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Summary of Contents for WPI STX2-PLUS
- Page 1 WORLD PRECISION INSTRUMENTS INSTRUCTION MANUAL STX2-PLUS for use with EVOM3 Epithelial Voltohmmeter Serial No._____________________ www.wpiinc.com 082620...
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Page 3: Table Of Contents
Ease of Use ........................... 3 Adjusting the Length of the Electrodes ................4 MAINTENANCE ..........................5 Cleaning/Maintaining the STX2-PLUS Electrodes.............. 5 Disinfecting the STX2-PLUS Electrodes ................5 Sterilizing the STX2-PLUS Electrode ..................6 Rechloriding the Electrodes ....................6 Storing the Electrodes ......................6 Testing the STX2-PLUS Electrodes .................. - Page 4 World Precision Instruments...
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Page 5: About This Manual
Fig. 1—The STX2-PLUS electrode is designed for use with the EVOM3 meter. INTRODUCTION The STX2-PLUS electrode can be used with the EVOM3 and is designed to facilitate measurements of membrane voltage and resistance (TEER) of cultured epithelia directly in 12- and 24-well tissue culture plates. The electrode incorporates a adjustable pair of probes, 5 mm tall, 4 mm wide and 0.7 mm in thickness. -
Page 6: Unpacking
Concealed damage should be reported at once to the carrier and an inspection requested. Please read the section entitled “Claims and Returns” on page 11 of this manual. Please contact WPI Customer Service if any parts are missing at 941.371.1003 or customerservice@wpiinc.com. -
Page 7: Operating Instructions
Ease of Use The STX2-PLUS is designed for use with Corning 347X 24-well plates or Millipore hanging inserts. The apical volume is 250 µL, and the basal volume is 750 to 1000 µL. -
Page 8: Adjusting The Length Of The Electrodes
Align the electrodes with the well inserts and position the STX2-PLUS over the well so that it rests securely in an upright position. (Images shown with a Corning 3470 24-well insert and plate. For greater stability, rest the cable on the tabletop. For other plates and inserts, center the electrodes and the weight holds the electrode in place when making measurement readings. -
Page 9: Maintenance
Ortho-phthaladehyde (Cidex OPA or Rapicide OPA), 30/70 ethanol or 30/70 isopropyl alcohol are also acceptable. The STX2-PLUS electrodes require a 10-15 minute soak in a solution of 5% sodium hypochlorite (undiluted household bleach) to revitalize the electrodes. -
Page 10: Sterilizing The Stx2-Plus Electrode
CAUTION: Ammonia is NOT recommended, because silver chloride dissolves in ammonia. Sterilizing the STX2-PLUS Electrode The STX2-PLUS electrodes are non-sterile as supplied. Acceptable low temperature steril- ization methods for the electrodes include gamma irradiation and ethylene oxide gas (ETO). CAUTION: Do NOT autoclave the electrode. -
Page 11: Testing The Stx2-Plus Electrodes
Regular maintenance of the electrode is strongly recommended for longevity. ACCESSORIES 99675 EVOM2 Adaptor Cable 7363-4 Enzol Enzymatic Detergent Fig. 10—Plug the STX2-PLUS into the 99675 cable to use the electrode with an EVOM2. The 99675 cable is sold separately. World Precision Instruments... -
Page 12: Troubleshooting
Turn off or move any cellular phones farther away from the experimental setup. NOTE: If you have a problem/issue with that falls outside the definitions of this troubleshooting section, contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.com. * Defining Term: •... -
Page 13: Appendix A: Kcl Testing Mixtures
STX2-PLUS APPENDIX A: KCL TESTING MIXTURES When making a variety concentrations of KCl solution, use the following table as a guide. Start by making a solution of 160 mM concentration. To make 100 mL of 160 mM KCl: Add 1.192 gm KCl to 100 mL of distilled water. (1M KCl = 74.54 gm/M in 1 L DI water) - Page 14 Membrane Millicell Pore Size (μm) Qty/Pkg MCHT24H48 MCRP24H48 MCSP24H48 MCMP24H48 MCEP24H49 World Precision Instruments...
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Page 15: Warranty
WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product. - Page 16 175 Sarasota Center Blvd., Sarasota FL 34240-9258 Tel: 941-371-1003 • Fax: 941-377-5428 • E-mail: sales@wpiinc.com 1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ Tel: 44 (0)1462 424700 • Fax: 44 (0)1462 424701 • E-mail: wpiuk@wpi-europe.com Germany Pfingstweide 16, D-61169 Friedberg (Hessen), Germany Tel: +49 (0)6031 67708-0 •...
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