Table of Contents
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TG
Manual ___________________________________________________________ 1
RADIENT
Manual
Software Version 4.20
May 2003
Order-No.
050-800 TGradient 48
050-801 TGradient 96
Biometra
biomedizinische Analytik GmbH
Rudolf-Wissell-Str. 30, D-37079 Goettingen
Tel.: 0551/50 686-0; Fax: 0551/50 686-66
email:
info@biometra.co.uk
http://www.biometra.com
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Table of Contents
Troubleshooting
Summary of Contents for Biometra TGRADIENT 48
- Page 1 Manual ___________________________________________________________ 1 RADIENT Manual Software Version 4.20 May 2003 Order-No. 050-800 TGradient 48 050-801 TGradient 96 Biometra biomedizinische Analytik GmbH Rudolf-Wissell-Str. 30, D-37079 Goettingen Tel.: 0551/50 686-0; Fax: 0551/50 686-66 email: info@biometra.co.uk http://www.biometra.com...
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Page 2: Introduction
RADIENT 1 Introduction The TG is the new high end thermocycler in the Biometra product line. It was our goal RADIENT to develop an instrument that provides fast optimization of reaction parameters as well as a high performance for daily routine analysis. Both could be realized by a completely new block architecture and the latest Peltier technology. -
Page 3: Before You Start
Manual ___________________________________________________________ 3 RADIENT 2 Before you start Safety warnings Before connecting the unit to the power source please ensure that the voltage selector at the back side of the instrument is set to the required voltage. Danger of electric shock! Unplug the power cable before you open the Thermocycler. -
Page 4: First Steps With The Tg Radient
Manual ___________________________________________________________ 4 RADIENT 3 First steps with the TG RADIENT The TG Thermocycler front View RADIENT Wheel for the height adjustment Knob for release of the heated lid. of the lid Keypad Graphical display Power switch... -
Page 5: The Tg Radient Rear View
Manual ___________________________________________________________ 5 RADIENT The TG rear view RADIENT Parallel printer port RS-232 port Fuse holder for 2 fuses, Power cord for specifications of fuses Type label including: plug see type label on the serial number instrument´s backside specifications of fuses... -
Page 6: The Tgradient Control Panel
Manual ___________________________________________________________ 6 RADIENT The TGradient control panel Graphical display Power Easy to clean keyboard Power switch: backlit = power on Cursor keys: For free navigation in the Function keys: programming spreadsheet. the corresponding functions are Use to scroll through lists. specified in the screen. -
Page 7: Initial Self Test
Manual ___________________________________________________________ 7 RADIENT Initial self test After switching on the TG the serial number of the instrument and the software version RADIENT is displayed. TGradient Serial No 1234567 Vers. 4.00 op The TG checks all programs in all directories (so called RAM check). RADIENT The TGradient display The TG... -
Page 8: Releasing Wheel In Case Of Blocked Lid
Manual ___________________________________________________________ 8 RADIENT Releasing wheel in case of blocked lid Note: When the lid is in the extreme up or down position, it may happen that the wheel is uncoupled. In this situation the clutch mechanism is active in both directions (clicking noise in either direction). -
Page 9: Creating A Program
Manual ___________________________________________________________ 9 RADIENT 4 Creating a Program Select a directory In the TG programs can be stored both in the main directory and in individual RADIENT subdirectories. For easy identification of the selected storage place, the subdirectories can be named (see section 4.3). -
Page 10: Enter Name For Subdirectory
Manual __________________________________________________________ 10 RADIENT Enter name for subdirectory The process for naming a subdirectory and a program is the same. For information about naming programs see section 4.4. Press [D enter] to enter the subdirectory directory: program no. Name: edit A list C quit D enter... -
Page 11: Enter The Lid Temperature
Manual __________________________________________________________ 11 RADIENT Confirm each letter with [D enter]. Directory: program no. Name: Ù ABCDEFGHIJKLMNOPQRST UVWXYZ-()#°C/,áñ&+.%! A name B blank C name OK D enter The selected letter will appear in the display and the cursor will move one step to the right. Select the next letter and confirm with [D enter]. -
Page 12: Enter Temperature And Time Settings
Manual __________________________________________________________ 12 RADIENT Directory: program no. Name: TEST 1 lid temp: 105 °C preheating: on edit A on/off B files C pgm OK D enter Note: The default setting, which is recommended for most applications, is preheating: on. After all pre-settings have been made, press [D enter] to open the programming spreadsheet. Enter temperature and time settings The programming spreadsheet: ß... -
Page 13: Set Temperature Gradient
B insert/delete C pgm OK D enter Enter a time for step one. Note: There is a convention on how time settings are entered in all BIOMETRA cyclers: hours minutes seconds If you enter a number without “point” this value will be interpreted as seconds (“300” => 5 minutes). -
Page 14: Set Cycle Number
Manual __________________________________________________________ 14 RADIENT ß opt à Temp[°C] time gradient[°C] T1 – 6: 50.0 50.2 50.9 52.0 53.2 54.4 °C T7 –12: 55.6 56.8 58.0 59.1 59.8 60.0 °C 55.0 0h 0m30s 10°C Temperature difference left side / right side In the first highlighted lane the temperatures from row 1 to 6 are displayed. -
Page 15: Save Program
Manual __________________________________________________________ 15 RADIENT Note: The minimum temperature you can set is –3°C. 4.12 Save program Save program with [C pgm OK]. The program data are stored in the permanent memory. Directory: program no. Name: TEST 1 number of steps: 5 runtime: 1h10m23s edit... -
Page 16: View/Edit Program Data
Manual __________________________________________________________ 16 RADIENT 5 View/edit program data You can check any existing program by scrolling through the editing mode with cursor keys ïðñò. Select the desired program as described in section 4.2. Note: During operation of the cycler the active program can be viewed but not modified. If you want to change settings of the active program for further experiments you have to save a copy of this program to another memory. -
Page 17: Copy Program
Manual __________________________________________________________ 17 RADIENT insert / delete step: 2: 95.0 0h 0m30s 3: 55.0 0h 0m30s 4: 72.0 0h 0m30s A insert B delete C quit D enter Press [A insert] to insert one step at the selected position. Note: Additional steps will be inserted before the selected step. The following steps will be shifted by one position. -
Page 18: Delete Program
Manual __________________________________________________________ 18 RADIENT ½ copy to: -3. subdirect.: ½ -4. subdirect.: ½ Main direct.--- -5. Subdirect.: ½ -6. subdirect.: ½ -7. subdirect.: C quit D enter Confirm your selection with [D enter]. copy from directory 3 program 0 directory 6 program A list C quit D enter... - Page 19 Manual __________________________________________________________ 19 RADIENT Directory: program no. Name: TEST 1 lid temp: °C preheating: on delete program 0? enter = yes A copy B delete pgm C quit D enter Confirm your selection with [D enter]. Note: Once you have deleted a program it cannot be restored!
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Page 20: Further Programming Options
Manual __________________________________________________________ 20 RADIENT 6 Further programming options ß opt à Temp[°C] time gradient[°C] 1: 95.0 0h 5m 0s 2: 95.0 0h 0m30s 3: 55.0 0h 0m30s 4: 72.0 0h 0m30s B header C pgm OK D enter Note: To program special parameters you can scroll the display to the right. Move the cursor to the right side of the display that is labeled with opt à. -
Page 21: Program Touch Down
Manual __________________________________________________________ 21 RADIENT Note: The time increment will have an impact on the total runtime depending on the numbers of cycles and the size of the increment. A program with many cycles and large time increments will take a significantly longer time than a standard protocol. Program touch down For some applications it is useful to start with a higher annealing temperature and to decrease the temperature from cycle to cycle. -
Page 22: Run Program
Manual __________________________________________________________ 22 RADIENT 7 Run Program Select and start program Main display: Prog step temp[°C] 25.2°C time 25.4°C B start/stop C programs To select a program press [B start/stop] ½ start -3. subdirect.: ½ -4. subdirect.: ½ Main direct.--- -5. -
Page 23: Display During Operation
Manual __________________________________________________________ 23 RADIENT 0 test 1 1 empty 2 empty 3 empty 4 empty A list B forward C quit D enter Confirm highlighted program with [D enter]. The number and name of both subdirectory and the selected program will be displayed. Directory: program no. -
Page 24: View Remaining Run Time
Manual __________________________________________________________ 24 RADIENT The elapsed time versus programmed hold time is displayed Prog 0 TEST 1 dir 3 Step cycle 1 of 30 49.9 55.0 60.0 à 50.0 55.0 60.0 temp[°C] à0h 5m 0s time 0h 0m23s 104.9°C A info B start/stop C programs As soon as the block enters a gradient step the three temperatures of the left, middle and right... -
Page 25: Pause / Stop Program
Manual __________________________________________________________ 25 RADIENT Temperatures of current gradient T 1- 6: 49.4 49.6 50.3 51.4 52.6 53.8 °C T 7-12: 55.0 56.2 57.3 58.4 59.1 59.4 °C remaining time: 2h56m A gradient C quit Note: The temperatures can be displayed online during each gradient step (see section 7.4). As the program moves to the next step the temperature data of the last gradient are stored in the temporary memory. -
Page 26: Special Functions
Manual __________________________________________________________ 26 RADIENT 8 Special functions Main screen Prog step temp[°C] 25.2°C time 25.4°C B start/stop C programs To change special features of the TG press [D +]. RADIENT 1 print 2 signal 3 language 4 standard mode 5 test mode A back B forward C quit... -
Page 27: Select Language
Manual __________________________________________________________ 27 RADIENT Select language The display of the TG can be switched from English to German. Select language with RADIENT cursor keys ò ñ. As you toggle between the two settings the language will change. language German English C quit D enter To confirm your selection press [D enter]... -
Page 28: Maintenance
Manual __________________________________________________________ 28 RADIENT 9 Maintenance The TG was built to operate for a long time without the need for periodical RADIENT maintenance. The housing may be cleaned from time to time with a smooth cotton cloth. Do not use strong detergents or organic solvents for cleaning. 10 Exchange of modules For exchange of modules you need two hexagonal keys (number 3 and number 5). -
Page 29: Trouble Shooting
Manual __________________________________________________________ 29 RADIENT 11 Trouble shooting 11.1 Slow heating and cooling The T1 T is equipped with a strong ventilator for the cooling of the heat sink. The HERMOCYCLER inlet of this fan is located at the bottom side of the instrument. Be sure that the inlet is not clogged by dust or other material (e.g. -
Page 30: Trouble Shooting
Manual __________________________________________________________ 30 RADIENT 12 Trouble shooting 12.1 Slow heating and cooling The TG is equipped with a strong ventilator for the cooling of the heat sink. The inlet of RADIENT this fan is located at the bottom side of the instrument. Be sure that the inlet is not clogged by dust or other material (e.g. -
Page 31: Appendix
050-811 RADIENT 384 Module for 384 well microplates (non gradient) 050-812 RADIENT in situ Module for 4 microscopic slides (non gradient) 050-813 RADIENT For information about tubes, strips, microplates and other consumables please contact BIOMETRA or your local BIOMETRA distributor. -
Page 32: Instructions For Return Shipment
RADIENT · Return only defective devices. For technical problems which are not definitively recognizable as device faults please contact the Technical Service Department at Biometra ( Tel.: +49/551/5068640, Fax: ++49/551/5068666, e-mail: info@biometra.co.uk). · Important: Cleanse all parts of the instrument of biologically dangerous, chemical or radioactive contaminants. -
Page 33: Warranty
This warranty covers defects in materials or workmanship for 24 months as described under the following conditions: This warranty is valid for 12 month from date of shipment to the customer from Biometra or an authorized distributor. This warranty will not be extended to a third party without a written agreement of Biometra. -
Page 34: Equipment Decontamination Certificate
NAME ________________________________ POSITION __________________________ (HEAD OF DIV./ DEP./ INSTITUTE / COMPANY) SIGNED ______________________________ DATE ______________________________ PLEASE RETURN THIS FORM TO BIOMETRA GmbH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT. PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING. INSTRUMENTS WITHOUT THIS... -
Page 35: Table Of Contents
Manual ____________________________________________________________I RADIENT Introduction ..........................2 Before you start ........................3 2.1 Safety warnings ........................3 2.2 Warning symbols.........................3 First steps with the TG ....................4 RADIENT 3.1 The TG Thermocycler front View.................4 RADIENT 3.2 The TG rear view ....................5 RADIENT 3.3 The TGradient control panel ....................6 3.4 Initial self test ........................7 3.5 The TGradient display......................7 3.6 Handling of the adjustable heated Lid.................7... - Page 36 Manual ___________________________________________________________ II RADIENT 12 Trouble shooting ........................30 12.1 Slow heating and cooling ....................30 12.2 Restart due to unrecognized power failure..............30 12.3 Adaptation of protocols from other cyclers..............30 13 Appendix ..........................31 13.1 Technical specification TGradient 96 ................31 13.2 Ordering information.....................31 14 Instructions for return shipment ....................32 15 Warranty..........................33 16 Equipment Decontamination Certificate................34...
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