1. Manuals
  2. Brands
  3. Fisher Bioblock Scientific Manuals
  4. Microscope
  5. Stemi 1000
  6. Operating manual

Fisher Bioblock Scientific Stemi 1000 Operating Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
Stemi 1000/2000/2000-C
Stereo Microscopes
Operating Manual
1 2

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Stemi 1000 and is the answer not in the manual?

Questions and answers

Summary of Contents for Fisher Bioblock Scientific Stemi 1000

  • Page 1 Stemi 1000/2000/2000-C Stereo Microscopes Operating Manual...
  • Page 2 Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service. ...

  • Page 3: Table Of Contents

    Stemi 1000, Stemi 2000, Stemi 2000-C TABLE OF CONTENTS Page Title Copyright Table of contents ..............
  • Page 4 Stemi 1000, Stemi 2000, Stemi 2000-C CARE AND TROUBLESHOOTING Care ..............

  • Page 5: List Of Figures

    Stemi 1000, Stemi 2000, Stemi 2000-C LIST OF FIGURES Page Figure 1-1 Principal assemblies of the Stemi 2000-C .......
  • Page 6 Stemi 1000, Stemi 2000, Stemi 2000-C Page Figure 3-1 Stemi 2000 with KL 1500 electronic ........

  • Page 7: Note

    Fig. 8 of Section 2, the T2 adapter is marked with the item number 3. • Refer to the annex for explanations of the abbreviations. • This instruction manual refers to the Stemi 1000, Stemi 2000 or the Stemi 2000-C microscope versions (see Page 1-9) and their options.

  • Page 8: General View

    Stemi 1000, Stemi 2000, Stemi 2000-C GENERAL VIEW (Basic unit) Stemi 1000 Stemi 2000 Stemi 2000-C 12/98 B 40-002 e...
  • Page 9 45 50 55 is now also available as a brand-new item in addition to the Greenough-type stereo microscopes Stemi 1000, Stemi 2000 and Stemi 2000-C. Unlike the Stemi 2000-C, the Stemi 2000-CS (”C” for ”Camera” and ”S” for ”Splitter”) features a fixed camera port with a 50/50 % beam splitting ratio, i.

  • Page 10: Notes On Device Safety

    Stemi 1000, Stemi 2000, Stemi 2000-C NOTES OF DEVICE SAFETY The Stemi 1000, Stemi 2000 or the Stemi 2000-C stereo microscope including original accessories or options must only be used for the microscopy applications described in this operating manual. The manufacturer cannot assume any liability for any other applications, possibly also involving indi- vidual modules or single parts.

  • Page 11: Description

    While the Stemi 1000 and Stemi 2000 are units for visual stereoscopic observation, with its trinocular tube the Stemi 2000-C offers all possibilities of micrographic and video image documentation.

  • Page 12: Description Of The Units

    Stemi 1000, Stemi 2000, Stemi 2000-C Description of the units The Stemi 1000, Stemi 2000 and Stemi 2000-C are highly powerful stereo microscopes that are dis- tinguished by compact and ergonomic design. Figure 1-1 shows the principal assemblies of the Stemi 2000-C.

  • Page 13: Figure 1-2 Optical Schematic Of The Stemi 2000-C

    Stemi 1000, Stemi 2000, Stemi 2000-C Figure 1-2 schematically shows the optical beam path in the Stemi 2000-C. The unit is switched over between the right tube connector and the camera output with the aid of an adjustable reflector group located under the tube system that features 100/0 % and 0/100 % intensities.

  • Page 14: Technical Data

    Stemi 1000, Stemi 2000, Stemi 2000-C Technical data (1) Dimensions and weight Mikroscope • Stemi 1000 Dimensions (width x depth x height) ........
  • Page 15 Stemi 1000, Stemi 2000, Stemi 2000-C Operation +15 to +35 °C Permissible ambient temperature ..........
  • Page 16 Stemi 1000, Stemi 2000, Stemi 2000-C Specimen stages, adjustment ranges "20 mm, rotatable by 360° Sliding stage ........... .
  • Page 17 Stemi 1000, Stemi 2000, Stemi 2000-C Adjustable eye distance ............

  • Page 18: Overview Of Modules

    Stemi 1000, Stemi 2000, Stemi 2000-C Overview of modules Basic microscope equipment Figure 1-3 Basic microscope equipment 12/98 B 40-002 e...
  • Page 19 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Microscope equipment 1000 2000 2000-C Stereo microscope Stemi 1000 consisting of • Stemi 1000 microscope body 455050 • Eyepiece W 10x/21 foc. 455042 • Attachment systems adapter (contained in 455050) •...

  • Page 20: Figure 1-4 Eyepieces/Eyepiece Cups/Reticules/Attachment Systems/Specimen Measuring Plate

    Stemi 1000, Stemi 2000, Stemi 2000-C Accessories (1) Replaceable optical systems Figure 1-4 Eyepieces/eyepiece cups/reticules/attachment systems/specimen measuring plate 12/98 B 40-002 e 1-10...
  • Page 21 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Replaceable optical systems 1000 2000 2000-C Eyepieces W 10x/21 foc. with eyepiece cup 455042 W-PL 10x/23 spectacle focusing 455043 W-PL 16x/16 spectacle focusing 455048 W 25x/10 foc. with eyepiece cup...

  • Page 22: Figure 1-5 Stands

    Stemi 1000, Stemi 2000, Stemi 2000-C (2) Stands 4.1.1 4.1.2 6.1.1 6.1.2 Figure 1-5 Stands 12/98 B 40-002 e 1-12...
  • Page 23 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Stands 1000 2000 2000-C Stand S, assy. containing: 455104 • Plastic plate, b/w 475290 9901 • Stage spring 473371 9902 Stand L, assy. (230 V) containing: 455110 • Lamp 10 467253 9902 •...
  • Page 24 Stemi 1000, Stemi 2000, Stemi 2000-C (3) Specimen stages Figure 1-6 Specimen stages 12/98 B 40-002 e 1-14...

  • Page 25: Figure 1-6 Specimen Stages

    Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Specimen stages 1000 2000 2000-C Sliding stage 455122 requiring: • Plastic plate, b/w ∅ 84 475290 9901 • Stage spring 473371 9902 Ball stage 455123 containing • Non-slip plate 475288 •...

  • Page 26: Figure 1-7 Halogen Lighting Systems For Vertical Illumination

    Stemi 1000, Stemi 2000, Stemi 2000-C (4) Halogen lighting systems for vertical illumination to 1.3 5 – 7 5 – 7 5 – 7 Figure 1-7 Halogen lighting systems for vertical illumination 12/98 B 40-002 e 1-16...
  • Page 27 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Halogen lighting systems for vertical illumination 1000 2000 2000-C Halogen lighting systems Built-in vertical illumination system 6 V 10 W 485001 9804 halogen (230 V) consisting of: • HAL 10 W socket for lamp 10 468043 9901 •...

  • Page 28: Figure 1-8 Cold Light Sources For Vertical Illumination

    Stemi 1000, Stemi 2000, Stemi 2000-C (5) Cold light sources for vertical illumination 1.4/2.3/3.5 4.4/5.4/6.3/7.3/8.3 1.3/3.2 4.1/5.1 3.4/4.3 3.3/4.2 to 4.1/5.1 10.2 10.1 10.2 10.2 10.1 10.1 Figure 1-8 Cold light sources for vertical illumination 12/98 B 40-002 e 1-18...
  • Page 29 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Cold light sources for vertical illumination (75 W) 1000 2000 2000-C Vertical illumination system with swan-neck fibre-optic conductor consisting of: • Swan-neck fibre-optic conductor, one-arm, 4.5 mm 417080 9001 fibre bundle, 500 mm long for KL 750 (D) •...
  • Page 30 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Cold light sources for vertical illumination (150 W) 1000 2000 2000-C Fit-on lighting system with fibre-optic conductor consisting of: • Flexible fibre-optic conductor with focusing attachment 455145 • Holder for built-in fibre-optic conductor for lamp mount 455143 •...

  • Page 31: Figure 1-9 Halogen Lighting Systems For Transmitted Illumination

    Stemi 1000, Stemi 2000, Stemi 2000-C (6) Halogen lighting systems for transmitted illumination 2.1.3 2.1.1 2.1.2 Figure 1-9 Halogen lighting systems for transmitted illumination B 40-002 e 12/98 1-21...
  • Page 32 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Lighting systems for transmitted illumination 1000 2000 2000-C (halogen) Option for transmitted illumination with plate ∅ 140 455137 containing: • Glass plate, transparent ∅ 84 475265 0001 Additionally required: •...

  • Page 33: Figure 1-10 Cold Light Sources For Transmitted Illumination

    Stemi 1000, Stemi 2000, Stemi 2000-C (7) Cold light sources for transmitted illumination 2.1.3 2.1.2 2.1.1 Figure 1-10 Cold light sources for transmitted illumination B 40-002 e 12/98 1-23...
  • Page 34 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Cold light sources for transmitted illumination 1000 2000 2000-C Option for transmitted illumination 455136 containing: • Glass plate, transparent ∅ 84 475265 0001 Additionally required: • Built-in fibre-optic conductor with focusing...

  • Page 35: Figure 1-11 Polarisation Systems

    Stemi 1000, Stemi 2000, Stemi 2000-C (8) Polarisation systems 1.5.2 1.5.1 1.1.2 1.1.4 1.1.1 1.1.3 1.2.2 1.2.1 Figure 1-11 Polarisation systems B 40-002 e 12/98 1-25...
  • Page 36 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Polarisation systems 1000 2000 2000-C Polarisation contrast in transmitted light consisting of: • Built-in vertical illumination system with fibre-optic conductor 1.1.1 – Built-in fibre-optic conductor with focusing 455145 attachment 1.1.2...

  • Page 37: Figure 1-12 Documentation Systems Using Photo Cameras

    Stemi 1000, Stemi 2000, Stemi 2000-C (9) Documentation systems using photo cameras Figure 1-12 Documentation systems using photo cameras B 40-002 e 12/98 1-27...
  • Page 38 Stemi 1000, Stemi 2000, Stemi 2000-C Order No. Stemi Stemi Stemi Documentation systems using photo cameras 1000 2000 2000-C CONTAX 167 MT reflex camera – – consisting of: • Reflex camera connection 2.5x for T2 456005 • Special adapter T2 for CONTAX 416010 •...

  • Page 39: Figure 1-13 Adapters For Video Cameras

    Stemi 1000, Stemi 2000, Stemi 2000-C (10) Adapters for video cameras Figure 1-13 Adapters for video cameras Order No. Stemi Stemi Stemi Documentation systems for video cameras 1000 2000 2000-C Standard C connection thread • TV camera 2/3” standard C connection...

  • Page 40: Function Elements

    Fixes the specimen Dust glass Prevents dust ingress and serves as a mount for a ring lamp and analysers; when using attachment systems with the Stemi 1000, it must be replaced by the so-called ”attachment system adapter” Lamp Illuminates the specimen...

  • Page 41: Figure 1-14 Function Elements Of The Stemi 2000-C

    Stemi 1000, Stemi 2000, Stemi 2000-C Item Designation Purpose/description Focusing drive Enables adjustment of the optical system to the specimen Max. adjustment range: 46 mm The left/right focusing drives are coupled mechanically; their ease of movement can be adjusted by means of the...
  • Page 42 Stemi 1000, Stemi 2000, Stemi 2000-C 12/98 B 40-002 e 1-32...

  • Page 43: Operation

    Stemi 1000, Stemi 2000, Stemi 2000-C Operation Unpacking and setting up The components belonging to the microscope are optionally supplied in transport containers or in a city box. 1 3 mm hexagon socket screw key 7 Stage inlay plates (2x)

  • Page 44: Figure 2-2 Unpacking

    Stemi 1000, Stemi 2000, Stemi 2000-C (1) Preparations • Remove the transport sleeve from the body of the microscope. • Remove the dust caps (2-2/1) from the tube openings. Figure 2-2 Unpacking (2) Setting up the stereo microscope • Place the microscope (2-3/1) on your work- bench.

  • Page 45: Commissioning

    Stemi 1000, Stemi 2000, Stemi 2000-C Commissioning Carry out the following work when commissioning for the first time. • Unpack and set up the unit as described in Section 2.1 . • The following adjustments have to be carried out before commissioning or as required.

  • Page 46: Working With Accessories

    Pay attention to the table in Section 1.3(4)). NOTE In the case of the Stemi 1000, in the ”attachment systems adap- Figure 2-6 Fitting an attachment system to the ter” supplied, instead of the dust Stemi 2000 glass.

  • Page 47: Figure 2-7 Mounting The Polariser

    Stemi 1000, Stemi 2000, Stemi 2000-C (3) Polarisation contrast in transmitted light • Insert the polariser (2-7/7) in the rotary stage (2-7/9) from below so that the dot marking on the polariser agrees with the line marking on the stage mount and secure it with two 2.0 mm hexagon socket screws (2-7/4).

  • Page 48: Figure 2-8 Mounting A Reflex Camera

    Stemi 1000, Stemi 2000, Stemi 2000-C (4) 35 mm photography with a reflex camera (e.g. CONTAX 167 MT) • Select the suitable T2 adapter (2-8/3) for the camera system used and screw it onto the connection for a reflex camera 2.5x for T2 (2-8/4).
  • Page 49 Stemi 1000, Stemi 2000, Stemi 2000-C (5) 35 mm photography with microscope camera MC 80 DX • Detach the dust cap (2-9/6) and insert the ∅ 30 microscope camera connection (2-9/5) in the camera tube and secure it with the hexagon socket screw (3 mm) (2-9/7).

  • Page 50: Figure 2-10 Mounting A Video Camera

    Stemi 1000, Stemi 2000, Stemi 2000-C (6) Video microscopy • Depending on the video camera used, adapt – Standard C connection thread (2-10/5) 0,5x 0,63x – ENG-bayonet connection (2-10/2) to the video camera (2-10/1) used. • Insert the pre-assembled unit in the camera...

  • Page 51: Fluorescence Contrast With Stereomicroscopes

    Stemi 1000/2000/2000-C/2000-CS Fluorescence contrast with stereomicroscopes Fluorescence contrast with stereomicroscopes Additional chapter for the following operating manuals: Stemi stereomicroscopes 1000/2000/2000-C/2000-CS and Stemi stereomicroscopes SV 6/SV 11/SV 11 Apo B 40-002 e 12/98...

  • Page 52: Fl S (Fluorescence For Stereomicroscope) Configuration

    Stemi 1000/2000/2000-C/2000-CS FL S configuration (Fluorescence for Stereomicroscopes) FL S (Fluorescence for Stereomicroscope) configuration 3.1.1 Name and intended application With the FL-S configuration, Carl Zeiss provides the possibility of performing fluorescence tasks with stereomicroscopes (regardless of their type). The features of fluorescence contrast can thus be combined with the benefits of the various stereomicroscope models (orthoscopy, wide object fields and long working distances).

  • Page 53: Configurations

    Stemi 1000/2000/2000-C/2000-CS FL S configuration (Fluorescence for Stereomicroscopes) Configurations Regardless of which stereomicroscope is used, different configurations are possible: No.. Description Cat.No. Comments FL S configuration with KL 1500 electronic, consisting of: 495054-9801-000 Schott cold-light source KL 1500 electronic (230 V) or...

  • Page 54: Fl S Configuration With Kl 1500 Electronic (Not Suitable For Uv Excitation)

    FL S configuration with KL 1500 electronic 3.2.1 FL S configuration with KL 1500 electronic (not suitable for UV excitation) Stemi 1000 / 2000 / 2000 C / 2000 CS Stemi SV 6 / SV 11 / SV 11 Apo...
  • Page 55 Attach microscope body (8/1) and, if required, safety ring (8/2) to the stand again. When using a Double Lens Vario microscope (Stemi 1000/ 2000), screw Allen screw (8/6) to the receptacle with dia. 53 using the screwdriver (8/5) to attach the barrier filter mount FL S (8/3).
  • Page 56 FL S configuration with HBO 50 or HBO 100 3.2.2 FL S configuration with HBO 50 or HBO 100 Stemi SV 6 / SV 11 / SV 11 Apo Stemi 1000 / 2000 / 2000 C / 2000 CS (with Stemi mount) (in Stemi mount)
  • Page 57 Stemi 1000/2000/2000-C/2000-CS FL S configuration with HBO 50 or HBO 100 Remove microscope body (with Stemi carrier) (11/2) and safety ring (11/3) from stand N (11/4). Attach jointed mount S (11/6) and FL S glare protector (11/5) to the stand column.

  • Page 58: Fl S Configuration With Lumatec Hbo 200

    FL S configuration with LUMATEC HBO 200 3.2.3 FL S configuration with LUMATEC HBO 200 Stemi SV 6 / SV 11 / SV 11 Apo Stemi 1000 / 2000 / 2000 C / 2000 CS (with Stemi mount) (in Stemi mount)
  • Page 59 Stemi 1000/2000/2000-C/2000-CS FL S configuration with LUMATEC HBO 200 Remove microscope body with Stemi mount (16/4) and safety ring (16/5) from stand N (16/3). Attach jointed mount S (16/1) and FL S glare protector (16/2) to the stand column. Then attach microscope body to the stand column again.
  • Page 60 Stemi 1000/2000/2000-C/2000-CS FL S configuration (Fluorescence for Stereomicroscopes) NOTES For installation of the light guide and for filter change, the focusing head must be separated from the mount. To unlock the focusing attachment, push it through the track until the end.
  • Page 61 Stemi 1000, Stemi 2000, Stemi 2000-C Care and troubleshooting Care Care of the stereo microscope is limited to the following: • Cover the unit with the dust cover after every use. • Do not place the unit in a humid room.
  • Page 62 Stemi 1000, Stemi 2000, Stemi 2000-C Troubleshooting and service Troubleshooting on the Stemis is limited to few activities: • Checking of the power supplies. • Checking of the illuminating devices. (1) Checking of the power supplies The checking of the power supplies is limited to the inspection of the fuses in: .
  • Page 63 Stemi 1000, Stemi 2000, Stemi 2000-C (2) Checking of the illuminating devices Check the illuminating devices including a check of the power supply units used (switch settings, mains connection) and if necessary, replace the halogen lamp. • Isolate the lighting unit (4-1/1) from the mains.
  • Page 64 Stemi 1000, Stemi 2000, Stemi 2000-C 12/98 B 40-002 e...
  • Page 65 Stemi 1000, Stemi 2000, Stemi 2000-C ANNEX – List of abbreviations ............. .
  • Page 66 Stemi 1000, Stemi 2000, Stemi 2000-C 12/98 B 40-002 e...
  • Page 67 Stemi 1000, Stemi 2000, Stemi 2000-C List of abbreviations B & L Bausch and Lomb Black & white Colour Television European standard Electronic News Gathering foc. Capable of focusing Free operating distance Halogen International Electrotechnical Commission International Protection International Organization for Standardization...
  • Page 68 Stemi 1000, Stemi 2000, Stemi 2000-C 12/98 B 40-002 e...

This manual is also suitable for:

Stemi 2000Stemi 2000-c

Table of Contents