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Lab-Line 314 Operation Manual

Air-jacketed compact automatic & flo-thru co2 incubators
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OPERATION MANUAL
MODEL NO.
DESIGNERS
AND
A SUBSIDIARY of Barnstead|Thermolyne
1999 North 15th Ave.
, Melrose Park, IL 60160-1491 USA
PHONE: (319) 556-2241 or (800) 522-5463; FAX: (319) 589-0516
AIR-JACKETED COMPACT
AUTOMATIC & FLO-THRU®
CO2 INCUBATORS
AUTOMATIC:
314, 314-1, 314-1CE
(Stainless Steel Interiors)
316, 316-1
(Copper Interiors)
FLO-THRU:
315, 315-1
(Stainless Steel Interiors)
317, 317-1
Copper Interiors)
MANUFACTURERS
1/01
MANUAL NO. 057-561-00
REV. A
LAB-LINE®

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Summary of Contents for Lab-Line 314

  • Page 1 1/01 OPERATION MANUAL MANUAL NO. 057-561-00 REV. A LAB-LINE® AIR-JACKETED COMPACT AUTOMATIC & FLO-THRU® CO2 INCUBATORS MODEL NO. AUTOMATIC: 314, 314-1, 314-1CE (Stainless Steel Interiors) 316, 316-1 (Copper Interiors) FLO-THRU: 315, 315-1 (Stainless Steel Interiors) 317, 317-1 Copper Interiors) DESIGNERS...
  • Page 2 TABLE OF CONTENTS...
  • Page 3 SECTION TITLE Introduction Description Specifications Features Installation Operation Maintenance Replacement Parts Warranty...
  • Page 4 BE ADVISED: IT IS MOST IMPORTANT THAT THE USER FOLLOW INSTALLATION INSTRUCTIONS EXACTLY AS WRITTEN—FAILURE TO DO SO IS LIKELY TO LEAD TO IMPROPER OPERATION, ERRONEOUS CALIBRATIONS AND POSSIBLE DAMAGE TO THE EQUIPMENT. UNDER NO CIRCUMSTANCES SHOULD THE USER ATTEMPT OPERATION WITHOUT THIS INFORMATION. THE FOLLOWING EQUIPMENT IS TO BE SUPPLIED BY THE USER: •...
  • Page 5 Return Goods Authorization Number, will be returned to the sender. To obtain a Return Goods Authorization Number, contact the Customer Relations Department at (800) 522-5463. We hereby certify that the LAB-LINE INSTRUMENTS, INC. product: Model No. and Serial No. which is being forwarded has been properly decontaminated and is free from all toxic hazards, infectious agents, radioactivity and/or other hazards.
  • Page 7 (319) 556-2241 or (800) 522-5463 ALL RIGHT RESERVED The information contained in this manual is the exclusive property of Lab-Line Instruments, Inc., and has been pro- vided solely to enable the users of the equipment described herein to operate and maintain such equipment. Any other use of this information, or the reproduction or transmission of all or any portion of this manual without prior written consent of the manufacturer is expressly prohibited.
  • Page 8 DESCRIPTION 314/316 SERIES AUTOMATIC CO INCUBATORS: BASIC COMPONENTS, AN INITIAL OVERVIEW: 9 10 11 12 1. Control panel 9. Inner glass door, handle shaft 2. CO sensor 10. Inner glass door 11. Inner glass door, handle 3. CO inlet port 12.
  • Page 9 314/316 SERIES AUTOMATIC CO INCUBATORS: (Con’t) BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T) CHAMBER CEILING, INTERIOR CIRCULATING FAN REAR PANEL VIEW, EXTERIOR: 1. GAS INLET PORT 2. CORDSET 3. CIRCUIT BREAKERS 4. RS232 DATA PINS CONNECTOR 5. POWER SWITCH 6. OPTIONAL ALARM RELAY OUTPUT DESCRIPTION: (Con’t)
  • Page 10 A double-door system has a tempered glass inner door that provides unobstructed viewing of the entire chamber. 314 series chambers are constructed of seamless stainless steel for cleaning ease and maintenance; 2 stainless steel shelves are standard—a total capacity is 5 shelves. 316 series incubators feature a fungus and bacteria resistant copper chamber.
  • Page 11 DESCRIPTION: (Con’t) 315/317 SERIES FLO-THRU CO INCUBATORS: BASIC COMPONENTS, AN INITIAL OVERVIEW: 1. AIR & CO2 5. TEMPERATURE SENSOR FLOWMETERS 6. CHAMBER GASKET 2. UNIT FEET 7. INNER GLASS DOOR, HANDLE SHAFT 3. CO2 ACCESS PORT 8. INNER GLASS DOOR 4.
  • Page 12 DESCRIPTION: (Con’t) 315/317 SERIES FLO-THRU CO INCUBATORS: (Con’t) BASIC COMPONENTS, AN INITIAL OVERVIEW: (CON’T) CHAMBER CEILING, INTERIOR CIRCULATING FAN REAR PANEL VIEW, EXTERIOR: 1. CO2 INLET PORT 2. CORDSET...
  • Page 13 DESCRIPTION: (Con’t) 315/317 SERIES FLO-THRU CO INCUBATORS: levels are manually adjusted utilizing flowmeters. Flo-Thru control of concentrations and temperature create a growth environment ideal for tissue cultures, plants, cells, viruses and related organisms. An incoming gas mixture passes through the plenum and is gently circulated throughout the chamber.

  • Page 14: Specifications

    SECTION 3 SPECIFICATIONS 314/316 SERIES AUTOMATIC CO INCUBATORS: ELECTRICAL REQUIREMENTS: 314, 316: 120 V, 60 Hz, 200 Watts, 1.7 Amps, 314-1, 316-1: 230/240 V, 50 Hz, 200 Watts, 0.8 Amps, TEMPERATURE: RANGE: Ambient +5ºC to 60ºC CONTROL: ±0.1ºC at 37ºC.
  • Page 15 315/317 SERIES FLO-THRU CO INCUBATORS: ELECTRICAL REQUIREMENTS: 315, 317: 120 V, 60 Hz, 200 Watts, 1.7 Amps, 315-1, 317-1: 230/240 V, 50 Hz, 200 Watts, 0.8 Amps, TEMPERATURE: RANGE: Slightly above ambient to 60ºC. CONTROL: ±0.5ºC. UNIFORMITY: ±1.0ºC CARBON DIOXIDE RANGE: From 0 to 20% of the mixture.
  • Page 16 FEATURES 314/316 series control panels have a series of command keys that enable users to enter set point values for temperature, carbon dioxide, door heat and to calibrate a unit as required. LED readout provides users with current status information regarding the foregoing parameters.
  • Page 17 TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE 10 11 COMMAND KEY: Pressing the SET key and then the CO allows the user with the Up and Down keys to enter the desired carbon dioxide level in the Incubator. •...
  • Page 18 FEATURES: (Con’t) CONTROL PANEL: (Con’t) TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE 10 11 TEMP COMMAND KEY: Pressing the SET key and then the TEMP command key allows the user with the Up and Down arrow keys to establish the desired set point temperature in the Incubator.
  • Page 19 FEATURES: (Con’t) CONTROL PANEL: (Con’t) TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE 10 11 DOOR HEAT COMMAND KEY: • When the SET key is pressed and then the DOOR-HEAT key, enter the level of heat with the Up and Down keys as a percentage value being applied to the inner glass door.
  • Page 20 FEATURES: (Con’t) CONTROL PANEL: (Con’t) TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE 10 11 HIGH LIMIT THERMOSTAT: This is a back-up high limit temperature thermostat which functions should the microprocessor fail. The high limit thermostat will then control the temperature at slightly above the set point. HIGH LIMIT STATUS LAMP: Indicates when the high limit is active.
  • Page 21 CONTROL PANEL: (Con’t) TEMP DOOR SCAN ºC %CO2 HEAT HOLD HI LIMIT SAMPLE PROPER SEQUENCING IMPORTANT IN OPERATING COMMAND KEYS: To enter commands correctly, it is important to press the keys in SEQUENCE. Do not hold down one key and press another key—the action will not be accepted by the system.
  • Page 22 However, the flashing light will continue. If the condition initiating the alarm remains uncorrected at the end of that time, the audible alarm will resume. PASSWORD, CONTROL PANEL, 314/316 SERIES AUTOMATIC CO INCUBATORS: In the event that you want to prevent unauthorized access to the...
  • Page 23 FEATURES: (Con’t) OPTIONAL ALARM RELAY OUTPUT: REAR OF UNIT, 314/316 SERIES AUTOMATIC CO2 INCUBATORS: The incubator incorporates an output on the rear panel for use as a central alarm voltage-free (“dry contacts”) connection port. This output may be tied in with any central alarm system to give notice of any alarm condition: High or Low Temperature, High or Low CO2, Power Outage or Door Opened.
  • Page 24 FEATURES: (Con’t) CONTROL PANEL, 315/317 SERIES FLO-THRU CO INCUBATORS: TEMPERATURE ºC TEMP SAFETY TEMP CONTROL POWER SAMPLE 1. AIR FLOWMETER: Controls the amount of 6. POWER SWITCH: Turns the unit on and off. incoming air by rotating its control knob. 7.

  • Page 25: Installation

    NOTE: LEAVE UNIT DISCONNECTED WHEN NOT IN USE. HUMIDIFICATION, 314/316 SERIES AUTOMATIC CO2 INCUBATORS: The chamber is designed in such a way that a cold spot is formed causing moisture that is present in the chamber atmosphere to condensate. The condensation is diverted into a humidity trough that is attached to the rear wall of the chamber.
  • Page 26 • 314/316 series incubators, connect tubing with in-line filter in place to the appropriate gas inlet fitting on the back of the unit. 315/317 series incubators require this assembly to be connected to both air and gas inlet fittings.
  • Page 27 HOSE CLAMP 1/4” HOSE BARB #250-331-00 CONNECTIONS (CO2 FITTING) SET PRESSURE AT 4 PSI. PRESSURE REGULATORS AND GAUGES MUST BE INSTALLED ON EACH TANK. CHECK LOCAL GAS SUPPLIER FOR PROPER REGULATORS. 100% CO2 FOR 314/316 MODELS AIR FOR 315/317 MODELS...
  • Page 28 INSTALLATION: (Con’t) STANDARD AND SIPHON TYPE CO GAS CYLINDERS STANDARD TYPE (CORRECT) SIPHON TYPE (INCORRECT) 1. Compressed Gas Association 1. Warning tag indicating that #320 connector. cylinder is siphoning type. 2. Gas head space. 2* Aluminum ring. 3. Liquid CO filled to 68% 3* Gold band.

  • Page 29: Operation

    • Connect unit to power supply meeting the requirements as noted on the nameplate. • Turn the power switch ON. OPERATING BASICS, 314/316 SERIES AUTOMATIC CO INCUBATORS: • Press the SET key first and then the parameter—CO , TEMP, DOOR HEAT—which is to be adjusted.
  • Page 30 • to appear on the readout, enter 4, 2, 1 (i.e. TEMP, CAL and SET) and the password is disabled. SETTING OPERATING TEMPERATURE AND HIGH LIMIT SETPOINT, 314/316 SERIES AUTOMATIC CO INCUBATORS: The unit is factory pre-set at 37ºC and 5.0% CO .
  • Page 31 HIGH LIMIT THERMOSTAT SLIGHTLY CLOCKWISE—MISOPERATION OF THE TEMPERATURE AND POSSIBLY CO2 CONTROL MAY OCCUR IF THE HIGH -LIMIT IS SET TOO CLOSE TO THE SETPOINT. CONDENSATION ON INNER GLASS DOOR, 314/316 SERIES AUTOMATIC CO INCUBATORS: • After the unit has been operating, you can determine what, if any, condensation is forming on the inner glass door.
  • Page 32 TEMPERATURE CALIBRATION, 314/316 SERIES AUTOMATIC CO NCUBATORS: The Incubator is calibrated at the factory. However, if required, the temperature readout may be calibrated by pressing the following keys in sequence: CAL, TEMP. • Use of a calibrated indicating device positioned in the approximate geometric center of the chamber is critical to this procedure.
  • Page 33 LOADING THE INCUBATOR, 315/317 SERIES FLO-THRU CO INCUBATORS: Load the chamber, spacing the items as far apart as possible (for maximum air circulation). AIR/GAS RATIO, 315/317 SERIES FLO-THRU CO INCUBATORS: Set the flowmeters to obtain the required air and CO flow—using the lowest possible CO flow rate will help to conserve gas.
  • Page 34 OPERATION: (Con’t) /AIR FLOW NOMOGRAPH, 315/317 SERIES FLO-THRU CO INCUBATORS: 26 24 22 20 18 16 14 CO2 10 1.5 2 LITERS PER MINUTE, AIR 1. DRAW A VERTICAL LINE FROM THE POINT THAT REPRESENTS THE AIR FLOW ON THE HORIZONTAL AXIS UNTIL IT INTERSECTS A DIAGONAL LINE INDICATING THE REQUIRED VOLUME (%) OF CO2 FLOW;...
  • Page 35 Obtain a sample of CO sufficiently large to meet the requirements of the analytical apparatus or procedure being used. Perform CO analysis. CALIBRATION, 314/316 SERIES AUTOMATIC CO INCUBATORS: In like manner, the CO reading can be calibrated against a known standard such as the Fyrite method of analyzing CO .
  • Page 36 OPERATION: (Con’t) HINTS ON USING THE FYRITE*: The following information is intended to supplement that found in the Fyrite manual and is based on experience working with users of our Incubators. One of the most important aspects of using the Fyrite procedure and one that is frequently overlooked is the condition of the fluid.
  • Page 37 OPERATION: (Con’t) HINTS ON USING THE FYRITE: (Con’t) Inspect the Fyrite filter in the clear plastic casing between the hoses for any contamination or growth. Change it if necessary. CAUTION: MAKE SURE THE FILTER IS MOIST BEFORE YOU TAKE YOUR READING.
  • Page 38 OPERATION: (Con’t) TAKING A FYRITE READING: (Con’t) Depress the plunger and hold it down. It is very important not to allow the plunger to spring up—if this occurs, you will be repeating an already existing reading. It will not matter how many times you pump the squeeze bulb, as long as you hold the plunger down you will introduce only one sample to the Fyrite.

  • Page 39: Maintenance

    SECTION 7 MAINTENANCE BE ADVISED: NOTE: MAKE NO ATTEMPT TO SERVICE OR REPAIR A LAB-LINE PRODUCT UNDER WARRANTY BEFORE CONSULTING YOUR LAB-LINE DEALER. AFTER THE WARRANTY PERIOD, SUCH CONSULTATION IS STILL ADVISED, ESPECIALLY WHEN THE REPAIR MAY BE TECHNICALLY SOPHISTICATED OR DIFFICULT.
  • Page 40 THE ALLOY CALLED STAINLESS: Stainless steel is an alloy of steel with chromium and nickel that increase the metal’s resistance to rust and corrosion. Yet, if not properly cared for, stainless steel can rust and corrode. Exposure to air provides the passivation, or oxide layer coating, for clean stainless by producing a thin, durable chromium-oxide film that forms rapidly on the alloy surface to give stainless its characteristic “stainless”...
  • Page 41 BE ADVISED: NEVER USE THE FOLLOWING ON STAINLESS STEEL: Aqua regia Ferric chloride Iodine Sodium acid Sodium azide Chemical spills, especially those agents listed here, should be removed as soon as possible and the stainless steel surface cleaned with mild soapy water followed by a clean water rinse.
  • Page 42 • Alcohol BE ADVISED: THIS INFORMATION IS INTENDED AS GUIDELINES ONLY AND LAB-LINE INSTRUMENTS, INC. MAKES NO CLAIM AS TO THE SUITABILITY TO ANY PARTICULAR SITUATION. CONSULT YOUR STAFF CHEMIST TO DETERMINE WHAT WOULD BE BEST FOR YOUR STAINLESS STEEL PRODUCT AND LABORATORY.

  • Page 43: Replacement Parts

    SECTION 8 REPLACEMENT PARTS 314/316 SERIES AUTOMATIC CO INCUBATORS: DESCRIPTION PART NUMBER Printed Circuit Board Assy: 018-478-00 Sensor Assy: 018-646-00 Axial Fan: 160-136-00 Schematic 314: 228-553-30 Schematic 314-1: 228-553-29 Schematic, 316: 228-553-21 Schematic, 316-1: 228-553-22 Capacitor: 310-082-00 Circuit Breaker, 5 Amp:...
  • Page 44 Circuit Breaker: 330-118-00 Lens, Red: 360-234-00 Lens, Amber: 360-235-00 Thermostat: 920-301-00 NEED A PART? CALL THE LAB-LINE PARTS HOTLINE. CALL: (319) 556-2241 or (800) 522-5463; FAX: (319) 589-0516. LAB-LINE RESERVES THE RIGHT TO MAKE CHANGES TO SPECIFICATIONS WITHOUT PRIOR NOTICE.

  • Page 45: Warranty

    Lab-Line’s warranty provided hereunder shall be null and void and without further force or effect if there is any (i) repair made to the product by a party other than Lab-Line or its duly authorized service representative, (ii) misuse (including use inconsistent with...
  • Page 46 WARRANTY Page 2 of 2 12 MONTH PARTS WARRANTY: • All Environmental Chambers • Low Temperature B. O. D. Incubators • Animal Study Chamber • Controlled Environment Centers • Biological Work Station • Refrigerators, Freezers • Chromatography Refrigerators (5 year parts warranty on compressor only) •...
  • Page 47 FIRST IN INSTRUMENTS SERVICING SCIENCE, INDUSTRY, RESEARCH AND EDUCATION SINCE 1908.

  • Page 49: Accessory Checklist

    ACCESSORY CHECKLIST The following loose parts and accessories are packed with this unit. Before discarding any packing materials, please make sure that nothing has been overlooked. MODEL NO.: 314, 314-1, 314-1CE, 316, 316-1, 315, 315-1, 317, 317-1 CHECKED BY: ——————— DATE:...

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