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SDI Synbiosis ProtoCol 3 Quick Manual

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10
Identify zones
Sample
Sample
Sample
11
Review zone
measurements
Click
12
Measure
Click
Click
Click
ProtoCOL 3
26
From Image click on an area of
the disc or well to pick colour,
repeat for zone and the
background. Be sure to take
representative samples
from across the plate.
Click Next.
If all zones are detected click
Finish, if not repeat step 8
and ensure all of the
zones are central.
Select Measure tab.
Test Measure Plate.
Assign a plate ID.
Accept batch.
is now ready to make the
first measurement
Synbiosis Europe and International Headquarters:
Beacon House Nuffield Road Cambridge CB4 1TF UK
Tel: +44 (0)1223 727125 Fax: +44 (0)1223 727101
email: support@synbiosis.com
Synbiosis USA Headquarters:
5123 Pegasus Court Suite Q Frederick MD 21704 USA
Tel: 800-686-4451/301-662-2863
email: support@synbiosis.com
Website: www.synbiosis.com
All trademarks acknowledged A0029.06.19
SYNBIOSIS
PROTOCOL 3
QUICK GUIDE

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Summary of Contents for SDI Synbiosis ProtoCol 3

  • Page 1 Identify zones SYNBIOSIS From Image click on an area of the disc or well to pick colour, Sample repeat for zone and the PROTOCOL 3 background. Be sure to take Sample representative samples from across the plate. QUICK GUIDE Sample Click Next.

  • Page 2: Table Of Contents

    CONTENTS GETTING STARTED GETTING STARTED Thank you for ordering your Synbiosis ProtoCOL 3 automated CONNECTING YOUR TOUCH SCREEN PC colony counter and zone sizing system. SOFTWARE INSTALLATION If you ordered your device with a touchscreen PC, please follow from HOW TO ADD A USER...

  • Page 3: Connecting Your Touch Screen Pc

    CONNECTING YOUR TOUCH SCREEN PC Unpack ProtoCOL 3 Attach leads to PC and monitor Unpack the instrument and the PC and place on a secure level surface. Attach the leads to the PC and Attach bracket and to the monitor before securing PC to ProtoCOL 3 the monitor onto the bracket (step 5).

  • Page 4: Software Installation

    SOFTWARE INSTALLATION Start PC Attach power cords to Log onto PC as an the mains power blocks Administrator and shut all running software down. Insert the Synbiosis USB flash drive Browse to the flash drive in There are 2 power leads found Windows named ‘Software’.
  • Page 5 Connect the ProtoCOL 3 Connect the power cord to the ProtoCOL 3 and plug the unit in. Connect the camera USB cable from the ProtoCOL 3 unit to a Press Next or select spare USB port in the PC. an alternative install location.
  • Page 6 Press Install. Press Finish. Press Finish. Press Next. Press Next. Press Finish. The Database Updater application will appear. If this box disappears after a few seconds then skip to step 25. If the box remains Press Install. on display then this means the database has not been created.
  • Page 7 Software Key The data paths window Activation Method will appear. Enter a name for the database e.g. (ProtoCOL DB). Press the New Database button (on the right hand side of the Database Name). You will see a Enter the media key message saying provided in your “Database Successfully...
  • Page 8 Hardware Key Run the calibration Activation Method installer Browse to the Synbiosis USB flash drive in Windows and open the “Calibration” folder. Select the USB key. Run the “SetupCalibration” Click Next. program and then follow the on screen instructions. Add Users to user groups Remove all connected USB dongles from the HOW TO ADD A USER (page 14).

  • Page 9: How To Add A User

    HOW TO Accessing the User Groups ADD A USER When ProtoCOL 3 is installed, three default user groups are created: ProtoCOL Admins ProtoCOL Advanced Users ProtoCOL Users Every person using this device needs to be a member of one of these groups Expand Local Users and Groups and click on Group folder.
  • Page 10 Add a Single User Add a Group of Users (Cont) Click Add. Select the group To add a single you want user, enter to add and click OK. their Windows username in the box labelled “Enter the object names to select” and click Check Names.

  • Page 11: Count Validation Procedure

    COUNT VALIDATION PROCEDURE Pour Plate Validation Procedure The purpose of this validation is to confirm that the ProtoCOL 3 system correctly uses contrast and background to Insert the pour plate validation plate into the ProtoCOL 3 and close the doors to minimise ambient light. accurately count a set number of entities in a known area.

  • Page 12: Zone Validation Procedure

    ZONE VALIDATION PROCEDURE Zone Validation Procedure The purpose of this validation is to confirm that the ProtoCOL 3 system Insert the zone measurement validation plate into the ProtoCOL 3. correctly uses contrast and background 2. Log on to the system, and then log onto the software. to accurately measure 3.

  • Page 13: Total Plate Count Batch Set-Up

    TOTAL PLATE COUNT BATCH SET-UP Name the batch Enter Name the batch. Start ProtoCOL 3 software Enter Log on to ProtoCOL 3. Total plate count Position the counting Click Position plate frame over the colonies Insert the plate holder, ensuring to be counted.

  • Page 14: Inhibition Zone Batch Set-Up

    INHIBITION ZONE BATCH SET-UP Start ProtoCOL 3 software Choose an application Log on to ProtoCOL 3. Enter Select Select Inhibition Zone. Position plate Name the batch Insert the plate holder, ensuring the plate holder used gives the best contrast between the Enter zone and background.