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Accuris MR9610 Operation Manual

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Summary of Contents for Accuris MR9610

  • Page 2 Operation Manual Version 2.0 MR9610, SmartReader™ UV-Vis Microplate Reader This instrument is for research use only...

  • Page 3: Foreword

    Foreword Thank you for purchasing our MR9610, SmartReader™ UV-Vis Microplate Reader. This user manual describes how the instrument works and includes an operation guide, please read it carefully before operation and keep for future reference. Initial check Please check the instruments as well as all accessories with the packing list when first received and unpackaged.

  • Page 4: Safety Tips

    Accuris. Performing any unauthorized service repairs will result in loss of warranty coverage. Contact Accuris Instruments or an authorized distributor or agent in the event of any service issues. Before powering on, confirm that the voltage used meets the electrical requirements of the instrument as stated on the rating plate.
  • Page 5 The Instrument should be installed in an environment of standard room temperature, low dust, low humidity and away from direct sunlight. Also make sure there is adequate ventilation, no corrosive gas or strong magnetic field. Always power off the instrument when you are finished using it. Unplug the power cord and cover the instrument with a cloth or plastic sheet to prevent excessing dust from entering the housing.

  • Page 6: Table Of Contents

    Contents Foreword ..............................III Initial check ............................III Safety Warning and Guidelines ......................III Important information for safe use ..................III Safety Tips ..........................IV Chapter 1 Introduction ........................1 Chapter 2 Features ..........................2 Chapter 3 Instrument Overview ....................4 Chapter 4 Installation ..........................

  • Page 7: Chapter 1 Introduction

    MR9610 Microplate Reader Operation Manual Chapter 1 Brief Introduction Chapter 1 Introduction The SmartReader™ UV-Vis is an automated microplate reader for ELISA assays. Through absorbance readings, it can determine the concentration, as well as positive or negative detection of antibody and antigen in samples when used with ELISA kits and solutions.

  • Page 8: Chapter 2 Features

    MR9610 Microplate Reader Operation Manual Chapter 2 Features Chapter 2 Features Working conditions： Ambient temperature: 10C~40C Relative humidity: 30%~80% (Non-condensing environment) Power: AC 100-240V 50-60Hz 2A Parameters: Model MR9610 Parameter Light source Xenon flash lamp >10 flashes Wavelength 200～1000nm Bandwidth <...
  • Page 9 MR9610 Microplate Reader Operation Manual Chapter 2 Features 1 B-type USB port for PC connection Connections 1 Ethernet port 2 A-type of USB ports for mouse and printer connections Included power adapter: DC24V 6.67A 160W, Power requirements AC 100V – 240V Dimension (W×D×H)

  • Page 10: Chapter 3 Instrument Overview

    MR9610 Microplate Reader Operation Manual Chapter 3 Instrument Structure Chapter 3 Instrument Overview Structure Rear cover Touch screen Housing Front door Extension module Plate holder External Buttons B-type USB Port USB Ports LAN Port Air outlet DC Power Inlet Power Switch ─...

  • Page 11: Chapter 4 Installation

    Chapter 4 Installation Chapter 4 Installation 1. Initial check Each MR9610 is thoroughly tested before shipping, but please check it again when you receive the instrument and contact your local distributor or Accuris Instruments if: • The outer package is damaged •...
  • Page 12 MR9610 Microplate Reader Operation Manual Chapter 4 Installation Note: One cord coming from the power adapter will fit to the input jack on the back of the instrument. Make sure to connect it according to the above picture. The other cord will connect to a standard electrical outlet (100VAC to 230VAC) ③...

  • Page 13: Chapter 5 Operation Guide

    MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Chapter 5 Operation Guide 1. Initial self-check This chapter explains a standard protocol set up and operation, beginning with the automatic self-check after power on. The following screen image will appear during the...

  • Page 14: System Settings

    Note: Please keep the Admin password recorded and in a safe place, and contact Accuris Instruments or your distributor if the Admin password is lost or forgotten. After logging in, the Home Interface screen is displayed (Fig 4.) Fig 4 The “Admin”...

  • Page 15: Protocol Management

    MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 5 Note: 1. The instrument must be manually powered off and back on after the date and time settings are changed. 2. The Maintenance function is accessible only by authorized service representatives.
  • Page 16 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Navigation bar Main display area The optional bar Setting Type Selection Fig 6 Protocol name can be modified by clicking it directly. The default name of a new protocol is the system date and time, and it can be modified manually.
  • Page 17 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Protocol list in “edit” state Protocol list, standard state Fig 7 When the protocol list is in the standard state, users can perform the below operations: ⚫ Search: Enter a keyword to search the list.

  • Page 18: Reading A Microplate

    MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide 4. Reading a Microplate After a protocol name is created, the next step is to set the parameters according to the experiment requirements. 4.1 Plate layout settings The interface will automatically display the plate in a 96 well plate layout when creating a new protocol.
  • Page 19 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Standard: Used for creating the standard curve. After clicking “Standard”, the option bar will allow setting replicates and also calculations for quickly setting the standard concentrations, see Fig 9. ⚫ Replicates: Use to set the same standard for multiple wells.
  • Page 20 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 9 Note: The plate layout can be set up before or after the plate is read. For example, users can set all wells as “unknown”, read the absorbance values, then set up the layout of the plate.
  • Page 21 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide ⚫ Incubator: The plate chamber can be set to a specified temperature, and the actual temperature is displayed. ⚫ Wavelength: shows wavelength parameters of the current protocol. ⚫ Shake: This function can be set to on or off.
  • Page 22 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Table 2 Endpoint Kinetic Spectrum ┗ Selected Parameters ┗ Selected Parameters ┗ Selected Parameters ┗ Wavelength ┗ Wavelength ┗ Wavelength ┗Mode ┗Mode ┗Mode ┗Fast ┗Fast ┗Fast ┗Precision ┗Precision ┗Precision ┗ Wavelength ┗...
  • Page 23 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide 4.3 Reading a Plate Click “ ” on the screen or press “Plate in/out” on the front of the enclosure to open the plate drawer, load an ELISA plate on the plate holder, paying attention to the orientation of the plate so the A1 well is in the correct location.

  • Page 24: Result Processing

    MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 12 5. Result processing The screen will stay in the “Run” interface after detecting samples and will display the original absorbance values measured. To view the analyzed results, switch to the “Result”...
  • Page 25 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide 5.1 Endpoint result As Fig 13 shows, data processing options for an endpoint result include: “Raw Data”, “Blank Subtraction”, “Basic Calculation”, “Standard Curve”, “Classification” and “Quality Control”. ⚫ Raw data: displays absorbance values of each well. If the plate was read using multiple wavelength, the user can switch to different wavelengths by pressing the button “...
  • Page 26 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide the “Parameters” window on the right of the interface. Below are the 8 curve fitting types available: ➢ Linear fitting (Minimum Standards required: 2) ➢ Four Parameters Logistic (4PL) (Minimum Standards required: 4) ➢...
  • Page 27 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 15 Note: The Protocol layout interface must be set with at least one positive or negative well, or the “Classification” button will not be available. See Fig. 15 ⚫ Quality Control: Click “Quality Control” button, (see Fig 16), the main display area...
  • Page 28 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide 5.2 Kinetics After a kinetic type assay, the results screen will display small graphs inside the squares instead of absorbance values (see Fig 17). Fig 17 To see the detailed absorbance results for a specific well, select the target well, then click the “Graph”...
  • Page 29 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide please see Section 5.1. Clicking the “Kinetic Analysis” button, the window on the right side will offer different calculation options, (see Fig 19). The calculations available include: ➢ Average/SD/CV% ➢ Integral ➢...
  • Page 30 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Fig 20 5.3 Spectral Analysis click “Graph” button to enlarge the spectral See Fig 20 (above), select the target wells curve, the absorbance of each wavelength shown on the curve. Users can click the “Back”...
  • Page 31 MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide ➢ Select Single Wavelength ：read the measurement value of a single wavelength. Fig 21a Fig 21b Description of each button on the upper right corner of the main display area: Print the current content of the main display area to a...

  • Page 32: Report Exporting

    MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Choose a well to view the kinetic curve, the “Graph” button can enlarge the curve, see Fig 17, “Back” is to return to the main interface. Switch from plate layout view to a data list display, click the “Plate”...

  • Page 33: Power Off

    MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide Choose the content to export in “Output Content” on the right side, “√” will appear next to the selected content, then click “Output” to export data to a flash-drive. “Print”: The print function can only be used to print the instrument’s basic information, including instrument serial number, software version etc.

  • Page 34: Chapter 6 Maintenance, Storage, Transportation

    ⚫ The SmartReader Instrument is calibrated before it leaves the factory. There are no user-serviceable parts inside, so please do not attempt to open the housing. If any defects or operation problems arise, please contact your distributor or Accuris Instruments at 908-769-5555 or info@accuris-usa.com.

  • Page 35: Chapter 7 Trouble Shooting

    MR9610 Microplate Reader Operation Manual Chapter 7 Troubleshooting Chapter 7 Trouble shooting Trouble description Possible reason Solution a. Check that there is power to the instrument The Microplate Reader b. Make sure that all power cords Power supply failure cannot be started are connected properly c.
  • Page 36 MR9610 Microplate Reader Operation Manual Chapter 7 Troubleshooting Check that the plate is properly seated, if liquid has spilled out and whether the front door is working Test results are Light path failure correctly, then re-start the unstable instrument. If problem still occurs,...

  • Page 37: Chapter 8 Accessories

    MR9610 Microplate Reader Operation Manual Chapter 8 Accessories Chapter 8 Accessories Item Type Unit Remarks Power cord AC/DC Power Adapter 24V 160W Mouse Logitech Performance test statement Operation Manual Packing List Flash Drive Certification...
  • Page 38 Memo...

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