Related Manuals for PerkinElmer FlexDrop iQ
Summary of Contents for PerkinElmer FlexDrop iQ
- Page 1 FlexDrop™ iQ™ Non-Contact Dispenser User Manual © Copyright 2021, PerkinElmer, Inc. P/N CLS155330 Rev. A Publication Date: April 13, 2021...
-
Page 2: Preface
This manual is published by PerkinElmer, Inc., 68 Elm Street, Hopkinton, MA 01748 USA. Copyright 2021, PerkinElmer, Inc. All rights reserved, including but not limited to those rights to reproduce this publication or parts thereof. Reproduction of this publication or parts thereof, or the products it describes, by any means or in any form is expressly prohibited without the written permission of PerkinElmer. - Page 3 If applicable, the error number shown in the software or in the log file. Product Service and Customer Support Plans PerkinElmer offers a full range of services to ensure your success. From our original factory warranty through a comprehensive line of customer support plans, PerkinElmer offers you Field Service Engineers and in-house Specialists who are dedicated to supporting your hardware, software, and application development needs.
- Page 4 Lifting hazard. May result in injury. (ISO 3864) Levage dangereux. Peut entraîner des blessures. Keep Dry. (ISO 7000-0626) Garder au sec. Fuse. (IEC 60417-5016) Fusible. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
- Page 5 CE compliance mark. Marque de conformité CE. WEEE symbol (EN50419:2005). Do not dispose of as unsorted municipal waste. See the PerkinElmer website (www.perkinelmer.com) for more information. Consult Instructions for Use. (ISO 7000-1641) Consulter les Instructions d’emploi. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
-
Page 6: Table Of Contents
Selecting the Source Plate ....................19 Viewing the Protocol Overview ..................21 Starting the Dispense Protocol ..................22 Viewing the Results ......................23 Shutting Down the FlexDrop iQ ..................24 Print Log ..........................25 Comma-Separated Values (CSV) File ................26 Header Section......................... 26 Task Section ........................ -
Page 7: Introduction
Introduction Introduction Overview This FlexDrop iQ User Manual contains information about the safe and proper handling and operation of the FlexDrop iQ Non-contact Dispenser and FlexDrop iQ consumables. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc. -
Page 8: Dispensing Runs
2. Press the On/Off switch on the front of the instrument. Wait for the tablet to start and the instrument to initialize. 3. On the tablet, touch the FlexDrop iQ Studio icon to start the FlexDrip iQ Studio software. You are prompted to Open a Saved Protocol or Create a New Protocol as shown in the figure below. -
Page 9: Selecting The Protocol Settings
Select whether to Save liquids in local liquid library (Always, Ask, • or Never). 2. Click OK to define the Target Layout. NOTE: To change protocol settings, click Menu > Settings > Protocol. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc. -
Page 10: Selecting The Target Layout
3. Select the wells which should receive liquids by clicking on the desired well positions shown in the Target Layout tab. Selected wells are highlighted in blue and are defined as one Wellgroup (see Figure 4). P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc. - Page 11 Figure 4. Target Layout Screen with Blue Highlighted Wellgroup NOTE: To add additional target plates, click the Add button in the top left corner of the Target Layout window. The arrow buttons switch between different target plate layouts. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
-
Page 12: Selecting The Dispensing Options
Dispensing Runs Selecting the Dispensing Options The FlexDrop iQ software offers four different dispensing options, which are indicated as buttons Mixing, Dilution, Array, and Normalization on the left below the wellgroup (see Figure 5). To add a dispense option, click the desired button, and then edit the options as described in each section below. - Page 13 Distribution: Choose distribution type of the dilution series. Linear, • logarithmic, and individual dilution factors can be applied. Add replicates. • Change dilution pattern to horizontal or vertical distribution. • P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
- Page 14 Specific volume: Equalize wells to a user-specified volume. • Specific % of assay volume: Equalize well volume to the specified • percentage of the total assay volume. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
- Page 15 Pitch X/Y: Specifies the distance between spots. • Location: Specifies the position of the array within the well. • Choose the wells to be dispensed and the desired dispensing option (see Figure 9). P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
- Page 16 The selected array pattern will be applied to all chosen wells of target layout. Figure 10. Final Array in Target Layout NOTE: Mixing, Dilution, Normalization and Array will affect all wells of the selected area in the respective wellgroup. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
- Page 17 Remove selected wells by dragging them to the bin icon (see Figure 11) or pressing the “Del” key on the keyboard. Figure 11. Editing Wellgroups on the Target Plate P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
- Page 18 Copy Wellgroup button of the respective wellgroup. Select the liquid you want to use and the location for the new wellgroup (see Figure 12). Figure 12. Copy Wellgroup - Setup Screen P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
-
Page 19: Selecting The Source Plate
All liquids and the minimum required volumes for dispensing used in the protocol are listed on the left side of the screen in the Unassigned liquids section (see Figure 14). P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc. - Page 20 Assigned liquids section. Figure 15. Source Plate Setup - Assigned Liquids After defining the source plate layout click Overview in the top bar to proceed. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
-
Page 21: Viewing The Protocol Overview
Figure 16. Overview – Simulation Mode Screen Click Dispense in the top bar to proceed. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc. -
Page 22: Starting The Dispense Protocol
1. After the dispensing protocol is set up, prepare the source plate with respective liquids. 2. Click Start Dispensing. The FlexDrop iQ software prompts you to insert the source plate into the upper tray and the target plate into the lower tray of the device. -
Page 23: Viewing The Results
All used liquids are displayed on the left side of the screen with the corresponding position on the source plate. The FlexDrop iQ software provides information on the performance of each source well. Selecting any target well displays all liquids and volumes dispensed into the selected well on the right side of the window. -
Page 24: Shutting Down The Flexdrop Iq
1. Remove any plates from the Source and Target drawers. 2. Close the Source and Target plate drawers. 3. In the FlexDrop iQ Studio software, select Menu > Exit to close the FlexDrop iQ Studio software. 4. On the tablet, select the Windows button, then select Power > Shut Down. -
Page 25: Print Log
The print log text file, printlog.txt, summarizes the results of a dispensing run. All PrintLogs are labelled with the date and time of the dispensing run and are saved in C:\Users\<username>\FlexDrop iQ Studio\PrintLogs. Figure 19. PrintLog.txt File with Drop Detection Information for Each... -
Page 26: Comma-Separated Values (Csv) File
Protocols can be created using either the FlexDrop iQ Studio software or the CSV format. Exported CSV files are saved in C:\Users\<username>\FlexDrop iQ Studio\Protocols\ by default. The CSV file used on the FlexDrop iQ device consists of a header and a task section. Figure 20. CSV Format for the FlexDrop iQ Application... -
Page 27: Task Section
Enter exact name of liquid for respective dispensing step NOTE The structure and content of the header section is crucial for proper import of the .csv files into the FlexDrop iQ software. The fourth line of the Header should never be changed! REMARQUE La structure et le contenu de la section d'en-tête sont cruciaux... -
Page 28: Advanced User Settings
Advanced User Settings Advanced User Settings These settings are not necessarily required for operating the FlexDrop iQ software but are useful to adjust the dispensing protocols for individual needs. About Liquids and the Liquid Library All data on different liquids and their dispensing parameters are saved in the Liquid Library. -
Page 29: Liquid Class Library
The Liquid Class Library contains the dispensing parameters for different liquid types like aqueous liquids, DMSO, glycerol, solvents, etc., for the FlexDrop iQ Source Plate. Add new liquid classes either by importing provided Liquid Class files (*.libx) or creating new liquid classes. -
Page 30: Creating A New Liquid Class
Measure: Automated calibration of the parameters. Detailed • description in Automated Calibration for new liquid classes on page Manually: Enter parameters manually. Detailed description on page • P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc. -
Page 31: Automated Calibration For New Liquid Classes
Step 1: Define the position of the source well for liquid class calibration. • Step 2: Select the target plate format and position for waste disposal. • Step 3: Select the liquid volume in the source well. • P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc. - Page 32 To start the measurement, select one row of the table, click Measure selected row, and follow the instructions in the FlexDrop iQ software. After the measurement is finished, the FlexDrop iQ software calculates the liquid class from the generated data.
- Page 33 NOTE As soon as the liquid class is saved, it cannot be edited anymore. REMARQUE Dès que la classe liquide est enregistrée, elle ne peut plus être modifiée. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
-
Page 34: Manual Calibration Of New Liquids
NOTE: Increasing the maximum dosing energy might be required for liquids more viscous than water. However, 300 La is recommended and sufficient for most fluids. We recommend increasing in 50 La increments, if necessary. P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc. -
Page 35: About Plate Format Library
About Plate Format Library The plate format library contains target plate formats, which are used with the FlexDrop iQ. Standard SBS format plates like 96-, 384- and 1536-well plates are available by default. New and individual target formats can be created in the Target Plate Editor window (see Figure 28). -
Page 36: About Advanced Settings
Position Settings and Dispense Head Settings. Figure 29. Device Settings Screen – Accessing the Advanced Settings Dispense Head Settings If the FlexDrop iQ dispenses incorrect volumes, re-calibration of the device might be required. CAUTION Before re-calibrating the FlexDrop iQ, please contact your PerkinElmer... - Page 37 Figure 30. Advanced Settings - Dispense Head Settings with Automated Re-Calibration When using the automated re-calibration, the Dispense Head Calibration window guides you through the calibration (see Figure 31): Figure 31. Dispense Head Calibration – Protocol Screen P/N CLS155330 Rev. A FlexDrop iQ User Manual PerkinElmer, Inc.
- Page 38 The FlexDrop iQ counts the number of droplets dispensed and calculates the deviation to the expected number of droplets. Clicking Update calibration moves the current FlexDrop iQ slider values by the offset of the deviation previously calculated (see Figure 32).
-
Page 39: Reset Liquid Classes
Advanced User Settings Reset Liquid Classes To restore the original liquid classes provided by PerkinElmer if they have been deleted or modified, click Reset Liquid Classes in the General Settings window (Figure 33). Figure 33. Restoring Original Liquid Classes A message box displays the Liquid Classes that will be reset and prompts you to confirm that you want to reset the liquid classes listed. - Page 40 PerkinElmer, Inc. 68 Elm Street Hopkinton, Massachusetts 01748 U.S.A. TEL 203-925-4602 FAX 203-944-4904 http://www.perkinelmer.com...