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Northwest Leica DM6B Quick Start Manual

Guide for LAS X Navigator (Tile scanning)

Initial set-up

~ Place slide on stage 'coverslip up'.

~ For transmitted light imaging: reference Quick Guide for Bright Field (color) imaging.

~ For fluorescent light imaging: reference Quick Guide for Fluorescent imaging.

~ Focus on sample. Find cell/region of interest.

~ Set imaging settings as required by experiment.

Press the Navigator button located near the top of the Open

Projects/Acquisition panel.

~ In the new window find access to the standard LAS X settings on the left side (A-G). Adjust settings at

any time if required while staying in the Navigator mode.

~ Press "Carrier (G) > Well Plate > 4 slide" to view screen as 4 slide stage.

~ Using mouse wheel, zoom in/zoom out to the field of view of the interest.

~ Double clicking by left button of mouse will preview new areas of the slide. Also, Spiral button (H) can

be activated to preview sample by spiraling pattern. Stop it when needed.

~ Using one of Shape tools (I) draw border round the region(s) of interest (J). Each selection will be listed

in a Task List (K).

New window will open.

Leica DM6B Widefield

Quick Start Guide

Table of Contents

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Page 1 Leica DM6B Widefield Quick Start Guide Guide for LAS X Navigator (Tile scanning) Initial set-up ~ Place slide on stage ‘coverslip up’. ~ For transmitted light imaging: reference Quick Guide for Bright Field (color) imaging. ~ For fluorescent light imaging: reference Quick Guide for Fluorescent imaging.
Page 2 ~ To create even focus across selected region at image acquisition step activate Focus Points button (L) and place 3-6 focal points in different parts of each selected area. The number of focal points depends on the size of the selection. ~ Press “Live”, then open Focus Map (M), select the first point, adjust focus by focus knob and press “Set Z”.