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DORIC Fiberless and Wireless Optogenetically Synchronized Electrophysiology System User Manual

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Fiberless and Wireless Optogenetically Synchronized
Electrophysiology System
User Manual
Version 2.1.0

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Summary of Contents for DORIC Fiberless and Wireless Optogenetically Synchronized Electrophysiology System

  • Page 1 Fiberless and Wireless Optogenetically Synchronized Electrophysiology System User Manual Version 2.1.0...

  • Page 2: Table Of Contents

    ............3 Doric Neuroscience Studio 3.1 Fiberless and Wireless Optogenetically Synchronized Electrophysiology...

  • Page 3: System Overview

    This allows small animal subjects high mobility while performing optogenetic and electrophys- iological recording experiments. The system can easily be integrated alongside other Doric Lenses products, including our Behavior Tracking Camera. A standard OSE system (Fig. 1.1) contains the following elements.
  • Page 4 • The Fi-Wi Opto-electric cannula. • The Electrophysiology Console. • The Dummy Headstage. • The USB Charger. • The Test Cannula. • The Implantation Holder. • The Behavior tracking camera (optional). 1.1.1 Fiberless & Wireless Headstage Figure 1.2: Fiberless & Wireless Headstage The Fiberless &...
  • Page 5 The Fi-Wi Opto-electric cannula (Fig. 1.3) contains an LED connected to a single optical fiber, allowing light to be sent into the region of interest. The cannula also contains the electrodes, positioned around the optical fiber, up to a maximum of 4.
  • Page 6 Figure 1.5: Electrophysiology Console, Rear 1.1.4 Fi-Wi Headstage Charger Figure 1.6: USB Charger The Fi-Wi Headstage Charger (Fig. 1.6) is used to recharge the headstage batteries and shut down the wireless connec- tion. During the pairing process, all unused headstages must be on the charger, as otherwise they could be detected instead of the one currently in use.
  • Page 7 1.1.6 Dummy Headstage The Dummy Headstage is a simplified version of the Fi-Wi Headstage that is used to habituate an animal subject to the weight of the headstage. It contains no electronics or other valuable components, and will not function as a Fi-Wi Head- stage.

  • Page 8: Operations Guide

    Operations Guide 2.1 System Setup The following steps assume the Fi-Wi Opto-electric Cannula is already implanted in the region-of-interest of the animal’s brain (Fig. 2.1). The process of implantation is not described in this document. Figure 2.1: Implanted Fi-Wi Opto-electric Cannula 2.1.1 Electrophysiology Console Setup Figure 2.2: Electrophysiology Console Setup 1.
  • Page 9 5. When the console is OFF, disconnect the USB-A/USB-B Cable before turning it back ON. Once the console is ON, reconnect the cable. 2.1.2 Software setup The following section details how to set up a measurement sequence using Doric Neuroscience Studio. Further detail for each software feature is found in section 3. Figure 2.3: Antenna Channel Configuration 1.
  • Page 10 (a) Channel configuration window setup (b) Acquisition setup Figure 2.4: Headstage startup elements 2.1.3 Fiberless & Wireless Headstage Setup The Fiberless and Wireless Headstage is the bridge between the Fi-Wi Opto-electric Cannula and the Electrophysiology Con- sole. Some preparation is required for optimal use. Figure 2.5: Headstage charger installation 1.
  • Page 11 Figure 2.6: Headstage placement 4. The headstage is connected to the cannula using a snap-on connector. For the two elements to connect properly, the headstage antenna ridge must be oriented towards the front of the cannula, as shown in Figure 2.6. 5.

  • Page 12: Doric Neuroscience Studio

    The Wireless OSE module incorporates different functions to control our Doric Fiberless & Wireless OSE system. The core of the system is the Fiberless & Wireless Headstage. This device controls a Wireless cannula containing several electrophysiological probes and an LED light source.
  • Page 13 The Trigger mode can be one of two types. In Triggered mode, the measurement sequence starts when a trigger signal is received, and continues even if the trigger signal stops. In Gated mode (currently in Chapter 3. Doric Neuroscience Studio...
  • Page 14 The Total Duration (Fig. 3.2) displays the total time of the experiment. The different values can be Inf for infinite, a valid time value or Err if the Time ON value is greater than 1/frequency. c) The Preview box shows a preview of the pulse sequence. Chapter 3. Doric Neuroscience Studio...
  • Page 15 Figure 3.4: Electrophysiology acquisition view, Antenna box 1. The Controls box (Fig. 3.5a) displays elements to control and monitor the wireless headstage. (a) Acquisition View, Controls (b) LED Trigger-Trigged by Acquisition Window Figure 3.5: Acquisition View Interfaces Chapter 3. Doric Neuroscience Studio...
  • Page 16 2. The Electrode Traces (Fig. 3.4) displays the signal detected by the cannula electrodes, in numbered order. 3. The LED Trace (Fig. 3.4) displays the current being sent to the cannula LED. Chapter 3. Doric Neuroscience Studio...

  • Page 17: Acquisition Console

    It enables control over the excitation light pulses, or the sinusoidal waveform trig of an ex- ternal source (i.e. Doric LED driver) with 4 TTL and 4 analog voltage outputs. The module interface displays real-time recordings of up to 4 input signals and performs basic signal processing. The system is controlled using 3 Control and Settings tabs.
  • Page 18 • In Gated mode, the sequence will play as long as there is a high TTL signal (4 V or more) on the input modulation BNC. This signal comes from a different light source or device driver. When the TTL signal is Chapter 3. Doric Neuroscience Studio...
  • Page 19 3. The Total Duration shows the total expected duration of the pulse sequence. Should the duration be infinite, the box will display . If there is an error in parameter selection, this box will display N/A. Chapter 3. Doric Neuroscience Studio...
  • Page 20 • The Input mode (Fig 3.7) receives a signal that is translated to 0 (Off) or 1 (On). The channel can then be used as a trigger source for all the other channels of the console. No Sequence Options or Sequence Previews are available for this mode. Figure 3.9: Channel(s) configuration window, Digital I/O square Chapter 3. Doric Neuroscience Studio...
  • Page 21 4. The Total Duration (Fig. 3.10) shows the total expected duration of the pulse sequence. Should the duration be infinite, the box will display . If there is an error in parameter selection, this box will display N/A. Chapter 3. Doric Neuroscience Studio...
  • Page 22 5. The Pulse(s) per sequence (Fig. 3.11) set the number of pulses per sequence. If it is set to 0, the number of pulses will be infinite. 6. The Number of sequence(s)(Fig. 3.11) sets the number of times that the sequence will be repeated. 7. The Delay between sequences (Fig. 3.11) sets the delay between each sequence. Chapter 3. Doric Neuroscience Studio...
  • Page 23 • The Sine mode (Fig. 3.12) creates a sinusoidal pulse sequence with peaks at V+ and V-. This includes all sequence options as the CW and Square mode, save the following. – The Time ON option is changed for the Phase option (Fig. 3.12). This allows the choice of the sine wave phase, in degrees. Chapter 3. Doric Neuroscience Studio...
  • Page 24 5. The Order selector is linked to filter sharpness; a higher order will provide a sharper filter. 6. The Cutoff Frequency boxes are used to define the low/high cutoff values for the filter, depending on the type used. The cutoff frequency must be less than half of the sampling rate. Chapter 3. Doric Neuroscience Studio...
  • Page 25 (a) Filter Tab (b) Response Tab Figure 3.14: Linear Mode Filter Options 7. The Response Tab displays the filter response (attenuation) according to frequency. Chapter 3. Doric Neuroscience Studio...
  • Page 26 – The Total Duration displays the total duration of all series. • The Progression box includes the Progression bar, which indicates the progression of the series (in %), while the Time Elapsed counter indicates the progression of the series. Chapter 3. Doric Neuroscience Studio...
  • Page 27 For a data set of 10 points, saved with a Decimation Factor of 2, the first two points (1,2) will be averaged, the second two points (3,4)... This produces a file of 5 points of data Chapter 3. Doric Neuroscience Studio...
  • Page 28 2. The Clear configuration button (Fig. 3.19) resets the acquisition view and all other parameters set. Any configu- rations already set will be lost. 3. The Save configuration button (Fig. 3.19) allows a console configuration to be saved in the .doric format. This file will only preserve the configuration of each current channel.
  • Page 29 TheAcquisition View box displays all information concerning active channels. Each channel chosen using Add Channel is displayed in this window, occupying a rectangular box. Each Channel box shows the following basic elements, with additional elements available for specific channel types. Chapter 3. Doric Neuroscience Studio...
  • Page 30 2. The Edit button (Fig. 3.24) allows the editing of channel parameters, opening the channel configuration windows. For further information, see section 3.2.1. Figure 3.25: Graphs Window 3. The Graph(s) (Fig. 3.24) button opens the Graph Options window. Chapter 3. Doric Neuroscience Studio...
  • Page 31 7. Analog output channels display an Active state graph (Fig. 3.24). This graph displays whether the channel is out- putting a signal (On, V 0) or not (Off, V=0). 8. Output channels display a Preview graph (Fig. 3.24), showing a preview of the pulse sequence. Chapter 3. Doric Neuroscience Studio...

  • Page 32: Electrophysiology Analysis Module

    • The Clear data button deletes all data currently in the module. This data cannot be recovered, so ensure the data is properly saved before clearing it. • The Current File box displays the files currently loaded into the analysis module. Chapter 3. Doric Neuroscience Studio...
  • Page 33 The cursor will be positioned at the same temporal location on each graph. 3.3.3 Processing The Processing tab (Fig. 3.32) is used to process data sets loaded into the module. Chapter 3. Doric Neuroscience Studio...
  • Page 34 – The Cuttoff frequency defines which frequencies are filtered. Which values are accessible depends on the Filter type. • The Find spikes button allows the automated identification of spikes in the data set. See section 3.3.4 for more details. Chapter 3. Doric Neuroscience Studio...
  • Page 35 (Fig. 3.36b, lower left). (a) Spike Finder Results Window, Full display (b) Spike Finder Results Window, Tab Mode Figure 3.36: Spike Finder Results Window Chapter 3. Doric Neuroscience Studio...
  • Page 36 2. The Waveform box (Fig. 3.39) shows a superimposed view of all different spike waveforms recovered. Each dif- ferent color corresponds to a different unit, as shown on the legend. Figure 3.39: Waveform box 3. The Clusters box shows a scatter plot of all spikes. Each color corresponds to a different unit. Chapter 3. Doric Neuroscience Studio...
  • Page 37 Figure 3.41: Unit box 5. The Timeline box shows the original graph with each spike identified. Each color indicates a given unit, as defined in the legend of the Waveform box. Figure 3.42: Timeline box Chapter 3. Doric Neuroscience Studio...

  • Page 38: Specifications

    Specifications Table 4.1: Fi-Wi Opto-electric Cannula General Specifications SPECIFICATIONS VALUE NOTES Peak optical output 8 mW with blue (465 nm wavelength) LED Input current Min: 0, Max: 150 mA Dimensions 7.5 x 9.7 x 9.7 mm Without fiber and electrodes Mass 0.4 g Electrodes material...
  • Page 39 Table 4.3: Electrophysiology Console General Specifications SPECIFICATIONS VALUE NOTES Power supply 110-240 VAC ; 50-60 Hz Power supply adapter included DC Power supply 12 VDC Dimensions 360 x 140 x 37 mm Including connectors TTL input voltage 0 to +5 V Min Hi Level: 4 V;...

  • Page 40: Support

    5.2 Warranty This product is under warranty for a period of 12 months. Contact Doric Lenses for return instructions. This warranty will not be applicable if the unit is damaged or needs to be repaired as a result of improper use or operation outside the conditions stated in this manual.

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