Sutter Instrument BV-10 Operation Manual
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Sutter Instrument BV-10 Operation Manual

Micropipette beveler
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BV-10
Micropipette Beveler
Operation Manual
Rev. 3.00 ( 20180423)
One Digital Drive
Novato, CA 94949
Voice: 415-883-0128 Web: www.sutter.com
Fax:
415-883-0572 Email: info@sutter.com

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Summary of Contents for Sutter Instrument BV-10

  • Page 1 BV-10 Micropipette Beveler Operation Manual Rev. 3.00 ( 20180423) One Digital Drive Novato, CA 94949 Voice: 415-883-0128 Web: www.sutter.com Fax: 415-883-0572 Email: info@sutter.com...
  • Page 2 Copyright © 2018 Sutter Instrument Company. All Rights Reserved. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 5: Disclaimer

    SAFETY WARNINGS AND PRECAUTIONS Electrical  Operate the BV-10 using 110-120 V AC, 60 Hz, or 220-240 V AC., 50 Hz line voltage. This instrument is designed for connection to a standard laboratory power outlet (Overvoltage Category II). ...

  • Page 6: Operational

    This unit is designed for operation in a laboratory environment (Pollution Degree I).  This unit is designed for connection to a standard laboratory power outlet (overvoltage Category II) with main supply voltage fluctuations not to exceed ± 10% of the normal voltage. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 7: Table Of Contents

    5.4 No Beveling Occurs......................... 25 5.5 Meter Does Not Work ........................25 5.6 Pipettes Break During Beveling ....................25 APPENDIX A. LIMITED WARRANTY .................... 27 APPENDIX B. ACCESSORIES ......................29 Accessories ............................. 29 Replacement Parts ..........................29 BV-10 OPERATION MANUAL – REV. 3.00 (20180423)
  • Page 8 Figure 1-1. Manipulator..........................9 Figure 1-2. Pedestal/Grinding Assembly....................10 Figure 1-3. BVM-CE Electrode Resistance Meter................. 11 Figure 2-1. BV-10 beveler component layout..................13 Figure 2-2. Pedestal/grinding plate assembly..................15 Figure 2-3. Components of optical attachment..................16 Figure 3-1.

  • Page 9: General

    The BV-10 beveler design provides a flat, wobble-free abrasive surface for beveling intracellular electrodes, microinjection needles, etc. The BV-10 is suitable for beveling micropipettes with tip diameters between 0.1 and 50.0 microns (µ) or micrometers (µm), depending on the grinding plate installed. The grinding plate rotates on a stationary, optically flat pedestal by virtue of a magnetic coupling with a rotating drive ring.

  • Page 10: Features

    Includes micromanipulator, reference wick, reference wick holder, pedestal oil, degreaser, manual, and two abrasive plates of your choice. Previous versions of the BV-10-D and BV-10-E were equipped with an 80X stereomicroscope. The replacement 40X magnification provides improved location and adjustment for the field of view around micropipette tips while maintaining good magnification. If an 80X is preferred over the 40X, please contact Sutter Instrument for information.

  • Page 11: Manipulator

    It is factory pre-set to 1 (mid- point on the scale) for average beveling. Moving the pointer towards 0 will reduce the micrometer advance rate. Figure 1-1. Manipulator. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 12: Pedestal/Grinding Assembly

    The grinding plate rotates wobble-free on the flat quartz surface by virtue of an oil film bearing and a magnetic coupling between the retaining ring assembly and the drive ring. Figure 1-2. Pedestal/Grinding Assembly. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 13: Bvm-Ce Electrode Impedance Meter (Optional)

    1.5.4 BVM-CE Electrode Impedance Meter (Optional) The BVM-CE is an electrode resistance meter designed to complement the BV-10 electrode beveler. Resistance is indicated by a precision analog meter, which allows the user to easily identify changes in microelectrode resistance while beveling.

  • Page 14: Optical Attachment (Optional)

    To aid viewing the beveling process, an optical attachment is available. The unit consists of a 40x stereomicroscope and mounting hardware. For large diameter tips (>1 µm) the attachment allows the user to bevel using an optically based beveling technique described in subsequent text. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 15: Installation

    9. Optical attachment (stereoscopic microscope, if ordered (Models D and E)) Box 2 Micromanipulator (inside Box 1): Figure 2-1. BV-10 beveler component layout. NOTE: The BV-10 Impedance Meter (BV-10M or BVM-CE), if used, sits on top of the main BV-10 control unit. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 16: Set-Up And Assembly Instructions

    6. Assemble grinding plate system as described below. See Figure 2-2. Two grinding plates are supplied with the BV-10. The plates are made by embedding the abrasive particles on a film of plastic. With care, these plates can bevel thousands of pipettes.

  • Page 17: Figure 2-2. Pedestal/Grinding Plate Assembly

    7. Assemble optical attachment if supplied. — See Figure 2-2. a. Screw the vertical mounting post into the threaded hole in the BV-10 base plate. The Allen head bolt fits through the center of the mounting post and is used to screw the post onto the base plate.

  • Page 18: Figure 2-3. Components Of Optical Attachment

    Allen head bolt washer black mounting block with 2 thumb screws This completes the instructions for beveler assembly. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 19: Operation

    For bubble-beveling, distilled water, containing Photo-Flo (a 1% solution) is sufficient. The Photo-Flo is important for keeping the film of water as thin as possible and, by reducing surface tension, minimizing the tendency of the water to 'drag' the pipette tip. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 20: Control Of Beveling By Measuring Electrical Resistance

    Bubble beveling is a technique in which a gas (compressed air, nitrogen) is passed through the pipette to be beveled while the tip of the pipette is immersed in a thin film of water BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 21: Using The Resistance Meter

    The meter is wired for operation at 110 volts, unless it has been modified for operation at 220 volts. The proper voltage for your unit should be indicated on the back. If you need to operate BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 22: Operation

    +/- 1 minor division. This level of absolute accuracy is more than enough for use in micropipette work since the micropipette is far from being an ideal resistor that obeys Ohm's Law. In practice, the BV-10 OPERATION MANUAL – REV. 3.00 (20180423)
  • Page 23 It follows that an electrode resistance meter need only have absolute accuracy to within a few percent to accommodate most users. However, it is important to measure resistance with high resolution, particularly when screening electrodes or when beveling. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)
  • Page 24 (This page intentionally left blank.) BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 25: Maintenance

    CAUTION: Under no circumstances should the grinding plate be cleaned with any solvent except the degreaser recommended. Acetone or similar materials will immediately destroy the plate surface. Kimwipes ® is a registered trademark of Kimberly-Clark Corporation. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)
  • Page 26 (This page intentionally left blank.) BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 27: Troubleshooting

    ♦ If you are not able to bevel pipettes smoothly and/or pipettes seem to break or chip, the advancer should be adjusted for a slower advance rate per turn of the micrometer knob. BV-10 OPERATION MANUAL – REV. 3.00 (20180423)
  • Page 28 (This page intentionally left blank.) BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 29: Appendix A. Limited Warranty

    Warranty work will be performed only at the factory.  The cost of shipment both ways is paid for by Sutter Instrument during the first three months this warranty is in effect, after which the cost is the responsibility of the customer.
  • Page 30 (This page intentionally left blank.) BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 31: Appendix B. Accessories

    2-inch active lead (platinum to pipette) X050300 Reference wick M100019 Reference wick holder H906100 Drive belt O740130 Reticle grid for BV-10S scope, 5mm scale / 100 divisions PEDESTAL Pedestal plates (top and bottom) BV-10 OPERATION MANUAL – REV. 3.00 (20180423)
  • Page 32 (This page intentionally left blank.) BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 33: Appendix C. Fuse Replacement

    Replace the active fuse with the spare and re-install the fuse holder and power cord. If the controller fails to power up with the new fuse installed, call Sutter Instrument technical support personnel for assistance. Figure C-2. Fuse holder.
  • Page 34 Replace only with the same type and rating: Miniature Fuse, 5 x 20 mm, IEC 60127-2, 250VAC, Time-Lag T, Low Breaking Capacity (Glass Tube): At 110 - 120 VAC: Rating: 0.2A (200mA) Examples: Schurter 0034.3110 At 220 - 240 VAC: Rating: 0.1A (100mA) Examples: Schurter 0034.3107 BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 35: Appendix D. Technical Specifications

    0.075 in / dial revolution Fine drive: 0.0004 in / dial revolution Dimensions: 19 x 9 x 8 in (48 x 22 x 20 cm) Weight: 30 lbs (13.6 kg) Electrical: 110-120/200-240 volts, 50/60-Hz power line BV-10 OPERATION MANUAL – REV. 3.00 (20180423)
  • Page 36 (This page intentionally left blank.) BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

  • Page 37: Index

    ..............33 technical support ............. 8 Introduction ............. 7 troubleshooting beveling takes a long time ........ 25 mains grinding plate won't turn ......... 25 fuses ............... 3, 32 jerky plate ............25 BV-10 OPERATION MANUAL – REV. 3.00 (20180423)
  • Page 38 ......... 25 no beveling occurs ..........25 Unpacking and setting up ........13 pipettes break during beveling ......25 Troubleshooting ............ 25 warranty ..............27 NOTES BV-10 OPERATION MANUAL – REV. 3.00 (20180423)

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