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4. Close the lid and set the speed control on the rotary tool to the desired speed.
Below is a table of the approximate Super FastPrep-2 speed (+/- 1,000
cycles per minute) for each corresponding position of the speed control slide
on the Dremel® 8220 rotary tool, running with an empty tube.
5. While holding the lid closed with one hand, flip the on/off switch on the rotary
tool with the other hand to run the Super FastPrep-2 for the desired period
of time.
Caution: Do not operate the Super FastPrep-2 while the lid is open.
6. After turning off the rotary tool, open the lid and remove the 2mL tube.
Continue with downstream purification/isolation procedure.
Refer to corresponding purification kit instructions for details concerning sub
sequent isolation of DNA/RNA/Protein from lysate material. For a complete listing
of FastDNA, FastRNA, and FastProtein purification and isolation kits please visit
www.mpbio.com/sampleprep.
Cleaning the Super FastPrep-2
Make sure to disconnect the rotary tool from the Super FastPrep-2 before
cleaning. Wear protective gloves (latex/nitrile) during all cleaning procedures.
Clean the Super FastPrep-2 as necessary. The exterior of the Super FastPrep-2 can be
cleaned with a damp (water, dilute ethanol) paper towel or similar. Clean the lysis cham-
ber (exposed when lid is open) by wiping down the inner walls and tube holder. Allow all
components to air dry prior to use.
Should a lysis tube fracture or break in the sample chamber during
processing, stop processing and immediately clean it as recommended above.
Slide
Cycles Per Minute
5
600
10
1,400
15
1,900
20
2,300
25
4,000
30
4,800
9

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