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RWD D180P User Manual

Co2 incubator
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Summary of Contents for RWD D180P

  • Page 1 Incubator User Manual...
  • Page 2 RWD reserves the right to modify the product specifications without prior notice. RWD is not to guarantee the information in any forms, including (but not limited to) the guarantee responsibility of proposing the implied merchantability and suitability for a specific purpose.

  • Page 3: Table Of Contents

    Contents 1-Introduction ...................... 1 1.1 Overview .......................... 1 1.2 Safety ..........................1 1.3 Comprehensive description ..................... 1 1.4 Product features ......................2 1.5 Equipment environmental requirements ................ 2 1.6 Specification ........................2 1.7 Settable parameters ......................3 1.8 Product list ........................3 2-System safety ....................
  • Page 4 3.2 Product show and ports introduction ................16 4-Operation Instruction ..................18 4.1 Initial start ........................18 4.2 Menu ..........................18 4.3 Temperature and CO concentration settings .............. 19 4.4 Alarm settings ........................ 20 4.5 High temperature sterilization ..................21 4.6 Sensor calibration ......................

  • Page 5: 1-Introduction

    1.3 Comprehensive description The CO Incubator is an experiment box product designed, developed and produced by RWD, and also an advanced type of equipment for cell, tissue and bacteria culture. The CO Incubator ensures stable internal temperature, stable carbon dioxide level, constant pH, and high saturated humidity by the precise control systems for temperature, carbon dioxide and humidity, that to make the normal growth of cells.

  • Page 6: Product Features

    production, culture of hybridoma cells for antibody production, in vitro fertilization (IVF), stem cells, tissue engineering, and drug screening. 1.4 Product features  Triple sterilization function. Continuous sterilization for 24 hours, professional HEPA filtration system, and irregular sterilization;  Efficient, fast and easy high temperature sterilization cycle ensures not only effective disinfection and sterilization, but also automatic running at night.

  • Page 7: Settable Parameters

    × width × height: Capacity ~180L Weight ~120kg Heating method Inner wall heating, air casing 1.7 Settable parameters Parameters Detailed description Temperature 10.0-50.0°C concentration 0.0-20.0% Upper limit: (T+0.1) - (T+5.0) Temperature alarm threshold Lower limit: (T-0.1) - (T-5.0) Lower limit: (C-0.1) - (C-5.0) concentration alarm threshold Upper limit: (C+0.1) - (C+1.0) Lower limit: 80-100 %...

  • Page 8: 2-System Safety

    The following safety symbols and general marks may be used in the User Manual and on the equipment. If you have any question or suggestion regarding safety, please contact RWD for after-sales support. To prevent damages to the equipment and extend the service life of the equipment, please follow these instructions carefully.

  • Page 9: Safety Instructions

     The responsibility for equipment failure arising from any improper cleaning, maintenance and operation of the equipment should be borne by the user.  If the equipment host is disassembled without the authorization of RWD, RWD will no longer fulfill its commitments for quality assurance and technical maintenance services to the equipment.
  • Page 10 water mist should be wiped out.  The pressure value of carbon dioxide inlet should between 1- 2 atmospheres. (1Standard atmosphere =101.325kPa=1bar=14.5Psi）).  If the CO Incubator is overheated at 160°C, the internal temperature protector will cut off the heating power. In this case, it is necessary to restart the power supply after 8-12 hours of power-off.

  • Page 11: Product Assembly And Structure

    3. Product assembly and structure 3.1 Incubator Installation 3.1.1 Precautions before Installiation 1) At least 10 cm of clearance should be left behind the CO Incubator to allow the connection of electrical or gas supply devices to the equipment. In addition, at least 10 cm of ventilation clearance should be left on each side of the equipment.

  • Page 12: Install Vertical Plates And Ejector Rob Assembly

    Carbon dioxide sampling port Air duct 3.1.4 Install vertical plates and ejector rob assembly 1) Attach the provided buttonholes to the flanges on the rear side of each vertical plate (each plate is provided with 6 button holes). Button hole (3 on left and 3 on right) Vertical plate 2) Install two vertical plates with buttonholes into the CO Incubator, and...

  • Page 13: Install The Horizontal Shelve

    corresponding hanging piece of the vertical plate, and the position can be adjusted as needed. Pull the horizontal shelf bracket forward so that its front hole engages with the corresponding front hanging piece of the side vertical plate. Hanging slot Vertical plate Horizontal shelf bracket 4) Ejector rob assembly.

  • Page 14: Install The Access Port Filter

    3.1.6 Install the access port filter 1) Find an access port in the upper left corner of the inner side of incubator chamber. 2) Find the plug with filter from the accessory kit. 3) Push the opening cover to the right in the incubator and insert the filter assembly into the opening.

  • Page 15: Filling Of Humidity Pan

    3.1.10 Filling of humidity pan 1) Add sterile distilled water to the humidity pan until its water level is no more than 2/3 of total distance from the top of humidity pan. Place the humidity pan directly in the center of the bottom of the CO Incubator.

  • Page 16: Reverse Door Swing

    a 40-hour work week shall not exceed the eight-hour time weighted average of 5000 PPM (0.5% CO2).. The short-term exposure limit less than 15 minutes is 30,000 ppm (3% CO ). In confined areas, it is recommended to monitor carbon dioxide level due to carbon dioxide accumulation.
  • Page 17 2) Find 3 set screws at the back of the top cover and use a cross screwdriver to remove them, and then retain these screws. Combined cross screws 3) Push the top cover back from the front side and remove the cover. Top cover 4) Pull out the communication interface and front door heating film terminal at the positions shown below.

  • Page 18: Stacking Incubators

    Screw Wire cover 7) Use a screwdriver to remove 8 nuts on one side, remove the hinge and fasten these nuts and hinge securely on another direction. 8) Use screws to secure the wire cover, small cover and top cover to their original positions.
  • Page 19 Supporting feet Stacking rack...

  • Page 20: Product Show And Ports Introduction

    3.2 Product show and ports introduction Product real shot display...
  • Page 21 Component/interface name Function □ Power switch On/off □ Control panel Software operation □ USB data interface Export data information □ inlet cylinder connection port □ sampling port sampling within incubator □ Cooling pan Heat dissipation of equipment □ 220V Power outlet Power outlet □...

  • Page 22: 4-Operation Instruction

    4-Operation Instruction 4.1 Initial start The incubator will be start up only while it was installed completed with the power and gas supply properly connected, and the humidity pan filled with water. Press power switch to start the equipment and enter the main operation interface as shown below.

  • Page 23: Temperature And Co Concentration Settings

    4.3 Temperature and CO concentration settings Click the [Para SET] on the [Menu] to enter the parameter set interface, or click the “TEMP” or “CO ” area of the main interface to quickly enter the same interface [Para SET] interface supports the adjustment of temperature and CO concentration: a) The operating temperature range of incubator is: 10°C - 50°C;...

  • Page 24: Alarm Settings

    4.4 Alarm settings Click the “ALM SET” on the [Menu] to enter the interface as below, or click the “PCO /RH” area on the main interface to quickly enter the alarm settings interface; On this interface, the user can set the alarm limits of following parameters: Parameters Description of setting range Temperature...

  • Page 25: High Temperature Sterilization

    35°C, then the default upper limit of alarm is 35.5°C, and the adjustable range is 35.1°C to 40.0°C). 4.5 High temperature sterilization Necessary information to start high temperature sterilization  The sterilization cycle lasts for around 14 hours, of which 2-4 hours for heating, 2 hours for sterilization, and 6-8 hours for cooling;...
  • Page 26 3) The following page will be displayed during sterilization Note: Some materials may discolor during the process of high temperature sterilization. For example, stainless steel will show straw color after exposure to high temperature for a period of time, which is a normal phenomenon. Warning: The inner surface of the incubator will be heated to 140°C during the sterilization cycle.

  • Page 27: Sensor Calibration

    4.6 Sensor calibration After the equipment stably working, user could calibrate probes regularly as needed. The frequency of calibration depends on usage, environment conditions and accuracy requirements. Good laboratory specifications require at least one calibration of probes each year for the equipment. Click [Cali] on the [Menu] interface to enter the following interface: ...

  • Page 28: General

    4.7 General User can set the screen brightness, system language, silence duration, system time and password in the [General] interface. password set way is as follows:  In the factory settings, the password function is off by default;  The system default password is 0000;...
  • Page 29 export historical data according to the prompts. ALM Log The alarm record interface is shown as below, and the user can export the alarm record by clicking [Export]; Event The operation log interface is shown as below, and the user can also export the operation log by clicking [Export].

  • Page 30: Alarm And Prompt Message

    4.9 Alarm and prompt message Prompts Reason Solution The outer door is Opening time of outside door > Close the outside door open for too long! 2min; of the equipment. Please close the inner Opening time of inside door > Close the inside door door! 2min;...
  • Page 31 above alarm limit! concentration alarm threshold pressure valve cylinder is abnormal; 2） Adjust preset pressure of cylinder to 1.0-1.5bar. 1） Check whether the air pressure valve cylinder is abnormal; 2） Replace the cylinder Low CO pressure! pressure is lower than 0.6bar; and adjust the preset pressure of cylinder to 1.0-1.5bar;...
  • Page 32 personnel! 005 Communication error, please contact Communication between display Contact the after-sales after-sales panel and control panel failed staff; personnel! motor plugging, check whether there is any Motor error, foreign matter in the please check installation position of anything stuck Motor fault HEPA, contact...

  • Page 33: 5-Maintenance

    5-Maintenance 5.1 Preventive maintenance Operation Daily Weekly Monthly 3-6 months Annually Check the air pressure of the CO cylinder. Check the hinges and sealing gaskets of doors. Check whether the water level in the humidity pan is between 1/3 and 2/3 from the top Confirm and record CO humidity and temperature...

  • Page 34: Clean The Inside Of Incubator

    with alcohol, do not place them near open flames or other dangerous materials. During the operation of equipment, the internal temperature of CO sensor can be up to 150°C. The sensor should be cooled sufficiently before cleaning. Do not use highly alkaline or corrosive detergents.

  • Page 35: Clean The Humidity Pan

    Autoclaving of door should be avoided. inner Repairing corroded glass is extremely complicated. In most cases, glass must be replaced if it has been corroded. 5.5 Clean the humidity pan Clean the humidity pan with soapy water and regular laboratory disinfectants. Rinse them with sterile water and spray them with 70% alcohol.

  • Page 36: 6-Troubleshooting

    1) The wind blade of the fan motor falls off Contact after-sales staff of Loud noise 2) Fan motor shaft off-center RWD or replace the fan 3) Fan motor shaft, wind blade or motor turbine are stuck by a foreign matter Replace the fuse according...

  • Page 37: 7-Product Warranty

    If repair or replacement of parts is required, the cost will be borne by the user. If the reworked equipment was found to have been unauthorised disassembly, RWD will not provide after-sales service such as warranty, free maintenance and parts replacement. The warranty statement (including its restrictions) is exclusively issued by...
  • Page 38 RWD Life Science Web: www.rwdstco.com Add: 6540 Lusk Blvd, San Diego, CA 92121，USA Add: Room201, Robeta Building, No.11 High Tech North 4th Road, Nanshan District, Shenzhen City, Guangdong Province, 518057, China Tel: +001-858-900-6602 +86-755-86111286 After-sales Service: +86-755-86111281 After-sales E-mail: service@rwdstco.com...

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