Page 1 Mya 4 Reaction Station Instructions January 2020 Issue 8...
Page 2: Table Of Contents
Mya 4 Reaction Station – Instructions Contents 1 Introduction 2 Mya 4 Standard Warranty 2.1 Mya 4 Warranty Exclusions 3 Safety information 4 Important warnings 5 Component guide 6 Set-up and operation 6.1 Requirements 6.2 Getting started 6.3 Set-up overview 6.3.1 Connecting the Reaction Station to the mains power and the Control Pad...
Page 3 Mya 4 Reaction Station – Instructions 6.7.10 Attaching glass condensers to the Manifold Head 6.7.11 Important tips for optimum refluxing 6.8 Stirring options 6.8.1 Magnetic stirring 6.8.2 Overhead stirring (also called mechanical stirring) 6.9 Mya 4 reaction vessel options 6.10 Mya 4 inserts 6.11 Reflux tubes and multi-neck lids...
Page 4 Mya 4 Reaction Station – Instructions 7.6.2 Service Settings tab 7.6.3 PID Gains tab 7.6.4 Network Share tab 8 Safety 8.1 Chemical resistance 8.2 Electrical safety 8.3 Temperature safety 8.3.1 Printed Circuit Board (PCB) sensors 8.3.2 Block temperature (Tb) sensors 8.3.3 Coolant temperature (Tc) sensors...
Page 5: Introduction
• 2 ml to 400 ml • Software control www.radleys.com/products/our-products/mya-4-reaction-station Radleys accepts no liability if users do not observe the instructions for the safe operation of the Mya 4 Reaction Station as set out in this instruction manual. Issue 8 Page: 5...
Page 6: Mya 4 Standard Warranty
Mya 4 Standard Warranty The Mya 4 Standard Warranty is included free of charge as part of your Mya 4 Reaction Station purchase. It ensures that you have full remote and on-site support for the first 12 months of your Mya 4 experience.
Page 7: Mya 4 Warranty Exclusions
Mya 4 Reaction Station – Instructions Mya 4 Warranty Exclusions The following are excluded from the Mya 4 Standard Warranty: • Repairs and parts outside the 12 month warranty period (including the cost of labour, travel, accommodation, subsistence and shipping): →...
Page 8: Safety Information
Mya 4 Reaction Station – Instructions Safety information The following comments are included to assist the user in the safe and efficient operation of the Mya 4 Reaction Station. Warning  Applies when there is a possibility of personal injury or damage to equipment.
Page 9: Important Warnings
• take care not to touch the aluminium inserts or reaction vessels during and after heating/cooling, as there is the risk of burns from hot or extreme cold surfaces (Mya 4 inserts can remain hot for a considerable time after the heating source has been switched off),...
Page 10: Component Guide
Mya 4 Reaction Station – Instructions Component guide Front view Rear view Issue 8 Page: 10...
Page 11: Set-Up And Operation
Turn on the gas source(s). Turn Mya 4 on using the on/off switch on the side of the instrument. Wait for Mya 4 Control Pad to boot up, and display front screen. See Section 7 on page 79. Issue 8...
Page 12: Connecting The Reaction Station To The Mains Power And The Control Pad
Plug the other end into the mains supply. Step 2 Plug one end of the Mya 4 Control Pad cable into the controller socket at the rear of the unit. Ensure the cable is fully secured using the securing screws.
Page 13: Coolant Supply
Mya 4 Reaction Station – Instructions Coolant supply Mya 4 requires a liquid coolant supply to be connected and running at all times when the unit is in operation. The coolant flows through Mya 4’s internal heat exchangers, which are located in each reaction zone, to dissipate heat from the cooling Peltier cells.
Page 14 Mya 4 Reaction Station – Instructions Notes → To ensure correct operation of Mya 4, it is important to control and monitor the coolant flow within the stated operating limits at all times by using the coolant flowmeter (RR40633) supplied.
Page 15: Optimising Cooling Performance
However, the female connectors at the rear of the Reaction Station do not have shut-off valves. If you need to move Mya 4 to a different location, use the blanking plugs (see Step 1 above) to block these connectors off during transportation.
Page 16 Mya 4 Reaction Station – Instructions Notes → Coolant must be flowing at all times when running an experiment, even when reactions are not being cooled. → If the coolant supply is not switched on before an experiment is started, or the flow rate is too low, an error will be detected, a warning displayed and heating/cooling will stop.
Page 17: Internal Gas Purge
RR40634 Notes → To ensure correct operation of Mya 4, it is important to control and monitor the purge gas flow within the stated operating limits at all times by using the gas flowmeter (RR40632) supplied. → All tubing should be fully secured using a tubing clamp (RR40634). Slide the tubing clamp over the end of the tubing before connecting the tubing to a connector.
Page 18 Mya 4, securing with a tubing clamp. Notes → Mya 4 is not hermetically sealed, therefore the inert/dry gas will escape from the unit into the atmosphere. Warning  Operating Mya 4 without an inert/dry gas purge can damage the instrument and will void the warranty.
Page 19: Internal Drain
→ If you need to move Mya 4, it is recommended that you first slightly tilt the unit from left to right, and front to back, to ensure that all residual fluid is removed.
Page 20: Reflux & Manifold Heads
Reaction Station, • distribute inert gas to each position, • minimise solvent evaporation and allow for refluxing. Two head options are available for Mya 4: a Reflux Head or a Manifold Head. Reflux Head Manifold Head Reflux Head Provides fully integrated reflux operation without the need for additional condensers when used with reaction vessels and reflux tubes.
Page 21 Mya 4 Reaction Station – Instructions RR40816 RR40556 Tubing and connectors Coil condenser for Mya Manifold Head to glass condenser multi-neck lid Notes → You will need to attach the Manifold Head if you are using multi-neck lids. (See page 59 for more information.)
Page 22: Attaching The Reflux Head To The Reaction Station
Step 1 Position the Reflux Head on the mounting support tower, which is on top of the Mya 4 unit, so that the horizontal tab at the rear of the Reflux Head is aligned with the horizontal slot in the support tower.
Page 23  As the Reflux Head is heavy, you should ensure it is fully supported at all times during the location process. Avoid excessive contact with the surface of the Mya 4 support tower as this may scratch it.  Once the tab on the Reflux Head is located in the support tower slot, the weight of the Reflux Head will be fully supported, but it is advisable that you continue to manually support the front of the Reflux Head until both securing bolts are partially tightened, to avoid the head slipping and damaging the Mya 4 main unit.
Page 24: Connecting The Coolant Supply To The Reflux Head
For best performance, it is recommended that a separate coolant supply is used for both the Mya 4 and the Reflux Head to allow independent control of the coolant flow rate and temperature.
Page 25 Mya 4 Reaction Station – Instructions Step 1 Attach a quick-release male connector (RR95525) to the end of the coolant tubing from your coolant source, securing with a tubing clamp (RR40634). Note We recommend reinforced coolant tubing RR40627. Step 2...
Page 26: Connecting A Single Coolant Supply To The Reaction Station And Reflux Head
RR40628 Notes → To ensure correct operation of Mya 4, it is important to control and monitor the coolant flow within the stated operating limits at all times by using the coolant flowmeter (RR40633) supplied. → All tubing should be fully secured using a tubing clamp (RR40634). Slide the tubing clamp over the end of the tubing before connecting the tubing to either a quick-release connector or other component, such as a flowmeter or Y-piece.
Page 27 (RR40828) from the Coolant In and Out female connectors at the back of the Reaction Station. Note Keep the blanking plugs safe as you may need to reinsert them if moving Mya 4 to a different location. (See Notes below.)
Page 28 Mya 4 Reaction Station – Instructions Step 5 Connect another length of coolant tubing to the upper fitting of the coolant flowmeter, securing with a tubing clamp. Attach the quick-release male connector (RR95525) to the other end of this tubing, securing with a tubing clamp.
Page 29 Mya 4 Reaction Station – Instructions Step 9 Connect a length of coolant tubing To Reaction To Reflux from the coolant return (drain or Station coolant Head coolant outlet outlet recirculator) to the single end of a Y- piece tubing connector (RR40628), securing with a tubing clamp.
Page 30 However, the female connectors at the rear of the Reaction Station do not have shut-off valves. If you need to move Mya 4 to a different location, use the blanking plugs (see Step 1 above) to block these connectors off during transportation.
Page 31 Mya 4 Reaction Station – Instructions Notes → To ensure correct operation of Mya 4, it is important to control and monitor the coolant flow within the stated operating limits at all times by using the coolant flowmeter (RR40633) supplied.
Page 32 Then insert one end of this tubing into the Coolant Out outlet at the rear of Mya 4, and the other to the Water In inlet at the rear of the Reflux Head. Step 6 Attach a male connector to another length of coolant tubing.
Page 33: Connecting The Gas/Vacuum Supply To The Reflux Head
Mya 4 Reaction Station – Instructions 6.7.4 Connecting the gas/vacuum supply to the Reflux Head The gas inlet/outlet and distribution system combines with the Easy-On PTFE caps or Mya Compact Stirrers to allow reactions to be performed under an inert (i.e. nitrogen or argon) atmosphere.
Page 34 Vessel contents under vacuum • A continuous vacuum can only be applied to vessel contents on Mya 4 as long as the vacuum is not pulled through the Mya 4 Reflux or Manifold Head.
Page 35 Notes → Unless otherwise stated, Mya 4 reaction vessels are suitable for use under vacuum, typically down to 10 mbar, but not for use under positive pressure. → The vacuum level that is obtained in a set-up will depend on various factors, including: •...
Page 36: Important Tips For Optimum Refluxing
 Dismantling the head will compromise the silicone seal between the surfaces and cause a water leak during operation.  In the unlikely event of a water leak from the Reflux/Manifold Head, please stop using the unit immediately. • Switch off the power supply to Mya 4 at the mains. • Remove the power plug from the mains.
Page 37: Attaching The Manifold Head To The Reaction Station
Step 1 Position the Manifold Head on the mounting support tower at the top of the Mya 4 unit, so that the horizontal tab at the rear of the Manifold Head is aligned with the horizontal slot in the support tower.
Page 38  As the Manifold Head is heavy, you should ensure it is fully supported at all times during the location process. Avoid excessive contact with the surface of the Mya 4 support tower as this may scratch it.  Once the tab on the Manifold Head is located in the support tower slot, the weight of the Manifold Head will be fully supported, but it is advisable that you continue to manually support the front of the Manifold Head until both securing bolts are partially tightened, to avoid the head slipping and damaging the Mya 4 main unit.
Page 39: Connecting The Coolant Supply To The Manifold Head
For best performance, it is recommended that a separate coolant supply is used for both the Mya 4 and the Manifold Head to allow independent control of the coolant flow rate and temperature.
Page 40 Mya 4 Reaction Station – Instructions Step 1 Attach a quick-release male connector (RR95525) to the end of the coolant tubing from your coolant source, securing with a tubing clamp (RR40634). Note We recommend reinforced coolant tubing RR40627. Step 2...
Page 41: Connecting A Single Coolant Supply To The Reaction Station And Manifold Head
RR40628 Notes → To ensure correct operation of Mya 4, it is important to control and monitor the coolant flow within the stated operating limits at all times by using the coolant flowmeter (RR40633) supplied. → All tubing should be fully secured using a tubing clamp (RR40634). Slide the tubing clamp over the end of the tubing before connecting the tubing to either a quick-release connector or other component, such as a flowmeter or Y-piece.
Page 42 (RR40828) from the Coolant In and Out female connectors at the back of the Reaction Station. Note Keep the blanking plugs safe as you may need to reinsert them if moving Mya 4 to a different location. (See Notes below.)
Page 43 Mya 4 Reaction Station – Instructions Step 5 Connect another length of coolant tubing to the upper fitting of the coolant flowmeter, securing with a tubing clamp. Attach the quick-release male connector (RR95525) to the other end of this tubing, securing with a tubing clamp.
Page 44 Mya 4 Reaction Station – Instructions Step 9 Connect a length of coolant tubing To Reaction To Manifold from the coolant return (drain or Station coolant Head coolant outlet outlet recirculator) to the single end of a Y- piece tubing connector (RR40628), securing with a tubing clamp.
Page 45 However, the female connectors at the rear of the Reaction Station do not have shut-off valves. If you need to move Mya 4 to a different location, use the blanking plugs (see Step 1 above) to block these connectors off during transportation.
Page 46 Mya 4 Reaction Station – Instructions Notes → To ensure correct operation of Mya 4, it is important to control and monitor the coolant flow within the stated operating limits at all times by using the coolant flowmeter (RR40633) supplied.
Page 47 Then insert one end of this tubing into the Coolant Out outlet at the rear of Mya 4, and the other to the Water In inlet at the rear of the Manifold Head. Step 6 Attach a male connector to another length of coolant tubing.
Page 48 Mya 4 Reaction Station – Instructions When connecting the main unit and Manifold Head in series, either a glass coil condenser (RR40556) or Manifold Head bypass (RR40817 – supplied with the Manifold Head) must be attached for water to flow through the unit.
Page 49: Connecting The Gas/Vacuum Supply To The Manifold Head
Mya 4 Reaction Station – Instructions 6.7.9 Connecting the gas/vacuum supply to the Manifold Head The gas inlet/outlet and distribution system combines with the Easy-On PTFE caps or Mya Compact Stirrers to allow reactions to be performed under an inert (i.e. nitrogen or argon) atmosphere.
Page 50  Mya 4 Reflux and Manifold Heads are not suitable for continuous use under vacuum (e.g. for solvent evaporation or reactions under vacuum), as this may lead to contamination or corrosion inside the head. Vacuum should only be used intermittently as part of a gas purge cycle.
Page 51: Attaching Glass Condensers To The Manifold Head
Mya 4 Reaction Station – Instructions 6.7.10 Attaching glass condensers to the Manifold Head To attach glass coil condensers (RR40556) to the Manifold Head, you will need these components: RR40816 RR40556 Manifold Head to glass condenser Coil Condenser for Mya tubing &...
Page 52 Mya 4 Reaction Station – Instructions Step 3 Take another length of tubing + connector. Attach the GL14 end of the tubing to the top connector on the Condenser. Attach the quick-release male connector on the other end of the tubing to the right female connector on the Manifold Head (Water out).
Page 53: Important Tips For Optimum Refluxing
 Dismantling the head will compromise the silicone seal between the surfaces and cause a water leak during operation.  In the unlikely event of a water leak from the Reflux/Manifold Head, please stop using the unit immediately. • Switch off the power supply to Mya 4 at the mains. • Remove the power plug from the mains.
Page 54: Stirring Options
Stirring options If you require stirring of your samples, you have two options: Magnetic stirring Mya 4’s integrated magnetic stirring with magnetic stirring bars. Overhead stirring A Mya Compact Stirrer and a choice of stirrer shafts (purchased separately). A Mya Compact Stirrer...
Page 55: Magnetic Stirring
100 and 1000 rpm. When activated, stirring will smoothly ramp up to the set speed. Stirring is set, controlled and recorded for an experiment via the Mya 4 Control Pad. (See Section 7 (page 79) for more information about the Control Pad.) Once you have selected your glassware (see page 56), choose a magnetic stirring bar and place in the reaction vessel.
Page 56: Mya 4 Reaction Vessel Options
Mya 4 Reaction Station – Instructions Mya 4 reaction vessel options A wide range of reaction vessels are available for use with Mya 4. (See Section 12 on page 162 for more information.) Sizes vary from 2 ml to 400 ml, and the range includes vials, tubes, round bottom flasks (RBFs) and process vessels.
Page 57: Mya 4 Inserts
• 4 to fit RBFs, • 4 to fit process vessels. Every reaction vessel from the Mya 4 range will require an appropriate insert, apart from the 400 ml process vessels. These fit directly into the zones in the Reaction Station.
Page 58 Mya 4 Reaction Station – Instructions Inserts for vials and tubes Inserts for vials and tubes are single or multi-position. Multi-position inserts have a temperature probe hole. RR40420 Mya Insert for 8 x 12 mm Vials x 14 mm Deep...
Page 59: Reflux Tubes And Multi-Neck Lids
Mya 4 Reaction Station – Instructions 6.11 Reflux tubes and multi-neck lids In order to connect round bottom flasks (RBFs) and process vessels to the Reflux or Manifold Head, you will need a reflux tube or multi-neck lid. Reflux tubes (standard and wide-neck) can be used with both the Reflux and Manifold Heads, and allow attachment to the inert gas supply through the Easy-On cap.
Page 60: Assembling Glassware And A Reflux Tube
Mya 4 Reaction Station – Instructions 6.11.1 Assembling glassware and a reflux tube If you are using glassware with the Reflux Head, you will first need to attach a standard or wide-neck reflux tube to the glassware. The type of reflux tube you need will depend on what glassware you are using: •...
Page 61 Mya 4 Reaction Station – Instructions Standard RBF If you are using a standard RBF, you will need to attach a standard reflux tube (RR99918/1) with a ® Rodaviss ground glass joint. Notes → Rodaviss is an extra-safe borosilicate glass joint for connecting laboratory glassware. Rodaviss is safe under vacuum or pressure, grease-free, interchangeable with standard 1:10 tapered ground joints (including A and B lengths), will not stick or jam, is extremely strong and can be used up to 200 ˚C.
Page 62 Mya 4 Reaction Station – Instructions Step 3 Clip the black loosening ring between the top of the connecting cap and glass rim on the cone. Step 4 Now insert the cone of the reflux tube into the neck of the flask. Screw down...
Page 63 Mya 4 Reaction Station – Instructions Wide-neck glassware If you are using a wide-neck round bottom flasks (RBF) or process vessel, you will need to attach a wide-neck reflux tube (RR99924). Step 1 Fit the nitrile O-ring around the support collar.
Page 64 Mya 4 Reaction Station – Instructions Step 4 Place the adjoined support collar and O-ring onto the flat flange of the flask. Step 5 Place the reflux tube on top of the flask and screw the upper and lower couplings together.
Page 65: Attaching An Easy-On Cap To A Reflux Tube
Mya 4 Reaction Station – Instructions 6.11.2 Attaching an Easy-On cap to a reflux tube • Assemble the reflux tube and Easy-On cap by screwing together. • This process requires a ¼ turn of the cap; hand tighten until the cap feels secure.
Page 66: Assembling A Reaction Vessel And A Multi-Neck Lid
Mya 4 Reaction Station – Instructions 6.11.3 Assembling a reaction vessel and a multi-neck lid Step 1 Fit the nitrile O-ring around the support collar. Step 2 Prise apart the open edges of the upper coupling and place around the neck of the multi-neck lid.
Page 67 Mya 4 Reaction Station – Instructions Step 5 Place the adjoined support collar and O-ring onto the flat flange of the flask. Screw the middle coupling onto the thread of the lower coupling. Step 6 Place the multi-neck lid onto the flask assembly.
Page 68: Using Overhead Stirrers
Mya 4 Reaction Station – Instructions 6.12 Using overhead stirrers Mya Compact Stirrers can be used with wide-neck RBFs or process vessels, together with either a wide-neck reflux tube or a multi-neck lid. You will need to attach a stirrer shaft to the Mya Compact Stirrer. A wide range of Mya stirrer shafts are available.
Page 69: Assembling The Mya Compact Stirrer
Mya 4 Reaction Station – Instructions 6.12.1 Assembling the Mya Compact Stirrer Mya Compact Stirrer Locking nut Cable Coupling Locking collet Mya Stirrer Guide – PEEK Bearing Cable connector Sub-Assembly Mya Stirrer Guide – PTFE Cap Sub-Assembly Step 1 Attach the Mya Stirrer Guide to your chosen reflux tube or multi-neck lid using the quick-thread adapter.
Page 70 Mya 4 Reaction Station – Instructions Step 3 Feed the stirrer shaft through the underside of the lid into the guide at the bottom of the PTFE Cap until it protrudes through the locking collet. Note The rounded top section of the...
Page 71 Mya 4 Reaction Station – Instructions Step 7 Now engage the PTFE Cap with the Reflux/Manifold Head, ensuring that it is correctly located on the inert gas supply nozzle. Step 8 Lower the Compact Stirrer onto the PTFE Cap until the locking nut engages with the thread on the head.
Page 72: Stirrer Guide Care
6.12.2 Stirrer guide care The Mya stirrer guide is a precision-engineered component of your Mya 4 that allows proper connectivity, sealing, and alignment of the Mya Compact Stirrer, stirrer shaft, and Mya 4 glassware. Always handle the Mya Stirrer Guide – PEEK Bearing Sub-Assembly with great care to avoid damage to the sealing surfaces and features.
Page 73 Mya 4 Reaction Station – Instructions Step 1 If the sealing edge is misshapen, carefully reshape using a fingernail or soft instrument (e.g. pen cap). Step 2 Ensure the seal profile resembles a circular shape and is not cracked. Note If the seal is cracked, please contact Radleys or your local supplier.
Page 74 Mya 4 Reaction Station – Instructions Step 5 Ensure the tool does not fall from the stirrer guide in vertical position. Note If the tool will not hold by friction alone, please contact Radleys or your local supplier. Roll the O-ring on the tool until it located into the sealing edge on the stirrer guide.
Page 75: Using External Temperature Probes
(Tb) at all times when the reaction station is powered on. Mya 4 features optional external Pt100 temperature probes that you can connect to each zone of the Reaction Station. The external Pt100 provides additional temperature monitoring and control of either the reactor contents, or the aluminium insert if tubes or vials are being used.
Page 76: Connecting An External Temperature Probe
Mya 4 Reaction Station – Instructions 6.13.1 Connecting an external temperature probe Plug the probe connector into the probe socket on the connector panel, at the top of the support tower. Probe connector Use the alignment tab on the probe connector to ensure that the holes in the probe socket line up with the pins of the probe connector.
Page 77 Mya 4 Reaction Station – Instructions Through a sidearm When securing a probe through the sidearm of a vessel, use a temperature probe adapter to secure the probe. (See picture 1 below.) You may need a long probe (225 mm). Alternatively, push the probe through the septum in the sidearm (cut a small hole first).
Page 78 Mya 4 Reaction Station – Instructions In the probe hole of a multi-vial insert When using a probe with multi-vial inserts, rotate the insert so that the probe hole is accessible, then push the probe into the probe hole. It is recommended that you use a probe with a stainless steel finish, as the coating on a PTFE-coated probe could become damaged when moving it in and out of inserts.
Page 79: The Mya 4 Control Pad
Mya 4 Reaction Station – Instructions The Mya 4 Control Pad The Mya 4 Reaction Station can be controlled with the Mya 4 Control Pad. Touch-screen interface Ethernet port USB port The Control Pad allows you to set up and control experiments in Manual or Profile mode.
Page 80: Set-Up And Operation
Warning  Never connect the Mya 4 Control Pad to a laptop or PC as this may result in serious damage to your device.  The Mya 4 Control Pad screen is made of chemically resistant glass. However, any chemical or liquid spills on the screen should be cleaned up immediately.
Page 81: Using The Control Pad
• You may prefer to use a capacitive stylus (RR40823, supplied with Mya 4). In this case, lightly touch the screen with the capacitive end of the stylus on the required point/icon to get a response.
Page 82: Overview Screen
Status icon • Tap the Overview icon in the side panel for an overview of all four zones of the Mya 4 unit. • From here, you can access three different overviews – Apparatus, Profile or Graph – via the Overview Navigation tabs.
Page 83: Apparatus
Mya 4 Reaction Station – Instructions 7.3.1 Apparatus The Apparatus Overview screen displays the current set-up for all four zones. Experiment name and run mode Zone Navigation icon Overhead External Stirrer icon Temperature Probe icon and Reaction Temperature box Magnetic...
Page 84 Notes → In the unlikely event that the Control Pad is unable to communicate with the Mya 4 Reaction Station, the Control Pad will switch to demo mode and the System Status icon will read DEMO. To switch back to READY mode, go to System Settings (see page 122) and switch Demo mode off.
Page 85: Profile
Mya 4 Reaction Station – Instructions 7.3.2 Profile The Profile Overview screen displays a timeline for each zone where a profile for an experiment has been set. For zones where no profile is set, i.e. if zones are running in Manual mode, then no profile is displayed in this view.
Page 86: Graph
Mya 4 Reaction Station – Instructions 7.3.3 Graph The Graph Overview screen displays a graph for each zone where an experiment is or has been running. The graph will continuously update as the experiment progresses. No graph is displayed for zones where an experiment hasn’t been created, or the experiment hasn’t started.
Page 87: Zone View Screens
Mya 4 Reaction Station – Instructions Zone View screens Each of the four Mya 4 zones has its own individual screen. From an individual Zone View screen, you can: • create and run a new experiment (see page 104 for information on how to create a new experiment), •...
Page 88: Manual Mode
Mya 4 Reaction Station – Instructions 7.4.1 Manual mode In Manual mode, temperatures and stirrer speeds are manually entered and can be changed at any time by the user. Experiment name Reaction Temperature box Block Temperature box Temperature Temperature Control On/Off...
Page 89 Mya 4 Reaction Station – Instructions Block and Reaction Temperature boxes • When Tb is selected in Temperature Control mode, the Block Temperature box will display an orange highlight. • When Tr is selected in Temperature Control mode, the Reaction Temperature box will display an orange highlight.
Page 90 Mya 4 Reaction Station – Instructions Stirrer Speed Setting box • Tap once on the Stirrer Speed Setting box to set the stirring speed of the experiment. • Enter the required stirrer speed using the pop-up keypad. You can enter any value between 100 rpm and the maximum safety limit applied in Settings (see page 100).
Page 91: Profile Mode
Mya 4 Reaction Station – Instructions 7.4.2 Profile mode In Profile mode, a predefined experiment can be created and then run in a fully automated way. With the Profile Set-up tab selected, you can create a sequence of steps in the Profile table (up to a maximum of 10 for each experiment), each with its own independent values.
Page 92 Mya 4 Reaction Station – Instructions Profile step • With the Profile Set-up tab selected, the Profile table will display a default first Profile step. Tap once anywhere on the default step to select it. It will display an orange highlight.
Page 93 Mya 4 Reaction Station – Instructions Temperature Control mode To control the temperature of each step of the experiment, you can: • use the internal Pt100 probe, which controls the block temperature (tap Tb once), • or, place an additional external temperature probe directly in the reaction vessel, or in a multi- vial insert, to control the reaction temperature (tap Tr once).
Page 94 Mya 4 Reaction Station – Instructions Temperature Setting boxes • Tap once on the From Temperature Setting box to set the start temperature for a step. Enter the required value on the pop-up keypad and select Apply. • Then tap once on the To Temperature Setting box to set the end temperature for a step. Again, enter the required value on the pop-up keypad and select Apply.
Page 95 Notes → If the start and end temperatures for a step are different, Mya 4 will calculate a linear gradient between the two temperatures over the set duration, and will then track this gradient as closely as possible. End Condition box Tap once on either Value or Time to set the end condition of the step.
Page 96 Mya 4 Reaction Station – Instructions Timeline • There is a timeline under the Profile table, which is segmented to show the details of each individual profile step set for an experiment. • The yellow segments represent the temperature and the blue segments represent the stirrer speed.
Page 97: Using Graphs In Zone View
Mya 4 Reaction Station – Instructions 7.4.3 Using graphs in Zone View • In Manual mode: When you view the set-up for an individual experiment in Manual mode, the graph for that experiment is displayed on the same screen. • In Profile mode: To view the graph for an experiment in Profile mode, tap once on the Graph navigation tab.
Page 98 Mya 4 Reaction Station – Instructions Once an experiment starts, a new graph is created, and data plotting automatically begins. Each set of values that is plotted on the graph is represented by a different-coloured line as follows: Actual block temperature (Tb)
Page 99 Mya 4 Reaction Station – Instructions Graph Zoom function (expanded view) To zoom in on a specific part of the graph, double tap on the area of interest. • You can zoom in further by continually double tapping the area of interest.
Page 100: Zone Settings
→ You cannot change the Temperature Control option or safety limits values once an experiment has started. Temperature Control options Mya 4 uses bespoke PID algorithms to facilitate the control of both the block (Tb) and reaction (Tr) temperatures, ensuring best performance (minimum overshoot and minimum fluctuation) in a range of different scenarios.
Page 101 Mya 4 Reaction Station – Instructions OPTION A This is the default option for all experiments. It gives the best performance over the whole operating temperature range of Mya 4, but, in some circumstances, other options may give a better performance. OPTION B This option will give the best performance at moderate temperatures (e.g.
Page 102 Mya 4 Reaction Station – Instructions Safety Limits panel • You can set independent safety limits for each zone. To do this, tap once on any of the boxes in the grey Safety Limits panel and enter the value on the pop-up keypad.
Page 103 Mya 4 Reaction Station – Instructions • You can set a maximum stirrer speed of between 100 and 1000 rpm. • You can set the minimum (Min Tb) and maximum (Max Tb) block temperatures between -30 and 180 °C, but you cannot enter a Max Tb value that is lower than the Min Tb value or a Min Tb value that is higher than the Max Tb value.
Page 104: File Management
Mya 4 Reaction Station – Instructions File Management To manage experiment files for each individual zone, use the File Management icons in the Zone View. For general file management (for all zones), use the System File Manager (folder icon) on the side panel.
Page 105 Mya 4 Reaction Station – Instructions New experiment • Tap this icon once to create a new experiment. • A default experiment name will be displayed on the pop-up keypad. • You can overwrite the default name by typing into the pop-up keypad.
Page 106 Mya 4 Reaction Station – Instructions Open file • Tap once on this icon to open up a list of previously saved experiments. • Use the arrows in the scroll bar on the right to move up and down the list. Experiments are listed alphabetically and numerically.
Page 107 • An asterisk next to the experiment name indicates that some changes you’ve made to the experiment values haven’t been saved yet. • If the Mya 4 unit is switched off with the asterisk still displayed, any unsaved changes will be lost. Unsaved changes Notes →...
Page 108 Mya 4 Reaction Station – Instructions Save experiment as • Tap once on this icon to save the currently displayed experiment as a new experiment (with all the same settings) that can be run again. • You will be prompted to enter a new name for the duplicated experiment on the pop-up keypad (or you can use the default name provided) and to save it.
Page 109: General File Management (All Zones)
Mya 4 Reaction Station – Instructions 7.5.2 General file management (all zones) Tap once on the System File Manager icon in the side panel to access the File Manager screen. (The System File Manager icon will display an orange highlight when selected.)
Page 110 Mya 4 Reaction Station – Instructions Loading an experiment file to one of the four zones With the selected experiment highlighted in orange, you can tap on a File Transfer arrow to transfer the experiment to one of the four corresponding zones. Once the experiment has loaded to a zone, you can either view the experiment data or edit/rerun the experiment from the Zone View screen (tap on the numbered Zone Navigation icon in the side panel to access the zone).
Page 111 Mya 4 Reaction Station – Instructions You cannot transfer an experiment into a zone if that experiment is already loaded in that zone. In the example below, Experiment 34 has been selected, but as it is already loaded in Zone 4, the transfer arrow to that zone is disabled.
Page 112 → An expc file, which cannot be opened on a PC. This file contains all the settings associated with the experiment, and can be transferred back onto the original Mya 4 Control Pad, or any other Mya 4 Control Pad. Using this file allows experiments to be transferred from one Mya 4 unit to another.
Page 113 Failure to allow enough time may result in incomplete data transfer. → Once the USB device is removed from the Mya 4 Control Pad, the USB transfer area will still temporarily show the transferred experiment file(s). To clear the transfer area, select the Share tab.
Page 114 • Connect your PC/laptop to your network and type in the configured IP address in a file explorer window in the format \\IPaddress\Share (e.g. \\192.168.1.10\Share). • The Mya 4 Share area with the loaded files should be displayed in the file explorer. See notes page 112.
Page 115 Stored Experiments list. Notes → If you simply want to transfer experiments from one Mya 4 unit to another, you only need to import the .expc experiment file, which contains the experiment settings. → If you also require the data from an experiment, for example, to create graphs in a program such as Microsoft Excel, then you should also import the associated .csv file.
Page 116 Mya 4 Reaction Station – Instructions Delete experiment files • Tap once on the experiment file you want to delete in the Stored Experiments list (the experiment will display an orange highlight). • Now tap the Delete icon and this permanently deletes the selected file.
Page 117 Mya 4 Reaction Station – Instructions Duplicate experiment files You can create the exact same experiment in multiple zones. In Manual mode, you will need to access each zone individually and enter the same values in each zone. In Profile mode, you can create a profile for an experiment in one zone and then copy it to any of the other zones using the Open File icon in each zone, or the System File Manager.
Page 118 Mya 4 Reaction Station – Instructions Step 3 • Select the master experiment (it will display an orange highlight) from the list and then tap Open. • Type in a new name for the experiment using the pop-up key pad, and tap Save. In the example below, the new experiment is called template exp z2 and has been saved to Zone 2.
Page 119 Mya 4 Reaction Station – Instructions With the System File Manager Step 1 • Create your master experiment in Profile mode and save it. Step 2 • Tap on the System File Manager icon in the side panel (it will display an orange highlight).
Page 120 Mya 4 Reaction Station – Instructions Step 4 • You will be prompted to type in a new name for the experiment using the pop-up key pad (or you can use the default name provided). • Now tap Save. • The same experiment profile from Zone 1, together with all the safety settings, will now be loaded into the new zone under the new experiment name.
Page 121 Mya 4 Reaction Station – Instructions Create a screenshot You can create and export a screenshot of the currently displayed screen at any time. To do this: • Insert a USB storage device into the USB port on the Control Pad.
Page 122: System Settings
Mya 4 Reaction Station – Instructions System Settings Tap once on the System Settings navigation icon to go to the Settings screen, where there are four Settings tabs: • System Settings • Service Settings • PID Gains • Network Share...
Page 123 Mya 4 Reaction Station – Instructions Notes → The daily password is generated using the date set in the internal clock (see page 125). If the password entered fails to open the required tab, check that the date is correctly set.
Page 124: System Settings Tab
Mya 4 Reaction Station – Instructions 7.6.1 System settings tab Data Storage Interval • When an experiment is running, data is reported and plotted on the graph every two seconds. This value cannot be changed. • The Data Storage Interval box displays how often this reported data is actually stored with the experiment file.
Page 125 • The default Demo Mode setting is Off. • Tapping On in the Demo Mode box will set the Mya 4 Control Pad to Demo mode. This allows the Control Pad to simulate an experiment without actually activating any temperature and stirrer controls on the Mya 4 unit.
Page 126: Service Settings Tab
• Tap once on Export Controller Settings. • A folder named Mya 4 Controller Export will be created on the external USB storage device, containing: an XML document listing all system settings, experiment data for each experiment currently loaded in the four zones.
Page 127 Notes → The Error Log will only be available for export when there are no experiments running on Mya 4. You will need to stop running any experiments (or wait for them to be completed) in order to export the Error Log (e.g. to send it to Technical Support).
Page 128: Pid Gains Tab
122 for more information on password protected tabs.) • Mya 4 is supplied with four pre-set Temperature Control options: A, B, C and D (see page 135).These Control options have been optimised to cover most general applications, and are displayed on the PID Gains screen.
Page 129: Network Share Tab
Mya 4 Reaction Station – Instructions 7.6.4 Network Share tab The Network Share tab allows the configuration of the Share area in File Manager. To access the Share area on a network via an Ethernet connection, the Control Pad must have a valid IP address for the network.
Page 130: Safety
Notes → Mya 4 features an internal gas purge to further protect internal components. The purge inlet must be connected to a suitable dry gas supply (nitrogen, argon or compressed air), which is switched on at all times during operation.
Page 131: Electrical Safety
EN61 010–1, American equivalent UL 61010–1 and Canadian equivalent IEC 61010-1. Notes → If a thermal fuse trips in any zone, that zone will no longer function and Mya 4 will need to be repaired by Radleys or a Radleys authorised service engineer.
Page 132: Coolant Temperature (Tc) Sensors
Mya 4 Reaction Station – Instructions temperature at all times when the Reaction Station is powered on. These sensors cannot be accessed by the user. • The minimum (Default Min Tb) and maximum (Default Max Tb) operating temperatures for these sensors are pre-set by the manufacturer. These are the values shown in brackets in the Safety Limits panel for each zone (under the Settings tab –...
Page 133: Reaction Temperature (Tr) Sensors
Safety Limits panel, you will not be able to enter values that fall outside the manufacturer’s pre- set range. • If a reaction temperature is being measured by a Pt100 connected to Mya 4, and it exceeds the manufacturer’s pre-set limits, the corresponding zone will revert to a safe state. (See Error Codes on page 156.)
Page 134: Max Temperature Difference (Tb-Tr)
8.3.5 Max temperature difference (Tb–Tr) Mya 4 features additional thermal safety measures for when the external temperature probe is connected. The difference between the Tb (block temperature) and Tr (reaction temperature) is constantly calculated, and limited by default to 50 °C.
Page 135: Temperature Control
Mya 4 Reaction Station – Instructions Temperature control Mya 4 uses bespoke PID algorithms to facilitate the control of both the block (Tb) and reaction (Tr) temperatures, ensuring best performance (minimum overshoot and minimum fluctuation) in a range of different scenarios.
Page 136 As the PID values for all the pre-set Temperature Control options are identical for Tb control (optimised settings for Mya 4), if your experiment is being controlled by block temperature, all options (A, B, C and D) will give the same rapid and accurate response.
Page 137 To see examples of the effect of different Temperature Control options, see page 139. In Manual mode, Mya 4 will achieve the set temperature in the quickest time possible, dependent only on the Control option (A, B, C or D) selected.
Page 138 Mya 4 Reaction Station – Instructions box (to ensure the set temperature is reached, even if this takes longer than the time set for the step in the Duration box). Example of creating a single setpoint profile step to reach the set temperature as quickly as possible In the example below, the current reaction temperature (Tr) is 19.9 °C and a profile step has been...
Page 139 Mya 4 Reaction Station – Instructions Examples of effects of Temperature Control PID option selection The set-up for examples 1 to 4 shown below is as follows: • a 250 ml round bottom flask, • 120 ml reaction contents, • control by reaction temperature with an external probe directly in the reaction mixture.
Page 140 Mya 4 Reaction Station – Instructions Example 2 Single setpoint profile step, cooling from 155 °C to 135 °C 155 to 135 °C, 4 PID options Time (min) Tr Set Tr Option A Tr Option B Tr Option C Tr Option D Options A and B both overshoot by around 8 °C.
Page 141 Mya 4 Reaction Station – Instructions Example 3 Single setpoint profile step, cooling from 30 °C to 0 °C 30 to 0 °C, 4 PID options Tr Set Tr Option A Tr Option B Tr Option C Tr Option D Option A produces an overshoot of about 5 °C.
Page 142 Mya 4 Reaction Station – Instructions Example 4 Single setpoint profile step, heating from -15 °C to -5 °C -15 to -5 °C, 4 PID options Time (min) Tr Set Tr Option A Tr Option B Tr Option C Tr Option D Options A and B overshoot by 4 °C and 5 °C respectively.
Page 143: Creating A Ramped Profile Step
Mya 4 Reaction Station – Instructions 9.1.2 Creating a ramped profile step When heating/cooling as fast as possible, overshoots are to be expected. So if temperature control to minimise overshoots is critical, creating a ramped profile step for each step is the recommended method.
Page 144 Mya 4 Reaction Station – Instructions Examples of temperature control obtained using ramped profile steps The set-up for examples 5 and 6 shown below is as follows: • a 250 ml round bottom flask, • 120 ml reaction contents, • control by reaction temperature with an external probe directly in the reaction mixture, •...
Page 145 Mya 4 Reaction Station – Instructions Example 6 Heating from -15 °C to -5 °C, comparing a single setpoint profile step with various ramped profile steps, using Option A. -15 to -5°C, Single setpoint vs various ramps Time (min) Tr Set single setpoint...
Page 146: Adjusting The Max Temperature Difference: (Tb-Tr)
Mya 4 Reaction Station – Instructions 9.1.3 Adjusting the Max Temperature Difference: (Tb–Tr) Another way to optimise temperature control is by adjusting (Tb–Tr) (the maximum temperature difference between the block and reaction mixture, also referred to as delta T max) for a given experiment.
Page 147 Mya 4 Reaction Station – Instructions Example of the effect on temperature control of adjusting (Tb–Tr) The set-up for the example shown below is as follows: • a 250 ml round bottom flask, • 120 ml reaction contents, • control by reaction temperature with an external probe directly in the reaction mixture, •...
Page 148: Configuring Your Own Pid Values
Mya 4 Reaction Station – Instructions Configuring your own PID values The four pre-set Temperature Control options should be suitable for most applications. However, in extreme cases, it may be necessary to optimise beyond what the pre-set options can achieve for a given application.
Page 149 Mya 4 Reaction Station – Instructions The PID Gains screen will now be displayed. For each option, there is a set of PID values for control of the block, and a set of PID values for control of the reaction. Both the currently set values (shown in the white boxes) and the default values (shown underneath the white boxes) are displayed.
Page 150 Mya 4 Reaction Station – Instructions Notes → As a guideline, when attempting to optimise the PID values, begin by adjusting just the ‘P’ value by a small amount, up or down. Then run your experiment, and observe the effect.
Page 151: Error Handling
Mya 4 features built-in error detection to allow safe operation of the equipment at all times. If an error is detected, it will be reported on the Mya 4 Control Pad, along with relevant information about the error, and instructions on how to continue.
Page 152 Mya 4 Reaction Station – Instructions In Zone view, tap once on the yellow warning icon to display the Error Message box. The Error Message box shows the error location, error description, safe state status and troubleshooting instructions. If more than one error has been detected, you can scroll between different Error Message boxes using the arrows.
Page 153: Red Zone Error
Mya 4 Reaction Station – Instructions 10.2 Red Zone error When a Red Zone error occurs, the flask navigation icon for the affected zone will be red, and a red warning icon is displayed. For example, a Red Zone error in Zone 2 would look like this:...
Page 154 Mya 4 Reaction Station – Instructions A red zone error will turn off active temperature control and stirring in the affected zone. Notes → A Red Zone error only acts on the zone where the error occurred; other zones will continue operating normally.
Page 155: Red System Error
Mya 4 Reaction Station – Instructions 10.3 Red System error A Red System error affects all zones and will force the Apparatus overview screen to be displayed. All flask icons will be red, and a red warning icon will be in the side navigation panel.
Page 156: Error Codes
Mya 4 Reaction Station – Instructions 10.4 Error codes RZ = Red Zone YZ = Yellow Zone RS = Red System Error Error reported Likely cause Resolution type Magnetic stirrer has exceeded Jammed or uneven operation of stirrer Check stirrer is free to rotate...
Page 157 Mya 4 Reaction Station – Instructions PCB may need replacing 27V supply fault Fault on system control PCB Contact supplier PCB may need replacing 3V3Q supply fault Fault on system control PCB Contact supplier PCB may need replacing EEPROM missing...
Page 158: Technical Specifications
Mya 4 Reaction Station – Instructions Technical specifications Power supply Voltage 100–240 V AC Frequency 50/60 Hz Power consumption 1.5 kW (max) Fuse rating 15 A Operating conditions Ambient temperature +10 to +30 °C Relative humidity 10–80% Temperature Block (with coolant at 5 °C & 2 L/min) -30 to 180 °C...
Page 159 Mya 4 Reaction Station – Instructions Cooling of the instrument Coolant supply Mains water or ethylene glycol mix (max 30%) Max coolant temperature 35 °C Min coolant temperature 15 °C (up to 35 °C, however performance will be affected) Max flow rate...
Page 160 Mya 4 Reaction Station – Instructions Materials of construction Reaction Station Top cover Epoxy painted aluminium Lower chassis Epoxy painted steel Reaction blocks Anodised aluminium Gas feed nozzle 316 stainless steel with nitrile O-rings Coolant in/out connectors Acetal with internal 316 stainless steel & Buna seals...
Page 161 Mya 4 Reaction Station – Instructions Dimensions Reaction Station 600 (W) X 360 (D) X 370 (H) Top view Side view Control Pad Touch-screen dimensions: 95 (W) X 155 (H) Top view Side view Weights Mya 4 Reaction Station 33.2 kg Mya Control Pad 1.5 kg...
Page 162: Component Compatibility Guide
Mya 4 Reaction Station – Instructions Component compatibility guide Issue 8 Page: 162...
Page 163 Mya 4 Reaction Station – Instructions Issue 8 Page: 163...
Page 164 Mya 4 Reaction Station – Instructions Issue 8 Page: 164...
Page 165 Mya 4 Reaction Station – Instructions Issue 8 Page: 165...
Page 166 Mya 4 Reaction Station – Instructions Issue 8 Page: 166...
Page 167 Mya 4 Reaction Station – Instructions Issue 8 Page: 167...
Page 168 Mya 4 Reaction Station – Instructions Issue 8 Page: 168...
Page 169 Mya 4 Reaction Station – Instructions Issue 8 Page: 169...
Page 170 Equipment Description: Laboratory sample chiller, heater and stirrer. Equipment Cat No: RR40400 Manufacturer/Supplier: Radleys (R B Radley & Co Ltd) Address: Shire Hill, Saffron Walden, Essex, CB11 3AZ, UK Directives: EMC 2014/30/EU Electromagnetic Compatibility Directive LVD 2014/35/EU Low Voltage Directive...
Page 172 Shire Hill, Saffron Walden, Essex, CB11 3AZ, United Kingdom. t: +44 1799 513320 f: +44 1799 513283 © Radleys (R.B. Radley & Co Ltd), 2019 – (E&OE) e: sales@radleys.co.uk...

RADLEY Mya 4 Instructions Manual

1 Introduction

2 Mya 4 Standard Warranty

3 Safety Information

4 Important Warnings

5 Component Guide

6 Set-Up and Operation

7 The Mya 4 Control Pad

8 Safety

9 Temperature Control

10 Error Handling

11 Technical Specifications

12 Component Compatibility Guide

Quick Links

Need help?

Questions and answers

Summary of Contents for RADLEY Mya 4

Table of Contents