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Advance acoustic Mupid-2plus Instruction Manual

Submarine-type electrophoresis system
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Summary of Contents for Advance acoustic Mupid-2plus

  • Page 2 Japan. This model, the Mupid-2plus, retains all the performance of the Mupid-2 with improved usability based on the ideas of the researchers using it. The Mupid-2plus can be used under exactly the same test conditions as instructed in previous testing manuals for the Mupid-2.

  • Page 3: Before Use

    Before Use Before using this product, be sure you fully understand its features as described in this user manual and use it properly. • Symbols used in this manual and product • Indicates a general precaution or warning. Ignoring this instruction could cause a serious accident.
  • Page 4 Safety Precautions Do not use this product with wet hands. Always use the special-purpose lid and power supply for the migration tank. We are not responsible for any accidents or damage caused by the use of other devices or equipment. The power supply cannot be disconnected while the migration tank lid is closed.
  • Page 5 Mupid-2plus: Components and Functions 1-1 Electrophoresis System Safety plug Handle The safety switch of the power Use this to open and Migration tank lid supply will not turn ON unless the lid close the lid. is set. Safety switch This is turned ON by the lid.
  • Page 6 Gel tray-L: 107 mm (W) × 60 mm (L) [gel size] at once. Standard set contents "Gel maker set L" is standard for Mupid-2plus. The contents of the Gel maker set L are as follows: Gel maker stand-L: Gel tray-S:...
  • Page 7 Gel Preparation (1) Place the gel maker stand level and set the gel tray into the stand. At maximum, four mini gels and two large gels can be prepared at once. If the gel tray is not placed level, the thickness of the gel will not be uniform and migration may be affected.
  • Page 8 (6) Before the solution gels, remove air bubbles from the bottom surface of the gel tray using a glass rod or other tools. Because bubbles on or in the gel will affect migration, they should be drawn out with a dropper, etc.
  • Page 9 Electrophoresis (1) Place the prepared gel on the gel bed of the migration tank together with the tray. Since the side near the power supply is cathode, wells should be placed on the right for migration of nucleic acids in most cases. Wipe off any excess gel that is adhering around and bottom surface of the gel tray.
  • Page 10 (5) After verifying that the operation switch of the power supply is OFF, close the migration tank lid. Do this as follows: 1) First insert the 2 tabs on the cover into the holes in the migration tank. 2) Then rotate the cover forward to close it. (6) Set the desired voltage using the output selection switch.
  • Page 11 Gel Staining and Band Confirmation (1) Verify that the operation switch is OFF, the output indicator LED is unlit and that the platinum electrode is not forming bubbles. (2) Remove the migration tank lid. It can be removed by grasping the handle on the front of the lid and rotating it open.

  • Page 12: Product Maintenance

    Product Maintenance Do not let buffer stand in the migration tank for a long time or re-use it. This can not only prevent accurate test results, but can also cause damage. Detach the power supply when discarding buffer or cleaning the migration tank. When detaching the power supply, push the top with the thumb as illustrated in the figure at right.

  • Page 13: Troubleshooting

    Troubleshooting Causes and Measures Problem - Make sure that the electric cord is plugged into the outlet. The indicator LED - Make sure that the operation button has been pressed. does not light - If the migration tank lid is not closed properly the safety switch and samples cannot go ON;...

  • Page 14: Product Specifications

    Product Specifications Migration tank 187.5 mm (W) × 139.5 mm (L) × 57.0 mm (H) Outside dimensions: Migration tank 133.0 mm (W) × 120.6 mm (L) × 47.5 mm (H) internal dimensions: Buffer capacity: Approx. 250-280 mL (maximum 300 mL when setting gel) Material: PPHOX Weight:...
  • Page 15 Up to 2000 m (6500 ft) Transient overvoltage: Installation Category II Protection class: Pollution Degree 2 Standard set contents Mupid-2plus migration tank: Mupid-2plus migration tank lid: Mupid-2plus power supply: Gel maker set-L: - Gel maker stand-L: - Gel tray-L: - Gel tray-S: - Comb-L:...