Sakura Tissue-Tek AutoTEC Operating Manual

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Summary of Contents for Sakura Tissue-Tek AutoTEC

Page 4: Table Of Contents
TABLE OF CONTENTS Section Page INTRODUCTION Safety Precautions ..............................1.1 General Description ............................1.1 Location of Major Systems and Components ..................1.1 Specifications ................................1.6 Safety Standards ..............................1.7 INSTALLATION General Information ............................2.1 Environmental Factors.............................2.1 Unpacking and Installation..........................2.1 Moving..................................2.2 CUSTOMIZATION OF SETTINGS General Information ............................3.1 Map of Interface Screens..........................3.5 Access Levels .................................3.6 Configuring System Settings .........................3.7...
Page 5 TABLE OF CONTENTS Section Page TROUBLESHOOTING Error Recovery ..............................6.1 Error Codes and Messages ..........................6.2 Error Recovery Procedure ..........................6.7 SERVICE AND REPLACEMENT PARTS Service..................................7.1 Replacement Parts..............................7.1 APPENDIX A Glossary..................................A.1 APPENDIX B Index ..................................B.1 APPENDIX C Grossing Procedures for Paraform Sectionable Cassettes ............C.1 ®...
Page 6: Introduction
Components of laboratory procedures and operations  Provide robotic precision and consistency, minimize The Tissue-Tek AutoTEC instrument is comprised of the the bottom paraffin layer, and eliminate excess following major systems and components (Figures 1-A paraffin around the cassette border through 1-C).
Page 7 INTRODUCTION Figure 1-A: Front View and Left Panel Figure 1-B: Rear View and Right Panel Location Component Touch screen monitor Main door, for access to input door, output doors and paraffin drip tray Front door, equipped with safety interlocks. Allowing operator easy access to base molds and air filter Paraform recovery chute door, for retrieval of rejected cassettes Paraffin reservoir lid;...
Page 8 Section 1 Input Door and Loading Area (Figure 1-D) Robotic System (Figure 1-E through 1-H)) Figure 1-E: Interior View of Robotic System Figure 1-D: Input Door As shown in Figure 1-E, the robotic system comprises two main modules, a gripper , and a stager , each with The input door is accessed by opening the main door (see...
Page 9 Please contact The Teflon coated base mold module comprises three Sakura, if the Pre-fill method is needed. channels of eight base molds each. The base molds in the When paraffin is dispensed, the base mold switches to its first channel, closest to the back of the system, are sized cooling mode.
Page 10 Section 1 Output Doors (Figure 1-I) Paraffin Reservoir (Figure 1-J) Figure 1-J: Paraffin Reservoir, Lid Open Figure 1-I: Output Doors The paraffin reservoir stores and heats up to 5.38 liters of paraffin. This is enough to embed up to 960 Paraform The four output doors are interlocked to prevent operator cassettes, or approximately eight hours of operation.
Page 11: Specifications
INTRODUCTION Control Panel (Figure 1-K) Specifications The Tissue-Tek AutoTEC operator interface is a touch  Models Covered by this Manual screen control panel that operates a Windows -based application program. Model Name/Description Tissue-Tek AutoTEC Automated Tissue Embedding ® ® 7000...
Page 12: Safety Standards
Section 1 Dimensions and Weight Height: 154 cm (60.6 in.) Width: 136 cm (53.7 in.) Depth: 76 cm (29.9 in.) Weight: 386 kg (850 lbs) Instrument Life Expectancy 7 years, based on eight hours of operation, five days a week, with a maximum of 960 cassettes per day; if the operating and instrument care instructions found in this manual are followed, and if preventative maintenance is performed at least once every year by a qualified service...
Page 13 INTRODUCTION Revised 5/19/2010...
Page 14: Installation
Tissue-Tek AutoTEC must be located and installed correctly to ensure proper operation and service. The Tissue-Tek AutoTEC is shipped in a large carton. Read this operating manual carefully before attempting to Inspect the carton for shipping damage. If there is visible operate the Tissue-Tek AutoTEC instrument.
Page 15: Moving
Once the main power switch has been turned off, open the reservoir access door to let heat out to speed the paraffin cooling process. CAUTION: Do not move the Tissue-Tek AutoTEC while paraffin is in a liquid form in the reservoir. To move the AutoTEC: 1.
Page 16: Customization Of Settings
Section 3 CUSTOMIZATION OF SETTINGS After a successful log in, display screens can be changed General Information by selecting the tab for each screen and buttons are enabled to allow the initiation of the embedding process. The four Tissue-Tek AutoTEC main tabbed screens ®...
Page 17 CUSTOMIZATION OF SETTINGS Button Descriptions INSTRUMENT ACCESS When displayed, it allows the user to pause the system operation, access the interior of the system by opening the STOP front or rear doors, and resume operation by touching OK When selected, it pauses the current instrument activities in an on-screen prompt.
Page 18 Section 3 UTILITIES Screen Utility screen is displayed, when the UTILITIES tab is SYSTEM SETUP selected (Figure 3-D). When selected, is used to set up alarm volume, alarm sound, end of process sound, current year, date and time, and institution name. SHUT DOWN When selected, is used to shut down the AutoTEC Software applications, Windows operating system PC, and...
Page 19 CUSTOMIZATION OF SETTINGS ERROR LOG Screen − Displays information on the cassettes ran, based on the following selected criteria: ERROR LOG screen is displayed, when the ERROR LOG  Date ranges tab is selected (Figure 3-E).  Today, This Week, Last Week, or this month.
Page 20: Map Of Interface Screens
Section 3 Map of Interface Screens Figure 3-H illustrates the general structure of the interface available for an administrator level clearance, showing screens accessible from the Monitor screen. Figure 3-H Revised 5/19/2010...
Page 21: Access Levels
This section provides the procedures to perform initial setup. Perform the following setup procedures before routinely using the Tissue-Tek AutoTEC system. In addition, perform these procedures as needed to reset the clock, add or remove users, change automatic start-up and cool- down times, etc.
Page 22: Configuring System Settings
Section 3 Following a successful login, the Initial MONITOR screen Administrator level access allows access to all utility will appear (Figure 3-J). functions. Operator level access is restricted to the following functions, some being on a view only basis. These are: Full Access: ...
Page 23 CUSTOMIZATION OF SETTINGS NOTE: The times set in this screen will repeat weekly unless Days Off is specified. Ready/Cool Down Times NOTE: In Auto At least one ON TIME and one OFF TIME must be set. Operators can program automatic timers to initiate the four-hour warm up routine at specific times on specific NOTE: To control the Ready/Cool Down time manually, days so that the AutoTEC will be ready for the first run...
Page 24 Section 3 NOTE: If a Cool Down time was programmed for that day, this forced cool down will override that time for the current day only. The programmed time will remain active for future Shut Down days. Refer to page 3.8 about how to program cool down Touch the Shut Down icon from the Utilities Selection times.
Page 25 CUSTOMIZATION OF SETTINGS Touch the MONITOR tab to display the MONITOR screen. COOL DOWN will be displayed at the upper-left corner of the screen, as seen in Figure 3-Q. Figure 3-R: Software Version screen Figure 3-Q: Run screen, Cool Down Mode Clean Gripper Arms A count-down timer in the upper-right corner will indicate the remaining time until the automatic start-up sequence is...
Page 26 Section 3 To set system parameters: The RESTORE DEFAULTS restores all settings to their defaults. System Setup Set-up parameters, defaults, and functions are as follows: The System Setup screen (Figure 3-S) enables the administrator to set a variety of system parameters. Parameter Default Function...
Page 27 CUSTOMIZATION OF SETTINGS Once a language selection is made from the drop down Audible Alarm Setup list, the System Setup screen will be displayed in the As shown in Figure 3-X, System Setup screen, there are selected language immediately (Figure 3-V). However, three alarm attributes that can be modified.
Page 28 Section 3 DATE and TIME Setup INSTITUTION Setup For current DATE setting, select the DATE down arrow to Select EDIT to bring up CHANGE INSTITUTION NAME activate the calendar screen. Select the correct date keyboard (Figure 3-AA). Use BACKSPACE to clear (Figure 3-Y).
Page 29 CUSTOMIZATION OF SETTINGS NOTE: Passwords and user names can only have a maximum of 8 characters. User Accounts NOTE: Administrators may change or delete passwords and user names but passwords are never displayed. If an From the Utilities screen (Figure 3-BB), touch the USER operator forgets their password, it can be reset.
Page 30 Section 3 Error Log  By Error ID. Select the Error ID pull down arrow to display the pull down menu. Scroll to the By selecting the ERROR LOG tab, the ERROR LOG selection (Figure 3-GG). screen is displayed (Figure 3-EE). The error log displays chronological information about system errors and events that occur, including a number and description, run number, date and time of the error or...
Page 31 CUSTOMIZATION OF SETTINGS  Output files can be saved in any one of the three formats, he ALL button will display a listing of ALL HTML, PDF, or EXCEL to help in organizing the collected errors that are logged in the instrument, from the data.
Page 32 Section 3 NOTE: When using date range; after the date selections, select the Go button first to update the display. The Summary displays the days selected and the total numbers of cycles, magazines, cassettes, types of cassettes, etc (Figures 3-LL and 3-MM). Figure 3-NN: One day view By selecting a magazine from the summary screen, details of all the cassettes in this magazine are displayed...
Page 33 CUSTOMIZATION OF SETTINGS Displayed columns can be toggled between, expanded, or By selecting the File Output button; the selected run compressed to fit the LCD display area, by selecting the history can be transferred to a flash drive (Figure 3-RR). Expand All Columns button (Figure 3-PP).
Page 34: Operation
Monitor screen will display (Figure 4-A). Figure 4-C Figure 4-A: System Start Up screen Typical Operating Sequence A typical operating sequence for the Tissue-Tek AutoTEC 2. Select the LOG IN button to access the ENTER is as follows: PASSWORD screen (Figure 4-B). ...
Page 35: Routine Operation
The warm up period can be programmed to start automatically at a set time. Following warm up, the Tissue-Tek AutoTEC is in Operating mode, ready to embed. A valid user must be logged in to start embedding.
Page 36 Section 4 To load magazines into the input door, follow these Load Magazines instructions: Usually, the Paraform Cassettes are assembled and 1. Properly assembled and placed frame/cassettes inserted into the magazines at the grossing station. inside the magazine are necessary for uninterrupted Magazines are standard- or Tissue-Tek Xpress Rapid...
Page 37 OPERATION 5. Hold the magazine upright against paper towels or inserted magazines will likely be ready for embedding other absorbent material to allow excess paraffin to for maximum efficiency. drain off the magazine before placing it in the The Warming Cassettes screen (Figure 4-I) specifically AutoTEC.
Page 38 Section 4 Run Screen Layout Input Magazines Color Code Information The Run Screen layout is consistent through all phases of system embedding. As shown in figure 4-J, the four input Magazine is in the15-minute warm up period. magazines are shown on the left, while the four output Magazine warm up period is complete.
Page 39 OPERATION Embedding Cassettes Once loaded with magazines, the AutoTEC will start automatically and embedding will begin after the system checks and 15-minute magazine warm up period are complete. During system operation it is possible to continually replace empty magazines with loaded magazines and full output doors with empty ones.
Page 40 Section 4 To remove one or more doors: Cassette Recovery Chute 1. Push the small red button .1. located above the In certain instances, an improperly assembled Paraform upper-right corner of the output door to release the frame and cassette will be placed in the cassette recovery latch (Figure 4-M).
Page 41 OPERATION Revised 5/19/2010...
Page 42: Care Of The Instrument
Do not use solvents of any kind on the cabinet or control Proper maintenance of the AutoTEC is essential to panel; in particular, do not use acetone on the Sakura logo maintain the system in operative condition. This section or any other labeled or printed surface.
Page 43: Paraffin Reservoir And Filter Cleaning
CARE OF THE INSTRUMENT Output Grid Sensor Cover Glass Cleaning Filter Cleaning The output grid sensor is located along the lower channel CAUTION: When using solvents, refer to their Material of the output doors and is protected by a cover glass (See Safety Data Sheets and always observe precautions Figure 5-B).
Page 44 Section 5 3. Turn the filter 90° so it can be lifted out of the 7. Gently pull the parasitic filter straight out from the reservoir. Rotate by holding the top of the filter until center (Figure 5-F). tabs are turned away from the locked down positions (Figure 5-D).
Page 45: Magazine Cleaning
Output Door Inspections and Cleaning Output doors must be periodically inspected for deformed spring clips (Figure 5-H). Do not use a door with any deformed springs; contact Sakura to have it replaced. Figure 5-I Figure 5-H: Examples of deformed Output Door springs...
Page 46: Gripper Weekly Maintenance
Section 5 Gripper Weekly Maintenance The gripper arms should be cleaned weekly. To prevent paraffin accumulation, two possibilities are recommended. 1. First run on Mondays – Each Monday after the operator loads magazines and closes the input door, on the first cycle of the day, the following message will be displayed (Figure 5-K): Figure 5-L Open the front door to clean the gripper arms (Figure 5-M)
Page 47 CARE OF THE INSTRUMENT Gripper Arms Cleaning Procedure The instrument will prompt the operator to clean the gripper arms once a week (Figure 5-N). Gripper Figure 5-O Arms Figure 5-P Figure 5-N After cleaning the gripper, close the front door to allow the instrument to resume operation.
Page 48 Section 5 2. Gripper Arms Parallel Check fails: If Gripper Open the front door to clean the gripper arms (Figure 5-S) Parallel Check fails, the instrument prompts the and follow Gripper Arms Cleaning Procedure located on operator to clean the gripper arms (Figure 5-Q). the previous page.
Page 49 CARE OF THE INSTRUMENT Revised 5/19/2010...
Page 50: Troubleshooting
 Document error codes for reference when contacting up window on the control panel but also an audible alarm Sakura Finetek for service sounds. NOTE: Internal events are recorded in the Error Log but are not considered errors which require corrective action.
Page 51: Error Codes And Messages
TROUBLESHOOTING ERROR CODES AND MESSAGES Error Codes and Messages Title Error Result, or Message Code Action Required Error Type Error Name Unexpected Frame Problem Error Log UNEXPECTED FRAME PROBLEM User can interrupt the stall recovery process by touching ABORT button; this allows user to access the instrument and AXIS STALL IN THE U AXIS move any axis manually, if required.
Page 52 The drip tray heater -<temperature> is out of acceptable operating range <Min.-Max.>, instrument will continue Perform action/s HEATER TEMPERATURE PROBLEM embedding as normal. Contact Sakura Service requested. Department. Please replace Output Door #<1–4>, then touch OK to OUTPUT DOOR #<1–4> IS Touch OK resume instrument operation.
Page 53 TROUBLESHOOTING The Frame Heater temperature [##] is out of acceptable range [Min: ##, Max:##] HEATER FRAME HEATER OUT OF Please empty all input & output doors and contact Sakura Perform action/s TEMPERATURE RANGE Service Department for further instructions. requested PROBLEM Please touch RESUME to restart the application or ABORT to shutdown the instrument.
Page 54 Unable to move Frame Heater. Both the limit switches are BOTH THE LIMIT triggered. Instrument shuts SWITCHES OF FRAME FRAME HEATER Please empty all input & output doors and contact Sakura down if [OK] is MOTOR PROBLEM HEATER MOTOR ARE Service Department for further instructions. touched TRIGGERED Please touch OK to shut down the instrument.
Page 55 Contact Sakura POWER – ON THE INSTRUMENT PLEASE DO NOT TURN OFF THE MACHINE. Contact Service Department PROBLEM Sakura Service Department for further instructions. AMBIENT TEMPERATURE Ambient Temperature exceeds operating requirements. AMBIENT EXCEEDS ACCEPTABLE Embedding Stopped, until ambient temperature lowered.
Page 56: Error Recovery Procedure
Section 6 Error Code Notes If error messages continue to occur after implementing the error recovery procedure, contact Sakura Finetek for For errors 13 through 22, the error number will vary further assistance. See Section 7, Service and depending on the subsystem affected.
Page 57 TROUBLESHOOTING For rear access, use a 5/32” (4mm) Allen wrench to turn NOTE: Only users with a service-level log in can re-enable the two latches, and swing the doors open. (This tool is a base mold once it is disabled. included in the AutoTEC accessory kit).
Page 58 Section 6 If the operator continues, another message will prompt the cannot be resolved, do not use this magazine position and operator to remove any block that may be present in the call for service. disabled base mold when the cycle is complete (Figure 6-G). Cassette Lid Open Figure 6-G If base mold #8 is disabled, then an additional button...
Page 59 TROUBLESHOOTING 6.10 Revised 5/19/2010...
Page 60: Service And Replacement Parts
Output Doors (product code 7008): 2/pkg Where to Call for Service If located within the United States, contact the Technical Support Department of Sakura Finetek USA, Inc. by calling toll free: 1-800-725-8723, Menu Option “2” In countries other than the United States, contact the nearest authorized Sakura Finetek instrument distributor or representative for service information and technical assistance.
Page 61 SERVICE AND REPLACEMENT PARTS Consumables  Tissue-Tek Paraform Processing/Embedding ® ® Medium, Formula 3 (product code 7052): 8x1kg  Tissue-Tek Paraffin Trough (product code 7011) 12 ® paper troughs/cs  Tissue-Tek Paraform Low Profile Disposable ® ® Blades (product code 7089): 10 Dispensers x 50 blades/cs ...
Page 62 Order Management Sakura Finetek U.S.A., Inc. 1750 W. 214th Street Torrance, CA 90501 or by calling toll free: 1-800-725-8723 Outside the United States, contact the nearest authorized Sakura Finetek instrument distributor.  Tissue-Tek Accu-Edge Grossing Fork Cleaning ® ® Brushes (product code 4814): 10/pkg ...
Page 63 SERVICE AND REPLACEMENT PARTS Revised 5/19/2010...
Page 64: Appendix A Glossary
Appendix A GLOSSARY AutoTEC A reliable, high-quality automated precision instrument that embeds diagnostic tissue ® specimens continuously, forming sectionable paraffin blocks. Base Molds Molds contained in the base mold assembly that are used to hold, heat, embed, and cool standard, biopsy, or orientation specimens in cassettes. Cassettes Paraform components that are sectionable and hold tissue specimens during the embedding process.
Page 65 GLOSSARY Stager Assembly Positions the paraffin dispensing mechanism over the cassette in the base mold, presses down on the cassette to ensure it is correctly positioned in its frame, and dispenses an even flow of molten paraffin to the correct depth. Ready Mode Condition in which the system is warmed up, but is not embedding.
Page 66: Index
Appendix B INDEX Error code notes ..............6.4 Error codes and messages ........... 6.7 Access Levels ................3.6 Procedure................6.7 Accessories ................. 7.1 Exporting Data ................3.14 Accessories kit ................2.1 G H I Ancillary products................ 7.2 AutoTEC Glossary..................A.1 Description ................1.1 Humidity Conditions..............
Page 67 INDEX Paraffin, adding ................4.7 Unpacking and Handling ............2.1 Paraform recovery chute ............1.4 User icons Passwords, managing ............3.6, 3.14 Administrator ................3.6 Programming Screens Operator ................. 3.6 Audible alarm setup............. 3.12 Date and Time Setup............3.13 Days Off Selection ..............3.8 Enter passwords ..............
Page 68 Revised 5/19/2010...
Page 69 Revised 5/19/2010...
Page 70 Revised 08/01/2005...