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Summary of Contents for GenScript eBlot
- Page 1 For fast semi-dry electroblotting of proteins from mini polyacrylamide gels to nitrocellulose or PVDF membranes Version No. 08152014 GenScript USA Inc. 860 Centennial Ave. Piscataway, NJ 08854 Tel: 732-885-9188, 732-885-9688 Fax: 732-210-0262, 732-885-5878 Email: product@genscript.com...
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Page 2: Table Of Contents
Protein Transfer System www.genscript.com Table of Contents Product Contents Product Specifications eBlot Protein Transfer Device Accessory Products Introduction Quick Reference Guide Protocols Troubleshooting Examples of Results Technical Support Warranty Appendix... -
Page 3: Product Contents
Protein Transfer System www.genscript.com Product Contents Product Contents, continued Type of products This manual is supplied with the eBlot Protein Transfer Device (L03010). Components for eBlot Protein Transfer Pad are as follows: Component Quantity 1× eBlot Cathode Pad... -
Page 4: Product Specifications
The specifications for eBlot Graphite Electrode are listed below: eBlot Protein Transfer The eBlot Protein Transfer Pads are used with the eBlot Protein Transfer Device. Pads Specifications eBlot Graphite Electrode The specifications of the eBlot Protein Transfer Pads are listed below: Dimensions: 105 mm (l) ×... -
Page 5: Protein Transfer Device
The lower four digits specify the running time of protein transfer in minute and second. The two Status Lights show the working mode of the eBlot Protein Transfer Device. When the right status light is on, the device is switched on and working at transferring mode;... -
Page 6: Accessory Products
Conventional semi-dry blotting is a cumbersome process, requiring time Support or visit www.genscript.com. -consuming reagent preparation and setup, followed by an electrophoretic transfer that could take one hour or more. GenScript’s eBlot Protein Transfer System accelerates the semi-dry blotting process without sacrificing performance. ONE-HOUR Western... -
Page 7: Introduction
· Consumable transfer pads offering convenience without the need for Anode Pad act as ion reservoirs that contain the appropriate anode and additional buffers. cathode buffers. The design of the eBlot Protein Transfer Device reduces · Compatible for use with various types of mini polyacrylamide gels. -
Page 8: Quick Reference Guide
Spong Cushion; for 1.5mm gel, use one or zero piece of Sponge Cushion. Note: After 20 times of protein transfer, replace the used Sponge Cushions with new ones. A pair of new Sponge Cushions are included in each box of eBlot Protein Transfer Pads. -
Page 9: Protocols
Stack, continued Absorbent Filter Paper inside the tray and then push the tray in. eBlot™ Protein Transfer Pads box and tear the laminated sealing of the Note: Change absorbent paper with a new one when opening a new box of pads. - Page 10 Protocols, continued Protocols, continued Assembling the Transfer 6. Place the equilibrated membrane on the eBlot Anode Pad. Gently remove Assembling the Transfer 9. Select appropriate Gel Window according to actual size of the gel (see Stack, continued Stack, continued air bubbles between the membrane and the anode pad using the small the table below).
- Page 11 Protocols, continued Assembling the Transfer Performing Blotting, 11. Press the Open button, and then push back and close the lid of eBlot 3. At the end of the transfer, current automatically shuts off and the eBlot Stack, continued continued Protein Transfer Device.
- Page 12 Min. button for 2 seconds to toggle to numbering mode. If the upper three digits show “100” or a number greater than “100”, perform the replacing protocol as describe below. After successfully installing Graphite Electrode into the eBlot Device, you are ready to use the eBlot Device for another blotting application.
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Page 13: Troubleshooting
Lights flash simultaneously. through the Device. ensure Gel Window covered An Express™ PAGE Gels 8-16% (GenScript MG816W12) was blotted using correctly on the gel. the eBlot . 10.0, 5.0, 2.5, 1.25 0.62, 0.31 and 0.16 μg of HeLa cell lysate were loaded respectively. -
Page 14: Technical Support
If any defects occur in the product during this warranty period, 2. Complete technical support contact information GenScript will, at its option, repair, replace, or refund the purchase price of 3. Access to the GenScript Online Catalog this product at no charge to you. The following defects, however, are 4. -
Page 15: Appendix
Protein Transfer System www.genscript.com eBlot Protein Transfer System www.genscript.com Appendix Appendix, continued - 25 - - 26 -... - Page 16 Protein Transfer System www.genscript.com Appendix, continued - 27 -...
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