Table of Contents
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INSTRUCTION MANUAL
ZymoPURE
Catalog Nos. D4209, D4210, D4211 & D4212
Highlights
•
Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.
•
Innovative ZymoPURE
as 25 µl.
For Research Use Only
Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com
™
Plasmid Miniprep Kit
™
technology enables elution of ultra-pure endotoxin-free plasmid DNA in as little
Contents
Product Contents ................................................. 1
Product Specifications .......................................... 1
Product Description .............................................. 2
Procedure Overview ............................................. 3
Buffer Preparation ................................................ 4
Protocol ............................................................. 4-6
Troubleshooting ................................................... 7
Ordering Information ............................................ 8
Related Products............................................. 9-10
ZYMO RESEARCH CORP.
(Patent Pending)
Version 1.0.0
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Table of Contents
Summary of Contents for Zymo Research ZymoPURE Plasmid Miniprep
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Page 1: Table Of Contents
Buffer Preparation ..........4 Protocol ............. 4-6 Troubleshooting ........... 7 Ordering Information ..........8 Related Products..........9-10 For Research Use Only Version 1.0.0 ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com... -
Page 2: Product Contents
• Recovery Volume: ≥ 25 µl of ZymoPURE ™ Elution Buffer or DNase free water ™ Trademarks of Zymo Research • Required Equipment: Microcentrifuge and/or vacuum manifold (recommended). Corporation. ™ • Processing Time: 15 min... -
Page 3: Product Description
Plasmid DNA (pGL3®) was isolated from 5 ml of JM109 E. coli culture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research). ZYMO RESEARCH CORP. -
Page 4: Procedure Overview
Page 3 Procedure Overview: ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com... -
Page 5: Buffer Preparation
To continue processing the lysate using the recommended vacuum protocol, proceed to the next page. If a vacuum is not available, proceed to page 6 for an alternative centrifugation method. ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com... - Page 6 ™ Zymo PURE Elution Buffer to 50 ºC and/or increasing the incubation period up to 5 minutes prior to centrifugation. ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com...
- Page 7 ™ Zymo PURE Elution Buffer to 50 ºC and/or increasing the incubation period up to 5 minutes prior to centrifugation. ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com...
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Page 8: Troubleshooting
DNA contamination in your sample. • Overgrown culture. Overgrown or old cultures may contain more genomic DNA contamination than fresh cultures. ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com... -
Page 9: Ordering Information
™ ZymoPURE Elution Buffer 12 ml D4200-7-12 30 ml D4200-7-30 C1055-10 ™ Zymo-Spin II-P C1055-50 C1001-50 Collection Tubes C1001-500 1000 C1001-1000 ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com... -
Page 10: Related Products
Page 9 ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com... - Page 11 Page 10 ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com...
- Page 12 ZYMO RESEARCH CORP. Phone: (949) 679-1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ info@zymoresearch.com ▪ www.zymoresearch.com...
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