8.3 Acquiring a Live Image Sequence ................14 9. CONTROLLING THE CAMERA (XCAP) ................ 15 9.1 EM Gain ........................15 9.2 Triggering Modes and Pixel Clock Rate ..............16 9.3 Region of Interest and Binning ................... 16 9.4 Exposure Time and Frame Period ................17 Kestrel/USER-MANUAL/REV1.1/08-20...
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9.5 Maximum Frame Rates ..................... 18 9.6 Thermoelectric Cooler (TEC) ..................18 9.7 Information Tab ......................19 10. XCAP CONTROL FEATURES ..................20 10.1 Saving Preset Settings .................... 20 10.2 Contrast Modification (XCAP Std. Only) ..............21 Kestrel/USER-MANUAL/REV1.1/08-20...
1. INTRODUCTION This document provides detailed instructions for the operation of the Kestrel, Scientific EMCCD camera. Raptor Photonics Limited reserves the right to change this document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.
The Camera Link digital interface provides the most stable platform for data transfer and the camera will work on any Camera Link standard frame grabber. 3.2 Datasheet For the full specification of the Kestrel, the datasheet for the camera can be downloaded from the Raptor Photonics website: https://www.raptorphotonics.com/products/kestrel-emccd/...
4. DESIGN OVERVIEW 4.1 Mechanical Model Units shown in mm and [inches] PDF of mechanical model available from our website: https://www.raptorphotonics.com/products/kestrel-emccd/ Kestrel/USER-MANUAL/REV1.1/08-20...
The camera has a standard C-mount that should easily screw onto any microscope port. 4.5 Mounting to a Tripod or Optical Table The camera has a ¼-20 BSW (Whitworth), threaded hole to mount to a tripod or an optical table. Kestrel/USER-MANUAL/REV1.1/08-20...
5. SOFTWARE COMPATIBILITY This section outlines the options relating to software that are available for the Kestrel camera. 5.1 XCAP Compatibility Raptor works closely with EPIX who integrate all the Raptor camera models into their XCAP Imaging Software package. XCAP is the core plug and play software package that is offered with Raptor cameras.
1. Connect the T257P chiller to the Kestrel using the tubing provided. You will hear a click which indicates a solid connection. The polarity of the tubing connections does not matter.
Raptor. Shorter draining tubes will be supplied with the demo kit. Two draining tubes (with the appropriate mating connectors) need to be connected to both connecters on each of the camera, chiller and tubing to drain each individually. Kestrel/USER-MANUAL/REV1.1/08-20...
The amount of bandwidth required depends on the camera in- hand. The Kestrel uses a Base Camera Link interface which can be handled with a x1 PCIe bus and PIXCI EB1, providing roughly 200MB/sec maximum bandwidth. Contact EPIX Inc.
A PIXCI Open/Close pop-up box will open as shown in Figure 2. Figure 2: PIXCI Open/Close. Click on “Camera & Format” that is highlighted in Figure 2 and a “PIXCI Open Camera & Format” box will appear, as shown in Figure 3. Kestrel/USER-MANUAL/REV1.1/08-20...
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Using the dropdown menu highlighted, search for “Raptor Photonics Kestrel”. Alternatively, you can use the search button and typing Kestrel will bring up the correct configuration. Selecting “Open w. Default Video Setup” will open the control panel with all control parameters set to the default states.
Clicking the “Live” button will grab a live image sequence which you will now see in the imaging window. For the Kestrel, you will have to enable contrast modification after going live. For instruction on how to do this on XCAP, please refer to section 10.2.
9. CONTROLLING THE CAMERA (XCAP) The sections below will give information on using each control of the Kestrel, giving a description on how to use each control parameter and their effect on the camera’s performance. The software used to illustrate the camera controls is XCAP.
ITR mode is closer to exposure time + readout time. Btn. Triggered: This mode enables the user to capture a single exposure. The “Single Exposure” button in the GUI will become active and clicking it will trigger an exposure time and capture. Figure 7: Trigger Tab. Kestrel/USER-MANUAL/REV1.1/08-20...
If the exposure time set exceeds the maximum possible under the given frame period, then exposure will dominate, and the frame period will be increased. The frame period and exposure time controls on XCAP are shown in Figure 9. Figure 9: Exposure Time & Frame Period Kestrel/USER-MANUAL/REV1.1/08-20...
The actual TEC set point of the camera should be within ±2 ͦ C of the TEC set point set by the user. The sensor temperature will then have a stability of ±0.5 ͦ C around the actual TEC set point. The TEC controls in XCAP are shown in Figure 10. Figure 10: TEC Control Tab. Kestrel/USER-MANUAL/REV1.1/08-20...
The “Info” tab displays the manufacturers data of the camera, such as the firmware version, serial number etc. The sensor and PCB temperature can also be read from this tab. The “Info” tab is shown in Figure 11. Figure 11: Info Tab. Kestrel/USER-MANUAL/REV1.1/08-20...
The overall settings file can then be saved and loaded in this tab also. Three preset states is the maximum number that can be saved in a settings file. Figure 12: Preset Settings. Kestrel/USER-MANUAL/REV1.1/08-20...
The contrast can be adjusted using the low and high end percentile point controls. The default settings are usually adequate for most applications. NOTE: This control feature is only applicable with XCAP Std. Figure 14: Contrast Modification. Kestrel/USER-MANUAL/REV1.1/08-20...
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CORPORATE HEADQUARTERS Raptor Photonics LTD Willowbank Business Park Larne, Co Antrim BT40 2SF Northern Ireland PH: +44 2828 270141 www.raptorphotonics.com Kestrel/USER-MANUAL/REV1.1/08-20...
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