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Summary of Contents for Lighthouse ActiveCount100
- Page 1 ActiveCount100 M I C R O B I O L O G I C A L S A M P L E R 1000L 00:10:00 00:10:00 0.0 L 02/26/2015 USER001 12:45:30 PM LOC001 Operators Manual...
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- Page 3 Copyright © 2018 by Lighthouse Worldwide Solutions. All rights reserved. No part of this document may be reproduced by any means except as permitted in writing by Lighthouse Worldwide Solutions. The information contained herein constitutes valuable trade secrets of Lighthouse Worldwide Solutions.
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Page 4: Table Of Contents
Introduction ................................... 7 ActiveCount100 Basics .............................. 7 Safety Warnings ................................ 7 Touch Screen Calibration ............................8 Quick look at ActiveCount100 ........................... 9 Plug In USB Flash Drive and Charge Battery ......................10 Get Ready For Sampling ............................11 Interpret Results ................................12 Microorganism Sampling Principles ........................ - Page 5 Additional Help ................................ 35 Appendix A: Database Files............................36 Databases in Comma-Delimited Files ........................36 Location Database ..............................36 User Database ................................. 36 Sampling Run Databases ............................37 Appendix B: Feller Conversion Table .......................... 39 Sampling Head with 300 Holes x 0.6mm ......................... 39 Appendix C: Limited Warranty ............................
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Page 6: Eu Ce Declaration Of Conformity
EU CE DECLARATION OF CONFORMITY Manufacturer’s Name: Lighthouse Worldwide Solutions, Inc. Manufacturer’s Address: Lighthouse Worldwide Solutions, Inc. 1221 Disk Drive Medford, OR 97501 USA Declares the product: Product Name: ActiveCount Model Number(s): ActiveCount100 Conforms to the following Product Specifications: SAFETY... -
Page 7: Introduction
To minimize the chance of electric shock, turn off the instrument and disconnect power adapter during sterilization. Do not charge ActiveCount100 with a power supply other than one provided by Lighthouse Worldwide Solutions. Do not submerge ActiveCount100 in any liquids. -
Page 8: Touch Screen Calibration
Lighthouse ActiveCount100 Operating Manual Touch Screen Calibration The ActiveCount100H 3.5” color touch screen is calibrated prior to shipping and there should be no need to recalibrate at any time. Alternatively touch screen calibration may be performed at any time by following the steps outlined here. -
Page 9: Quick Look At Activecount100
Lighthouse ActiveCount100 Operating Manual Quick look at ActiveCount100 User-friendly and easy to use Status LED Large 3.5” (8.9 cm) color touchscreen Allen wrench to adjust petri dish holder Airflow 100 liters per min ± 4% Lithium ion rechargeable battery ... -
Page 10: Plug In Usb Flash Drive And Charge Battery
Plug In USB Flash Drive and Charge Battery Data logging. Insert the included USB flash drive to store sampling run metadata. Charge battery. Follow the instructions below to charge the ActiveCount100 battery. To achieve a fully charged battery, please allow four hours. -
Page 11: Get Ready For Sampling
Lighthouse ActiveCount100 Operating Manual Get Ready For Sampling Follow these steps to prepare Activecount100 for microorganism sampling. When ActiveCount100 is not in use, always cover sampling head with dust cover. 248083444-1 Rev 2 P a g e 11 of 40... -
Page 12: Interpret Results
MPN (most probable number) is a well established method to estimate the number of viable microorganisms in a monitored area. To derive MPN, ActiveCount100 uses the principle that statistically assumes an increasing number of organisms enter the same hole in the sampling head as the number of organisms per sample rises. -
Page 13: Constant And Periodic Sampling Modes
Description Sampling Mode Constant ActiveCount100 continuously samples until it reaches the desired sample volume at 100 liters per minute ± 4%. For the example below, select 1,000 liters of air as the desired volume. ActiveCount100 performs the following calculation to determine the sampling period is 10 minutes. - Page 14 ActiveCount100 determines ten one-minute sampling cycles are needed over two hours. 1,000 liters ÷ 100 liters/minute = 10 minutes Pause between cycles. ActiveCount100 calculates the number of minutes to pause between ten one-minute samples over two hours, or 12.22 minutes. 248083444-1 Rev 2...
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Page 15: Continuous Sampling Exercise
Lighthouse ActiveCount100 Operating Manual Continuous Sampling Exercise Select Constant mode: At the Home screen, press CONFIGURATION button. Select Constant in Mode menu item, then select total sampling volume. If desired, also select an optional delay time (in seconds). Press HOME button to return to the Home screen. -
Page 16: Periodic Sampling Exercise
Lighthouse ActiveCount100 Operating Manual Periodic Sampling Exercise Select Periodic mode: At the Home screen, press CONFIGURATION button. Select Periodic in Mode menu item, then select total sampling volume, delay time, total time to sample and the number of cycles. Press HOME button to return to the Home screen. -
Page 17: Security: Configuration Lock
Lighthouse ActiveCount100 Operating Manual Security: Configuration Lock ActiveCount100 Sampling Modes can be restricted by locking the Configuration Screen. Before locking the configuration screen; select the sample mode and sample settings to be locked. Configuration Lock: With the configuration screen locked; users are restricted to the preselected sample mode (Constant/Periodic/Gas). -
Page 18: Status Led Indicates Sampling Status
Service/Calibration Required Yellow Solid White LED Indicates Writing To USB Flash Drive: Do not remove USB flash drive and do not power off ActiveCount100 while LED is white and downloads are in progress. 248083444-1 Rev 2 P a g e... -
Page 19: Sampling Status Screens
Lighthouse ActiveCount100 Operating Manual Sampling Status Screens Display Sampling Status Screens: When a sampling run completes, ActiveCount100 displays a dialog box to indicate (a) success, (b) manual abort by user, or (c) flow error. (a) Success (b) Manual abort by user... -
Page 20: Total Sampling Volume
Modify Sampling Volume Preset Values: To modify a sampling volume value, press and hold then release a sampling volume button. ActiveCount100 then displays a keypad to enter a new sampling volume value. The new value then becomes the active sampling volume. -
Page 21: Locations And Users
Locations Database: ActiveCount100 stores up to 400 locations in its Locations database. When ActiveCount100 completes a sampling run, it saves the active location in a metadata record for the most recent sampling run. Each location can consist of up to 16 characters. -
Page 22: Assigns Active User To Sampling Runs
Lighthouse ActiveCount100 Operating Manual Assigns Active User to Sampling Runs Users Database: ActiveCount100 stores up to 50 users. When ActiveCount100 completes a sampling run, it saves the active user in a metadata record for the most recent sampling run. Each user name can consist of up to 16 characters. -
Page 23: Data Logging - Sampling Metadata
Data Logging – Sampling Metadata Save Sampling Metadata to USB Flash Drive No USB Flash Drive: If no USB flash drive is plugged into the rear USB port, ActiveCount100 stores metadata only for the most recent run in volatile memory, which ActiveCount100 deletes when ActiveCount100 is turned off. -
Page 24: Status Indicators
Status Indicators Device Status Indicators Status Indicators: ActiveCount100 displays status indicators on the Home screen. If ActiveCount100 displays Device status flag, it may indicate a device issue or problem. To display more device status information, press SETTINGS button, then press the Status menu item. - Page 25 Sampling Volume Good flow Flow status while sampling. Flow error If a flow error condition persists, contact Lighthouse Service and Support. See Section Technical Specifications for contact information. Device status flag Possible device error, e.g., flow error, calibration due, etc. To display the device status, press SETTINGS button and Status menu item.
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Page 26: Settings
Lighthouse ActiveCount100 Operating Manual Settings ActiveCount100 Settings Device Settings: Press SETTINGS button to display and edit device settings. 248083444-1 Rev 2 P a g e 26 of 40... -
Page 27: Tripod/Monopod
Vertical and Horizontal Sampling Vertical Sampling: Place ActiveCount100 on a horizontal surface to sample vertically. Horizontal Sampling: Mount ActiveCount100 on a tripod/monopod and turn the instrument to sample horizontally. ActiveCount100 supports 3/8” tripod/monopod screws and ¼” tripod/monopod screws using the included adapter. -
Page 28: Optional Compressed Gas Sampler
Lighthouse ActiveCount100 Operating Manual Optional Compressed Gas Sampler Verify Nominal Flow Before Gas Sampling Before using the optional compressed gas sampler, first verify nominal flow of ActiveCount100 without the gas sampler. Add empty petri dish as a place holder during Nominal Flow Rate verification. - Page 29 Lighthouse ActiveCount100 Operating Manual To verify ActiveCount100 nominal flow rate, follow the steps below. When ActiveCount100 displays the following screen, position gas sampler snugly in the sampling head while ActiveCount100 blower is running. 248083444-1 Rev 2 P a g e...
- Page 30 Adjust the gas sampler valve until the arrow in the display screen points straight up for nominal flow. ActiveCount100 and the Gas Sampler adapter are now ready to sample compressed gas. Press the BACK button to configure your gas sample.
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Page 31: Clean And Disinfect
Avoid Cross Contamination Sterilize ActiveCount100 after each cycle to avoid cross-contamination, which can adversely affect reliability of microbial samples. Always wear a pair of sterile gloves when sterilizing ActiveCount100. Sanitize gloved hands first, with a disinfectant solution. Use a commercial disinfectant solution, such as 70% - 90% ethanol solution or 60% - 80% isopropanol/water solution. -
Page 32: Warnings
Do not submerge ActiveCount100 in any liquids. Do not spray disinfectant solution directly into ActiveCount100 enclosure. Do not use gases to disinfect ActiveCount100. Lighthouse will void the warranty if an unsuitable disinfectant solution damages ActiveCount100. -
Page 33: Calibration
Lighthouse ActiveCount100 Operating Manual Calibration Calibration – Contact Authorized Facility ActiveCount100 is factory calibrated to 100 liters/min ± 4%. Contact a factory authorized facility to calibrate ActiveCount100 annually. To display calibration due date, see Section Settings. 248083444-1 Rev 2 P a g e... -
Page 34: Technical Specifications
Lighthouse ActiveCount100 Operating Manual Technical Specifications Benefits Compact design User-friendly and easy to use Reliable measurements Low cost of ownership Includes calibration certificate Features Uses standard ISO 24998 90mm (85mm to 91mm) petri dishes ... -
Page 35: Package Contents
Lighthouse ActiveCount100 Operating Manual Package Contents ActiveCount100 with aluminum sampling head, dust cover USB flash drive 24V 3A DC power adapter Allen wrench to adjust petri dish holder Tripod/monopod adapter Calibration certificate Operating Manual (this document) supplied on USB flash drive ... -
Page 36: Appendix A: Database Files
Lighthouse ActiveCount100 Operating Manual Appendix A: Database Files Databases in Comma-Delimited Files ActiveCount100 stores data in comma-delimited files on a USB flash drive in folder: /ActiveCount100_<serial-number>/ where <serial-number> is the ActiveCount100 serial number. To display the serial number, see Section Settings. -
Page 37: Sampling Run Databases
“Ray Lopez” . . . Sampling Run Databases Sampling Metadata in CSV Files: ActiveCount100 stores sampling metadata in the following files: /Active Count 100_<serial-number>/acsamples_<date>.csv Where <date> is today’s date, e.g., YYYY-MM-DD. ActiveCount100 appends one line of sampling metadata for each sampling run. - Page 38 ActiveCount100 serial number 0123456789 Flow Rate ActiveCount100 flow rate 100 L/m Last Calibration ActiveCount100 date last calibration 1/1/2016 Calibration Due ActiveCount100 date for next calibration 1/1/2017 Firmware 1 ActiveCount100 display firmware version 0.01.004 Firmware 2 ActiveCount100 power firmware version 0.0.002 Firmware 3 ActiveCount100 graphics firmware version 1.0.006...
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Page 39: Appendix B: Feller Conversion Table
R = number of Colony Forming Units (CFU) counted on 90 mm petri dish agar after incubation = Most Probable Number (MPN) of microorganisms in volume of air sampled N = number of holes in ActiveCount100 sampling head (300) 1006 1036 1069... -
Page 40: Appendix C: Limited Warranty
Limitation of Warranties Lighthouse Worldwide Solutions (LWS) warrants that all equipment shall be free from defects in material and workmanship under normal use for a period of two years from date of shipment to Buyer. LWS does not warrant that operation of the software will be completely uninterrupted or error free or that all program errors will be corrected. - Page 41 Service and Support Tel. 1-800-945-5905 (USA Toll Free) Tel. 1-541-770-5905 (Outside of USA) techsupport@golighthouse.com www.golighthouse.com...
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