Download
Share
Print this page
Thermo Scientific Shandon Excelsior ES Operator's Manual
  1. Manuals
  2. Brands
  3. Thermo Scientific Manuals
  4. Computer Hardware
  5. Shandon Excelsior ES
  6. Operator's manual

Thermo Scientific Shandon Excelsior ES Operator's Manual

Hide thumbs

Advertisement

Quick Links

Download this manual
Shandon Excelsior ES
®
Operator Guide - English
A78410120 Issue 3

Advertisement

loading
Need help?

Need help?

Do you have a question about the Shandon Excelsior ES and is the answer not in the manual?

Questions and answers

Related Manuals for Thermo Scientific Shandon Excelsior ES

Summary of Contents for Thermo Scientific Shandon Excelsior ES

  • Page 1 Shandon Excelsior ES ® Operator Guide - English A78410120 Issue 3...
  • Page 2 Tel: 1-800-522-7270 Fax: +44 (0) 1928 562627 Fax: +1 269-372-2674 www.thermo.com/pathology www.thermo.com/pathology The Shandon Excelsior ES meets the following CE Mark requirements: In Vitro Diagnostic Directive 98/79/EC ® Low Voltage Directive 73/23/EEC, as amended by 93/68/EEC. Shandon Excelsior ES Operator Guide...

  • Page 3: Table Of Contents

    Unload Reagent Groups; Daily Options Menu. Menu Mode Introduction; Set Clock; Processing in Menu Mode; Loading Specimens; Starting the Current Process; Starting a Different Process; Removing Specimens; The Quality Control Screen; Replacing Reagents, Reagent Rotation; Options. Shandon Excelsior ES Operator Guide...
  • Page 4 Clearants; Infiltrants; Flush Reagents; Cleaning Agents. Appendix C Program Examples Routine Overnight Program; Rapid Biopsy Program; Standard Flush Program; Extended Flush Program; Rinse Program. Transportation Instructions Appendix D Packaging the Instrument; Product Return Safety Declaration; Warranty Statement. Index Shandon Excelsior ES Operator Guide...
  • Page 5 Shandon Excelsior ES Operator Guide...
  • Page 6 A warning is given in the document if there is a danger of WARNING personal injury or damage to samples or equipment. Note Notes give more information about a job or instruction but do not form part of the instructions. Shandon Excelsior ES Operator Guide...

  • Page 7: How To Use This Guide

    How to Use This Guide The Shandon Excelsior ES is intended for use in pathology laboratories by operators familiar with tissue processing techniques and laboratory equipment. Before operating the instrument, you should read the section Safety Information on page 8.

  • Page 8: Safety Information

    (Ground) via the mains input supply. • Do not remove any panels or covers. There are no user serviceable parts inside the instrument. • It is important to maintain normal standards of safety and Good Laboratory Practices. Always use common sense and the best known practice when operating the instrument. Shandon Excelsior ES Operator Guide...
  • Page 9 Centre of Gravity 260mm 335mm • Excelsior ES weighs approximately 112 kilograms (246 lbs) when empty. Do not try to move Excelsior on your own - at least two people are needed to move an instrument. Shandon Excelsior ES Operator Guide...
  • Page 10 • Correct maintenance procedures are essential for consistent performance. It is recommended that a Maintenance Contract is taken out with your supplier. • The instrument must be serviced annually by a Thermo- trained engineer in accordance with the instructions contained in the Shandon Excelsior ES Service Manual (A78410101). • Any problems and queries should be referred to your supplier. Shandon Excelsior ES Operator Guide...
  • Page 11 These batteries are classified as, “Class 8, Group III UN No. 2800 Batteries, wet, non-spillable, electric storage, special provision A67”, and meet all requirements of the International Air transport Association (I.A.T.A. Dangerous Goods Regulations). Shandon Excelsior ES Operator Guide...
  • Page 12 Shandon Excelsior ES Operator Guide...

  • Page 13: Introducing Excelsior Es

    Chapter 1 - Introducing Excelsior ES Overview The Shandon Excelsior ES is an enclosed automated tissue processor, combining custom programming with simple operation and reagent management. Screen Reaction Chamber Keypad Reagents Shandon Excelsior ES Operator Guide...
  • Page 14 Exchange Bottles Downdraft Filter Electrical Connections THERMO SHANDON Nominal Ratings: Astmoor, Runcorn, Cheshire, WA7 1PR, UK 100 - 120 V a.c. 50/60 Hz 950VA Fuse Rating: 2 x T10A MADE IN UK DEHYDRANT and CLEARANT Bottles Shandon Excelsior ES Operator Guide...
  • Page 15 The concealed Dehydrant and Clearant Reagent Bottles fixed within are NOT accessible to operators. The Electrical Connections panel is for mains power and monitor, optional printer and remote alarms, data communications, and the instrument POWER ON / OFF switch. Shandon Excelsior ES Operator Guide...
  • Page 16 OK, the Thermo logo is replaced by one or more alert icons. Systems OK ALERT The icons can indicate the need for reagent management and maintenance tasks, hardware issues and instrument faults. See Chapter 6 - Troubleshooting, for more information. Shandon Excelsior ES Operator Guide...
  • Page 17 How You Process You can put specimen cassettes into organised baskets With Excelsior ES arranged in pairs. Basket Lid Specimen Cassette - 37 per half basket Organised Basket Shandon Excelsior ES Operator Guide...
  • Page 18 For larger specimens, use a random basket. Up to 3 Levels of organised baskets Random basket • You put the baskets into the reaction chamber, covering the specimens with a basket lid. Knob Lid Seal Level Sensor Agitator Drive Pins Shandon Excelsior ES Operator Guide...
  • Page 19 Excelsior ES presents a uniquely compact, efficient and highly Excelsior ES informative operator interface, showing: Interface • Reaction chamber status • Program details and menu text • Option keys • Animated graphics showing processing and reagent movement in real time • On-screen help information Customer Information Alters, Logo Text Reaction Chamber Alcohol Quality Reagents Options Shandon Excelsior ES Operator Guide...
  • Page 20 Using a menu is summarised below; and Options • Press a number key, OR highlight one of the sub-menus with the arrow keys • Select one of the five options displayed • Press i to show instructions or more information. Shandon Excelsior ES Operator Guide...
  • Page 21 fixative storage. Dehydrants are stored in six concealed bottles, at positions About Reagents A1 - A6 - Dehydrants with solutions of different concentrations. • operators. You work with one exchange bottle at position Shandon Excelsior ES Operator Guide...
  • Page 22 Shandon Excelsior ES Operator Guide...

  • Page 23: Daily Operations Mode

    [flUSh] displays a red status screen with a current flush program. Use this to run separate flush and rinse cycles outside of routine processing, as may be required. [oPTioNS] displays the Daily options screen, described at the end of this chapter. [QUAliTy CoNTRol] displays the Quality Control screen. Use this for reagent management. Shandon Excelsior ES Operator Guide...
  • Page 24 If too many baskets are shown, press the [lEVEl] key until the display is correct. If you do not select [lEVEl], Excelsior ES assumes a random basket and fills the chamber to the highest level. Shandon Excelsior ES Operator Guide...
  • Page 25 The first reagent is drawn into the reaction chamber, and the status line changes • The monitoring screen shows that the chamber is filled to the correct level. • The baskets are completely covered with reagent. Shandon Excelsior ES Operator Guide...
  • Page 26 Day and Date are correct. If you press too many times and go past the day you need, you can select [PREVioUS DAy]. You can set Day and Date up to seven days in advance. End Time and Day are correct, select When [DElAyED START]. Shandon Excelsior ES Operator Guide...
  • Page 27 The status line on the monitoring screen displays ‘Process is Complete Complete’. You have to: • Drain the reaction chamber • Remove baskets • Wipe away excess wax from the chamber, lid and seal • Flush and rinse the chamber Shandon Excelsior ES Operator Guide...
  • Page 28 From the displayed Flush screen, select [START]. For a different Flush program, select [SELECT FLUSH]. When the monitoring screen shows the Flush program has finished, remove basket (s). Select [OK]. Excelsior ES is ready to start a new process run. Shandon Excelsior ES Operator Guide...
  • Page 29 Menu Mode only. For filters, the counts are displayed in weeks. For A1, X1 and w1 the limit can be based on: • Specific Gravity • Usage counts • Day of the week Shandon Excelsior ES Operator Guide...
  • Page 30 Control screen appears. Then: 1. Remove the reagents and ‘tell’ Excelsior ES. a: Take out the fix1 and fix2 bottles and discard them. b: Highlight fix1 and select [REMOVE]. c: Highlight fix2 and select [REMOVE]. Shandon Excelsior ES Operator Guide...
  • Page 31 (used for the first steps in these processing groups). • Excelsior ES rotates the rest of the reagents. • Excelsior ES uses the fresh reagents in the last step for those process groups which have been rotated. Shandon Excelsior ES Operator Guide...
  • Page 32 A1, X1 and w1. This continues for all subsequent reagents. DAY 2 The next morning, the screen indicates A6, X3, and w3 are empty, whilst Ex1, Ex2 and ww are full as shown. Shandon Excelsior ES Operator Guide...
  • Page 33 [NEW]. Highlight the text under xylene and select [NEW]. 3. Transfer reagents to concealed bottles. • Before continuing, ensure that the wax level is correct. • Running your next process completes the reagent rotation Shandon Excelsior ES Operator Guide...
  • Page 34 A1 and w1. This continues for all subsequent reagents. DAY 2 The next morning, the screen indicates A6 and W3 are empty, whilst Ex1 and WW are full as shown. Shandon Excelsior ES Operator Guide...
  • Page 35 Place fresh 100 % alcohol into position • In the Quality Control screen highlight the text under alcohol and select [NEW]. 3. Before continuing, ensure that the wax level is correct. • Running your next process completes the reagent rotation. Shandon Excelsior ES Operator Guide...

  • Page 36: Daily Options Menu

    If you are unclear about any of these details, consult your IT Manager. Open the upper-right door, and insert the disc. Select [DOWNLOAD]. When the lamp is no longer illuminated, the download is complete and you can remove the floppy disc. Shandon Excelsior ES Operator Guide...
  • Page 37 You have Summary • Processed your tissue specimens. • Flushed the instrument. • Replaced fixative and flush reagents. • Managed the replacement of the dehydrants, clearants and wax across twelve containers, using one rotation procedure. Shandon Excelsior ES Operator Guide...
  • Page 38 Check the operation of the switch. When the O side ISOLATION is pressed (OFF), the battery fault icon should be SWITCH displayed. When the I side of the switch is pressed (ON), the icon should disappear. Carry out this check every month. Shandon Excelsior ES Operator Guide...

  • Page 39: Instrument Setup Menu

    This is explained in section 9. Unload Reagent Groups. This chapter describes practical use of the menu sections and the selection keys to achieve associated instrument tasks. From Main Menu, select 1. Instrument Setup. Shandon Excelsior ES Operator Guide...
  • Page 40 Select [SET CLOCK] or [SET DATE] to highlight the settings and display the [SET] options key. Use the number keys to change the parameters. When the clock is correct, Select [SET]. Select [12/24 HOUR CLOCK] and [DAY<>MONTH] keys to change the display format. Shandon Excelsior ES Operator Guide...
  • Page 41 Wax is stored in the molten state, so the ambient temperature option is not applicable. • The available range is 45 °C to 65 °C. • Thermo recommends wax storage temperature 4 °C above melting point. s Shandon Excelsior ES Operator Guide...
  • Page 42 [SELECT], OR move down the list to a blank position. Go to empty position Key becomes available A new key is displayed, [CHANGE NAME]. Select this to display a QWERTY keyboard on the screen, as shown. Shandon Excelsior ES Operator Guide...
  • Page 43 Select [CAPS LOCK] to return to plain letters. Repeat for all characters required. Press EXIT to set the Reagent Name and return to the previous menu. Move down to the next item in the list. Shandon Excelsior ES Operator Guide...
  • Page 44 Move down the reagent list and load reagents in the order - wax, flush reagents, dehydrants, clearants. Fixatives are loaded from the Quality Control screen. Loading Wax Refer to the diagram below for wax loading. Wax Bath Inside Door Inside Lever Wax Bath Main Door Waste Wax Tray Main Door Lever Metal Cover Shandon Excelsior ES Operator Guide...
  • Page 45 Pull down the inside door to the open position - this lets you return both doors together, to the inclined position. Attach the wax fill chute, as shown. Wax Fill Chute Wax Pellets Only Starting from the right, carefully fill each wax bath with wax pellets. Shandon Excelsior ES Operator Guide...
  • Page 46 If the level is too low, add more wax pellets. Fit a new liner into the waste wax tray, then replace the metal wax cover, waste wax tray and lid. Close the wax bath main door. Waste Wax Lid Liner Waste Wax Tray Shandon Excelsior ES Operator Guide...
  • Page 47 Exchange Exchange Right Front Right Back GREEN GREEN Centre Back Centre Front BLUE BLUE Flush Right Bottom GREEN Ensure that each pipe is: • Fully inserted in the bottle • Not bent, twisted or kinked Shandon Excelsior ES Operator Guide...
  • Page 48 The screen moves to f2 flush 2, highlighting the text and outlining the bottle. Take a new bottle of the second flush reagent, e.g. alcohol. Put the bottle on top of the Flush 3 bottle, in front of bottle F1 and insert the BLUE reagent tube (with cap). Select [CONFIRM LOADED]. Shandon Excelsior ES Operator Guide...
  • Page 49 Ex2, and insert the Put the bottle of clearant in position RED tube (with cap). Select [CONFIRM LOADED] to start the clearant reagent transfer. Repeat the above steps to load clearants X 2 and X 3. Shandon Excelsior ES Operator Guide...
  • Page 50 [NEW] to be selected for fix1 and fix2 (if fix2 is to be used). When this happens, fix1 and fix2, inserting the Put fixative bottles in positions GREEN reagent tubes fully, together with GREEN caps. Select [NEW]. Exit the Quality Control screen. Shandon Excelsior ES Operator Guide...
  • Page 51 1. Highlight a user-defined slot in the program title list. User-defined Select [CHANGE TITLE] to display the QWERTY Program Title keyboard. Follow the procedure on page 43 to enter the new title, and press to enter the Define Program screen. EXIT Shandon Excelsior ES Operator Guide...
  • Page 52 Programs Define Program screen, with program steps and parameters; REAGENT HOLD / USE TIME DRAIN VACUUM STIR Time to Reaction Basket Step Reagent Process Temperature Drain Chamber Contra- Name Time Chamber Vacuum rotation Program steps Shandon Excelsior ES Operator Guide...
  • Page 53 The ‘Use’ temperature can be entered here using the keypad. The ‘Time’ column shows hours and minutes for each Time processing step - the maximum is 99:59. Highlight the time and change by entering a number with the number keys. Shandon Excelsior ES Operator Guide...
  • Page 54 Highlight the required program from the list. When the correct program is highlighted, select [COPY NOW]. Select [RESTORE DEFAULTS] to restore the original factory programs. This eliminates any user-defined programs and changes to the default programs Shandon Excelsior ES Operator Guide...
  • Page 55 - the instrument does NOT count the cassettes. Level Key Available In Daily Operations mode, a [LEVEL] option key is displayed in Daily Operations when the reaction chamber lid is opened. Shandon Excelsior ES Operator Guide...
  • Page 56 Available Until the number keys to enter the time for Excelsior ES to stop displaying daytime programs. [NONE SELECTED] - You can choose not to have a daytime program appear when the lid is opened. Shandon Excelsior ES Operator Guide...
  • Page 57 Quality Control screen at the start of a process. This is for Excelsior ES to inform the operator: • The defined reagent usage limit has been reached • They should rotate reagents. Shandon Excelsior ES Operator Guide...
  • Page 58 • It is possible to set more than one trigger, but for correct use of the system you should only set Excelsior ES for one of the rotation trigger options. Shandon Excelsior ES Operator Guide...
  • Page 59 • Repeat this process for the other wax baths. • When the last wax has been unloaded, the screen instructions will prompt you to select [START] for a flush run. Shandon Excelsior ES Operator Guide...
  • Page 60 Remove the Fix 1 and Fix 2 bottles from the cabinet and select [UNLOADED]. • Dispose of waste reagents in accordance with local procedures and regulations. • The unload procedure will continue by highlighting the current dehydrant bottle and the Ex 1 position bottle. Shandon Excelsior ES Operator Guide...
  • Page 61 Place another empty bottle in the Ex 1 position and follow the screen instructions to remove the remaining dehydrants. • When the last dehydrant has been unloaded, the screen returns to the Instrument Setup menu. Shandon Excelsior ES Operator Guide...
  • Page 62 Shandon Excelsior ES Operator Guide...

  • Page 63: Menu Mode

    Processing in Menu Mode is the same as for Daily Operations, with the exception that the [lEVEl] key option is permanently enabled. Procedures are given below, with reference to selection of the [lEVEl] key, where appropriate. Shandon Excelsior ES Operator Guide...
  • Page 64 If too many baskets are shown, press the [lEVEl] key until the display is correct. If you do not select [lEVEl], Excelsior ES assumes a random basket and fills the chamber to the highest level. Shandon Excelsior ES Operator Guide...
  • Page 65 The first reagent is drawn into the reaction chamber, and the status line changes • The monitoring screen shows that the chamber is filled to the correct level. • The baskets are completely covered with reagent. Shandon Excelsior ES Operator Guide...
  • Page 66 If you press too many times and go past the day you need, you can select [PREVioUS DAy]. You can set Day and Date up to seven days in advance. 4. When End Time and Day are correct, select [DElAyED START]. Shandon Excelsior ES Operator Guide...
  • Page 67 1. Select [DRAIN ALL CHAMBER] and wait till draining Specimens stops. Open the lid and remove basket(s). If baskets are still shown in the chamber, press the [LEVEL] key to clear them. 3. Wipe out the reaction chamber. Shandon Excelsior ES Operator Guide...
  • Page 68 From the displayed Flush screen, select different Flush program, select [SELECT FLUSH]. When the monitoring screen shows the Flush program has finished, remove basket (s). [oK]. Select Excelsior ES is ready to start a new process run. Shandon Excelsior ES Operator Guide...
  • Page 69 The limits are set in the Quality Control screen, when in Menu Mode. For filters, the counts are displayed in weeks. For A1, X1 and w1 the limit can be based on: • Specific Gravity • Usage counts Shandon Excelsior ES Operator Guide...
  • Page 70 Open the lower doors - the Quality Control screen appears. Then: 1. Remove the reagents and ‘tell’ Excelsior ES. a. Take out the fix1 and fix2 bottles and discard them. b. Highlight fix1 and select [REMoVE]. Shandon Excelsior ES Operator Guide...
  • Page 71 (used for the first steps in these processing groups). • Excelsior ES rotates the rest of the reagents. • Excelsior ES uses the fresh reagents in the last step for those process groups which have been rotated. Shandon Excelsior ES Operator Guide...
  • Page 72 A1, X1 and w1. This continues for all subsequent reagents. DAY 2 The next morning, the screen indicates A6, X3, and w3 are empty, whilst Ex1, Ex2 and ww are full as shown. Shandon Excelsior ES Operator Guide...
  • Page 73 In the Quality Control screen highlight the text under alcohol and select [NEw]. Highlight the text under xylene and select [NEw]. 3. Transfer reagents to concealed bottles. Before continuing, ensure that the wax level is correct. Running your next process completes the reagent rotation. Shandon Excelsior ES Operator Guide...
  • Page 74 A1 and w1. This continues for all subsequent reagents. DAY 2 The next morning, the screen indicates A6 and w3 are empty, whilst Ex 1 and ww are full as shown. Shandon Excelsior ES Operator Guide...
  • Page 75 Place fresh 100 % alcohol into position Ex1. In the Quality Control screen highlight the text under alcohol and select [NEw]. 3. Before continuing, ensure that the wax level is correct. Running your next process completes the reagent rotation. Shandon Excelsior ES Operator Guide...
  • Page 76 This takes you to Daily Operations mode. Daily Operations Highlight the language you want and select [SELECT]. Select Language Set Time and Date Sets Time and Date as in Chapter 3, Instrument Setup. The Access Codes screen is shown below. Shandon Excelsior ES Operator Guide...
  • Page 77 You can set Access Codes for different users, to define who can perform different functions with Excelsior ES. To change the access code groups, Enter the Administrator access code on the number keys. You can now set up to 7 user groups. Shandon Excelsior ES Operator Guide...
  • Page 78 To enable access codes when the set up is complete press Printout Options The Printout Options screen is shown. You can print • Hard copies to a printer • To the Excelsior ES screen • To the Excelsior ES disc drive Shandon Excelsior ES Operator Guide...
  • Page 79 1. Highlight an option in From the list. 2. Insert a floppy disc into the disc drive. 3. Select [SElECT]. Excelsior ES can save programs and instrument setup information on standard 3.5” floppy discs. New languages and features can also be loaded. The Audio / Remote Alarms screen is shown with default Audio / Remote Alarms settings. Shandon Excelsior ES Operator Guide...
  • Page 80 D-connector, to report events listed on the LIMS screen. Interface Use this option to bring any concealed reagent or wax into the Reagent and Wax Checks reaction chamber, outside of processing. The Reagent and Wax Inspection Screen is shown. Shandon Excelsior ES Operator Guide...
  • Page 81 3 when the instrument is loaded from 5L bottles. If using 3.8 L (1 US Gallon) bottles, the chamber fluid level is just below sensor 3. • Add more reagent. Select [RETURN REAGENT] to drain the chamber into the same bottle. Or, Shandon Excelsior ES Operator Guide...
  • Page 82 Note After inspecting xylene or equivalent reagents, the chamber must be flushed before processing. Highlight the relevant wax bath. Inspecting a Wax Select [iNSPECT wAX] to move the wax into the reaction chamber. Shandon Excelsior ES Operator Guide...
  • Page 83 The screen instructions ask you to confirm that the wax waste tray is empty. Select [CoNfiRM loADED] to drain the wax from the reaction chamber to the wax waste tray. Discard the used wax in accordance with local procedures and regulations. Shandon Excelsior ES Operator Guide...
  • Page 84 When the reaction chamber is empty, the screen highlights the empty wax bath and the second key shows [START hEATiNG]. Pour new wax pellets into the wax tray and press [START hEATiNG]. Shandon Excelsior ES Operator Guide...
  • Page 85 Loaded ALWAYS KEEP THE INSTRUMENT LEVEL WHEN IT IS LOADED WITH WAX AND REAGENTS. MOVE IT SLOWLY TO PREVENT WAX SPILLAGE. s ALWAYS DISCONNECT AND REMOVE THE MONITOR BEFORE THE INSTRUMENT IS MOVED. s Shandon Excelsior ES Operator Guide...
  • Page 86 If necessary, adjust the front castors. Loosen the locknut (a wrench is provided), then turn the castor with the adjustment rod provided. When the instrument is level, tighten the locknut. Mop up the water and dry the reaction chamber. Shandon Excelsior ES Operator Guide...
  • Page 87 Connecting a Printer shown on the electrical connections panel. Printers used should comply with IEC950 or IEC1010-1. Connections for Remote Alarms 1 and 2 are shown, Connecting a Remote indicating ‘normally open’ and ‘normally closed’ connectors. Alarm Shandon Excelsior ES Operator Guide...
  • Page 88 1. Open the top door and remove the metal baffle plate. 2. Slide out the filters and remove the plastic wrappers. 3. Replace the filters in the correct positions, so that the airflow arrow on the filter points up. 4. Replace the baffle plate and close the top door. Upper Filter Baffle Lower Filter Shandon Excelsior ES Operator Guide...
  • Page 89 The optional vent adaptors allow fumes to be extracted into a Vent Adaptors fume cupboard or hood, or vented to the outside atmosphere. DO NOT USE THE VENT ADAPTORS TO EXTRACT THE FUMES THROUGH THE BUILDING HVAC SYSTEM OR A COMMON SITE EXTRACTION SYSTEM. s Shandon Excelsior ES Operator Guide...
  • Page 90 If another plug is required, it is necessary for a technically-competent person to remove the moulded plug from the white power cord (UK-style plug) and fit a suitably rated, fused plug using the wiring convention shown below: Shandon Excelsior ES Operator Guide...
  • Page 91 Highlight the required language and select [SELECT] The screen displays the battery isolation switch and heater reset alert icons. The icons must be cleared, before any further operations: 1. Turn ON the battery isolation switch. Shandon Excelsior ES Operator Guide...
  • Page 92 ON at all times. Press the Standby key[ ] to turn off the screen and lock the keyboard - processing continues. 1 2 3 4 5 < > ENTER EXIT 6 7 8 9 0 CANCEL Note Shandon Excelsior ES Operator Guide...
  • Page 93 2. Press the O (OFF) side of the main I/O power switch to switch off Excelsior ES. 3. Wait approximately 45 seconds. 4. Press the O (OFF) side of the Battery Isolation Switch to isolate the battery. Shandon Excelsior ES Operator Guide...
  • Page 94 • TABLE 4 on page 102 answers Frequently Asked Questions. Back up power switch OFF Heater trip Hardware Fault Demo Mode Remote communications Access code unlocked Alarm sounded Door open Reagent Quality Control issue Shandon Excelsior ES Operator Guide...
  • Page 95 Fault Status screen - see below for details. Remote Alarm. A remote alarm has sounded. The icon shows whether it is Alarm 1 or Alarm 2. Press on the keypad to silence the alarm. Shandon Excelsior ES Operator Guide...
  • Page 96 If the status is fAil, you can select [RETRy]. These selections clear the alert icon, and you can try to continue processing. If the problem persists, contact your Thermo Distributor. Reaction chamber underfill and overfill events are displayed here. Underfills and Recovery Shandon Excelsior ES Operator Guide...
  • Page 97 The second short Return to the Load Reagents reagents, a Process flush routine at menu, and complete the se- Start screen is not the end of the load quence. displayed. sequence has not been run. Shandon Excelsior ES Operator Guide...
  • Page 98 If there is an instrument malfunction remove ALL specimens from the reaction chamber. If it is necessary to release the chamber vacuum, open the top right door, remove the metal plate and pull the vacuum release knob (see pgs 14-15). Contact Thermo Service. Shandon Excelsior ES Operator Guide...
  • Page 99 2 Use vacuum • No support when sec- 3 Bisect if encapsulated speci- tioned men or produce thinner block • Section breaks up on the 4 Reinfiltrate. Use vacuum to water bath promote infiltration. Shandon Excelsior ES Operator Guide...
  • Page 100 2 Decrease time and/or steps 3 Check temperature reading with thermometer 4 Segregate small, delicate specimens 5 Soak for shirt time in chilled water, do not trim away hydrated area and section slowly. Shandon Excelsior ES Operator Guide...
  • Page 101 Shandon Excelsior ES Operator Guide...
  • Page 102 Where are the keys to From Menu Mode, select Instrument Setup, Rotation change the alcohol qual- Management. Highlight the Alcohol Quality line. Two ity rotation setting? selection keys [Up] and [Down] are displayed. Shandon Excelsior ES Operator Guide...
  • Page 103 Shandon Excelsior ES Operator Guide...
  • Page 104 Shandon Excelsior ES Operator Guide...
  • Page 105 Maintenance Normal standards of laboratory hygiene and routine maintenance Introduction procedures are all that is necessary to keep Shandon Excelsior ES in good and serviceable condition. if hAZARDoUS MATERiAl iS SPliT oN, oR iNSiDE, ThE iNSTRUMENT, yoU MUST CARRy oUT ThE APPRoPRiATE DECoNTAMiNATioN.
  • Page 106 These are the reagent supplier’s bottles in the front cabinet. (Note that 2 empty F1, F2 Bottles 5 litre bottles are supplied for the fix 1 and fix 2 positions). To remove the bottles, carefully remove the dip tubes from the reagent bottles Shandon Excelsior ES Operator Guide...
  • Page 107 Fit the green drain tube into the open pipe at the rear of the Flush 3 Water Bottle. Make sure the tube is fully inserted into the Water Bottle so that it sits on the bottom of the bottle and that there are no kinks in the flexible section Shandon Excelsior ES Operator Guide...
  • Page 108 Keep the waste wax tray safely for reuse. Ensure that there is always a waste wax tray in the instrument with a correctly WARNING Shandon Excelsior ES Operator Guide...
  • Page 109 THE LID SEAL AND TOP SURFACE OF THE REACTION CHAM- BER MUST BE KEPT CLEAR OF WAX FOR THE INSTRUMENT TO OPERATE CORRECTLY. THE LID SEAL CANNOT BE REMOVED FOR CLEANING. - DO NOT TRY TO REMOVE IT. Shandon Excelsior ES Operator Guide...
  • Page 110 Close the Reaction Chamber Lid and perform a flush to rinse the Cham- ber. Note Wipe the Reaction Chamber after every flush and check for any contami- nation. Evidence of wax may indicate that the flush reagents need to be changed. Cleaning the Ensure the instrument is powered OFF. Disconnect the monitor from the Monitor instrument. Wipe the monitor gently with a soft damp cloth. Shandon Excelsior ES Operator Guide...
  • Page 111 Area. To access the spillage containment area, lift and remove the metal shelf in the base of the Reagent Storage Area. Clean any liquid from this area and dispose of in accordance with any local procedures or regulations. Replace the metal shelf and the reagent bottles. Shandon Excelsior ES Operator Guide...
  • Page 112 Shandon Excelsior ES Operator Guide...
  • Page 113 ± 10% of nominal voltage Internal batteries (Yuasa NP12-12) 12V, 12Ah valve regulated sealed lead-acid type rechargeable battery Remote Alarm 3A, 240 VAC operation; Interface Connections non powered output; LIMS Serial RS232 Netmon Serial RS232 Printer Centronics Parallel; Shandon Excelsior ES Operator Guide...
  • Page 114 Altitude up to 2000m (6,500 feet) Pollution degree 2 Over voltage categoryII A78400006 Thermo Part Number Centre of gravity position for a fully laden instrument (see also page 5) Centre of Gravity Position 260mm 335mm Shandon Excelsior ES Operator Guide...
  • Page 115 Wax Waste Tray Kit, A78410027 comprising Wax Waste Tray Wax Waste Tray Lid Wax Waste Liners AP14747 Door Keys 1 set AP14733 General Operator Guide A78410100 Screen Information Cards 1 set A784-1002 Service Manual A78410101 Spatula P09046 Shandon Excelsior ES Operator Guide...
  • Page 116 Ready to use (209 litres /55 gal) 9990509 Wrench for 19 litre carboy B1000722 PARAffiN wAX Histoplast (10 kg bag) 6774060 Histoplast (2.5 kg box) 6774006 Paraplast® (1 kg bag) 501006 Paraplast® Plus (1 kg bag) 502004 Shandon Excelsior ES Operator Guide...
  • Page 117 A full range of reagents and cassettes is listed in the Thermo catalogue. Formal-Fixx Glyo-Fixx and HistosolveAdobe Garamond Pro are trademarks of Thermo Scientific; Paraplast® is a registered trademark of Sherwood Medical; Peel-A-Way® is a registered trademark of Polysciences. Shandon Excelsior ES Operator Guide...
  • Page 118 Optional accessories considered subject to the In Vitro Diagnostics Directive (IVDD) are specifically identified on this Declaration of Conformity. Further supplies of standard accessories are treated as spares. Convenience aids offered as accessories are not subject to the IVDD. Shandon Excelsior ES Operator Guide...
  • Page 119 Approved Reagents Introduction This Section lists all the reagents specified by Thermo for use with the Shandon Excelsior ES tissue processor. If you want to use a reagent not included in this list, contact your Thermo agent for advice. WARNING Do not use any reagents containing calcium chloride.s...
  • Page 120 Alcohols from dehydrants list Water Cleaning Agents Dilute detergent Water Shandon Excelsior ES Operator Guide...
  • Page 121 Rapid Biopsy HOLD / USE REAGENT TIME DRAIN VACUUM STIR Program Formalin 00:30 Formalin 00:30 Alcohol 01:00 Alcohol 01:00 Alcohol 01:00 Alcohol 01:00 Alcohol 01:00 Alcohol 01:00 Xylene 01:00 Xylene 01:00 Xylene 01:00 01:20 01:20 01:20 Shandon Excelsior ES Operator Guide...
  • Page 122 Extended Flush REAGENT HOLD / USE TIME DRAIN VACUUM STIR Program Flush 1 Amb/60 00:30 Flush 2 Amb/45 00:05 Flush 3 00:02 Rinse Program REAGENT HOLD / USE TIME DRAIN VACUUM STIR Flush 3 00:02 Shandon Excelsior ES Operator Guide...
  • Page 123 EMPTY AND 200KG (440LB) WHEN FULL. AT LEAST TWO PEOPLE ARE NEEDED TO SAFELY MOVE THE INSTRUMENT. s 3. Carefully wheel the Shandon Excelsior ES up the ramp until it sits securely in the base. Shandon Excelsior ES Operator Guide...
  • Page 124 Make sure that the foam pieces are in place. Raise the ramp and fit the Inner Cover. Secure the ramp to the Inner Cover with tape. Fit the Outer Cover. Fit the lid over the Outer Cover. Shandon Excelsior ES Operator Guide...
  • Page 125 Secure the packaging by two bands through the palette and over the lid , and one band around the bottom of the packaging. WHEN TRANSPORTING EXCELSIOR ES AFTER PACKAGING ENSURE THAT THE INSTRUMENT IS KEPT UPRIGHT AT ALL TIMES. s Shandon Excelsior ES Operator Guide...
  • Page 126 Thermo Fisher Scientific, Anatomical Pathology, 4481 Campus Drive, Kalamazoo, MI 49008, USA Tel: 1-800-522-7270, Fax: +1 269-372-2674, www.thermo.com/pathology Thermo Fisher Scientific, Anatomical Pathology, 93-96 Chadwick Road, Astmoor, Runcorn, Cheshire, WA7 1PR, UK Tel: +44 (0)1928 562600, Fax: +44 (0)1928 562627, www.thermo.com/pathology Shandon Excelsior ES Operator Guide...
  • Page 127 Please note that your warranty may be invalidated if: • The instrument is modified in any way. • Accessories and reagents are used that are not approved by Thermo, or; • The instrument is not operated or maintained in accordance with the instructions in this Operator Guide. Shandon Excelsior ES Operator Guide...
  • Page 128 20 add notes 51 Conformity change notes 52 declaration of 118 drain 54 contact addresses 2 setting reaction chamber vacuum 54 CONTROLS AND SCREEN Electrical Specification 113, 114 LAYOUT Emergency vacuum release 99 Screen Displays Shandon Excelsior ES Operator Guide...
  • Page 129 28, 68 fixed reagents 49, 50, 89 Fuse mains plug 90 mains input 114 Maintenance 10 regular 105 Monitor 87 Heater reset switch 91 Height 113 Notes add Notes feature 52 Information button Inspect Shandon Excelsior ES Operator Guide...
  • Page 130 Reaction chamber instrument function - table 1 98 heater trip 96 processing problems Reagent rotation 32, 72 hard, brittle tissue - table 3 101 control all groups 34, 74 increasing process time 100 rotation management 57 Shandon Excelsior ES Operator Guide...
  • Page 131 46 paraffin wax pellets 21 adding 33, 35, 73, 75 storage temperature 41 waste wax container WW removing 32, 34, 72, 74 wax rotation 32, 72 Wax Waste Tray 108 Wiring details 91 Shandon Excelsior ES Operator Guide...