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EpiShear™ Cooled Sonication Platform
Catalog Nos. 53080 (1.5 ml), 53081 (15 ml) & 53082 (50 ml)
www.activemotif.com
(version C1)
Active Motif North America
1914 Palomar Oaks Way, Suite 150
Carlsbad, California 92008, USA
Toll free:
877 222 9543
Telephone:
760 431 1263
Fax:
760 431 1351
Active Motif Europe
Avenue Reine Astrid, 92
B-1330 La Hulpe, Belgium
UK Free Phone: 0800 169 31 47
France Free Phone:
Germany Free Phone:
Telephone:
Fax:
Active Motif Japan
Azuma Bldg, 7th Floor
2-21 Ageba-Cho, Shinjuku-Ku
Tokyo, 162-0824, Japan
Telephone:
+81 3 5225 3638
Fax:
+81 3 5261 8733
Copyright 2014 Active Motif, Inc.
0800 90 99 79
0800 181 99 10
+32 (0)2 653 0001
+32 (0)2 653 0050
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Summary of Contents for Active Motif EpiShear
- Page 1 EpiShear™ Cooled Sonication Platform (version C1) Catalog Nos. 53080 (1.5 ml), 53081 (15 ml) & 53082 (50 ml) Active Motif North America 1914 Palomar Oaks Way, Suite 150 Carlsbad, California 92008, USA Toll free: 877 222 9543 Telephone: 760 431 1263...
- Page 2 Information in this manual is subject to change without notice and does not constitute a commit- ment on the part of Active Motif, Inc. It is supplied on an “as is” basis without any warranty of any kind, either explicit or implied. Information may be changed or updated in this manual at any time.
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Page 3: Table Of Contents
Parts Diagram............... .3 EpiShear Cooled Sonication Platform A. -
Page 4: Overview
Overview Active Motif’ s EpiShear™ Cooled Sonication Platform was designed to improve sample-to-sample reproducibility when using a probe sonicator to shear chromatin or DNA by enabling the tip of the probe to be positioned at the same depth in the sample each sonication. The platform has been... -
Page 5: Included Components
Included Components The Cooled Sonication Platform is supplied with the following components. • Sound enclosure with measurements of 28” H (with chimney) x 14.25” W x 12” D (711 mm x 362 mm x 305 mm) • Probe chimney • Sonication platform •... -
Page 6: Parts Diagram
Parts Diagram Converter Size Continuous Converter/Probe Adapter Force Spring Bracket Stop Collar Latch Probe Tube Counter Cooler Hand Crank Platform Thumbscrew The continuous force spring helps support the weight of the platform and enables it to move up and down the rod smoothly, either using the latch or the hand crank. The stop collar is used to set the highest point on the rod that the platform can be set. -
Page 7: Epishear Cooled Sonication Platform
EpiShear™ Cooled Sonication Platform During shipment of the EpiShear Cooled Sonication platform, the probe chimney, tube cooler, alignment tool and hex wrenches are placed inside of the sound enclosure. Carefully remove each item and perform a visual inspection to detect any damage that may have occurred during ship- ment. -
Page 8: Placement Of The Converter/Probe
B. Placement of the Converter/Probe To ensure sample-to-sample consistency, the platform has a bracket secured near the top of the rod that holds the sonicator converter/probe. This bracket enables you to fix the placement of the probe tip. Depending on the probe to be used, you may need to adjust where the converter bracket is located vertically on the rod. -
Page 9: Calibration Of The Platform Counter
C. Calibration of the Platform Counter The counter on the platform needs to be set to an appropriate “zero” point to enable you to return the platform to the same height consistently once you have determined the appropriate height for your purposes. - Page 10 The crank is then turned to slowly raise the stand up the rod. When the probe begins to enter the Alignment Tool, you may need to adjust the position of the stand so that the probe is centered in the tool. Simply loosen the two thumb screws underneath the stand, use one hand to move the stand forward/backward and left/right until the probe is centered, then re-tighten the thumbscrews.
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Page 11: Using The Cooled Sonication Platform
Using the Cooled Sonication Platform Benchtop Tube Coolers The Benchtop Tube Coolers can keep the temperature of a sample at or below 4°C for up to 2 hours of sonication if the cooler has been placed in a -20°C freezer overnight. If a large amount of time will pass between each sonication, return the cooler be to a -20°C freezer between samples. -
Page 12: Appendix
Appendix Section A. Attaching the Alternate Spring Bracket The alternate spring bracket was provided in case you need to remove the converter/probe bracket from the top of the rod, which you would need to do if your converter/probe is too large to be held by the bracket. - Page 13 Set the EpiShear Cooled Sonication Platform upright and proceed to Calibration of the Platform Counter on page 6.
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Page 14: Section B. Related Products
Section B. Related Products Sonication Format Catalog No . EpiShear™ Probe Sonicator 110V 53051 230V 53052 EpiShear™ 5/64” (2 mm) Sonicator Probe 1 probe 53056 EpiShear™ 1/8” (3.2 mm) Sonicator Probe 1 probe 53053 EpiShear™ 1/4” (6.4 mm) Sonicator Probe 1 probe 53057 EpiShear™... -
Page 15: Technical Services
Technical Services If you need assistance at any time, please call Active Motif Technical Service at one of the numbers listed below. Active Motif North America 1914 Palomar Oaks Way, Suite 150 Carlsbad, CA 92008 Toll Free: 877 222 9543...
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