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Related Manuals for strex STB-1400-04
Summary of Contents for strex STB-1400-04
- Page 1 STREX Cell Strain Instrument Catalog # STB-1400-04 User Manual...
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Page 2: Table Of Contents
Contents Section 1: Main Components..................3 Stretch Unit ........................3 Control Unit Front Panel ....................4 Control Unit Back Panel ....................4 Silicone Strain Chamber ....................5 Section 2: Use of the Cell Strain Instrument ..............6 Preparation of Cell Strain Instrument................6 System Operation ........ -
Page 3: Section 1: Main Components
Section 1: Main Components Stretch Unit Chamber length adjusting knob: Use the knob to adjust the distance of chamber brackets to maintain tension on the chamber. The bottom of the chamber should be taut. System coolant inlet and outlet valves: Be sure to supply cool water when in operation. -
Page 4: Control Unit Front Panel
Control Unit Front Panel Start and Stop Button: Use to start or stop the stretching action. Main Power Switch Water Pump Switch: Use to turn on and off the pump that circulates water to cool the motor. Strain Ratio: Use upper and lower buttons to increase and decrease stretch ratio Strain Frequency Selector: Use upper and lower buttons to adjust cycle frequency. -
Page 5: Silicone Strain Chamber
Silicone Strain Chamber (ST-CH-04.0) Chamber Dimensions 20mm x 20mm x 10mm Chamber membrane thickness = 0.1mm 4 places for pins... -
Page 6: Section 2: Use Of The Cell Strain Instrument
— using ethanol-immersed swabs. System Operation The STREX Cell Strain System needs to be water cooled when in operation. Without cooling, the maximum run time for the continuous operation of the motor is only 10 minutes. For longer operation, water cooling is essential. Also, please note that, when operating the unit in an incubator, the temperature inside the unit will be elevated so it is particularly important to ensure the cooling system is employed in this environment. -
Page 7: Culturing Cells In The Silicone Chambers
6. Make sure that the water pump is pumping water from the reservoir and through the Control and Stretch Unit. 7. Turn off Main Power and Water Pump Switches. Start Cell Stretching 1. The Power Switch must be OFF to freely rotate the Chamber Length Adjustment Knob. Silicone chambers containing cultured cells are attached to the Stretch Unit by inserting the 4 pins into the corners of the chamber. -
Page 8: Preparation Of Silicone Chambers
Preparation of Silicone Chambers Before using the chambers, they should be sterilized then coated with a cell adhesion matrix. The coating procedures below can be adapted for use with other matrices, such as elastin, pronectin, and laminin. Sterilize chambers in an autoclave for 20 minutes at 121° C. The silicone chambers can withstand temperatures up to 180°... - Page 9 Gelatin Coating Preparation of gelatin solution: 1. Add gelatin powder to PBS at a concentration of 2% 2. Autoclave the mixture to dissolve and sterilize Coating with gelatin solution: 1. Pour 3 ml of the gelatin solution into each strain chamber Incubate at 37 C for more than 30 minutes 3.
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Page 10: Section 3: Strain Parameters
Section 3: Strain Parameters (Stretch) LEFT Digit: Strain Frequency Selector RIGHT Digit: Strain Ratio Digit Program Description* Digit Degree of stretch Distance 60 Cycles/min Stretch 0.5 sec – contract 0.5 sec – repeat (1 Hz) 0.40 mm Sine wave 30 cycles/min (0.5 Hz) Stretch 1 sec –... -
Page 11: Section 4: Faq
Section 4: FAQ Q1: What are the characteristics of the silicone chamber? A1: The strain chamber is made from silicone elastomer consisting of polydiethylsiloxane as its major component. The chamber surface is strongly hydrophobic and cells have difficulty attaching to it; therefore, the chamber surface should be coated with an extra-cellular matrix like fibronectin, collagen, laminin, or gelatin before cultivation. - Page 12 Q4: How long can cells be stretched? A4: The duration depends on cell strain and condition. In general, two weeks cultivation in the incubator is possible with culture media changes every 2-3 days. It is also important to monitor reservoir coolant for the instrument motor. Insufficient amount of the cooling water may increase the temperature of the motor resulting in killing of cells and /or burning out the motor.
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Page 13: Section 5: References
Section 5: References Effects of repetitive stretch stimulation on neonatal rat cardiocytes in vitro, K. Kada, K. Yasui, K. Naruse, and J. Toyama. Environmental Medicine, 40: 69-72, 1996. Inhibitory action of repeated stretch stimulation on apoptosis in neonatal rat cardiocytes., K. Yasui, H. Shimano, K. - Page 14 Qiong-Yao, Folia Pharmacologica Japonica 124(3):301-310, 2004. 17. Mechanotransduction of integrin is essential for IL-6 secretion from endothelial cells in response to uniaxial continuous stretch, A. Sasamoto, M. Nagina. S. Kobayashi, K. Naruse, Y. Nimura, M. Sokabe, Am J Physiol Cell Physiol 288:1012-1022, 2005. 18.
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Page 15: Section 6: Safety Instructions
Section 6: Safety Instructions and Precautions Please read this section carefully before using the instrument. Items in this section alert the user to operational dangers that, if not followed, may damage the instrument or, more significantly, result in serious injury or death of the user. To ensure safe operation of the instrument, it is therefore imperative that you follow these instructions carefully. - Page 16 • We are not responsible for damages caused by malfunctions due to combinations of the cable. equipment or software not involving Strex. Do not plug many objects into a single electrical outlet since it may cause fire. • • • •...
- Page 17 • Do not exceed the rating of outlets and wiring equipment. If rating is exceeded with the multiple electrical components fire may be caused due to heat generation. • Do not touch the main unit or the power cable during severe weather events. It may Section 6: Safety Instructions and Precautions cause electric shock.
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Page 18: Section 7: Warranty
④ Damage caused by unavoidable external causes such as earthquakes, lightening, fire, flood, gas leak, power surges, or other acts of providence. 3. Strex is free from any responsibility for effects or loss or damages arising from the result of the machine operation.
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