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Table of Contents
Summary of Contents for Misonix SONICATOR XL2020
- Page 1 ® SONICATOR ULTRASONIC LIQUID PROCESSOR OPERATION MANUAL M I S O N I X MISONIX INCORPORATED MODELS XL2020 XL2015 XL2010 MISONIX INCORPORATED 1938 New Highway, Farmingdale, NY 11735 U.S.A. Telephone: 631-694-9555 Fax: 631-694-9412 Toll Free: 1-800-645-9846 www.misonix.com...
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Page 2: Table Of Contents
TABLE OF CONTENTS Safety - Warnings and Precautions Getting Started Inspection Power Requirements Placement of Equipment System Components Diagram Specifications for XL2020, XL2015 & XL2010 Description of Major Components Generator (Power Supply) Process Control Features of Model XL2020 Process Control Features of Model XL2015 Convertor (Transducer) Horn (Probe) Replacement Flat Tips... - Page 3 Stepped Microtip Extender Tip Sapphire Tipped Horn 9-6, 9-7 Sonabox Dual Horn Tray Horn Sonabox Diagram Troubleshooting 10-1 Overload Indicator Instructions 10-1 Troubleshooting Guide 10-1 Maintenance 11-1 General Maintenance 11-1 Cleaning Instructions 11-1 Tip Care 11-2 Applications 12-1 Principle of Ultrasonics 12-1 Processing Tissue 12-1...
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Page 4: Safety - Warnings And Precautions
Operation Manual ____________________Safety – Warnings and Precautions SAFETY - WARNINGS AND PRECAUTIONS Read ALL Instructions Before Installing or Using Equipment Your new Ultrasonic Liquid Processor has been designed, built and tested to assure maximum operator safety. However, no design can completely protect against improper usage which may lead to bodily injury and/or property damage. -
Page 5: Getting Started
Unpack the unit from its shipping carton and check the contents against the packing list. Before disposing of the packing material, check it carefully for small items. Report any missing components to Misonix immediately. Visually inspect all external controls, indicators and surfaces to detect any damage in transit. -
Page 6: Specifications For Xl2020, Xl2015 & Xl2010
** In a Vacuum area, additional cooling provisions may be needed. ACCESSORIES Use only accessories and probes listed in the Misonix catalog as suitable for use with this equipment. Do NOT attempt to fabricate ultrasonic tooling or accessories unless approval has been obtained from Misonix in advance. -
Page 7: Description Of Major Components
Operation Manual __________________________________Description of Major Components DESCRIPTION OF MAJOR COMPONENTS GENERATOR (POWER SUPPLY) Includes all operating controls and indicators, detachable three-wire grounded line cord, fuse, external power control jack and high frequency cable connector. PROCESS CONTROL FEATURES OF MODEL XL2020 •... -
Page 8: Convertor (Transducer)
Operation Manual _____________________Description of Major Components CONVERTOR (TRANSDUCER) Includes the electroplated housing, and black front driver (first stage of acoustic amplification) with a ½ - 20 threaded hole for horn attachment. A separate high-frequency cable is also furnished. HORN (PROBE) A 5"... -
Page 9: Function Of Controls And Indicators
Operation Manual ____________________Function of Controls and Indicators FUNCTION OF CONTROLS AND INDICATORS XL2020 FUNCTIONS POSITION CONTROLS FUNCTION Process Time Displays total pre-set time sonication is applied to sample (1 sec to 10 hours maximum). PULSAR ON Time Indicates pulse ON time from 0.1 seconds to 1 hour. Power Output Display Indicates relative percentage of maximum available ultrasonic power delivered to the horn (i.e. -
Page 10: Xl2010 & Xl2015 Functions
Operation Manual ____________________Function of Controls and Indicators XL2010 & XL2015 FUNCTIONS POSITION CONTROLS FUNCTION Overload Indicator Indicates a load condition greater than the maximum output power of the generator or that the unit requires tuning or other readjustments. Tuning Switch Activates tuning circuit. -
Page 11: Preparation For Use
Operation Manual ________________________________Preparation for Use PREPARATION FOR USE If the Ultrasonic Processor has been left in a very cold environment for a prolonged period of time, do not operate the unit until it has reached room temperature. Turn the power switch ON, with the Amplitude Control Knob at zero, for 20 minutes before using, to warm-up the electronics faster. -
Page 12: Removal And Attachment Of Horns And Tips
Retune the generator and try running the unit again. If the loud noise persists, or if you have difficulty tuning the unit, then contact MISONIX for assistance. _________________________________________________________________________________________________... -
Page 13: Tuning Instructions
Operation Manual _________________________________Tuning Instructions TUNING INSTRUCTIONS XL2020 TUNING INSTRUCTIONS (PROBES & HORNS) To assure proper operation, the generator must be tuned in accordance to the following procedure each time a new probe is changed. NOTE I. The probe or microtip should not be immersed in the liquid or come in contact with the work surface when tuning. -
Page 14: Xl2010 & Xl2015 Tuning Instructions (Probes & Horns)
Operation Manual _________________________________Tuning Instructions XL2010 & XL2015 TUNING INSTRUCTIONS (PROBES & HORNS) To assure proper operation, the generator must be tuned in accordance to the following procedure each time a new probe is changed. NOTE I. The probe or microtip should not be immersed in the liquid or come in contact with the work surface when tuning. - Page 15 Operation Manual _________________________________Tuning Instructions MICROTIP™ AND CUP HORN TUNING INSTRUCTIONS MICROTIPS & EXTENDERS Tuning of the horn should be done as quickly as possible. Running in air for a long period of time may damage the horn. Follow steps 1 through 3 in 7-1 for XL2020 or 7-2 for XL2010 & XL2015 Turn the Amplitude Control Knob (see 5-2 or 5-4) slowly towards setting 1.
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Page 16: Programming The Microprocessor Of Xl2020
Operation Manual _____________Programming the Microprocessor of XL2020 PROGRAMMING THE MICROPROCESSOR OF XL2020 REVIEW OF KEYS Action TUNE Activates or Deactivates tuning circuit. START Starts or Stops output in all modes of operation. Resets STOP timer in timed mode. PROG. Initiates Timer and PULSAR entry program and displays DATA entries on the LCD screen during run. -
Page 17: Continuous Mode
Ultrasonic energy is delivered continuously without any timing or pulsing cycles. The ultrasonic output will shut down when the total processing time reaches 9:59:59 (hr:min:sec). Programming Steps and Descriptions Action LCD Display SONICATOR XL2020 Turn ON generator. Press “I” on Power MISONIX INCORPORATED (Scrolls on screen, then switches to) Switch... -
Page 18: Timer Mode Only
Ultrasonic energy is delivered continuously for a specified length of time up to 9:59:59 (hr:min:sec). Programming Steps and Descriptions Action LCD Display SONICATOR XL2020 Turn ON generator. MISONIX INCORPORATED Press “I” on Power (Scrolls on screen, then switches to) Switch... - Page 19 00:00.1 to 59:59.1 (min:sec.tenths). Ultrasonic output will shut down when the total processing time reaches 9:59:59 (hr:min:sec). Programming Steps and Descriptions Action LCD Display SONICATOR XL2020 Turn ON generator. MISONIX INCORPORATED Press “I” on Power (Scrolls on screen, then switches to)
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Page 20: Timer And Pulsar Tm Mode
The range of pulse ON and OFF times can be entered from 00:00.1 to 59:59.1 (min:sec.tenths), with a maximum total processing time of 9:59:59 (hr:min:sec). Programming Steps and Descriptions Action LCD Display SONICATOR XL2020 Turn ON generator. MISONIX INCORPORATED Press “I” on Power (Scrolls on screen, then switches to) Switch... -
Page 21: Storing Programs Into Memory
The microprocessor can store up to 10 reproducible set-up programs as described in 8-2 to 8-5. A stored program can be reviewed by pressing the corresponding numerical key. Programming Steps and Descriptions Action LCD Display SONICATOR XL2020 Turn ON generator. Press “I” on Power MISONIX INCORPORATED (Scrolls on screen, then switches to) -
Page 22: Accessories
Operation Manual____________________________________________________________Accessories ACCESSORIES CUP HORN Attach directly to the convertor in place of the standard horn. The Cup Horn has either a 2" or 2½” diameter radiating surface with an attached clear acrylic sleeve. The sleeve of the Cup Horn is filled with water (or other liquid) and sample vessels are placed inside. The Cup Horn is used to process multiple samples without direct probe contact, and functions much like a high intensity ultrasonic bath. -
Page 23: Operation
Operation Manual____________________________________________________________Accessories For maximum transmission of acoustic energy into a vessel, use a cavitation-resistant fluid such as a water-glycol mixture, pure ethylene or propylene glycol. The sonication intensity will be greatly increased, in proportion to the glycol concentration. OPERATION Most laboratory grade plastic or borosilicate glass vessels which will fit into the cup are suitable for sonication. -
Page 24: Maintenance
The horn is a tuned resonant body of titanium alloy. Do not attempt to resurface the horn if it shows erosion after extended use. The horn may be used as long as it can be tuned. Contact Misonix if problems occur. CONTINUOUS FLOW CELL Used for continuous in-line processing applications, and for batches larger than 2 liters. - Page 25 Operation Manual____________________________________________________________Accessories Similarly, wet the -220 size O-ring in the other end of the body and the mating surface of the Disruptor horn and fit the body over the Disruptor horn. Screw the body on the Disruptor horn until it bottoms and then back it off ¼ turn. The body should not be tightened on the horn.
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Page 26: Microtip Tm
Operation Manual____________________________________________________________Accessories MICROTIP Attach directly to the end of the ½” diameter tapped horn in place of the flat tip. The Microtip Probes are tapered down to a narrow point and serve as the third stage of acoustic amplification. They are used to process small sample volumes ranging from 0.2 - 50 ml. -
Page 27: Extender Tip
Eroded discs can NOT be refinished or polished. Cracked, broken or eroded discs can be removed from used horns and replaced at the factory. Contact MISONIX or local representative for current replacement information. Horns with broken or chipped discs should never be used. Erosion could occur on the exposed face of the horn and render it unsalvageable. -
Page 28: Sonabox
Operation Manual____________________________________________________________Accessories SONABOX Before unpacking or assembling, please READ THESE INSTRUCTIONS carefully. See 9-8. Unpack by grasping the Enclosure body to lift from carton. DO NOT lift by latch assembly (door handle). Open SONABOX by lifting latch handle to the horizontal position and turning handle 90 in either direction. -
Page 29: Troubleshooting
Check horn for cracks or excessive erosion. ® Turn on SONICATOR and follow tuning instructions. If horn/tip cannot be tuned, replace and tune again. If all preceding fails to rectify problem, consult Misonix, Inc. TROUBLESHOOTING GUIDE SYMPTOM POSSIBLE CAUSE REMEDIAL ACTION... -
Page 30: Maintenance
Make certain the cable always has slack and is never tensioned. Move the generator or convertor assembly closer to one another to accomplish this. If this is not possible, contact Misonix to obtain a longer cable. ®... -
Page 31: Tip Care
Operation Manual______________________________________________________________Maintenance TIP CARE The probe tip may be sterilized either by immersing in boiling water or in a detergent bactericide and a disinfectant. Before each experiment, and after tuning, place the probe tip in water or alcohol and turn the power on for a few seconds to remove residue. -
Page 32: Applications
Operation Manual_______________________________________________________________Applications APPLICATIONS PRINCIPLES OF ULTRASONICS The generator (power supply) converts conventional 50/60 Hz AC line power to 20 kHz electrical energy which is fed to the convertor where it is transformed to mechanical vibration. The heart of the convertor is a piezoelectric crystal which, when subjected to an alternating voltage, expands and contracts. -
Page 33: Processing Difficult Cells
Operation Manual____________________________________________________________Applications PROCESSING DIFFICULT CELLS When processing difficult cells, the use of enzymes should be considered. Glusulase can be used with yeast, lysostaphin can be used with staphylococcus, collagenase can be used with skin and cartilage, and trypsin hyaluronidase can be used with liver and kidney. -
Page 34: Techniques
Operation Manual____________________________________________________________Techniques TECHNIQUES PROBE DEPTH Immerse the probe tip not less than 1 to 1½ times the tip diameters into the solution, without touching the bottom. This depth rate applies to the ½" horn; immersion depth can be less for larger horns and may have to be more for smaller probes used at higher intensity. -
Page 35: Warranty
The obligation of MISONIX Incorporated under this guaranty will be to make any repair at its plant in Farmingdale, New York, or at any of its designated service centers, without charge, including parts and labor, but not including shipping charges, necessitated by any defect in workmanship or material. -
Page 36: Return Of Equipment
IMPORTANT NOTICE BY RETURNING ANY MATERIAL TO MISONIX INC., THE CUSTOMER OR THE CUSTOMER'S AGENT THUS CERTIFIES THAT ANY AND ALL MATERIALS SO RETURNED ARE, OR HAVE BEEN RENDERED, FREE OF ANY HAZARDOUS OR NOXIOUS MATTER OR RADIOACTIVE CONTAMINATION AND ARE SAFE FOR HANDLING UNDER NORMAL REPAIR SHOP CONDITIONS. -
Page 37: Literature Request
Operation Manual__________________________________________________________Literature Request LITERATURE REQUEST Available from Misonix is descriptive material on ultrasonic liquid processing. To order, place a check next to the title you with to receive. (Quantity limit is one copy per title) BIBLIOGRAPHIES: BIB-1 Action of Ultrasonics on Living Tissue... -
Page 38: Published Papers
TN-7 Development of Protocols TECHNICAL BULLETINS: TB-1 Ultrasound, Hearing and Health TB-3 Sifting Solids TB-4 Pulsed Sonication TB-5 ASTM Test Method G-32 Mail Request To: MISONIX, INCORPORATED Literature Request 1938 New Highway Farmingdale, NY 11735 Ship Literature To: Name: _________________________... - Page 39 Operation Manual_____________________________________________Function of Controls and Indicators XL2010 & XL2015 CONTROLS AND INDICATORS DIAGRAM OVERLOAD PULSAR TUNE DUTY CYCLE %/1 sec Control Index 1. Overload Indicator 2. Tuning Switch 3. Power Switch 4. Power Output Display 5. PULSAR Cycle Timer (XL2015 Only) 6.
- Page 40 Operation Manual_____________________________________________Function of Controls and Indicators XL2020 CONTROLS AND INDICATORS DIAGRAM 40 50 100% T U N E Control Index 1. Process Time 9. LCD Display Screen 17. START/STOP Key 2. PULSAR On Time 10. Elapsed Time Display 18. Amplitude Control Knob 3.
- Page 41 Operation Manual_________________________________________________________Preparation for Use CONVERTOR MOUNTING DIAGRAM _________________________________________________________________________________________________...
- Page 42 Operation Manual_______________________________________________________________Accessories SONABOX DIAGRAM S U P E R S O N A B O X A S S E M B L Y F O R S T A N D A R D H O R N S S U P E R S O N A B O X A S S E M B L Y F O R C U P H O R N U S E _________________________________________________________________________________________________...
- Page 43 Operation Manual____________________________________________________________Getting Started SYSTEM COMPONENTS DIAGRAM 1. Operating Manual 2. High Frequency Cable 3. 7/16” X ½” Open End Wrench 4. Generator 5. Convertor 6. Power Cord 7. (2) Pin Spanner Wrenches 8. ½” Removable Flat Tip 9. ½” Tip Diameter Horn _________________________________________________________________________________________________...
- Page 44 Operation Manual_________________________________________________________Preparation for Use REMOVAL AND ATTACHMENT OF HORNS AND TIPS DIAGRAM Horn Removal Horn Attachment Tip Removal Tip Attachment Microtip Removal Microtip Attachment (1) 7/16” X ½” Open End (2) Pin Spanner Wrenches _________________________________________________________________________________________________...
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