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Summary of Contents for tecan Te-VacS
- Page 1 Operating Manual Tecan Vacuum Separator (Te-VacS™) Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0...
- Page 2 2010-09-23 Changes: Introducing markers for Te-VacS tubings; various minor corrections 2017-08-30 Update: Te-VacS also an option for Fluent instru- ment © 2017, Tecan Trading AG, Switzerland, all rights reserved. Information contained in this document is subject to change without notice.
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Page 3: Table Of Contents
2.4.2 Key Operator................... 13 2.5 Decontamination Declaration................ 14 3 Technical Data ...................... 15 3.1 Type Plate...................... 15 3.2 Dimensions and Weights .................. 16 3.3 Power Consumption ................... 17 3.4 Vacuum Conditions..................... 17 3.5 Environmental Conditions................... 17 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 3 / 54... - Page 4 5.2.3 Preparing the Tubing ................ 29 5.2.4 Connecting the Waste Bottle .............. 30 5.2.5 Connecting an Additional Waste Bottle........... 31 5.2.6 Connecting the Base Plate .............. 32 5.2.7 Tubing Identification................ 33 4 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0...
- Page 5 9.3 Tubing and Cables.................... 47 9.4 Base Plate Vacuum Block .................. 48 9.5 Spacers....................... 48 9.6 Vacuum Blocks .................... 49 9.7 Accessories ...................... 50 10 Customer Support .................... 51 10.1 Contacts...................... 51 Abbreviations...................... 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 5 / 54...
- Page 6 Table of Contents 6 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0...
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Page 7: About This Manual
• The Te-VacS should not be used in the absence of functional safety devices. Only liquids suitable for the Te-VacS, with respect to the chemical resistance of the liquid system and explosion/fire hazard, may be used. Special applications requiring the use of hazardous substances are run at the user’s own risk. -
Page 8: Warranty
Warranty 1.5 Warranty The Te-VacS must not be used with components that are not approved by Tecan. The use of unapproved components may impair the safety concept of the Te-VacS. The use of unapproved components would invalidate any warranty of safety and compliance to national and international standards, as required for UL/CSA certification, by EC directives, etc. - Page 9 1 - About This Manual Compliance with Laws and Standards Refer to the corresponding Operating Manual of the instrument. Refer to section “Reference Documents” [} 8]. Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 9 / 54...
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Page 10: Document Conventions
Prerequisites appear as follows—e.g.: P “General Safety Information” has been read. Tips Additional tips appear as follows—e.g.: For safety conventions and symbols refer to section “Safety” [ 11]. 10 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 11: Safety
2 - Safety Safety Message Conventions 2 Safety This chapter describes the safety concept of Te-VacS, provides general rules of correct behavior, and warnings concerning hazards associated with the use of the Te-VacS. 2.1 Safety Message Conventions 2.1.1 Signal Words... -
Page 12: General Safety Information
Te-VacS is designed and built in accordance with the present state-of-the-art tech- nology and the recognized technical safety regulations. Nevertheless, risks to users, property and the environment can arise if the Te-VacS is used without due care and attention. The safety of all users and personnel depends on the strict observation of these safety instructions and awareness of the safety-related warnings provided in this manual. -
Page 13: Operating Company
Observe all safety instructions of the instrument the Te-VacS is used on. 2.3 Operating Company The operating company must ensure that the Te-VacS and in particular the safety features, function properly and that all the personnel in contact with the instrument are adequately trained. -
Page 14: Decontamination Declaration
“Decontamination” [} 38]. Tecan reserves the right to refuse to deal with any Te-VacS or its parts or accessories that is not accompanied by the Decontamination Declaration form. 14 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 15: Technical Data
Type Plate 3 Technical Data 3.1 Type Plate Illustration 1: Location of the type plate The type plate is on the rear side of the Te-VacS and contains the following information: Identification data Model REF: Ordering information (material number and revi-... -
Page 16: Dimensions And Weights
The base plate vacuum block extends over seven grid spaces (a) on the Freedom EVO worktable as well as on the Fluent deck. Component Weight Te-VacS tower <13.0 kg (<28.7 lbs.) Waste bottle 2.0 kg (4.4 lbs.) Base plate vacuum block 3.3 kg (7.3 lbs.) 16 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 17: Power Consumption
Max. pressure difference 700 mbar 3.5 Environmental Conditions Te-VacS is intended for indoor operation and storage only. For detailed information on operating conditions refer to the Operating Manual of the instrument the Te-VacS is used with. Refer to section “Reference Documents” [ 8]. -
Page 18: Chemical Resistance
3 - Technical Data Chemical Resistance 3.6 Chemical Resistance The following tables contain the chemical resistance of Te-VacS components to some frequently used liquids and vapors. The tables below have been compiled from producer information. They are not comprehensive and are updated on the basis of new information available from the producers. -
Page 19: Chemical Resistance To Vapors
3.7 Emissions Refer to the Operating Manual of the instrument. Refer to section “Reference Documents” [} 8]. 3.8 Software Requirements The Te-VacS may be operated by the following software packages: • Freedom EVOware • FluentControl • Other Tecan software packages supporting Te-VacS It is strongly recommended to use the latest software version. -
Page 20: Description Of Function
4 - Description of Function Overview 4 Description of Function This chapter explains the basic principle of the Te-VacS, shows how it is structured and gives a functional description of the assemblies. 4.1 Overview The Te-VacS cannot be operated as a stand-alone system, but only as a device integrated into a Tecan instrument. -
Page 21: Te-Vacs Tower
4 - Description of Function Te-VacS Tower 4.2 Te-VacS Tower The control unit and the vacuum unit are assembled in the Te-VacS tower. A sound absorber (A) can be mounted directly on the TeVacS tower. Illustration 4: TeVacS tower with sound absorber 4.2.1 Control Unit... -
Page 22: Base Plate Vacuum Block
Plate waste (B): Ramp for the disposal of used labware. 4.7 Operating Elements and Displays The Te-VacS has no operating or display elements of its own. It is switched on and off by means of the main switch of the instrument. All commands and feedback information are handled via the application or setup and service software modules and their interfaces. -
Page 23: Function
The vacuum can be released via ventilation valves (H). To compensate for variations of ambient pressure in different altitudes and weather conditions, the Te-VacS pressure control system is based on pressure difference measurement. The following illustration shows an example of pressure difference. The current ambient pressure is supposed to be 1000 mbar. -
Page 24: Basic Principle Of An Extraction Process
Crude mixture Resin or filter Collection plate Single well of collection plate Purified product Spacer Low pressure atmosphere Base plate vacuum block To the waste bottle Vacuum block 24 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 25: Pumping Procedures
With the soft pumping procedure the whole vacuum path is pumped (i.e., the extraction valves are open from the start). This results in a slow pressure drop in the vacuum block. Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 25 / 54... - Page 26 The soft pumping procedure is applied if • slow processing is desired, • the substances require long reaction times, • delicate contents (filters) are placed in the separation columns. 26 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0...
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Page 27: Operation
5 - Operation Process Validation 5 Operation This chapter describes how the Te-VacS is installed and gives instructions on initial operation. 5.1 Process Validation For all applications of the Tecan instrument, the user must ensure that the requirements of each protocol are carefully observed. -
Page 28: Preparing The Waste Bottle
3. Ensure that the sealing (B) is properly placed in the bottle cap. 4. Manually tighten the bottle cap. 5. Clip the waste bottle to the bottle holder of the Te-VacS tower. 28 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 29: Preparing The Tubing
2. Ensure that there is a fixing ring (B) in each fixing nut (C). NOTICE! Otherwise the tubing (D) is not tight. Illustration 15: Tubing preparation Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 29 / 54... -
Page 30: Connecting The Waste Bottle
(A, B, C). Illustration 16: Waste bottle connections 2. Connect the liquid level sensor cable to the Te-VacS tower (D). 3. Carefully tighten the tubing’s fixing nuts using a wrench. NOTICE! It is recommended to tie the tubes together using cable ties. -
Page 31: Connecting An Additional Waste Bottle
4. Connect the liquid level sensor cable (E) to the vacuum unit. 5. Carefully tighten the tubing’s fixing nuts using a wrench. NOTICE! It is recommended to tie the tubes together using cable ties. Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 31 / 54... -
Page 32: Connecting The Base Plate
Te‑VacS tower (C, D) using two tubes of the same length. 3. Carefully tighten the tubing’s fixing nuts using a wrench. NOTICE! It is recommended to tie the tubes together using cable ties. 32 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 33: Tubing Identification
Illustration 19: Color markers (clips and stickers) 2. Attach the clips (A) to the connected tubes according to the colors of the stickers on the Te-VacS tower. Slide the clips from the tool onto the tubes as shown in the illustration below. -
Page 34: Mounting The Sound Absorber
2. Screw on the sound absorber (B) to the exhaust outlet of the vacuum unit. Illustration 21: Mounting the sound absorber 3. Carefully tighten the fixing nut using a fork wrench. 34 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 35: Exchanging The Sheet Metal Frame Of The Vacuum Block
3. Put the spare sheet metal frame (B) onto the vacuum block and fix it by means of the 10 hexagon socket head screws. Illustration 22: Vacuum block with spare sheet metal frame Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 35 / 54... -
Page 36: Using The Correct Spacer For A Given Application
3. Calculate the required spacer height (H Spacer For vacuum blocks A and B: = 46 mm - H Spacer For vacuum blocks C and D: = 71 mm - H Spacer 36 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 37: Running A Method
Table 4: Available spacers 5.3 Running a Method The application software that is used for running methods is described in the corresponding Application Software Manual. Refer to “Reference Documents” [} 8]. Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 37 / 54... -
Page 38: Preventive Maintenance And Repair
“Decontamination Declaration” [} 14]. WARNING Contamination! Substance residues on the Te-VacS can cause personal injury and affect the in- tegrity of the process. • Decontaminate the Te-VacS and its parts and accessories before any interac- tion. -
Page 39: Maintenance Schedule
Te- cially available VacS” [} 41]. mild detergent or a decontamination solution as clean- ing agents. Tubing connec- Check – – tions Table 5: End of day maintenance Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 39 / 54... -
Page 40: Monthly Maintenance Table
Sealing is crystalized on the surface, clean and lubri- cate the sealing with sil- icone grease. Table 6: Monthly maintenance See also "Reference Documents" } 8 40 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 41: Maintenance Tasks
2. Clean the ventilation holes (A) and extraction holes (B) in the base plate vacuum block and remove any obstructions. Illustration 23: Ventilation and extraction holes in the base plate vacuum block Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 41 / 54... -
Page 42: Troubleshooting
Tecan instrument used. Re- fer to section “Reference Docu- ments” [} 8]. Instrument does Technical damage Please consult the “Customer not recognize the Support” [} 51]. Incorrect setup Te-VacS 42 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... - Page 43 Tecan instrument used. Decreasing pump Salts crystallized in the pump If possible, use solutions with capacity head lower salt concentration. Please consult the “Customer Support” [} 51]. Table 7: Te-VacS troubleshooting table Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 43 / 54...
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Page 44: Packaging, Transport, Storage And Disposal
This chapter includes regulatory information about recycling and labels on packaging that needs to be followed. NOTICE Prevent damage by unqualified and unauthorized personnel! Packing, unpacking, transport and storage may only be performed by Tecan per- sonnel or personnel authorized by Tecan! • Please consult the “Customer Support”... -
Page 45: Disposal
Recycling in accordance with applicable legal regulations! Observe the laws applicable in your country for recycling. For further information refer to the Operating Manual of the instrument. Refer to section “Reference Documents” [} 8]. Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 45 / 54... -
Page 46: Spare Parts And Accessories
Te-VacS Tower 9 Spare Parts and Accessories This chapter lists spare parts and accessories that are needed for maintenance and repair of the Te-VacS including their corresponding ordering information. How to Order • Look up the ordering information in the tables. -
Page 47: Waste Bottle
TUBE PTFE 6*8MM 12M TE-VACS (length = 12.5 m) Tubing 4 x 1 mm PTFE 10760915 TUBE VACUUM PTFE 4*6MM 2.5M (length = 2.5 m) Table 11: Tubings, tubing connectors and cables for the Te-VacS Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 47 / 54... -
Page 48: Base Plate Vacuum Block
PLATE BASE VACUUM BLOCK vacuum block TE-VACS Positioning pin 10760640 PIN POSITIONING BASE PLATE VACUUM BLOCK Table 12: Spare Parts for the Te-VacS base plate vacuum block 9.5 Spacers Plain Text Designa- SAP No Label Designation tion Spacer no 1, height 10760663 SPACER NO.1 H7.5MM SPE TE-... -
Page 49: Vacuum Blocks
Material PMMA (non chemical resis- tant, transparent). For top skirted cartridges from Qiagen, Promega and Macherey-Nagel. Comes with hard seal- ing (10760638, mounted) and soft sealing (30021773). Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 49 / 54... -
Page 50: Accessories
SLIDE WASTE TE-VACS-B/TE- VACS-C Paper holder (for filter 10760653 HOLDER FILTER PAPER paper) 10760656 Filter paper, ca. 8.5 cm 10760656 FILTER PAPER 125MM*85MM x 12.5 cm 10760653 Table 15: Accessories for the Te-VacS 50 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0... -
Page 51: Customer Support
This chapter informs you how to contact us in case help is needed. It lists addresses and telephone numbers of the manufacturer’s representatives. Tecan and its representatives maintain a fully trained staff of technical specialists around the world. For any technical question, contact the nearest Tecan representative. - Page 52 E-mail helpdesk.benelux @tecan.com Netherlands Scandinavia Tecan Nordic AB Phone +46 8 750 39 40 Sveavägen 159, 1tr +46 8 750 39 56 SE-113 46 Stockholm E-mail info@tecan.se Sweden 52 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0...
- Page 53 +1 408 953 3100 (Tecan Sys- 2450 Zanker Road Toll Free: tems) San Jose, CA 95131 +1 800 231 0711 +1 408 953 3101 E-mail tecan-sy@tecan.com Table 16: Customer Support contacts Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0 53 / 54...
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Page 54: Abbreviations
Field Service Engineer Tecan vacuum separator WEEE International Electrotechnical Com- mission Waste Electrical and Electronic Equipment installation qualification International Organization for Stan- dardization MALDI Matrix Assisted Laser Desorption Ion- ization 54 / 54 Tecan Vacuum Separator (Te-VacS™) Operating Manual, 391236, en, V4.0...
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