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Harvard Bioscience Multi Channel Systems SE Smart Ephys MEA Manual

Signal generator
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MEA Signal Generator
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Summary of Contents for Harvard Bioscience Multi Channel Systems SE Smart Ephys MEA

  • Page 1 MEA Signal Generator Manual...
  • Page 2 Imprint Information in this document is subject to change without notice. No part of this document may be reproduced or transmitted without the express written permission of Multi Channel Systems MCS GmbH. While every precaution has been taken in the preparation of this document, the publisher and the author assume no responsibility for errors or omissions, or for damages resulting from the use of information contained in this document or from the use of programs and source code that may accompany it.

  • Page 3: About This Manual

    1 Introduction 1.1 About this Manual This short manual is an addendum to the elaborate MEA Manual. It is strongly recommended to read the MEA manual first, if you utilize the MEA Signal Generator for introducing yourself to the MEA-System. Being already familiar with MEA-System, the MEA-SG will be easy to mount and to handle.

  • Page 4: Important Safety Advice

    2 Important Safety Advice Warning: Make sure to read the following advice prior to installation or use of the device and the software. If you do not fullfil all requirements stated below, this may lead to malfunctions or breakage of connected hardware, or even fatal injuries. Warning: Always obey the rules of local regulations and laws.

  • Page 5: Guarantee And Liability

    2.1 Guarantee and Liability The general conditions of sale and delivery of Multi Channel Systems MCS GmbH always apply. They can be found online at http://www.multichannelsystems.com/sites/multichannelsystems.com/files/documents/Terms and Conditions.pdf Multi Channel Systems MCS GmbH makes no guarantee as to the accuracy of any and all tests and data generated by the use of the device or the software.
  • Page 6 3 Welcome to the MEA Signal Generator The MEA Signal Generator is a convenient tool for MEA users. As a variable substitute for expensive biology you can use the MEA-SG as you would set up a research experiment. That means you can test your own products and data acquisition settings without using valuable biological samples.
  • Page 7 120MEA-SG, 256-MEA-SG, ME-W-SG The produces sine waves or real signals in digitized form. These signals are detected as analog signals with the MEA-System. Because of this data source you are able to use and to test the complete MEA-System. Each MEA-SG operates in a similar way to the standard MEA probe, but without a biological object. It is possible to simulate 12 different types of signals.
  • Page 8 Table: DIP switch position and corresponding signals Switch 1 Switch 2 Press Signal Source Signal Type button n times Artificial Sine Wave 0.005 Hz (The MEA- Note: sine waves <1 Hz might SG is ON) not be visible because of the hardware filter bandwidth.
  • Page 9 4 Setting up and Connecting the MEA Signal Generator A MEA-SG is handled in the same manner as a MEA probe for experiments. For detailed information please read the MEA Manual. Mounting instructions: Open the lid of the amplifier. Place the respective MEA-SG inside. Please orientate the chip as shown on the picture. Replace the lid and close it carefully.
  • Page 10 Short Circuit The amplitude of the sine waves in the MC_Rack data display above alternates in a range of 100 % and 50 %. Please consider the different levels of amplitudes in direct vicinity side by side regarding to the contact pads of the 60MEA-SG, but not to the electrode arrangement in the displays. If there is a shot circuit between neighbor pads of contact pads, all electrodes will show the same averaged amplitude.
  • Page 11 5 Multi Channel Suite Software With a MEA-SG it is easy to train users on the software Multi Channel Experimenter or MC_Rack, which are specially made for MEA2100-, MEA- and Wireless-Systems. Together with the MEA2100-System or an USB based data acquisition device and the software package you have a complete setup for data acquisition, and MEA-SG generates the data for training instead of an animal experiment.
  • Page 12 Please consider the rules for connecting the instruments. The Multi Channel Experimenter program allows only connections between output ports at the lower side of the device to the input port on the upper side of the target device. The input and output ports of the devices are color coded for a better assignment of the instruments.

  • Page 13: Getting Started

    6 MC_Rack In MC_Rack (the rack you use to record and analyze data online and offline) the data files are independent from each other. You can save and reuse a rack for several experiments and generate separate data files. You can then load the generated data file with another rack for further offline analysis later. The main power of MC_Rack is its great flexibility.
  • Page 14 Adding a Data Source 1. Click "Data Source Setup" in the MC_Rack main menu "Edit". 2 The "Channel Layout" dialog opens. 3. Select your data acquisition from the left "Data Source" drop down menu. 4. Add the data source "Add Data Source" from "Edit" menu or via icon Insert a Data Display 1.
  • Page 15 1. On the toolbar, click on the "Tape" icon to add a "Replayer" to your rack. The "Replayer" property sheet is opened. 2. Click the "Replay File" tab. Here, you load the file you like to review or to analyze offline. 3.

  • Page 16: Grounding And Shielding

    7 Troubleshooting the MEA Amplifier It is possible to control some functions of a MEA amplifier with a MEA-SG. Please read also chapter "Troubleshooting" in MEA Manual. Most of the problems with MEA-Systems are rare, and most are minor problems which can be easily avoided and solved. If a problem persists, please contact your retailer. With a MEA-SG you are able to check the electrode pins of your amplifier and the general behavior due to grounding and shielding the system.
  • Page 17 7.2 Checking the Electrode Pins of the Amplifier Noise in single Electrodes Starting 60MEA-SG with 12.5 Hz sinus waves you may see the following display: Channel 66, 67, 68 show a significant higher level of noise, channel 78 is completely in saturation. That means, there is a problem with the contact pad of the 60MEA-SG and / or the electrode pins of the amplifier.

  • Page 18: Scope Of Delivery

    8 Scope of Delivery MEA Signal Generator: 60MEA-SG or 256MEA-SG or 120MEA-SG or ME-W-SG Lithium Battery CR 1620 (durability maximal 2 years) With MEA-SG only: Grounding Cable CB-GND MEA Signal Generator Manual Batteries do not belong in normal household waste and, thus, must always disposed of within the framework of existing legislation.

  • Page 19: Contact Information

    9 Contact Information Local retailer Please see the list of official MCS distributors on the MCS web site. Mailing list If you have subscribed to the mailing list, you will be automatically informed about new software releases, upcoming events, and other news on the product line. You can subscribe to the list on the contact form of the MCS web site.

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