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Burleigh PCS-6000 Series Operating Manual

Motorized patch-clamp micromanipulator
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Summary of Contents for Burleigh PCS-6000 Series

  • Page 1 (217) 352-9330 | Click HERE Find the Thorlabs / Exfo / Burleigh PCS-6000 at our website:...
  • Page 2 OPERATING MANUAL PCS-6000 SERIES Motorized Patch-Clamp Micromanipulator 035-00266 (Rev. 0) Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 3 © 2007 EXFO Photonic Solutions Inc. ® Words that EXFO considers trademarks have been identified as such. EXFO ® ® Burleigh and Gibraltar are registered trademarks. However, neither the presence nor absence of such identification affects the legal status of any trademark.

  • Page 4: Table Of Contents

    CONTENTS Introduction……………………………………………………………………………………………..1-1 Getting Started ..........................1-1 Overview ............................1-1 Major Components .........................1-2 Micromanipulator Assembly ....................1-2 Axis Control Unit........................1-2 Joystick .............................1-3 Controller Unit.........................1-3 Glossary of Symbols ……………………………………..............1-3 Safety Warnings ..........................1-4 2. Description……………………………………………………………………………………………….2-1 Mechanisms of Motion ........................2-1 Coarse Motion .........................2-1 Fine Motion ..........................2-1 Configurations ............................2-2 Axes of Motion ...........................2-2 Right and Left Configurations ....................2-3 Manipulator Configurations ....................2-3...
  • Page 5 Single Stepping ........................5-4 Drift Problems......................... 5-4 6. Maintenance ..........................6-1 Overview of Maintenance Required.................... 6-1 Maintenance Schedule ......................6-1 Lubricating Motor Shafts ...................... 6-1 Electronic Maintenance ......................6-6 Replacing the Fuse(s) ......................6-6 Adjusting the Friction of the ACU Knobs................... 6-7 Adjusting the Friction and Eliminating Lateral Play in the Manual Adjustment Knobs ...

  • Page 6: Introduction

    Burleigh PCS-6000 Series Operating Manual 1 Introduction 1. INTRODUCTION Getting Started Thank you for purchasing a PCS-6000 Series motorized manipulator. This manual is intended to provide in-depth information on the operation and use of the PCS-6000. Overview Burleigh PCS-6000 Series patch-clamp micromanipulators from EXFO Life &...

  • Page 7: Major Components

    Burleigh PCS-6000 Series Operating Manual 1 Introduction Figure 1-1: PCS-6000 Series patch-clamp micromanipulator Axis Control Unit (ACU) Major Components Joystick Micromanipulator Assembly The micromanipulator assembly consists of up to four linear stages and two rotary pivot stages assembled with a 90° bracket. Depending on the configuration, the linear stages can have no piezo, a 150 µm piezo or a 300...

  • Page 8: Joystick

    Burleigh PCS-6000 Series Operating Manual 1 Introduction Joystick The joystick controls the speed and direction of the servo motors. The joystick assembly also includes switches to activate specific functions. Controller Unit The control unit contains the electronics that drive the piezo and the servo motors.

  • Page 9: Safety Warnings

    Burleigh PCS-6000 Series Operating Manual 1 Introduction Safety Warnings WARNING Should this equipment be used in a manner not specified by EXFO, the protection provided by the equipment may be impaired. Always follow the operating instructions as indicated! WARNING Refer servicing to qualified personnel. To prevent electrical shock, do not open or remove the cover of the controller or joystick.

  • Page 10: Description

    Burleigh PCS-6000 Series Operating Manual 2 Description 2. DESCRIPTION Mechanisms of Motion PCS-6000 linear stages integrate piezo-flexure assemblies inside crossed roller bearing linear stages with a stepping motor for coarse motion. The joystick is used to control the coarse motion. A large knob on the axis control unit (ACU) controls the fine positioning obtained by use of a piezo.

  • Page 11: Configurations

    Burleigh PCS-6000 Series Operating Manual 2 Description FINE + COARSE STAGE TOP (ATTACHED TO MOTOR) = TOTAL MOTION TOTAL MOTION = SUM OF COARSE AND FINE MOTIONS PIEZO GENERATES FIXED FINE MOTION BY MOTOR MOTOR GENERATES COARSE STAGE EXPANDING/CONTRACTING SHAFT...

  • Page 12: Right And Left Configurations

    Burleigh PCS-6000 Series Operating Manual 2 Description Approach Figure 2-2: Graphic representation of axes Right and Left Configurations A right-hand configuration is used when the manipulator is placed on the right of the microscope. The manipulator is assembled such that the approach axis projects to the left and the rotary stage locking knobs are conveniently located on the outboard (right) side of the manipulator.
  • Page 13 Burleigh PCS-6000 Series Operating Manual 2 Description PCS-6100 PCS-6100 LEFT HANDED RIGHT HANDED VERSION VERSION Figure 2-3: PCS-6100 PCS-6200 This configuration has three axes: Axis 1 – front to back Axis 3 – vertical Axis 4 – approach All three axes have the motorized linear stages with 150-µm piezo elements.
  • Page 14 Burleigh PCS-6000 Series Operating Manual 2 Description PCS-6300 This configuration has three axes. Axis 1 – front to back Axis 3 – vertical Axis 4 – approach All three axes have motorized linear stages. Axis 1 and Axis 3 have 150-µm piezo elements while Axis 4 has 300-µm piezo element.
  • Page 15 Burleigh PCS-6000 Series Operating Manual 2 Description PCS-6400 PCS-6400 LEFT HANDED RIGHT HANDED VERSION VERSION Figure 2-6: PCS-6400 PCS-6500 This configuration has four axes: Axis 1 – front to back Axis 2 – left to right Axis 3 – vertical Axis 4 –...
  • Page 16 Burleigh PCS-6000 Series Operating Manual 2 Description Additional configurations Non-standard configurations are available by special order from the factory. Their product designation is PCS6000 – (XXXX). Special order configurations permit the combination of options to suit the exact experiment. Coarse and fine positioning options are listed in the table below.
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  • Page 18: Orientation

    Burleigh PCS-6000 Series Operating Manual 3 Orientation 3. ORIENTATION Control Unit The control unit supplies the drive signals to both the piezo actuators and the drive motors. The drive board for the motors is on top layer. The drive board for the piezo actuators is on the bottom.
  • Page 19 Burleigh PCS-6000 Series Operating Manual 3 Orientation seconds). Stall is detected by noticing that the Move light is on, but no motion is detected. The motor generates a clicking sound while in stall. Several configurations of the PCS-6000 have less than four motors. In these instances, there are unused motor receptacles.

  • Page 20: Rear Panel

    Burleigh PCS-6000 Series Operating Manual 3 Orientation Rear Panel Joystick Controller: Motors Axes Optional USB Port Controller: (GUI) Motor-Axis 4 Controller: (Piezo) Fuse holder Chassis Ground Power cord Post receptacle Figure 3-2: Rear panel of control unit Operating Voltage The unit contains a universal power supply. No changes are needed to switch from 110V AC 60 Hz to another allowed voltage and frequency (see Section 7, Specifications for details).

  • Page 21: Axis Control Unit (Acu)

    Burleigh PCS-6000 Series Operating Manual 3 Orientation Axis Control Unit (ACU) The axis control unit (ACU) allows you to adjust the piezo elements for fine positioning. The ACU connects to the rear of the PCS-6000 control unit via a nine-pin connector. Depending on the model, it can have as many as four control knobs.

  • Page 22: Joystick

    Burleigh PCS-6000 Series Operating Manual 3 Orientation Joystick Memory Axis 2 (Y): Preset Left to right Positions: 1 & 2 Speed Axis 1 (X): Control Front to Selector back Axis 3 (Z): Vertical or Approach Figure 3-4: Joystick- Servo Motor Control The joystick is used to control the servo motors to provide coarse positioning.
  • Page 23 Burleigh PCS-6000 Series Operating Manual 3 Orientation Moving the joystick results in “velocity control.” This means that the farther from the rest position the joystick moves, the faster the motor runs. This is similar to the gas pedal of an automobile − the farther it is pushed, the faster the car runs.
  • Page 24 Burleigh PCS-6000 Series Operating Manual 3 Orientation PCS 6000 Graphical User Interface When the PCS-6000 is connected to a PC via a USB cable, the controller status is immediately available via the PCS-6000 control panel. The following screen shot shows the control panel and the available controls.

  • Page 25: Graphical User Interface

    Burleigh PCS-6000 Series Operating Manual 3 Orientation Graphical User Interface for PCS-6000 control panel The graphical user interface provides 2 modes of operation. When the GUI “Connect” is enabled it provides remote PC control of the 3 servo motor axes provided with the manipulator. This control is provided by the jog and joystick controls available on the interface.
  • Page 26 Burleigh PCS-6000 Series Operating Manual 3 Orientation Controlling unit functions via the graphical user interface: Please refer to the screen shot of the graphical user interface on the page 3-6 for the available functions as detailed below: Connect / Disconnect –...
  • Page 27 Burleigh PCS-6000 Series Operating Manual 3 Orientation Set Mem 2 - This button sets the current position displayed as the memory position 2. When the memory position has been set, the step or um position will be displayed in the Memory Position 2 box, and the green status bar will be illuminated.
  • Page 28 Burleigh PCS-6000 Series Operating Manual 3 Orientation Jog Speed - The Jog speed control changes the speed at which the jog controls will move the manipulator. Note: The controller will not operate with the speed set at < 15% Joystick Control - The Joystick control moves the manipulator, the speed increases as the joystick is moved from the centre of the control.

  • Page 29: Serial Port Communication

    4. Click on the “Device Manager” button. 5. Expand the “Ports (COM & LPT)” item in the tree. 6. Find the “Exfo Burleigh PCS-6000 Controller (COMXX)”. The XX is the virtual COM port number on your system. 7. Close the Device Manager, the System dialog box and the control panel.
  • Page 30 Burleigh PCS-6000 Series Operating Manual 3 Orientation The serial port commands to control the PCS-6000 controller are as follows: The communications structure is split into two sections, the first section is the commands that are transmitted to the PCS-6000 controller, and the second section is the responses that are received by the PC.
  • Page 31 Burleigh PCS-6000 Series Operating Manual 3 Orientation ‘Q’ Transmission Packet The ‘Q’ Packet has 3 bytes which are outlined in the following table. Byte Name Description Packet Header The packet header in a ‘Q’ Packet is the character ‘Q’. Carriage Return The second last byte of a ‘Q’...
  • Page 32 Burleigh PCS-6000 Series Operating Manual 3 Orientation ‘D’ Transmission Packet The ‘D’ Packet has 3 bytes which are outlined in the following table. Byte Name Description Packet Header The packet header in a ‘D’ Packet is the character ‘D’. Carriage Return The second last byte of a ‘D’...
  • Page 33 Burleigh PCS-6000 Series Operating Manual 3 Orientation ‘O’ Response Packet The ‘O’ response packet is received when a command is sent to the PCS 6000 controller correctly. The ‘O’ response is returned for all command except ‘Q’ and ‘M’ which have their own response packets described below.
  • Page 34 Burleigh PCS-6000 Series Operating Manual 3 Orientation Z Position These 3 bytes contain the 24-bit variable representing the current position of Motor 3. Status This byte is such that each of its bits is as follows: bit 7: has a value of 1 if the unit is in the ‘C’...
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  • Page 36: Installation

    Burleigh PCS-6000 Series Operating Manual 4 Installation 4. INSTALLATION Unpacking the Unit Carefully unpack the unit and inspect for shipping damage. Severe damage to the packaging may indicate component damage. Notify your shipping/receiving department immediately. Removing the Shipping Clips To prevent damage to the stages and piezo during shipment, the stage top and bottom are displaced from their normal positions with shipping clips.

  • Page 37: Mounting The Manipulator

    Burleigh PCS-6000 Series Operating Manual 4 Installation Mounting the Manipulator 1. Locate the rotation plate adapter and determine the approximate location where it is to be mounted. 2. If the mounting surface has a grid of ¼ -20 tapped holes, use the hardware supplied to mount it to the mounting surface.

  • Page 38: Mounting A Headstage And Pipette

    Burleigh PCS-6000 Series Operating Manual 4 Installation 6. Position the joystick and ACU in a convenient location in the workstation. Plug the cables into the back of the control unit. The joystick and ACU are set up so that the cables from the units exit away from the operator.
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  • Page 40: Operation

    Burleigh PCS-6000 Series Operating Manual 5 Operation 5. OPERATION Powering Up and Checking for Motor Motion 1. Plug in the AC line cord and turn on the control unit. After a second or two, the power indicators light on both the upper (motor) and lower (piezo) modules.

  • Page 41: Adjusting The Approach Axis Angle

    Burleigh PCS-6000 Series Operating Manual 5 Operation Adjusting the Approach Axis Angle The Approach Axis can be set to any angle. Figure 5-1: Adjusting the angle of the Approach Axis 1. Use the joystick to adjust each of the motorized stages to the center of travel.

  • Page 42: Setting The Adjustable Stop

    Burleigh PCS-6000 Series Operating Manual 5 Operation Setting the Adjustable Stop The adjustable stop mechanism on each rotary pivot stage consists of a locking ring, two set screws, a pin and a brass block. Figure 5-2: Adjustable stop mechanism 1. Position the headstage at the desired angle (see previous procedure).

  • Page 43: Piezo Control

    Drift Problems Stability PCS-6000 series micromanipulators have the ability to hold the position of its stages to high precision. The performance specification is less than 1 µm per hour per axis. When the observed stability is not at this level, many times there are a number of causes.

  • Page 44: Maintenance

    Burleigh PCS-6000 Series Operating Manual 6 Maintenance 6. MAINTENANCE Overview of Maintenance Required Maintenance Schedule PCS-6000 systems require a limited amount of routine maintenance. Items requiring periodic attention are listed below: Interval between Item Comments service First month and every...
  • Page 45 Burleigh PCS-6000 Series Operating Manual 6 Maintenance 10 mm Fig 6-1: Align stages before turning off the power 2. Remove manipulator from the Faraday cage and place on a well-lit work table. 3. Holding the stage as shown in Figure 6-2, shift the top to take pressure off of the motor.
  • Page 46 Burleigh PCS-6000 Series Operating Manual 6 Maintenance Fig 6-3: Remove the screw by turning counter-clockwise 7. Apply lubricant to the screw (Figure 6-4). Only a small bead of lubricant is needed along the central 1 inch of shaft. Fig 6-4: Apply lubricant along length central 1” of the motor shaft 8.
  • Page 47 Burleigh PCS-6000 Series Operating Manual 6 Maintenance Anti-rotate pin is perpendicular to the motor hatch cover Fig 6-6: Anti rotate unit fully retracted and angled for assembly 11. Locate the anti-rotate slot in the moveable stage (Figure 6-6). Anti-rotate slot Fig 6-7: Location of the anti-rotate slot 12.
  • Page 48 Burleigh PCS-6000 Series Operating Manual 6 Maintenance Figure 6-8: Insert into slot and loosely tighten the 4-40 screws 14. Displace the move able section of the stage to expose the end of the shaft. Verify that pin is in slot (see Figures 6-9 and 6-10).

  • Page 49: Electronic Maintenance

    Burleigh PCS-6000 Series Operating Manual 6 Maintenance Figure 6-10: Incorrect alignment (pin is not captured in the slot) 15. If the alignment is correct, finish tightening the 4-40 screws that hold the motor module to the stage. Electronic Maintenance There are no maintenance or user-serviceable items inside the control unit, ACU or joystick.

  • Page 50: Adjusting The Friction Of The Acu Knobs

    Burleigh PCS-6000 Series Operating Manual 6 Maintenance Lift out the fuse holder Figure 6-11: Removing the fuse holder Location of fuse(s) Figure 6-12: Removing and replacing the fuse Be sure to replace fuse(s) with the same rating: T-type 4.0A, 250V Adjusting the Friction of the ACU Knobs To adjust the friction of the control knobs on the ACU.

  • Page 51: Adjusting The Friction And Eliminating Lateral Play In The Manual Adjustment Knobs

    Burleigh PCS-6000 Series Operating Manual 6 Maintenance Lock screws Figure 6-13: Adjusting the friction of an ACU knob Adjusting the Friction and Eliminating Lateral Play in the Manual Adjustment Knobs The sleeve can be rotated to adjust the friction and lateral stability of the adjustment knobs on the manual stages.

  • Page 52: Troubleshooting

    Burleigh PCS-6000 Series Operating Manual 6 Maintenance CATCH HOLE Figure 6-15: Locating the catch hole 2. Turn the wrench clockwise to tighten the sleeve, increasing the friction (Figure 6-16). Repositioning the wrench underneath and turning counter- clockwise loosens the sleeve, decreasing the friction of the adjustment knob.

  • Page 53: Problem: No Fine Motion

    Burleigh PCS-6000 Series Operating Manual 6 Maintenance Also refer to Drift later in this section. Probable Causes Instability generally is caused by loose mechanical connections or dirt/debris between the mating surfaces. What to Check Micromanipulator and Linear Stages Check that all screws are tight.

  • Page 54: Problem: No Coarse Motion

    Burleigh PCS-6000 Series Operating Manual 6 Maintenance The cable to the ACU may have become disconnected at the back of the control unit. What to Check Cables for linear stages are properly plugged in. Visually check for interference. Check that no part of the micromanipulator is rubbing or hitting the microscope mount.

  • Page 55: Problem: Drift

    Burleigh PCS-6000 Series Operating Manual 6 Maintenance Problem: Drift Typical Symptoms Pipette tip moving after manual and piezo adjustments are completed. "Losing" cells after making a seal. Also refer to Section 6, Troubleshooting, for information on excess motion of the pipette tip during manual adjustment.

  • Page 56: Specifications

    Burleigh PCS-6000 Series Operating Manual 7 Specificatio 7. SPECIFICATIONS Performance: Travel Piezo Motorized Manual 150 or 300 µm 25 mm nominal 25 mm nominal Resolution 60 nm (150 µm) OR 1.6 µm minimum 32 threads per inch lead 120 nm (300 µm)

  • Page 57: Product Safety

    Burleigh PCS-6000 Series Operating Manual 7 Specifications Product Safety: IEC 61010-1:2001 Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use Part I. EN 61010-1:2001 Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use CAN/CSA C22.2 No. 61010-1- Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use Part I;...
  • Page 58 Burleigh PCS-6000 Series Operating Manual 7 Specificatio WEEE Directive (2002/96/EC) The symbol above indicates that this product should not be disposed of along with municipal waste, that the product should be collected separately, and that a separate collection system exists for all products that contain this symbol within member states of the European Union.
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  • Page 60: Warranty

    WARRANTY Burleigh PCS-6000 Series motorized patch-clamp micromanipulators are warranted against defects in material and workmanship for a period of two years after date of delivery. During the warranty period, EXFO Life Sciences & Industrial Division will repair or, at its option, replace parts that prove defective when the instrument is returned prepaid to EXFO Life Sciences &...
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  • Page 62: Index

    Index ACU..........3-4, 6-8 Electronics ..........6-6 Adjustments Lubricating motor shafts......6-1 ACU control knob friction......6-8 Replacing the fuse ........6-6 Approach Axis angle ........5-2 Maintenance schedule ......6-1 Major components .........1-2 Friction of ACU knobs ........6-8 Mechanism of motion Friction of manual adjustment knobs ...6-8 Coarse ............
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  • Page 64 Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...
  • Page 65 Toll free: 1.800.668.8752 (USA & Canada) www.exfo-lifesciences.com burleigh@exfo.com EXFO Electro-Optical Engineering Inc 400 Godin Ave Quebec City, Quebec G1M 2K2 Canada Tel: 1.418.683.0211 | Fax: 1.418.683.2170 PCS-6000 Series Motorized Patch-Clamp Micromanipulator Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com...

This manual is also suitable for:

Pcs-6100Pcs-6200Pcs-6300Pcs-6400Pcs-6500

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