Analytik Jena UVP Hybridizer Operating Instructions Manual

Hybridization ovens

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UVP Hybridizer, UVP HybriLinker and UVP

Multidizer Hybridization Ovens

Operating Instructions

UVP Hybridizer

UVP Multidizer

UVP HybriLinker

IMPORTANT: To familiarize yourself with the correct operation procedures, read these instructions

before operating your UVP Hybridizer, UVP Multidizer or UVP HybriLinker system.

_________________________________________________________________________

Analytik Jena US

Ultra-Violet Products Ltd.

2066 W. 11th Street, Upland, CA 91786

Unit 1, Trinity Hall Farm Estate

Tel: (909) 946-3197

Nuffield Road, Cambridge CB4

Fax: (909) 946-3597

1TG UK

Tel: +44(0)1223-420022 Fax:

Web Site:

www.us.analytik.jena.com

+44(0)1223-420561

81-0169-01 Rev W

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Summary of Contents for Analytik Jena UVP Hybridizer

Page 1 UVP Hybridizer UVP Multidizer UVP HybriLinker IMPORTANT: To familiarize yourself with the correct operation procedures, read these instructions before operating your UVP Hybridizer, UVP Multidizer or UVP HybriLinker system. _________________________________________________________________________ Analytik Jena US Ultra-Violet Products Ltd. 2066 W. 11th Street, Upland, CA 91786...
Page 2: Table Of Contents
UVP Hybridization Ovens Table of Contents Table of Contents ......................2 Introduction ......................... 3 Hybridization Oven Specifications ................4 Setup Instructions ....................... 5 Operating Instructions ....................7 Care and Cleaning ..................... 12 Replacement Parts ....................13 Accessories ......................... 14 Maintenance, Repair and Technical Assistance ............16 Hybridization Techniques ..................
Page 3: Introduction
UVP Hybridization Ovens Introduction The UVP Hybridizer is a cost-effective, high-performance, 10-bottle hybridization oven with state-of-the-art microprocessor temperature and variable speed controls. The UVP HybriLinker™ System is designed with a combination of hybridizer and crosslinker components. This equipment is intended to be used in the laboratory environment.
Page 4: Hybridization Oven Specifications
UVP Hybridization Ovens Hybridization Oven Specifications UVP Hybridizer Hybridization Oven Part Numbers Volts/Hz 95-0030-01 115V/60Hz 95-0030-02 230V/50Hz 95-0030-03 100V/50Hz Specifications Net Weight: 43 lbs Temperature: Ambient +10°C to 99.9°C Temperature Stability: Inside Oven: +/-0.3°C to 68°C Inside Bottles: +/-0.1°C to 68°C Temperature Uniformity: Inside Oven: +/-0.1°C to 68°C...
Page 5: Setup Instructions
Carousel Rotation: 12 - 20 rpm Lower Chamber Same as the UVP Hybridizer Setup Instructions Two different bottle sizes are available for use within the hybridizer. The rotary wheel carousel can hold up to twenty (20) 35 x 150 mm bottles or ten (10) 35 x 300 mm bottles. In addition, combinations of both bottle sizes may be used at the same time.
Page 6 UVP Hybridization Ovens WheelS Fig. 1 Inserting Bottle Holder Carousel Fig. 2 Adjusting for Thumb Bottle Lengths Screw Fig. 2 Adjusting for bottle lengths Fig. 3 Inserting Bottles Fig. 4 Offset/Skewed Bottle Positioning Page 6 81-0169-01 Rev W...
Page 7: Operating Instructions
UVP Hybridization Ovens Installing the Rollers and Optional Trays To insert rollers in the hybridization oven carousel, slide the rollers into the receptacle on the left side of the chamber first, followed by the right side. To install the Reciprocating Shaker Tray (standard with the Multidizer), place the tray over the rollers in the hybridization oven.
Page 8 Calibrating the Temperature Reading The hybridizer is calibrated at the Analytik Jena factory. AJ recommends temperature recalibration be performed at the AJ factory as recalibration by the user may void the warranty. Obtain an RGA (Returned Goods Authorization) number from AJ prior to returning a unit to the factory. Call AJ’s customer service department in the USA in Upland, California at (909) 946-3197 or in the UK in Cambridge, UK at +44(0)1223420022.
Page 9 UVP Hybridization Ovens Oven Chamber The oven chamber and drip pans may be decontaminated by wiping clean with a decontaminating agent, then repeat with distilled water. NOTE: If any unit requires service, a Returned Goods Authorization (RGA) number must be obtained from AJ’s customer service department prior to returning any item to AJ.
Page 10 UVP Hybridization Ovens Set your new preset UV exposure by pressing the numbers on the touchpad. Remember: The number indicated on the LCD display must be multiplied by 100 to determine the proper exposure setting. The new setting will then appear on the display. Press “ENTER”...
Page 11 The Crosslinker is shipped with shortwave UV tubes. However, should user requirements and applications change, it is possible to modify the ultraviolet wavelength and recalibrate the UV sensor and microprocessor to the new UV wavelength. This is accomplished by purchasing FIVE tubes of the new UV wavelength and the proper Analytik Jena calibration sensor.
Page 12: Care And Cleaning
UVP Hybridization Ovens Care and Cleaning NOTE: Always unplug the unit from the electrical supply before cleaning or drying the unit. The units are built to provide you with trouble-free operation. To ensure correct operation:  Wipe ANY water from inside and outside the unit with a soft cloth or sponge. ...
Page 13: Replacement Parts
UVP Hybridization Ovens Replacement Parts Replacement parts lists are provided below for the Hybridizer and HybriLinker systems. Repairs or replacement other than as specified in the following procedures should only be completed by authorized service personnel. Replacement Parts for Hybridizer DESCRIPTION QTY.
Page 14 UVP Hybridization Ovens Replacement Parts for Multidizer DESCRIPTION QTY. NEEDED PART NO. Borosilicate Glass Bottle, with Cap and O-Ring Large, 35 x 300mm 07-0194-02 Cap and O-Ring (for all Bottle sizes) 88-0004-01 Bottle Holder Carousel 76-0070-01 Carousel Drive Shaft Rod 76-0064-03 Drip Tray (for Lower Chamber) 10-0292-02...
Page 15: Accessories
UVP Hybridization Ovens Accessories Bottles: Borosilicate glass bottles with polypropylene cap and PTFE seal: Bottle, Small, 35 x 150 mm 07-0194-01 Bottle, Oversize Call AJ Bottle Carousels: Carousel Kit for Four Oversized Bottles 76-0066-02 Carousel for (10) 50ml Conical Bottles 76-0075-01 Carousel, Acrylic (for HM-4000 upper chamber) 76-0089-01...
Page 16: Maintenance, Repair And Technical Assistance
AJ’s duty under the warranty is limited to replacement and/or repair of the defective part at the option of Analytik Jena. AJ shall not be held liable for any expenses or damages incurred by the purchaser except expressly set forth herein, and in no event shall AJ be liable for any special, incidental or consequential damages of any kind.
Page 17: Hybridization Techniques
UVP Hybridization Ovens Hybridization Techniques There are really two main steps to a hybridization reaction: hybridizing two strands of complementary DNA, and detection of the hybridized DNA. Nucleic acid hybridization is a mechanism where strands of DNA in a single stranded state have their complements bind together.
Page 18 UVP Hybridization Ovens 3. Salt Concentration and Temperature Nucleic acid requires salt (monovalent cations) to reduce the ionic effects of the phosphate backbone, and heat as a form of non-denaturing kinetic energy. Because the salt concentration and temperature effect each other, knowing the thermostability of the hybrid probe is helpful. Hybridization rate varies directly with the sodium ion concentration between 0.03 and 1.2 M.
Page 19 UVP Hybridization Ovens Protocol 1: Random Priming Method for Tagging DNA with Fluorescein-Labeled Nucleotide and Others This method uses DNA polymerase to incorporate Fluorescene-11- dUTP into double stranded DNA probes. This protocol can be used to incorporate any tagged nucleotides. Equipment ...
Page 20 Incubate with mixing at 40-50°C for 2 hours or until dissolved. Store at 20°C. SDS will precipitate at room temperature but remain in solution at 37°C.  Analytik Jena Hybridization bottle(s) and caps  15 mL plastic tube ...
Page 21 UVP Hybridization Ovens Wash filter in 0.15X SSC, 0.1%SDS for 15 minutes at 50 °C (repeat once) Store blots in 2X SSC at room temperature Radioactive Probes Additionally you will need:  X-Ray film holder  X-Ray film  Intensifying screen Procedure Remove blot from hybridization tube and transfer to Tupperware container Rinse briefly in 50 °C 0.1X SSC, 0.1% SDS.
Page 22 Sambrook, J; Molecular cloning: a Laboratory manual; 1987, Cold Spring Harbor Laboratory Press; ISBN 0-87969-309-6 Current Protocols in Molecular Biology; 1987, John Wiley and Sons; ISBN 0-471-50338-X HybriLinker and Multidizer are trademarks of Analytik Jena. Page 22 81-0169-01 Rev W...

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