counts of Stretch and relax cycle. 4.1. "Cycle Stop system" mode Setting On “Main menu”... -
Page 18: Cycle Stop System" Mode Operation
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(18/32) 4.2. "Cycle Stop system" mode operation <"Cycle Stop system" mode “START” and “STOP” operation> You can set “Cycle Stop system” mode ON and OFF on “Operating Pattern” screen. -
Page 19: Stretch-Stop System" Mode
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(19/32) Please set “Cycle Stop” off (touch “Cycle Stop”). If you operate “Cycle stop” mode again, please carry out ★( referring to “4.1. “Cycle Stop system” mode Setting” on the previous page). -
Page 20: Stretch-Stop System" Mode Monitoring
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(20/32) Number of Sets Number of repeated “Stretch Time + Stop Time” 1 --- 100sets conducted on order below, Stretch time Stop time First: Stretch Time 1 set Second: Stop Time First and Second repeat on set “Number of... -
Page 21: Stretch-Stop System" Mode "Start" And "Stop" Operation
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(21/32) <"Stretch-Stop system" mode “START” and ”STOP” operation> “START” and ”STOP” operation is identical with a standard “START” and ”STOP” operation. Please refer to “3.3. Operating to start and stop”. -
Page 22: Data Output To Usb, And Viewing Images On A Computer (Data Display Screen)
Page(22/32) 6. Data output to USB, and viewing images on a computer (Data Display screen) “ShellPa Pro” can store data of stretch and relax movement in USB, and on PC, you can check. 6.1. Set SD card If SD card and parameters of setting SD card has been set, Bar graph and volume number of setting SD card is displayed on “Main menu”... -
Page 23: Store Data Of Stretch And Relax Movement In Usb
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(23/32) When setting… 6.2. Store data of stretch and relax movement in USB You must conduct, First, Store Data in SD card. Second, after it, change over from SD card to USB. - Page 24 SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(24/32) After it, automatically, the display change to “USB memory”. Select “SD card → USB memory” on “USB memory” screen, the display change from “USB memory” to “SD card → USB memory” screen.
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Page 25: Parameter Screen
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(25/32) 7. “Parameters” screen 7.1. ”Calender + Time” You can change and set, calender and time. On “Parameters” screen, please touch Number which you want to change, after, “Key pad” will be displayed on the screen. - Page 26 SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(26/32) (Stretch and relax movement) --- Push “Stop”: “Pin Gap Adjust” → “Set Position” (Stationary) (Attention: Machine is always located on “Set Position”, in stop state.) ========== Lifescience Dept. Menicon Co.,Ltd.
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Page 27: Maintenance" And "Overhaul" Alarm
Instruction Manual For ShellPa Pro (Model type : STMP) Page(27/32) 8. “Maintenance” and “Overhaul” alarm “ShellPa Pro” will inform you that operating time reaches a certain time. Please check below. (Attention: These alarms are just information. Therefore, on these alarms, equipment can operate, ordinary.) <Maintenance: When operating time reaches 2,000 hours.>... -
Page 28: Alarm" And Trouble Shooting
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(28/32) 9. “Alarm” and Trouble shooting 9.1. “Alarm” When Abnormalities of equipment happen, below “Alarm” screen is displayed. detail Alarm contents, Please check below table6. Alarm sign. Table6. Alarm sign... -
Page 29: Trouble Shooting
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(29/32) 9.2. Trouble shooting If you have any problems, please check below Table7. Table7. Trouble shooting Trouble Check Memo Electricity is not Check connecting “Power supply supplied adaptor” , “Power supply cable” and “Control unit”. -
Page 30: Attention
SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(30/32) 10. Attention !!! Do not set and start stretch motion in below setting. There is a possibility of being broken for Machine. Would you please set ”Stretch chamber” on “Stretch chamber holder”, well-balanced. - Page 31 SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(31/32) Appendix 1 1. Chamber coating procedure (1) Coating with fibronectin 1) Dilute fibronectin with PBS to the concentration of 2 to 20 μg/mL. 2) Set a stretch chamber SC4Dea into a dish, add 1 to 1.5 mL of the diluted fibronectin solution 1) to the chamber.
- Page 32 SP_Inst02 /02_20220208 Instruction Manual For ShellPa Pro (Model type : STMP) Page(32/32) 3) Set a stretch chamber SC4Dea into a dish, add 1 to 1.5 mL of the diluted PDL solution 2) to the chamber. Gently tap the dish to completely cover the bottom of the chamber.
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