Kikkoman LUMITESTER C-110 Operation Manual
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Kikkoman LUMITESTER C-110 Operation Manual

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OPERATION MANUAL
Thank you for purchasing the LUMITESTER C-110.
To use your LUMITESTER C-110 safely and correctly,
read this operation manual carefully and follows the instructions.
Kikkoman Corporation
Ⓒ2009 Kikkoman Corp. (04-1)

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  • Page 1 OPERATION MANUAL Thank you for purchasing the LUMITESTER C-110. To use your LUMITESTER C-110 safely and correctly, read this operation manual carefully and follows the instructions. Kikkoman Corporation Ⓒ2009 Kikkoman Corp. (04-1)
  • Page 2 This symbol indicates a risk of smoke emission or fire. Explosive hazard This symbol indicates a risk of explosion. Toxic hazard This symbol indicates a toxic substance risk. Corrosion hazard This symbol indicates a risk of corrosion. Ⓒ2009 Kikkoman Corp. (04-1)
  • Page 3 Doing so may cause electrical shock, fire or malfunction. Do not expose this unit to water and do not handle it with wet hands. This unit is not water-proof, so water or moisture may cause electrical shock, fire or malfunction. Ⓒ2009 Kikkoman Corp. (04-1)
  • Page 4 • Avoid using this unit in locations directly exposed to air flow from an air conditioner or fan. • Do not place any object on this unit. • Be careful to prevent electrostatic charge buildup on the sample tube. Static electricity may cause an increase the measurement data. Ⓒ2009 Kikkoman Corp. (04-1)
  • Page 5 Doing so may cause this unit to explode internally. Do not store this unit in locations where water or chemicals may penetrate inside this unit. A short-circuit may occur if water or chemicals penetrate inside this unit, causing fire or electrical shock. Ⓒ2009 Kikkoman Corp. (04-1)
  • Page 6 When disposing of this unit and reagents be sure to comply with local regulations regarding industrial waste disposal. Consult the local regulations in your area for detailed information. WARNING Do not place this unit in fire or attempt to burn it. Doing so may cause the unit to explode internally. Ⓒ2009 Kikkoman Corp. (04-1)
  • Page 7 Ⓒ2009 Kikkoman Corp. (04-1)

  • Page 8: Table Of Contents

    6-2 ATP amount (pg) ..................24 6-3 Number of cells (CELL) ................24 7. Computer Interface....................25 8. Maintenance ......................26 8-1 Cleaning the measurement chamber ............26 8-2 Replacing the rechargeable battery pack ...........26 9. Specifications ....................27 10. Dimensions......................28 11. After Sales Service ....................29 Ⓒ2009 Kikkoman Corp. (04-1)

  • Page 9: Overview

    LUMITESTER C-110 measures low-level light by photon counting method that ensures high sensitivity and low noise. The LUMITESTER C-110 operates with either a rechargeable battery or AC adapter, allowing wide flexibility with regard to measurement locations. Operation is very simple. All you need to begin measurement is just insert a sample tube and press the start key.

  • Page 10: Part Names And Functions

    Accessories Use this AC adapter when operating the LUMITESTER AC adapter from the AC line. RS-232C cable Use this cable to connect to a printer or computer. Contains utility software. Ⓒ Kikkoman Corp. (04—1)
  • Page 11 Press this key to enter the function routine mode. (3) <Scroll> key Use this key to scroll the measurement data or select test conditions. (4) <ENTER> key Press this key to begin measurement or enable data entry. Ⓒ Kikkoman Corp. (04—1)

  • Page 12: Power

    <When a battery mark appears on the display panel> The remaining battery power is low, so recharge the battery. <CAUTION !! Always use the AC adapter supplied with the LUMITESTER.> If an AC adapter other than supplied is used, the LUMITESTER may malfunction. Ⓒ Kikkoman Corp. (04—1)

  • Page 13: Operating Procedure

    Operating Procedure Basic operation This section explains basic operations of the LUMITESTER C-110. The LUMITESTER C-110 can be operated in the following two modes for making measurements. • STANDARD mode Measurement results are displayed in units of RLU (relative light unit), M, pg or CELL which are selectable.
  • Page 14 • Press the <POWER> key to turn the power off. * If you attempt to turn off the power while a sample tube is still in the LUMITESTER, a beep sounds, so open the cover and remove the sample tube. Ⓒ Kikkoman Corp. (04—1)

  • Page 15: Advanced Mode Measurement

    • Press the <ENTER> key. Measurement starts under the start delay and measurement time conditions which are set by "MEASURING TIME" function routine. • Wait until measurement is over (until the center digit counts MEASURING ＃XXXX down to “0”). ５ Ⓒ Kikkoman Corp. (04—1)
  • Page 16 • Press the <POWER> key to turn the power off. * If you attempt to turn off the power while a sample tube is still in the LUMITESTER, a beep sounds, so open the cover and remove the sample tube. Ⓒ Kikkoman Corp. (04—1)

  • Page 17: Function Routines

    Function routines The LUMITESTER uses function routines to clear data or make various settings. First of all, familiarize yourself with the basic operation flow for making condition settings and selections. LUMITESTER C-110 Menu List STANDARD MODE ADVANCED MODE FUNCTION ROUTINE...

  • Page 18: Print [Print Out]

    ・ After selecting the date, press the <F> key. YES=ENTER ・ When a conformation screen appears, press the <ENTER> NO=FUNCTION key to start printing. ＥＮＴＥＲ ・ To abort printing, press the <F> key. PRINTING DATA STOP[ＦＵＮＣＴＩＯＮ] MAIN MENU Ⓒ Kikkoman Corp. (04—1)
  • Page 19 ARE YOU SURE？ CAUTION ! Be sure to turn off the power when connecting or YES=ENTER NO=FUNCTION disconnecting a printer from the LIMITESTER. ＥＮＴＥＲ PRINTING DATA STOP[ＦＵＮＣＴＩＯＮ] MAIN MENU Ⓒ Kikkoman Corp. (04—1)

  • Page 20: Data Clear [Clear All Data]

    • In the CLEAR ALL DATA function, press the <ENTER> PRINT OUT key to clear the data. ＊CLEAR ALL DATA TRANS ALL DATA SET-UP • To cancel data clear, press the <F> key. ＥＮＴＥＲ CLEAR ALL DATA WITH ENTER KEY ＥＮＴＥＲ MAIN MENU Ⓒ Kikkoman Corp. (04—1)

  • Page 21: Rs-232C Transmission [Trans All Data]

    <ENTER> key. TRANS ALL DATA ＊DATA • To stop transmission, press the <F> key. CAL.CURVE * For details on RS-232C, refer to Chapter 7, Computer ＥＮＴＥＲ Interface". TRANSFER STOP[ＦＵＮＣＴＩＯＮ] MAIN MENU Ⓒ Kikkoman Corp. (04—1)

  • Page 22: Various Settings [Set-Up]

    LANGUAGE This selects the display language, Japanese or English. ＢＥＥＰ ＊ＬＡＮＧＵＡＧＥ Procedure ＤＡＴＥ ＆ ＴＩＭＥ • Select the language using the scroll keys. ＣＯＵＮＴＲＹ • Press the <ENTER> key. ＥＮＴＥＲ ＬＡＮＧＵＡＧＥ ＊ＪＡＰＡＮＥＳＥ ＥＮＧＬＩＳＨ ＥＮＴＥＲ MAIN MENU Ⓒ Kikkoman Corp. (04—1)
  • Page 23 ＢＥＥＰ following three styles. ＬＡＮＧＵＡＧＥ ＤＡＴＥ ＆ ＴＩＭＥ JAPAN yy.mm.dd ＊ＣＯＵＮＴＲＹ mm.dd.yy ＥＮＴＥＲ EUROPE dd.mm.yy ＣＯＵＮＴＲＹ Operation ＊ＪＡＰＡＮ • Select the country using the scroll keys. ＵＳＡ ＥＵＲＯＰＥ • Press the <ENTER> key. ＥＮＴＥＲ MAIN MENU Ⓒ Kikkoman Corp. (04—1)

  • Page 24: Display Unit Selection [Choose A Unit]

    Displays values that were converted to the number of cells. Procedure Ｆ • Select the desired unit and press the <ENTER> key. CHOOSE A UNIT ＊ CHOOSE ATP/CELL INPUT ATP/CELL INPUT COEF.(pg) ENTER ＊ＲＬＵ Ｍ ｐｇ ＣＥＬＬ ＥＮＴＥＲ MAIN MENU Ⓒ Kikkoman Corp. (04—1)

  • Page 25: Atp/Cell Coefficient Selection [Choose Atp/Cell]

    • Select the type of cell using the scroll keys Ｂ １．００Ｅ－１８ｍｏｌ • Press the <ENTER> key. Ｃ １．００Ｅ－１８ｍｏｌ SCROLL * The calculation formula for finding the number of cells is ＥＮＴＥＲ shown in Chapter 6, "Relation between Each Unit"． MAIN MENU Ⓒ Kikkoman Corp. (04—1)

  • Page 26: Atp/Cell Coefficient Input [Input Atp/Cell]

    MAIN MENU. MAIN MENU Input range 1.00E-20 mol to 1.00E-12 mol * If the value you have entered is outside the input range, the cursor moves back to that position, so re-enter the correct value. Ⓒ Kikkoman Corp. (04—1)

  • Page 27: Pg" Conversion Coefficient Input [Input Coef.(Pg)]

    MAIN MENU. ＹＥＳ＝ＥＮＴＥＲ ＮＯ＝ＦＵＮＣＴＩＯＮ ＥＮＴＥＲ MAIN MENU Input range 1.00E+05 to 1.00E+20 * If the value you have entered is outside the input range, the cursor moves back to that position, so re-enter the correct value Ⓒ Kikkoman Corp. (04—1)

  • Page 28: Calibration Curve Input [Input Cal.curve]

    ＮＯ＝ＦＵＮＣＴＩＯＮ ＥＮＴＥＲ MAIN MENU Input range 1.00E-17 to 1.00E-10 -1.00E-08M to -0.00E-00M * If the value you have entered is outside the input range, the cursor moves back to that position, so re-enter the correct value. Ⓒ Kikkoman Corp. (04—1)

  • Page 29: Measurement Time Setting [Measuring Time]

    * If the value you have entered is outside the input range, the cursor moves back to that position, so re-enter the correct value. * About measurement data display Values displayed in RLU (relative light unit) at the end of measurement are the average of the count rate measured during the measurement time. Ⓒ Kikkoman Corp. (04—1)

  • Page 30: Monitor Mode [Monitor Mode]

    ARE YOU SURE？ * Measurement data display when MONITOR mode is ON: When MONITOR mode is ON, the accumulated count ＹＥＳ＝ＥＮＴＥＲ value within the GATE TIME is displayed. This is not the ＮＯ＝ＦＵＮＣＴＩＯＮ count rate. ＥＮＴＥＲ MAIN MENU Ⓒ Kikkoman Corp. (04—1)

  • Page 31: Relation Between Each Unit

    The relation between RLU (relative light unit) and ATP concentration is as follows: Slope "a" is obtained by measuring standard ATP solution. "b" is the "blank" light level represented in ATP concentration. curve Calibration ＡＴＰ concentration (M) Slope Relative light unit (RLU) Ⓒ Kikkoman Corp. (04—1)

  • Page 32: Atp Amount (Pg)

    When the sample liquid amount is 100 microliters, the default value can be used as is. Number of cells (CELL) Conversion Coefficient ATP concentration (M) Number of cells (CELL) ATP concentration (M) Number of cells (per milliliter) = × 10 ATP (mol) / CELL Ⓒ Kikkoman Corp. (04—1)

  • Page 33: Computer Interface

    Computer Interface The LUMITESTER C-110 is supplied with computer software "LUMITESTER C-110 Utility Software". This software allows you to easily create and register a calibration curve and to perform data transfer. See "Utility Software Operation Manual" for detailed information. This section explains how to transfer data to the computer without using the utility software.

  • Page 34: Maintenance

    • Check with your dealer to make sure you buy the correct battery pack. CAUTION! Always use the specified battery pack. If other types of battery packs are used, this may cause malfunction or may damage the unit. Ⓒ Kikkoman Corp. (04—1)

  • Page 35: Specifications

    Software is supplied for data transfer and calibration curve Software creation/registration. Power Rechargeable battery or AC adapter Dimensions 185W x 110D x 75H mm (excluding projecting parts) Weight Approx. 700g Storage -10 to +50°C Temperature Operating +5 to +40°C Humidity No condensation Ⓒ Kikkoman Corp. (04—1)

  • Page 36: Dimensions

    10. Dimensions Dimensions are in millimeters Ⓒ Kikkoman Corp. (04—1)

  • Page 37: After Sales Service

    1. The product was purchased long time ago. 2. The product uses maintenance parts which are not in current production. 3. The product was severely damaged. 4. The product was modified. 5. Problems cannot be observed by us. 6. Other similar cases Ⓒ Kikkoman Corp. (04—1)

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