Bio-Optica MNT Operating Instructions Manual
  1. Manuals
  2. Brands
  3. Bio-Optica Manuals
  4. Medical Equipment
  5. MNT
  6. Operating instructions manual

Bio-Optica MNT Operating Instructions Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
OPERATING
INSTRUCTIONS
INS2500GB
2020-03-020.00
Cryostat
MNT

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the MNT and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Summary of Contents for Bio-Optica MNT

  • Page 1 OPERATING INSTRUCTIONS Cryostat INS2500GB 2020-03-020.00...
  • Page 2 Operating Instructions – MNT 2020-03-020.00...

  • Page 3: Table Of Contents

    8. OPERATION OF MICROTOME ............22 9. OPERATION OF CRYOSTAT ............35 10. CLEANING AND MAINTENANCE ............40 11. SERVICE ............42 12. OPTIONAL ACCESSORIES ............43 13. WARRANTY ............45 14. DISPOSAL ............45 Operating Instructions – MNT 2020-03-020.00...

  • Page 4: Intended Use

    1. INTENDED USE The floor standing open top cryostat MNT is intended for rapid freezing and cutting thin sections of frozen specimens for professional use in routine and research laboratories in the fields of biology, medicine and industry. The system of knife advance operates very reliable from 0.5 to 100 µm with trimming function up to 750 µm.
  • Page 5 The hand wheel can be stopped in any position. For this purpose move the stop Hand wheel stop released. Movement. lever towards the centre. For releasing the stop please turn the lever towards the outside again. Hand wheel stop activated. No movement. Operating Instructions – MNT 2020-03-020.00...
  • Page 6 3.2 FINGER PROTECTION Use the finger protection always • Before you start any work with the knife or specimen • Before changing the specimen • During work break Finger protection released. Finger protection activated. Operating Instructions – MNT 2020-03-020.00...
  • Page 7 Never try to catch a falling microtome knife. Always insert the specimen first and then the microtome knife or disposable blade. To avoid compression or knife marks, ensure that the blade is clean. Operating Instructions – MNT 2020-03-020.00...
  • Page 8 Emergency stop switch. 3.6 MOTORIZED OPERATION For switching off the motor please always use the hand or foot switch. Do never simply set the speed to 0. Operating Instructions – MNT 2020-03-020.00...

  • Page 9: Components

    4. COMPONENTS The Bio-Optica open top cryostat MNT is provided with the following standard components: • Basic unit with refrigeration system • Fully automatic rotary microtome • Operation manual • Specimen holder (5x Ø 22mm; 5x Ø 35 mm) •...

  • Page 10: Specifications

    R 449 A, 50 g R 449 A, 50 g Cooling gas Chamber defrost hot gas defrost, time selectable, 1-3 times per day or Automatic defrost manual defrost on request at -5 °C chamber temperature Automatic stop Operating Instructions – MNT 2020-03-020.00...
  • Page 11 Dimensions (L x W x H) 135 kg (without object cooling) Weight (without accessories) 154 kg (with object cooling) *All temperature specifications refer to an ambient temperature of + 20 ° C and a relative humidity of 60% Operating Instructions – MNT 2020-03-020.00...

  • Page 12: Unpacking And Installation

    Extend the two front feet to stabilize the cryostat in its position by turning the supporting screws counter clockwise. For repacking use the original cases. Keep the packing material. Operating Instructions – MNT 2020-03-020.00...
  • Page 13 For opening the chamber push the window to the top. Remove the transport security underneath the specimen holder. After installation, wait at least 4 hours before switching the instrument on. Failure to comply may result in severe damage to the instrument. Operating Instructions – MNT 2020-03-020.00...
  • Page 14 We recommend filling the container with 500 ml disinfectant before initial operation. After installation, wait at least 4 hours before switching on the instrument. Failure to comply may result in severe damage to the instrument. Operating Instructions – MNT 2020-03-020.00...
  • Page 15 • For folding the lever back, pull the horizontally Hand wheel lever in folded position. orientated lever and fold it away. Hand wheel lever unfolded. Operating Instructions – MNT 2020-03-020.00...

  • Page 16: Initial Operation

    Software settings and motor operation can be operated with the control panel on the left and right side of the instrument. Software and horizontal motor control Cutting motor control panel panel on left top side of MNT. on right top side of MNT. Operating Instructions – MNT 2020-03-020.00...
  • Page 17 5 µm Change entries by pressing Start defrost Store new settings by pressing TUE 13:05:05 Press to return to the main menu. CUT: 7 µm Trim: 19µm Cha: 22 °C (25) QF: 0 Status:OK 0000 Operating Instructions – MNT 2020-03-020.00...
  • Page 18 Disinf. delay 10 h time for the automatic power saving mode. Incr. start 21:00 > (e.g. end at 04:00 h in this example) Incr. end 04:00 Incr. days _ _ _ _ _ 6 7 Operating Instructions – MNT 2020-03-020.00...
  • Page 19 Select the delay time at which the disinfection cycle 1 2 _ _ _ _ Disin. Days will be activated. > Disin. Delay 10 h (e.g. 10 hours) Incr. start 21:00 After confirmation the disinfection cycle will start at 10 p.m. Operating Instructions – MNT 2020-03-020.00...
  • Page 20 Light ON / OFF / AUTO The light can be set on or off. If AUTO has been selected, the light will switch off after the time being set under Light duration. Operating Instructions – MNT 2020-03-020.00...
  • Page 21 A language can be selected out of a list of available Language languages. Display version Display of software version. Upon selecting Program settings the automatic Program settings defrost and power saving settings can be changed. Operating Instructions – MNT 2020-03-020.00...

  • Page 22: Operation Of Microtome

    Adjust knife height by turning the wheels on the left and right side of the knife holder base. Release hand wheel lock for cutting. Loosening of knife fixation and insertion of new knife. Fixation and height adjustment of knife. Operating Instructions – MNT 2020-03-020.00...
  • Page 23 Loosen the blade fixation by turning the right lever counter clockwise. Remove/Insert blade from one side. Tighten the blade fixation by turning the right lever clockwise. Loosening of blade fixation and insertion of new blade. Fixation of blade. Operating Instructions – MNT 2020-03-020.00...
  • Page 24 Tighten the blade holder fixation by turning the right lever on the blade holder base clockwise. Release hand wheel lock for cutting. Fixation of blade holder with increased cutting angle. Operating Instructions – MNT 2020-03-020.00...
  • Page 25 4. Tighten the object holder by turning the fixation lever clockwise. The fixation lever is limited in the range of rotation downwards due to risk of collision. Release of object holder fixation; removal and/or insertion of object holder. Operating Instructions – MNT 2020-03-020.00...
  • Page 26 Release the handwheel lock to cut the samples. The object orientation has a zero point notch and automatically locks the sample holder parallel to the microtome. Fix the sample orientation. Operating Instructions – MNT 2020-03-020.00...
  • Page 27 Cha: -20 °C (-22) QF: 0 Activation of trim latching is displayed as illustrated. Status:OK 0000 If Trim latching mode is deactivated, trimming function is activated by continuously pressing Turn hand wheel evenly clockwise Operating Instructions – MNT 2020-03-020.00...
  • Page 28 Leave hand wheel with handle at its lowest point (specimen holder underneath the microtome knife or disposable blade). Transfer the section onto a slide. Leave brush always in the chamber to keep it cold. Operating Instructions – MNT 2020-03-020.00...
  • Page 29 To adjust the setting for the upward movement, press TUE 13:05:05 CUT: 7 µm Trim: 19µm and simultaneously to increase or Cha: -20 °C (-22) QF: 0 decrease the speed setting. ↓ 40 mm/s ↑ 140 mm/s Operating Instructions – MNT 2020-03-020.00...
  • Page 30 Do not start motor functions with by illumination of the LEDs as displayed above. activated hand wheel lock ! Failure to comply may result in severe damage to the instrument. Foot switch for control of the cutting motor. Operating Instructions – MNT 2020-03-020.00...
  • Page 31 Display with deactivated trimming function. Optionally press continuously to increase the cutting speed to the value set for the upward movement (see 8.12). If is released, the instrument will return to its original cutting speed. Operating Instructions – MNT 2020-03-020.00...
  • Page 32 Do not start motor functions with activated hand wheel lock ! Failure Foot switch for control of the cutting motor. to comply may result in severe damage to the instrument. Operating Instructions – MNT 2020-03-020.00...
  • Page 33 Horizontal adjustment of anti-roll plate For height adjustment of the anti-roll-plate it is recommended to adjust the correct height during cutting. Operating Instructions – MNT 2020-03-020.00...
  • Page 34 -13 to -20 Scrapings -16 to -25 Skin with fat -16 to -25 Skin without fat -10 to -16 Spleen -7 to -10 Testicle -10 to -13 Tongue -13 to -20 Uterine-Curettings -7 to -10 Operating Instructions – MNT 2020-03-020.00...

  • Page 35: Operation Of Cryostat

    UV light without the addition of chemicals. The cryostat MNT can be equipped with an UV-C light source that can either be automatically activated (see section 7.3) or manually activated.
  • Page 36 Picture 6: Picture 7: Once the plug is connected to the UV- Lamp, the final step can be done now and the UV-Lamp unit can clip into the retaining clips. Operating Instructions – MNT 2020-03-020.00...
  • Page 37 9.2 QUICK FREEZE POSITION [only applicable to instruments equipped with this feature] For convenience of the user, the cryostat MNT can be equipped with a quick freeze shelf with two positions located in the object freezing shelf. Upon activation the installed peltier element will cool down the two quick freeze position to approximately -55 °C.
  • Page 38 Before switching on the instrument ensure that the instrument is completely dry, otherwise ice built up can damage mechanical parts During defrosting (automatic or manual) always leave front window open. Operating Instructions – MNT 2020-03-020.00...
  • Page 39 The contents of the condensate bottle should be disposed according to laboratory regulations. To reduce the risk of contamination and infection, after emptying, is recommended to fill a disinfectant concentrate into the condensate bottle.. Operating Instructions – MNT 2020-03-020.00...

  • Page 40: Cleaning And Maintenance

    Monthly Check of temperature sensor in cryochamber. Cleaning of cryostat ventilation (see section 10.4). Yearly Complete Service (performed by authorized Bio-Optica service technician) • check of all functions • complete defrost • removing of microtome • oiling of movable parts •...
  • Page 41 Cleaning of disposable blade holder. Reassemble the pressure plate and the lever and loosen the locking device for cutting. Assemble the blade holder. Operating Instructions – MNT 2020-03-020.00...

  • Page 42: Service

    It must be returned in its original packing. If the instrument or parts thereof are sent back in a dirty or non-disinfected condition, Bio-Optica reserves the right to return the parts to the debit of the customer. Operating Instructions – MNT...

  • Page 43: Optional Accessories

    Object holder (Ø 22 mm) #30000002 Object holder (Ø 28 mm) #30000017 Object holder (Ø 35 mm) #30000000 Object holder (Ø 50 mm) #30000003 Clear-cut brush #30001030 Anti-roll plate (disposable blade holder) #32001050 Anti-roll plate (standard knife holder) #32001049 Operating Instructions – MNT 2020-03-020.00...

  • Page 44: Warranty

    13. WARRANTY Bio-Optica S.p.A guarantees that the product delivered has been subjected to a comprehensive quality control procedure, and that the product is faultless and complies with all technical specifications and/or agreed characteristics warranted. Bio-Optica S.p.A guarantees that the instrument is manufactured under an ISO 13485:2016 quality management system.
  • Page 46 Bio-Optica S.p.A. Via San Faustino 58 20134 Milano / Italy Tel. +(39) 02 2127 131 Fax +(39) 02 2153 000 info@bio-optica.it https://www.bio-optica.it...

Table of Contents