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OLS CASY TTC Operator's Manual

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CELL COUNTER & ANALYZER |

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Summary of Contents for OLS CASY TTC

  • Page 1 CELL COUNTER & ANALYZER |...

  • Page 2: Table Of Contents

    Table of Contents TABLE OF CONTENTS .................... 2 PROLOGUE ......................5 I. Revision History ........................5 II. Contact Address ......................... 5 III. Declaration of Conformity ....................6 EC-Declaration of Conformity ....................6 IV. Warranty .......................... 7 V. Trademarks ........................7 VI.
  • Page 3 4. STARTUP ......................21 4.1 Choosing the Location ....................21 4.2 Assembly and Cabling ..................... 22 4.2.1 Installing the USB/RS232 Adapter ................. 22 4.3 Software Installation ...................... 23 4.3.1 Software installation ..................... 23 4.3.2 Special installation steps for Control devices with touch screen ........24 5.
  • Page 4 7.4 Points to be Observed When Performing Measurements ..........42 7.4.1 Changing the Samples ....................42 7.4.2 Sample Concentration ....................42 7.4.3 Measuring Pore Blockages .................... 43 7.4.4 Air Bubbles ........................43 7.4.5 Empty Storage Container ....................44 7.4.6 Measuring Time Too Short .................... 44 7.4.7 Interrupting the Measurement ..................

  • Page 5: Prologue

    Every effort has been made to ensure that all the information contained in the CASY Operator’s Guide is correct at the time of printing. However, OLS OMNI Life Science GmbH & Co KG reserves the right to make any changes necessary without notice as part of ongoing product development.

  • Page 6: Declaration Of Conformity

    III. Declaration of Conformity EC-Declaration of Conformity Translation Manufacturer: OMNI Life Science GmbH & Co. KG Karl-Ferdinand Braun Straße 2 28359 Bremen Germany OMNI Life Science GmbH & Co. KG declares that the product Product name: CASY TTT ID No.: 5651736, 5651737, 5651738 to which this relates, meets the protection requirements laid down in: Council Directive 2014/30/EU on the alignment of the legal provisions of the Member States on...

  • Page 7: Warranty

    IV. Warranty The warranty conditions are specified in the sales contract. Contact your OLS representative for further information. Any unauthorized modification of the instrument invalidates guarantee and service contract. V. Trademarks CASY is a trademark of OMNI Life Science GmbH & Co KG. Other brands or product names are trademarks of their respective holders.

  • Page 8: Warnings And Precautions

    VIII. Warnings and Precautions Handling Requirements It is important to observe the following safety instructions and guidelines in order to ensure safe and reliable operation of the CASY: Place the instrument on a solid, dry work table that is exposed to neither strong mechanical vibrations nor high-volume noises (e.g., ultrasonic baths).

  • Page 9: General Precautions

    As a rule, such equipment must be sterile before it is passed on for final disposal. For more information contact your local OLS support personnel. The instrument should also be decontaminated prior to shipping it for outside service or repairs.

  • Page 10: Basic Equipment

    1. BASIC EQUIPMENT The basic equipment of CASY includes the following components: Measuring unit with sample platform, waste container, CASYton storage container and control unit (computer is not automatically included and has to be ordered separately) One to three precision measuring capillaries (depending on version) External electrode Protection shield including cable Adapter and USB cable for communication between control and measuring unit...

  • Page 11: Introduction

    2. INTRODUCTION The technology of the CASY System is based on extensive experience gained in analytical working methods in the fields of cytobiology, microbiology, biochemistry, and physics. Its ergonomic and easy operation were developed specifically for day-to-day, routine use. Standardized cell counting methods and accurate measurement of cell viability, cell aggregation, and cell volume with high measuring range dynamics make the CASY Cell Counter and Analyzer an indispensable tool for the qualitative and quantitative assessment of cell cultures and many other sample types.

  • Page 12: Digital Pulse Processing

    2.2.1 Digital Pulse Processing Pulse Area Analysis was developed in 1987 on the basis of Digital Pulse Processing (DPP), a technique developed for the digital recording of electrical signals more than 40 years ago. For cell analysis, DPP is combined with a high scanning rate and a highly standardized method of analyzing cell signals.

  • Page 13: Pulse Area Analysis

    2.2.2 Pulse Area Analysis The measuring signal (for example, the electrical pulse generated by a cell when passing the measuring pore) is scanned by CASY at high frequency (1 MHz). As well as the amplitude of the measuring signal, it also records the shape and time course of the signal as a whole (see Figure The CASY calculates the integral of the measuring signal from the individual readings.
  • Page 14 By analyzing cell cultures, this means that you can analyze everything from very small debris particles up to very large cell aggregates simultaneously. Figure 3 shows a typical size distribution of an animal cell line generated by the CASY Cell Counter and Analyzer. Based on the precisely determined cell volumes, the CASY Cell Counter and Analyzer calculates the corresponding cell diameters, which are displayed together with their associated absolute counts, resulting in a high-resolution size distribution.

  • Page 15: Viability Determination Using Electrical Current Exclusion

    2.3 Viability Determination Using Electrical Current Exclusion The CASY Systems uses a label-free, non-invasive method, called Electrical Current Exclusion, for measuring cell viability of mammalian cell lines. Viability determination may also be possible with other eukaryotic cells, for example algae. As with well-known dye-based assays, this viability determination depends on the membrane integrity of the cells.

  • Page 16: Aggregation Correction

    2.4 Aggregation Correction The CASY instrument offers a method to analyze even clumpy cell suspensions with its integrated aggregation correction. By analyzing the size distribution, CASY determines the most common cell size and the volume of the statistically “representative single cell” (see Figure 5).

  • Page 17: Device Configuration

    3. DEVICE CONFIGURATION The CASY combines a high degree of ergonomics with functionality. The measuring unit is controlled and the measuring data is evaluated and displayed by a computer (not included in CASY part number). This guarantees simple and easy-to-learn operation and a high degree of operating reliability.
  • Page 18 Control Unit An inspection window, which also enables the vertical tube to be monitored visually, is situated above the sampling chamber. In the inspection window you can see three light-emitting diodes, which indicate the filling level of the vertical tube. The green light-emitting diode indicates the start of a measurement, the lower red light-emitting diode indicates when the 200 µl level is reached, and the upper red light-emitting diode indicates when the 400 µl level is reached.

  • Page 19: Measuring Capillary And External Electrode

    3.2 Measuring Capillary and External Electrode The external electrode (see Figure 6) is made of platinum, arranged in front of the capillary, can rotate freely, and be pulled downwards if necessary. The measuring capillary (see Figure 6) is made of highly resistant plastic. It is secured to the sampling chamber cover by a cap nut.

  • Page 20: Pressure System And Power Supply

    3.4 Control Device The CASY can be controlled by computer, notebook or tablet fulfilling the following requirements below. It can be ordered separately through OLS OMNI Life Science or your local distributor. Operating System: Microsoft Windows 10 Windows Fall Creators Update must be installed (at least Windows Version 1709 Build 16299.192)

  • Page 21: Startup

    4. STARTUP 4.1 Choosing the Location The CASY is a precision measuring instrument. To ensure fault-free measuring operation, consider the points listed in section VIII. Warnings and Precautions. You must choose the location according to the following criteria: Place the instrument on a solid, dry work table that is exposed to neither strong mechanical vibrations nor high-volume noises (e.g., ultrasonic baths).

  • Page 22: Assembly And Cabling

    4.2 Assembly and Cabling Connect your CASY System to an interference-free measuring instrument socket. Under no circumstances you should operate the instrument from the same socket as laboratory equipment that works with high switching loads (autoclaves, centrifuges, agitators, etc.). You must also ensure that all laboratory equipment situated in the same vicinity is interference- free/fault-free.

  • Page 23: Software Installation

    4.3 Software Installation Each CASY is supplied with Activation keys that provide one license of CASY software to be installed on the control unit and one additional analytic version that can be installed on another computer. The software tool to install the software, the Universal Installer, is provided on an USB Stick in the accessory.

  • Page 24: Special Installation Steps For Control Devices With Touch Screen

    4.3.2 Special installation steps for Control devices with touch screen It is recommended to disable Edge support (Administrative rights needed). This will avoid opening the action center when swiping in from the right side of your screen. gpedit.msc) Open the Local Group Policy Editor ( In the left pane of Local Group Policy Editor, navigate to the location below.

  • Page 25: Maintenance And Care

    5. MAINTENANCE AND CARE The CASY System is designed to be low-maintenance; the fluid system, however, requires regular attention to guarantee fault-free work over several years. Remember that you are working with biological material. Residues in the fluid system or growth of bacteria, algae, or yeast cells may lead to blockages in the measuring pore, high background, or other faults during operation.

  • Page 26: Weekly Cleaning Wizard

    5.1 Weekly Cleaning Wizard Weekly Cleaning should be performed regularly once a week for optimal performance of the instrument. The software keeps record of the last performed cleaning and will remind you in case last cleaning is more than one week ago. Weekly Cleaning Wizard is accessible from Service Menu (see Figure 8).
  • Page 27 1. Perform Weekly Cleaning Wizard (see section 5.1) 2. Replace CASYton with distilled water – put two CASYcups with distilled water under the capillary and instead of the CASYton container. Purge 3 times. 3. Repeat Step 2. 4. Replace CASYcups with destilled water by two empty cups. 5.

  • Page 28: Operation Of Casy

    Always switch on the CASY instrument first before starting the CASY Control Software. For Control Devices not purchased through OLS OMNI Life Science, installation of the Control Software is required (see section 4.3) After start of the CASY Control Software the instrument will perform a self-test.

  • Page 29: Dashboard And Software Functions

    6.2 Dashboard and Software Functions After software is started, the dashboard will appear. It provides easy access to all available functions of the software. Figure 11: Dashboard allowing fast measurements or access to all relevant functions Some functions are exclusively related to control of the Measuring Unit. If the connection between Measuring und Control Unit fails or if the Accessory/Desktop Version of the Software is started, those functions are not accessible.

  • Page 30: System Settings

    Mean View - generates a mean curve / result from the selected measurements. Table View - Data of the selected measurements can be analyzed, deleted, customized and exported. Templates (left sidebar) - represent the protocol and setting used for measuring specific samples.

  • Page 31: User Management

    6.2.2 User Management CASY differentiates between three distinct security levels User - has the lowest level of rights. Basically users can use predefined templates to measure samples. Operator - Additional to the rights of users, operators are able to create new templates or to modify measurements like the settings for cursors Supervisor - highest levels of rights.

  • Page 32: Service

    6.3 Service The Service menu (see section 6.2) includes commands required to service the system. Some of the commands are accessible all time, while access to some functions is only possible after entering a valid Service Pin. These additional functions are not part of this manual. Also see section 5.

  • Page 33: Start Self-Test

    6.3.2 Start Self-Test This command starts the Self-Test for the measuring system, which is also executed during the startup process. The following checks are carried out: Check of the 12V and 24V voltage supply to the measuring electronics. Check of the real-time clock in the measuring electronics. Check of program and working memory in measuring electronics.

  • Page 34: Performing And Evaluating Measurements

    7. PERFORMING AND EVALUATING MEASUREMENTS Performing measurements requires settings that need to be entered before. In regular use, these settings will be stored in sample specific Templates and measurements can be started by two clicks. If no suitable Template is available, it needs to be generated as described in this section.

  • Page 35: Preparing Samples

    150 µm measuring capillary: < 100 Counts/ml 60 µm measuring capillary: < 200 Counts/ml 45 µm measuring capillary: < 3000 Counts/ml If the system fails to become clean even after several REPEATS, you should carry out the Weekly Cleaning Wizard as described in section 5.1. You must ensure that all vessels used to prepare the samples, the CASYton used to flush the system and dilute the samples, and also any CASYcups used, are virtually free of grease and particle impurities.

  • Page 36: Measuring Samples And Displaying The Size Distribution

    7.3 Measuring Samples and Displaying the Size Distribution Rotate the recess in the sampling platform until it points forward and remove the CASY cup from below the measuring capillary. Place the sample to be measured below the measuring capillary and ensure that the external platinum electrode is immersed in the sample.

  • Page 37: Options For Measurement Results

    Figure 17: Result of PBMC- Measurement in Graph View. Four independent Cursor have been set to differentiate between cell populations. Details are displayed below the graph. The curve has been smoothened. 7.3.2 Options for Measurement results Once a measurement is finished, there are several options to continue working with the result: Save the graph Print –...

  • Page 38: Templates

    7.3.3 Templates Templates represent the measurement method. This means they contain all parameters a CASY uses to analyze a specific sample. Consequently they are sample specific. Templates can be created in different ways. Templates can be imported or exported (see Figure 18) Templates can be created: ...
  • Page 39 Figure 19: Manual measurement allows to measure samples without existing Template When the result is displayed it may be necessary to adjust some of the parameters above and repeat the measurement to receive optimal results. When the results meets the above mentioned requirements continue with 7.3.4 Cursor Settings.

  • Page 40: Cursor Settings

    7.3.4 Cursor Settings There are two modes for setting cursors – “Viability” and “Free Ranges”. For each cell type the cursors need to be adjusted individually in the template.  Free ranges can be used to analyze various kinds of samples including heterogeneous samples, e.g.

  • Page 41: Aggregation Correction

    7.3.5 Aggregation correction The CASY software offers 3 Modes to handle aggregation of cells. For details about the principle please refer to section 2.4 Aggregation Correction. The settings for aggregation correction can be adjusted for single measurements in Graph View or for several measurements at the same time in Overlay View. The settings are accessible under Measurement Settings (see Figure 21).

  • Page 42: Points To Be Observed When Performing Measurements

    7.4 Points to be Observed When Performing Measurements For measuring operation, use only CASYton, CASYcups, and CASYclean as specified by OMNI Life Science GmbH & Co KG. The use of alternative measuring solutions, cups, and cleaning agents may lead to incorrect measuring results and cause damage to the fluid system of the CASY Instrument.

  • Page 43: Measuring Pore Blockages

    The coincidence limits do not depend on the positions of your evaluation cursor settings. They are always determined over the entire physical measuring range of the used measuring capillary (approximately 2% to 80% of the measuring-pore diameter). In the case of cell samples, this includes cell debris as well as cell aggregates with a diameter above the scaling range selected for the size axis (X-axis).

  • Page 44: Empty Storage Container

    If this message still appears when the correct template/capillary is selected and cleaning did not solve the problem, contact OLS for technical support. 7.4.7 Interrupting the Measurement You can interrupt a measurement at any time. Click Cancel in the measurement status display.

  • Page 45: Measurement Results

    7.5 Measurement results 7.5.1 Opening Measurement results Individual measurement results can be viewed in Graph view. They will automatically be displayed after a measurement is finished. Stored results can be opened by clicking on measurement results. Stored results can be segmented grouping them in specific “Experiments” and “Groups”. It is recommended using these classifications to organize the results.

  • Page 46: Importing Measurement Results

    Data that can be imported includes measurements in .csy Format (actual software) as well as measurements from CASY TTC and TT. 7.5.5 Exporting Measurement results Data can be exported as CASY date or as Raw data To export data click the arrow key beside “SELECTION”...

  • Page 47: Troubleshooting

    Points to be considered when performing measurements were already discussed in previous sections 7.4. Additional information can be found on the website under www.cellcounting.de. If you cannot solve the problem using the answers given in this section, contact your OLS representative for troubleshooting assistance.

  • Page 48: Appendix

    9. Appendix 9.1 The Liquid/Pressure System The CASY fluid/pressure system consists of a high-quality, maintenance-free vacuum pump (1), the pressure vessel (4) to buffer high and low pressure, the pressure sensor (8), and a number of special long-life valves. The vertical tube with a total of 4 light barriers for the monitoring and control of fluid movements is an integral part of the fluid system.

  • Page 49: Technical Data On Casy

    9.2 Technical Data on CASY Function: Electronic Cell Counter & Analyzer System for determining cell count and cell size distributions, aggregation, and viability. Operating Environment: Indoor use only. Operational Altitude: Up to 2000 m above sea level. Dimensions: Height: 39 cm Width: 31 cm Depth: 39 cm Weight:...
  • Page 50 Analysis/Results High resolution size analysis with 1024 displayed size channels. Precise analysis of the concentration and volume of cells and particles using up to five adjustable cursor pairs. The size distribution can be shown as count or volume distribution. Cell count: Absolute, relative -%- Cell viability: Absolute, relative -%-...
  • Page 51 For life science research only. Not for use in diagnostic procedures. OMNI Life Science GmbH & Co. KG Karl-Ferdinand-Braun-Straße 2 28359 Bremen T +49 421 276 16 9-0 F +49 421 276 16 9-19 info@ols-bio.de www.ols-bio.de www.cellcounting.de...

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