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Operation
~~~~~~~~
Centrifuges
A
CAUTION
The
temperature
offset
technique
should
be
used
on
all
runs
to
prevent
~~~~~~~~~~~~~~~
or
freezing
of sample.
This
is particularly
important
at low
set
temperatures
and
at
speeds
lower
than
maximum
rotor
speed.
7.
Turn
the
SPEED
dial
to
the
desired
run
speed
setting.
8.
If
you
want
the
rotor
to
brake
at
the
end
of
the
run,
set
the
brake
ON
by
pressing
the
BRAKE
switch
so
that
the
light
is on.
9.
Press
the
START
switch.
The
centrifuge
will
accelerate
to
the
selected
speed
and
run
for
the
selected
time.
The
run
will
coast
or
brake
to
a stop
when
the
time
expires.
NOTE
If it is necessary
to stop
the
rotor
during
HOLD
or before
the
selected
time
has
elapsed,
always
turn
the
TIME
control
dial
to
STOP.
DO
NOT
stop
the
rotor
by
turning
the
SPEED
dial
to
0 ~~~~
If a power
interruption
occurs
during
a run,
the
run
can
be
continued
by
pressing
the
START
switch.
During
deceleration,
the
chamber
temperature
will
be
controlled
by
the
red
temperature
needle
~~~~~~~~
(standby).
When
the
rotor
has
decelerated
below
100
rpm,
the
DOOR
light
will
turn
on.
The
chamber
door
can
then
be
opened
by
pushing
the
DOOR
button
and
lifting
the
door
handle.
Remove
the
rotor.
3~5.
Rotor
Speed~Temperature
Differential
Compensation
Graphs
To
create
a
graph,
plot
the
selected
temperature
versus
the
actual
sample
temperature
at
a specific
speed
and
ambient
condition.
For
example:
To
achieve
and
maintain
a
+7.5°C
sample
temperature
with
a
~~~
Rotor
at
13
000
rpm,
the
blue
needle
must
be
set
at
approximately
+4°C,
which
is
3°C
colder
than
the
desired
temperature.
NOTE
An
ambient
temperature
of
25°C
or
less
is
required
to
obtain
optimum
cooling
efficiency.
At
higher
ambient
temperatures,
a
lower
operating
speed
may
be
neces¬
sary
to
maintain
sample
temperature.
Test
Run
Procedure
More
accurate
temperature
offset
data
can
be
obtained
by
doing
a
test
run
and
dynamically
calibrating
a
specific
rotor/centrifuge~~desired
speed
combination
and
ambient
condition.
Using
an
~~~~~~~~~~~~
centigrade
thermometer
calibrated
in
1.0°C
increments,
perform
the
procedure
given
on
the
next
page.
3-4
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