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3. Loading the Specimen
4. Starting Observation
(7) Ensure the chamber is at air, ie AIR LED is not
blinking and is WHITE.
If the EVAC LED is Blue, press the EVAC/AIR switch. The
AIR LED (WHITE) should start blinking. When the AIR
LED stops blinking and remains illuminated the
chamber is at AIR.
(8) After the AIR LED stops blinking wait approximately
five seconds or more before slowly drawing out the
specimen stage.
(9) Insert specimen stub assembly into the stage base.
(10) OPTIONAL Lightly tighten the hex screw to secure
the specimen stub assembly to the stage base.
(11) Use the X, Y knobs to centre the specimen stage
by lining up the centre position mark with the guides.
(12) Press the EVAC/AIR Switch to evacuate the
chamber. The EVAC LED will blink BLUE while the
chamber is being evacuated. Once evacuation is
complete (~2.5 min) the EVAC LED will stop blinking
and remain illuminated (BLUE)
(13) Select Accelerating Voltage / Observation condition.
(14) Select Vacuum level
(15) Select Detector. Can change selection later so not
critical to select at this point.
(16) Click the
(17) Use the auto functions
a) Set desired field of view using the X, Y knobs on the
front of the TM4000.
b) Adjust the Brightness and Contrast (click the Auto
B/C button.
c) Perform focusing (click the Auto Focus button)
a) Perform manual focus adjustment if
required.
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button to turn on the high voltage.
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