Expedeon Gelfree 8100 User Manual
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Expedeon Gelfree 8100 User Manual

Fractionation system
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GELFREE
Molecular weight fractionation with liquid phase recovery
 
®
8100 Fractionation System
User
Manual

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Summary of Contents for Expedeon Gelfree 8100

  • Page 1 ® GELFREE 8100 Fractionation System Molecular weight fractionation with liquid phase recovery   User Manual...

  • Page 2: Table Of Contents

    Installation and Power Up  10  Loading the Cartridge & Electrode Placement  10  Operating the Software  12  Running the Cartridge  20  Maintenance and Technical Service  25  User Notifications  26  Bibliography  27  Limited Use License  28    © 2013 Expedeon, Inc. All Rights Reserved.  ...

  • Page 3: General Information

    The proprietary GELFREE design allows for concurrent fractionation of up to 8 samples. The system is comprised of the GELFREE 8100 Fractionation Station, a bench-top instrument, and single-use GELFREE 8100 Cartridge Kits. The Station is a programmable power supply that accurately applies electrical potential, stopping at user-specified intervals for fraction collection.

  • Page 4: Specifications

    Storage: 20-25°C, 30-80% relative humidity Operating: 22°C, 30-80% relative humidity GELFREE 8100 Cartridge Buffer Specifications Running Buffer: 500 mL Sample Buffer: 1.9 mL Temperature Limits: Storage: 20-25°C, 30-80% relative humidity Operating: 22°C, 30-80% relative humidity © 2013 Expedeon, Inc. All Rights Reserved.

  • Page 5: Safety Information

    8100 Fractionation System (5 of Safety Information During operation, the GELFREE 8100 Fractionation Station lid must always remain closed to ensure the safety of the operator. This product is safe to use when operated in accordance with this instruction manual. If this unit is used or modified in a manner not specified in this manual, then protection afforded by the unit will be impaired.

  • Page 6: Unpacking And Placement

    (6 of Unpacking and Placement Unpacking and Inspection of Parts Upon receipt of your GELFREE 8100 Fractionation Station, open the box, carefully remove the foam packaging, and remove the instrument and power cord from the box. Remove the tape securing the lid, along with the protective plastic, so that the lid can operate freely.

  • Page 7: Description Of Parts

    ELFREE 8100 Fractionation Station he GELFREE 8100 Fractionation Station is an independently- controllable, eight channel electrophoretic power supply designed pecifically for use with GELFREE 8100 Cartridge Kits. It features a touch screen display, power supply capable of delivering constant oltage (20-300 V) fo...
  • Page 8 GELFREE 8100 Fractionation System (8 of Description of Parts (cont.) Electrode Array Cartridge  Positioning Key  Figure 3. GELFREE 8100 Fractionation Station. © 2013 Expedeon, Inc. All Rights Reserved.

  • Page 9: Gelfree 8100 Cartridge Kit

    GELFREE 8100 Fractionation System (9 of Description of Parts (cont.) ELFREE 8100 Cartridge Kit he GELFREE 8100 Cartridge is a patented, single use cartridge esigned for insertion into the GELFREE 8100 Fractionation Station. The cartridge is supplied with storage buffer and specially designed,...

  • Page 10: Operating Procedures

    Do not force the electrodes into position – this can permanently damage the mechanism. Very little resistance should be m et when raising and lowering the electrodes into position. © 2013 Expedeon, Inc. All Rights Reserved.
  • Page 11 GELFREE 8100 Fractionation System (11 of Operating Procedures (cont.) Figure 5. GELFREE 8100 Fractionation Station with cartridg and retracted electrodes. Follow arrows to lower electrodes. Figure 6. GELFREE 8100 Fractionation Station with cartridge and lowered electrodes. Follow arrows to lift electrodes.

  • Page 12: Operating The Software

    GELFREE 8100 Fractionation System (12 of Operating Procedures (cont.) Operating the Software Introduction The GELFREE 8100 Fractionation Station is controlled by an onboard computer with a touch screen interface. The primary operating modes are the following: Method Development/Editing Method Execution...
  • Page 13 9           85 V                 05:00 10         85 V                 05:00 Prev Next Figure 8. The GELFREE 8100 Fractionation Station Methods Screen. Touch the ‘Retrieve’ button. This will bring up a dialogue screen (Figure 9) requesting a method number. Begin by pressing the “C” button on the number pad to clea the existing input.
  • Page 14 Retrieve 2           0 V                   00:00 3           0 V                   00:00 Save 4           0 V                   00:00 5           0 V                   00:00 6           0 V  00:00 Apply 7            0 V                   00:00 8             0 V                   00:00 Done 9            0 V                   00:00 10            0 V                   00:00 Prev Next Figure 10. The GELFREE 8100 Fractionation Station. Defin e New Method. © 2013 Expedeon, Inc. All Rights Reserved.
  • Page 15 1           85 V                 05:00 Step 1 Retrieve 2           85 V                 11:00 Voltage (V) 3           85 V                 01:00 Save 4           85 V                 01:00 Time (min) 5           85 V                 02:00 Apply 6           85 V                 02:00 7           85 V                 02:00 Prev Next 8           85 V                 02:00 Done 9           85 V                 05:00 Cancel 10         85 V                 05:00 Prev Next Figure 11. The GELFREE 8100 Fractionation Station. Develop/Edit New Method. © 2013 Expedeon, Inc. All Rights Reserved.
  • Page 16 GELFREE 8100 Fractionation Station incorporate a first step to allow the user to add an additional 2 mL of Running Buffer to the Cathode Buffer Reservoir (bringing the total volume to 8 mL) and to thoroughly rinse the Sample Loading Chamber.
  • Page 17 Channel 6 Channel 7 Channel 8 Done Figure 12. The GELFREE 8100 Fractionation Station Channel Screen. Press the polarity button on the Main Screen to set the polarity of the field. For all experiments using SDS, the polarity should be ‘Negative.’...
  • Page 18 6            85  V    14.8  mA 7            85  V    14.9  mA 8            85  V    14.8  mA Selected Method:  2        Step: 1 of 12 (85 V, 5 min) Lid State:  Figure 13. The GELFREE 8100 Fractionation Station. Abort Experiment. Monitoring a Method: Channel Status: The circles shown in the box on the left side of the Main Screen indi cate the statu s of each channel.
  • Page 19 (will not operate). Software Release Information: Touch the PDI icon found in the top left of the screen to view the current software version running the GELFREE 8100 Fractionation Station. © 2013 Expedeon, Inc. All Rights Reserved.

  • Page 20: Running The Cartridge

    + 8 µL 1M DTT (optional, not provided in the kit) + x µL H e. = 150 µL Note: If the sample volume is less than 112 µL, add 18MΩ O to bring the final volume to 150 µL. © 2013 Expedeon, Inc. All Rights Reserved.
  • Page 21 Buffer Reservoirs, simply flush the membrane with Running Buffer using a pipette. Note: Overfilling the reservoir at this point may result in irregular sample migration and separation. © 2013 Expedeon, Inc. All Rights Reserved.
  • Page 22 Correct voltage and current displayed for all channels (as set by user). Buffer in all Cathode and Anode Buffer Reservoirs. All electrod es contacting buffer. Buffer in all Sample Collection Chambers. No bubbles around the membranes restricting current flow. © 2013 Expedeon, Inc. All Rights Reserved.
  • Page 23 (4-5), placing fraction 2 into colu n 2 of the 96-well pl ate, fraction 3 into column 3, a so on. © 2013 Expedeon, Inc. All Rights Reserved.
  • Page 24 Once all of the Method’s steps have been completed, the display screen will indicate that the experiment has ended. Collect the final fraction and enjoy the benefits of reproducible, high yield, liquid-phase fractionation in y our protein research. © 2013 Expedeon, Inc. All Rights Reserved.

  • Page 25: Maintenance And Technical Service

    Maintenance and Technical Se i rv ce Cleaning Specifications Gener al maintenance for the GELFREE 8100 Fractionation System includ es wiping the unit with a soft dry or slightly dampened cloth. Do no t handle or clean electrodes. Do not use solvents or deterg ents.

  • Page 26: User Notifications

    This warranty limits Expedeon's liability only to the cost of the product. Expedeon makes every effort to ensure the accuracy of its t realizes that the occasional typographical or other publications, bu error is inevitable. Therefore, Expedeon makes no warranty...

  • Page 27: Bibliography

    GELFREE 8100 Fractionation System (27 of Bibliography Tran, J. C., Doucette, A. A. (2008). Gel-Eluted Liquid Fraction Entrapment Electrophore sis: An Electrophoretic Method for Broad Molecular Weight Range Proteome Separation. Analytical Chemistry, 80, 1568-1573. © 2013 Expedeon, Inc. All Rights Reserved.

  • Page 28: Limited Use License

    Product(s) from Expedeon or an authorized distributor or reseller of Expedeon products (in each case a “Buyer”) has a limited no transferable license to use the Product(s) for research purpos only and excluding any Non-Licensed Use. Buyer acknowledges...
  • Page 29 Product(s) at their own cost and will receive a full refund (excluding shipping, tax, and handling fees). For information on purchasing a license to the Product(s) for purposes other than research use, contact us info@expedeon.com © 2013 Expedeon, Inc. All Rights Reserved.
  • Page 30 8100 Fractionation System (30 of Corporate Headquarters www.Expedeon.com...

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