Tokai Hit STX-E Series Instruction Manual

Stage top incubator

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MA-STX-E-EN-01

Stage Top Incubator

STX –E series

Instruction Manual

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Summary of Contents for Tokai Hit STX-E Series

Page 1 MA-STX-E-EN-01 Stage Top Incubator STX –E series Instruction Manual...
Page 2 Index For safe and comfortable use ・・・・・・・ 1 System Structure ・・・・・・・・・・・・・ 1.1 Components 2 Controller ・・・・・・・・・・・・・・・・ 2.1 Size and weight 2.2 Name of parts 2.3 Specifications 3 Chamber ・・・・・・・・・・・・・・・・・ 3.1 Structure 3.2 Installation 3.3 How to set vessel to Dish Attachment 3.4 How to set Dish Attachment to chamber 3.5 How to set Lens Heater 3.6 How to set Temperature Sensor...
Page 3 For safe and comfortable use About symbols and indications These warnings enable safe and correct usage, and prevent dangerous situations. Please follow them at all times. Symbol meaning WARNING Indicates a hazardous which could result serious injury. CAUTION Indicates a situation which could result slight injury. Indicates a situation which could damage the equipment CAUTION or other apparatus.
Page 4 To all users This device is to be used by researchers doing cultivation observations in a laboratory. It cannot be used at home. Do not take the equipment apart. This results in damage. When shipping Please treat this precision instrument with care. Impacting it is forbidden.
Page 5 Connect the cylinder to the controller with supplied tube and fasten with band. Using another tube causes breakage and escape of gas. If the tube is not fastened well, there is a risk that it comes off. Do not bend the silicon tubes. If they are plugged, gas cannot be adequately supplied to the chamber.
Page 6 To pull out the plug from the outlet swiftly in an emergency, do not place any things near the outlet. Storage Turn off power supply while performing maintenance, When opening the chamber, do not inhale air in the chamber directly. It may adversely affect human bodies.
Page 7 1 System Structure 1.1 Components Name of components Controller * STXG or STXF Chamber Lens Heater Dish Attachment Note Fixing Lid Temperature Sensor Extension Wire for Temperature Sensor Gas Tube Precision Screw Driver Spare coverglass for LX-D35 Power Cable １ Disposable Syringe for Draining Water Note：...
Page 8 2 Controller 2.1 Size and weight Model Dimension Weight STXG W151xD263xH196 mm 3.8 kg STXF W151xD296xH196 mm 3.5 kg 2.2 Name of parts The image is shown for reference only. Front panel STXG with built-in gas mixer STXF with analog flowmeter ①...
Page 9 Rear Panel STXG with built-in gas mixer STXF with flowmeter ⑥ ⑥ ⑤ ⑤ ④ ④ ③ ③ ② ② ① ① ⑦ ⑦ Name Role Note ① USB Connection Connection to PC For connection to PC, see P.25. Use only if the system is used ②...
Page 10 2.３ Specifications STXG STXF with built-in gas mixer with analog flowmeter Temperature control PID control Increments 0.1℃ step Setting method Touch Panel or PC (optional, STX-APP requires) SAMPLE TEMP 30℃ - 40℃ (optional, STX-FB requires) Possible temp. TOP HEATER 10℃ - 65℃ setting range BATH HEATER 10℃...
Page 11 3 Chamber 3.1 Structure The image is shown for reference only. WSKMX Chamber ① ⑤ Wellplate ⑥ ② Gas Inlet Sensor/Tube Fixing Block Water Inlet ③ Objective Lens ④ Name Role Note ・Condensation prevention ① Top Heater ・Sample temperature control Presence/absence of Bath ・Humidification inside of ②...
Page 12 3.2 Installation WSKMX / IX3WX Applicable vessels Applicable stage Flat-top XY stages with 160 x 110 mm aperture Installable on various stages with optional adapters. Dimension Recommended water volume W187 × D146 × H27 mm 32cc Installation Fit the bottom part of chamber into the aperture of XY stage. For use on Prior Scientific H117 stage, see the next page and remove 3.0mm spacer from the bottom of chamber.
Page 13 Installation of WSKMX/IW3WX on Prior Scientific H117 Before installing WSKMX/IX3WX on Prior Scientific H117 stage, remove 3.0mm Spacer from the bottom of chamber. ① Remove Top Heater (chamber lid). ② Turn the chamber upside down and remove the 4 screws with Precision Screw Driver that comes with the product.
Page 14 WELSX Applicable vessels Applicable stage XY stage with Tokai Hit Stage Adapter or sample holder which can accommodate wellplate Dimension Recommended water volume W127.5 × D85 × H27 mm 21cc Installation Fit the bottom part of chamber into the aperture of Stage Adapter...
Page 15 TIZWX Applicable vessels Applicable stage Nikon Ti2/Ti piezo stage Optional Stage Adapter requires for Ti2/Ti manual/motorized stage. Dimension Recommended water volume W175.5 × D118 × H30 mm 26cc Installation Remove Top Heater and use two screws to fix the chamber on piezo stage or optional Stage Adapter.
Page 16 TIZBX Applicable vessels Applicable stage Nikon Ti2/Ti piezo stage Optional Stage Adapter requires for Ti2/Ti manual/motorized stage. Dimension Recommended water volume W178 × D118 × H32 mm 61cc Installation Remove Top Heater and use three screws to fix the chamber on piezo stage or optional Stage Adapter.
Page 17 GSI2X Applicable vessels Applicable stage Leica Super Z galvo stage Dimension Recommended water volume W126 × D96 × H23 mm 18cc Installation Remove Top Heater and fix the chamber to stage by four screws on the side of chamber.
Page 18 DMIWX Applicable vessels Applicable stage Leica Scanning stage with condenser cover Recommended water volume Dimension W170 × D110 × H27.5 mm 19cc Installation Fit the bottom part of chamber into the aperture of XY stage.
Page 19 WSBX Applicable vessels Applicable stage Zeiss Piezo Insert WSB 500 Dimension Recommended water volume W127.5 × D85 × H27 mm 18cc Installation Fix the chamber to stage by four hexagon socket screws.
Page 20 3.3 How to set vessel to Dish Attachment See the figure below and set vessel. 35 mm dish 50/60 mm dish LX-D35 LX-D56 Adapter for 35 mm dish ATX-D Use Adapter for 35 mm dish Remove Adapter for 35 mm and fix by magnets of Fixing dish and fix by magnets of Lid LX-D35.
Page 21 3.4 How to set Dish Attachment to Chamber WSKMX/IX3WX/TIZWX/DMIWX/WSBX ATX-D/CSG ＋ ATX-A ATX-W Chamber Attach ATX-D/CSG to ATX-A and install it into the chamber with Attachment for wellplate ATX-W. WELSX/TIZBX/GSI2X ATX-D/CSG Chamber Install ATX-D/CSG directly into the chamber.
Page 22 3.5 How to set Lens Heater Wrap Lens Heater so as to be in close contact with the objective lens. Avoid uneven surface such as correction collar and diaphragm. The cable has magnetic Relay Connector to prevent the cable from getting entangled around the objective revolver.
Page 23 3.6 How to set Temperature Sensor Set Temperature Sensor to Fixing Lid. Temperature Sensor * Temperature Sensor is autoclavable. (121℃, 2 atmospheric pressure, 20 minutes) Fixing Lid Set Temperature Sensor so that the tip is dipped properly into media in the vessel.
Page 24 3.7 Access Ports Set Fixing Blocks properly according to use/non-use of Temperature Sensor and tubes. Port for 2 tubes Installation of two silicone tubes with diameter of 3 mm. (For perfusion and media exchange) Port for 2 tubes Installation of two silicone tubes with diameter Hexagon nut of 3 mm.
Page 25 4 Connection 4.1 System Image Gas Regulator STXG： Use 100% CO STXF： Use 5% CO premixed gas Gas Tube Gas Tube Chamber ＋ Lens Heater Extension Wire PC Operation via Software STX-APP(Option) Temperature Sensor Link Connector A Connected when using the system with separately sold optional gas mixers.
Page 26 4.2 Connection of Gas Tubes Controller STXG Connect Gas Tube between the regulator of 100% CO2 and CO2 IN at the rear panel of controller. STXF Connect Gas Tube between the regulator of 5% CO2 premixed gas and CO2 IN at the rear panel of controller.
Page 27 5 Operation 5.1 Touch Panel HOME STXG STXF < TOP HEATER / BATH HEATER / STAGE HEATER / LENS HEATER > Shows Process Value and Setting Value of Top Heater, Bath Heater, Stage Heater and Lens Heater. When one area is tapped in each heater area, Setting Value can be changed.
Page 28 SETTING Tapping SETTING button, the display changes to the screen for ON/OFF setting of heaters and gas supply, the reference setting value of Lens Heater and parameters. < ON/OFF setting > You can turn ON/OFF for Bath Heater, Lens Heater and gas supply. When using the chamber which does not have Bath Heater, the Bath Heater is set to OFF in advance.
Page 29 < Advance Setting > You can change Input Correction values and Control Parameter values. The values calculated by calibration at Tokai Hit are inputted at the time of factory shipping. Do not change the default values for any purpose other than changing the chamber. The default value are printed on the top surface of controller.
Page 30 < Optional Gas Mixer > When using the system with optional gas mixers, tap GAS button at HOME screen and set CO concentration and gas flow. Select module For STX-CO2O2 For STX-CO2 System Image of optional gas mixers STX-CO2O2 STXG STX-CO2 STX-O2 STXF...
Page 31 5.2 Reference Setting Value Reference Setting Value is described at the seal at front panel of controller. It is provided to heat the dish bottom temperature to 36.5℃- 37.0℃ under room temperature of 25℃. Temperature inside chamber may vary depending on actual use condition. example Shows Reference Setting Value for 35mmDish...
Page 32 5.4 Preliminary Operation In order to stabilize the environment inside/outside the chamber, carry out preliminary operation for about 1 hour before experiment. Making the temperature of microscope and chamber before observation keeps the sample temperature stable and prevents drift of the observation image. Remove top heater (chamber lid) and install the suitable dish attachments and the empty vessel.
Page 33 5.5 Gas Supply 1) Confirm regulator valve ② is closed. 2) Confirm pressure adjustment ③ is slack. 3) Open cylinder valve ①. 4) Open regulator valve ②. 5) Turn pressure adjustment ③ toward right to adjust secondary pressure indicated in meter B to be in a range from 0.1MPa to 0.15MPa.
Page 34 5.6 Cultivation A Reference Setting Value Confirm room temperature is stable at around 25℃. 25℃ Remove the empty vessel from the chamber and install the observation vessel. For the setting of vessel, see P.20. For the setting of attachment, see P.21. Confirm FeedBack switch is OFF.
Page 35 5.7 Cleaning １ Shut off regulator valve ② and then ① to stop CO supply. Turn Main Switch OFF. Remove top heater carefully and wipe dew water at the inside surface. Remove remaining water at water bath with using syringe. Remove chamber and lens heater from the microscope.
Page 36 Confirm gas is supplied to the controller from the regulator. Ensure that gas is delivered to the controller-side of tube. < STXG > Confirm there is no abnormal noises resulting from gas leakage. If the trouble cannot be solved, please contact Tokai Hit.
Page 37 Vessel to be used 35 mm petri dish The petri dishes of Iwaki, Nunc or Greiner have a cutout at the bottom and there is a possibility that the incubation environment cannot be kept stable or dew condensation is generated on the lens heater due to leakage. In a case of that, please use the exclusive spacer sold separately.
Page 38 If the display has not change then there information may be a potential for sensor disconnection. Please contact dealer or Tokai Hit. PV does not keep stable at Confirm the plug coming from chamber is connected SV after preliminary to controller properly.
Page 39 Status Guidance Glass breakage of Top Please contact dealer or Tokai Hit with serial number Heater of your device. Gas flow rate does not < STXG > increase enough. Confirm the CO function is on via PC or Touch Panel and leave it for about 20 minutes.
Page 40 7 Options (for STX-E upgrade) Optional① STX-FB: Sample Feedback regulation Sample Temperature Feedback Regulation allows to keep the optimal sample temperature from room temperature fluctuation. 1. Feedback actual media temp. to Controller 2. Controller regulate main heater based on the feedback signal *This feature can be unlocked after purchase by optional Pass word and USB memory.
Page 41 7 Options (for STX-E upgrade) Optional③ common add-on options. Following options can be purchased individually. Please contact Tokai Hit for more information. STX-CSG Dish Holder set for chamber slide, Slide glass and chambered coverglass ATX-D35-2-SET Dish Holder set for 35mm dish (2pc) UNIV2-SG4 Holder for 4pc slide glass.
Page 44 Tokai Hit Customer Support Please contact our support team with following information. 1. Description of inquiry or problem - Inquiry of regarding usage, - Details of malfunction or defect - Request of additional accessories and optional products 2. Serial Number - Six digit number at chamber cable and controller Email：...

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