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Quick Start Guide
SNAP i.d.
2.0 IHC (Immunohistochemistry) System
®
For research use only
Before using the SNAP i.d.® 2.0 IHC System, please read the full-length user guide available at www.millipore.com/ihc.
Component
Assembly Fixture
Accepts 25 × 75 × 1 mm slide
(slides not provided)
Slide Holder
Injection/recovery port accepts
1 mL pipette tip
IHC Frame
Holds up to 12 slide holders
Incubation Cover
SNAP i.d.® 2.0 IHC System
Accommodates one or two
IHC frames
Includes tubing assembly kit
Features
Disassembly slots
Injection/recovery port
Injection/recovery
port fluid channel
Silicone gasket
Vacuum flush port
Vacuum ports (12)
Center well for
assembly fixture
storage
Tissue section target (guides tissue
section size and placement)
Volume markings (for estimating
solution volume required; each
line represents ~ 150 µL)
Wash reservoir
(6 mL maximum volume)
Tissue section chamber
(600 µL maximum volume)
Slide compression clips (4)
Port plugs (to maintain vacuum
when port is not being used)
Port plug storage holes
Stacking recesses
(to enable IHC frame stacking off-line)
Task tracker (for keeping track of steps)
Vacuum control knob
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Table of Contents
Summary of Contents for M SNAP i.d. 2.0 IHC
- Page 1 Quick Start Guide SNAP i.d. 2.0 IHC (Immunohistochemistry) System ® For research use only Before using the SNAP i.d.® 2.0 IHC System, please read the full-length user guide available at www.millipore.com/ihc. Component Features Tissue section target (guides tissue Assembly Fixture section size and placement) Accepts 25 ×...
- Page 2 Setup IHC Frame Setup The IHC frame can be set up either on or off the General Guidelines SNAP i.d.® 2.0 system. ● Do not use the SNAP i.d.® 2.0 IHC system for 1. Place assembled slide holders into any vacuum ports deparaffinization, rehydration, dehydration, or mounting on the frame, with slide labels oriented towards the steps.
- Page 3 Washing (optional) Reporter (tertiary, if required) 1. Inject up to 600 µL of reporter through the injection/ 1. If washing is required before blocking, fill the wash reservoir of the slide holder with up to 6 mL of desired recovery port, dispensing slowly to avoid bubbles, and wash solution.
- Page 4 290 Concord Rd., Billerica, MA 01821 The applicable warranty for the products listed in this The M logo, Millipore, and SNAP i.d. are registered trademarks of Merck KGaA, Darmstadt, Germany. publication may be found at www.millipore.com/terms © 2015 EMD Millipore Corporation. Billerica, MA, U.S.A. All rights reserved.
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