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Related Manuals for Aquila Biolabs LIS
Summary of Contents for Aquila Biolabs LIS
- Page 1 LIS user guide A handbook for automated feeding in shake flask applications...
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Page 2: Table Of Contents
Set up an experiment ............. 20 Setting up the LIS Cartridge ..........22 Leakage Test ..................23 Prepare your LIS System ............24 Assemble the Feed System ........... 25 Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru... - Page 3 Add new Parameters ................ 37 Charging the LIS Drive ................38 Disinfection ..................... 38 Updating the LIS Drive Firmware............39 Troubleshooting Guide ................. 40 Contact ....................43 Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru...
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Page 4: Introduction And General Considerations
Welcome to the LIS user guide and congratulations for your decision of choosing the LIS system to feed your shake flasks. The idea of this user guide is to give you access to all information required to install, integrate and use the LIS within your laboratory everyday life. - Page 5 The latest manual is available on our homepage under www.aquila-biolabs.de/service/downloads. In case of any question that might arise during the work with LIS do not hesitate to contact us (Contact details on pp. 44). LIS user guide revisions: Revision 0 30.08.2017...
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Page 6: The Lis-System - An Overview
1 LIS System LIS stands for Liquid Injection System. It allows automated feeding for shake flasks. The LIS system consists of four different components, the LIS Drive, the LIS Cartridge, the LIS Coordinator (available as add-on) and the LIS software (available as add-on). - Page 7 The LIS Drive is the physical heart of the LIS-system, as it performs the communication and dispensing tasks. It can be used in a stand-alone mode, where the Drive is controlled via its display, or in a wireless mode. Here, several Drives are connected to the LIS Coordinator through a 2.4 Ghz wireless...
- Page 8 Figure 4 Profile generator of the LIS software Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru...
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Page 9: Technical Specifications
Charging Source USB 2.0 Port Radio Frequency 2.4 Ghz Range up to 30 m Pump max. 1 ml/min Flow rate dependent on feeding profile dependent on environment Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru... -
Page 10: Recommended Software Requirements (Lis Software)
Windows Update. It is recommended to use the “High performance” power plan in the Windows power options. You can also contact us to verify your chosen system settings. Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru... -
Page 11: Recommended Operating Conditions
(e.g. the Cell Growth Quantifier by aquila biolabs). Please carefully determine acceptable shaking speeds with your setup. Depending on shaking speed and diameter. Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru... -
Page 12: Warnings
Warnings Do not use the LIS system or any of its components in water bath shakers! This might result in electric shocks, which could damage your health, the LIS and any other electric device around. Be careful when mounting the LIS cartridge onto the shake flasks. - Page 13 German and international law and may lead to compensation claims. The LIS Drive contains a lithium ion battery. Take special care considering following aspects: Do not dispose the battery / the LIS Drive into normal waste.
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Page 14: Declarations And Certificates
The aquila biolabs GmbH, Arnold-Sommerfeld-Ring 2, 52499 Baesweiler, Germany, herewith declares under its sole responsibility that all devices and equipment being part of the LIS system and being manufactured by the aquila biolabs GmbH are in conformity with the Council Directives regarding Low Voltage Equipment Safety (2014/35/EU) ... -
Page 15: Weee Conformity
Electronic components may contain various hazardous substances that could possibly exhibit negative impacts on your health and the environment. In order to avoid those effects aquila biolabs encourages you to make use of the appropriate local take-back and recycling systems for disposing electrical and electronic equipment. -
Page 16: Fcc Compliance
Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense. Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru... -
Page 17: Installation, Quick Start And Handling
A Single press on the knob selects an entry or confirms an input • Pressing and holding the knob for more than 5 seconds triggers a forced shutdown of the LIS Drive (only use this function if problems occur) Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru,... -
Page 18: Main Menu Structure
• Status: Shows the experiment status if an experiment is running • Control: Start or stop an experiment, shutdown the LIS Drive Leakage Test: test the assembly for leakage Estimate: estimate volume in cartridge (beta-function) Prepare: start the pressure regulation... -
Page 19: Lis Drive Menu Structure
Do Crash Dispense (not for Test-Mode profile RecoveryCo Single) Battery (mV) Battery (mA) About figure 6 Menu structure of the LIS Drive Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru... -
Page 20: Running An Lis Experiment
Running an LIS Experiment Auto Offset the Pressure Before using the LIS Drive the pressure sensor needs to be blanked. The Drive has a function called “AutoOffset” to set the actual blank. Make sure no filter or cartridge is connected to the Drive ... - Page 21 Total Runtime: Total time of the experiment (initial delay + dispensing time) Confirm the feeding profile by clicking “Back” figure 7 different types of feeding profiles Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru...
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Page 22: Setting Up The Lis Cartridge
25ml and that you know how much volume is in the cartridge. Use the lid of the LIS cartridge to seal the cartridge air-tight (3). This step is very crucial, so please ensure that the lid sits tightly on the cartridge (you should feel a “snap-fit”) -
Page 23: Leakage Test
The LIS Cartridge is designed to fit 38 (± 0.3) mm straight neck Erlenmeyer shake flasks. Do not try to fit it on other types of flasks. -
Page 24: Prepare Your Lis System
If the leakage test is successful, you can go on Prepare your LIS System After programming the LIS Drive and filling the Cartridge, LIS is now ready to be started: • Choose “Control” in the main menu and then “Prepare”... -
Page 25: Assemble The Feed System
Avoid strong shocks as they could lead to unwanted drop release into the shake flask The LIS Cartridge is designed to fit 38 mm straight neck Erlenmeyer shake flasks. Some of these flasks have bigger or smaller outer diameter. Do not force the cartridge on these flasks as the glass could break. -
Page 26: Start The Experiment
Sampling If you want to take a sample from your culture during an experiment, you can use the “Sample” function of the LIS Drive. Sampling does not pause the time. All feeding steps during the sample period will not be performed but directly carried out after the sample mode is stopped. -
Page 27: Stop The Experiment
500 ml or greater. The Cartridge bottom has a male luer connector where you can attach needles (e.g. B. Braun Sterican needles). As the LIS Drive is controlling the pressure inside the LIS Cartridge to avoid unwanted drop release, the height of the water head is an important aspect. In order to work properly, the LIS Drive needs to be individually configured for each type of needle. -
Page 28: Lis Software And Coordinator
To start the LIS Software, open the Windows start menu and choose “LIS Software” or double click the shortcut on the desktop, if available. In case the LIS Software is configured to start with Windows automatically or if it is already running, you can also double-click the LIS-Software icon in the Windows notification area A browser window will open with the LIS user interface. -
Page 29: Exiting The Lis Software
Exiting the LIS Software If you want to exit the LIS Software, right click on the LIS icon in the notification area and select “Exit”. The LIS Software needs to run all the time while LIS experiments are running. Only exit the software if your LIS Drives are not performing remote controlled experiments currently. - Page 30 LIS Coordinator is properly connected to the software. The center gives information about the software version and the question mark symbol on the right can be clicked in order to open the LIS user guide. Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru,...
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Page 31: Connecting The Lis Coordinator
After connecting the LIS Coordinator, you can see which PanID it uses under “PanID” figure 12 Settings page In the status bar of the LIS Software you can also see the connection status of the coordinator: Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru,... -
Page 32: Connecting The Lis Drives
Connecting the LIS Drives If your LIS Drives are not configured yet to connect to the LIS Coordinator on the shown PanID, do following steps: On the LIS Drives go to “Settings” “Wireless” Set the “Zigbee PAN ID” to the same number the LIS Software is showing on the “Settings”... -
Page 33: Running An Experiment
Running an Experiment Before you can run remote experiments with the LIS system you need to define experiments. Each LIS Drive you want to use needs its own experiment. Go to the “Experiments” tab in the LIS Software ... - Page 34 Experiments” to prepare multiple experiments at a time The LIS Drive will now start pumping air out of the cartridge to create an initial negative pressure. It will stop pumping when correctly prepared. Now carefully remove the cartridge from the flask and remove the red luer plug.
- Page 35 The LIS Drive will now start your experiment. The status will change to “Running”. You can track the progress by checking the “Remaining Time / Total Time” or “Current Volume / Target Volume” After your experiment is finished, the status will change to “Finished”.
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Page 36: Create A New Feeding Profile
Once you are finished make sure to save the profile by clicking on “Save”. The profile will now be listed under “Profiles”. figure 13 feeding profile editor with exemplary combination of feeding segments Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru... -
Page 37: Add New Parameters
For example, the height of the water column or the density of the liquid inside the cartridge need to be considered. In order to use the LIS System with big shake flasks, needles with a female Luer connector can be connected to the tip of the LIS Cartridge. Thus, the position of the drop release can be moved downwards inside the shake flask, so that these drops do not stick to the wall of the shake flasks. -
Page 38: Charging The Lis Drive
USB. The LIS Drive and cables can be disinfected by wiping them softly with 70% ethanol wipes. Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru,... -
Page 39: Updating The Lis Drive Firmware
Updating the LIS Drive Firmware In case you want to update the firmware on the LIS Drive, ask the aquila biolabs service for the newest release. We will also provide you with a detailed instruction how to update the firmware. -
Page 40: Troubleshooting Guide
Long press (> 5 seconds) the knob to bootloader power off the drive. After turning it on LED blinks mode again it will be in normal operation mode alternating red and green Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru... - Page 41 Software as indicated in this user guide connected LIS Drive turned Turn on the LIS Drive LIS Drive not visible in LIS On the LIS Drive, go to “Settings” Wireless Software “Wireless” “Zigbee PanID” and set the deactivated on the LIS Drive...
- Page 42 Problem Possible causes Solution LIS Coordinator Connect the LIS Coordinator to the LIS not properly Software as indicated in this user guide connected Диаэм - официальный дилер продукции Aquila Biolabs в России, тел.: (495)745-0508, info@dia-m.ru, www.dia-m.ru...
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Page 43: Contact
Контактная информация сервисных центров Сервисный центр Диаэм в Москве: Адрес: 129345, г. Москва, ул. Магаданская, д.7, стр.3 Тел.: +7 (495) 745-05-08 (многоканальный) E-mail: service@dia-m.ru www.dia-m.ru Сервисный центр Диаэм в Новосибирске: Адрес: 630090, Новосибирск, Академгородок, пр. Ак. Лаврентьева, 6/1, офис 100А Тел.: +7 (495) 745-05-08 (многоканальный), +7 (383) 328-00-48 E-mail: service@dia-m.ru www.dia-m.ru...
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