LightOx PhotoReact 365 User Manual

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User Manual

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PhotoReact 365

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lightox.co.uk

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Summary of Contents for LightOx PhotoReact 365

Page 2: Table Of Contents
No part of this publication may be reproduced, transmitted, transcribed, stored in a retrieval system or translated into any language in any form by any means without the prior written consent of LightOx Limited. Every effort has been made to ensure information in this manual is accurate, however, information in this manual is subject to change without notice and does not represent a commitment on the part of the authors.
Page 3: Introduction
Key Features • The PhotoReact 365 is a bench top instrument for the acceleration of photochemical and photobiological reactions • Instrument suitable for use with a standard size culture multi-well plate (85.5 mm x 128 mm) or single 95 mm round dish...
Page 4: Safety
2. Safety Prior to use, the operator should thoroughly read the instructions for use. Using this device without reading and understanding the instructions for use may result in operator injury or damage to the equipment. The device contains powerful LEDs that produce bright UV light.
Page 5: System Overview
3. System Overview PhotoReact 365 is a device that can deliver a specific amount of light to a sample plate in a controlled environment. Controlling the power (light intensity) and irradiation time (seconds) enables specific energy across the whole sample plate.
Page 6: Warnings
Ensure the ventilation slots on the side of the unit are unobstructed. Not for domestic use. In accordance with EN 55011: 2009 +A1:2010 the PhotoReact 365 is a Class A product. In a domestic enviroment this product may cause radio interference in which case the user may be required to take adequate measures.
Page 7: System Components
4. System Components The PhotoReact 365 is comprised of the following: Base Unit Removable, locking lid Control Unit USB cable connecting Control Unit to Base Unit Power Supply User Manual...
Page 8: Choosing A Location
4.2. Choosing a Location PhotoReact 365 Place the on a flat surface on its neoprene feet with at least 15 cm of space between the perimeter of the instrument and the nearest object. PhotoReact 365 is designed to work at ambient room temperature.
Page 9: Connecting The Power Cord And Usb Cable
4.3. Connecting the Power Cord and USB cable Insert the one end of the USB cable from the USB port on the right side of the Control Unit. Insert the other end of the USB cable into the USB port on the Base Unit.
Page 10: Setup
5.1. Setup Operation Once the power cord and the USB cables are connected PhotoReact 365 Base Unit and Control Unit are ready for operation. The display start-up screen is shown below. The touch screen buttons are New experiment, Rerun experiment and the system Settings button.
Page 11: Setting The Date/Time
5.2. Setting the date/time Operation Copy logs to flash drive Clear all logs Set date and time Settings (Administrator password required) About Back Set date and time Month Year Hour Minute Second Back Save Select the Set date and time button and use the up and down toggle buttons to set the date (day, month and year) and time (hour, minute and second) in two digit format.
Page 12: Performing An Irradiation Experiment 12>16
5.3. Performing an irradiation experiment Placing Sample Plate PhotoReact 365 Remove the lid by turning the lid counter-clockwise fully until it stops and lift the lid upwards with two hands. Take the lid off, place the multi-well or tissue culture plate on top of the glass surface in preferred orientation.
Page 13 Replace the lid ensuring the lid locking pins align with the round holes on the Base Unit. Turn the lid clockwise fully to lock the lid into place. Note: The PhotoReact 365 will not function unless the lid is fully engaged in the lock position. Replace lid...
Page 14 5.3. Performing an irradiation experiment Running Experiment. Step 1. Next, on the display start-up screen of the Control Unit, select either New experiment or Rerun experiment. After selecting New experiment enter the experiment name using the touch keyboard on the next screen. Select Save to bring up the experiment settings screen shown below.
Page 15 5.3. Performing an irradiation experiment Running Experiment. Step 2. The following settings can be customised. Trial Duration: Duration: 5 minutes 30 seconds Hold (Time; MM:SS) up to 30:00 Irradiance: Irradiance: 6.5 mW/cm2 from 0 mW/cm2 to the maximum Energy: 2145 mJ/cm2 (approx.
Page 16 5.3. Performing an irradiation experiment Running Experiment. Step 3. Trial - 01/04/2020 10:40:03 Time remaining: 4:29 Duration: 5:30 Temp: Intensity: 65 mW/cm 100% Energy density: 2145 mJ/cm Abort If operating correctly the fans will turn on and the experiment run screen will be shown as above. Warning: Risk of UV LIGHT Exposure may result in injury to eyes or skin.
Page 17: Re-Running An Experiment
5.4. Re-running an experiment Operation Warning: Risk of UV LIGHT To re-run an experiment using the same experimental parameters, select Rerun experiment on the display start-up screen. Select the experiment name from the list. Use the Next button to scroll through additional pages of experimental names.
Page 18: Downloading Experimental Data 18>20
5.5. Downloading experimental data Copy logs to flash drive. Step 1. To download data files to a flash drive, press the systems setting button on the start up screen. Copy logs to flash drive Clear all logs Set date and time Settings (Administrator password required) About Back...
Page 19 5.5. Downloading experimental data Copy logs to flash drive. Step 2. Insert a USB drive into the Data Port on the back of the Control Unit, next to the power connector. Once inserted, the Control Unit will automatically start downloading log files to the USB Drive: Export log files Exporting file LOG00000.csv...
Page 20 5.5. Downloading experimental data Copy logs to flash drive. Step 3. Once all files are downloaded an ‘Export complete’ prompt will display: Export log files Export complete 43 log files copied Done It’s now safe to remove the USB drive, and press the Done button to be taken back to the settings screen.
Page 21: Clearing Log Files 21>22
5.5.1 Clearing log files Clear all logs At any time, it’s possible to clear all log files from the controller. Typically you’ll do this after downloading the files to the USB drive. To clear all log files select the Clear all Logs button on the settings screen: Copy logs to flash drive Clear all logs Set date and time...
Page 22: Termination, Turning The Unit "Off
5.5.1 Clearing log files Clear all logs Continued Logs will be deleted and once cleared a message ‘Logs cleared’ will display. Press the Back button to return to the settings screen. Clear all logs Logs cleared Back 5.6. Termination, Turning the Unit “Off” Unplugging the unit or switching off the unit while it is running will cause no harm to the system, nor will it cause an unsafe condition.
Page 23: System Information
About on the system information screen. 7. Proper Care and Handling Remove the PhotoReact 365 and accessories from packaging upon arrival. Visually inspect all components supplied and immediately notify the supplier of any defects detected.
Page 24: Maintenance
Maintenance only to be performed by trained approved operator. 9. Troubleshooting Q: My screen does not display LightOx logo on startup. A: Unplug the power supply (or turn power off at supply), wait at least 10 seconds and reinsert (or switch on) the power supply.
Page 25: Technical Data
10. Technical Data Base Unit with Lid Diameter: 21.0 cm Height: 18.5 cm LED wavelength: 365 nm Control Unit Width: 21.0 cm Height: 5.4 cm Depth: 9.0 cm Weight: Approximately 6.4 kg (base unit with lid) and 1.2 kg (controller) Power supply input: 100-240V, 50/60 Hz, 0.8A (AC) and fluctuations up to 10% of the nominal voltage Power supply output: 12V (DC), 3A...