WESCOR AEROSPRAY GRAM 7322 Applications Manual

Slide stainer/cytocentrifuge
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AEROSPRAY
 
    S LIDE  
STAINER/CYTOCENTRIFUGE  
 
 
Model   7 322  
 
 
Applications   M anual  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
57-­‐2002-­‐01A  
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GRAM  

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  • Page 1           ® AEROSPRAY GRAM         S LIDE   STAINER/CYTOCENTRIFUGE       Model   7 322       Applications   M anual                  ...
  • Page 2   w hatsoever,   w ithout   t he   p rior   e xpress   w ritten   p ermission   o f   W escor,   I nc.     Wescor,   A erospray   a nd   C ytopro   a re   t rademarks   o f   W escor,   I nc,   a n   E LITech   G roup   Company.  ...
  • Page 3: Table Of Contents

    Table   o f   C ontents   Section   1   Introduction       1.1   A erospray G ram   S tainer   O verview..........6   Using   t his   M anual..............6   Functional   D escription ............7   Key  ...
  • Page 4 Section   3     Controlling   a nd   C ustomizing   S tainer   F unctions   ( continued)   Creating   U ser   A ccounts ...........34   Managing   U ser   A ccess ............35   User  ...
  • Page 5 Section   5     Preventive   M aintenance   a nd   S afety   ( continued)   5.4   C leaning   t he   S tainer   a nd   C arousels..........57   Cleaning   L iquid   S pills .............57   Cleaning  ...
  • Page 6: A Erospray G Ram   S Tainer   O Verview

    SECTION   1   INTRODUCTION     1.1   A erospray   G ram   S tainer   O verview       Using   t his   M anual   This   m anual   p rovides   i nstructions   t o   i nstall,   o perate,   a nd   m aintain   t he   A erospray   G ram   Stainer/Cytocentrifuge.  ...
  • Page 7: Functional   D Escription

    SECTION   1   INTRODUCTION     1.1   A erospray   G ram   S tainer   O verview     WARNING!   Reagents   u sed   i n   t he   G ram   s tainer   c ontain   m oderately   h azardous   c hemicals   t hat   require  ...
  • Page 8: Key   F Eatures

    SECTION   1   INTRODUCTION       1 .1   A erospray   G ram   S tainer   O verview       Key   F eatures   The   k ey   f eatures   o f   t he   A erospray   G ram   s tainer   a re:   Minimized  ...
  • Page 9 SECTION   1   INTRODUCTION       1 .1   A erospray   G ram   S tainer   O verview       Table   1 :   G eneral   S pecifications     Category   Characteristics  ...
  • Page 10 SECTION   1   INTRODUCTION     1.1   A erospray   G ram   S tainer   O verview       Table   1 :   G eneral   S pecifications   ( continued)     Category  ...
  • Page 11 SECTION   1   INTRODUCTION     A erospray   G ram   S tainer   O verview         Table   3 :   R un   T ime   S equence     NOTE:  ...
  • Page 12 SECTION   1   INTRODUCTION   1.1   A erospray   G ram   S tainer   O verview     Table   5 :   C arousel   a nd   R otor   I nformation     Only  ...
  • Page 13 SECTION   1   INTRODUCTION     1.1   A erospray   G ram   S tainer   O verview   Table   6 :   E xplanation   o f   S ymbols     Alternating   C urrent   ( AC)    ...
  • Page 14 SECTION   1   INTRODUCTION     1.1   A erospray   G ram   S tainer   O verview   Table   6 :   E xplanation   o f   S ymbols   ( continued)    ...
  • Page 15: I Nstrument   D Escription

    SECTION   1   INTRODUCTION     1.2   I nstrument   D escription       Figure   1 :   F ront   a nd   R ight   S ide   P anels      ...
  • Page 16 SECTION   1   INTRODUCTION     1.2   I nstrument   D escription     Figure   2 :   F ront   P anel   a nd   T ouchscreen       1        ...
  • Page 17 SECTION   1   INTRODUCTION     1.2   I nstrument   D escription       Figure   4 :   S tainer   B owl   C omponents       6                   5                   4                     3             2             1    ...
  • Page 18 SECTION   1   INTRODUCTION     1.2   I nstrument   D escription     Table   7 :   P reventive   M aintenance   K it     Component   Name   Description   Manual   P riming   T ool   Primes  ...
  • Page 19: Barcode   R Eader

    SECTION   1   INTRODUCTION     1.2   I nstrument   D escription   Table   7 :   P reventive   M aintenance   K it   ( continued)     Component   Name   Description   Volume  ...
  • Page 20: T Ouchscreen   A Nd   U Ser   I Nterface

    SECTION   1   INTRODUCTION     1.3   T ouchscreen   a nd   U ser   I nterface   Users   c ontrol   a ll   i nstrument   f unctions   f rom   t he   i nteractive   t ouchscreen   d isplay.  ...
  • Page 21 SECTION   1   INTRODUCTION     1.3   T ouchscreen   a nd   U ser   I nterface   Table   8 :   F ront   P anel/Main   S creen   F unction   K eys   ( continued)    ...
  • Page 22 SECTION   1   INTRODUCTION     1.3   T ouchscreen   a nd   U ser   I nterface   Table   9 :   S ystem   S etup   K eys   ( continued)     Button  ...
  • Page 23: Setup   A Nd   P Reparation   F Or   O Peration

    SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.1   I nstrument   S etup     Unpacking   a nd   I nstalling   t he   S tainer   Follow  ...
  • Page 24: Connecting   T He   D Rain   T Ube   A Nd   W Aste   C Ontainer

    SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.1   I nstrument   S etup   Connecting   t he   D rain   T ube   a nd   W aste   C ontainer   1  ...
  • Page 25: Installing   S Tandard   5 00   M L   R Eagent   B Ottles

    SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.1   I nstrument   S etup     Installing   S tandard   5 00   m L   R eagent   B ottles    ...
  • Page 26: Installing   T He   5   L Iter   R Eagent   B Ottle

    SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.1   I nstrument   S etup   Installing   t he   5   L iter   R eagent   B ottle   NOTE:  ...
  • Page 27: P Reparing   T He   S Tainer   F Or   O Peration

    SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.2   P reparing   t he   S tainer   f or   O peration     Priming  ...
  • Page 28 SECTION   2   SETUP   A ND   P REPARATION   F OR   O PERATION     2.2   P reparing   t he   S tainer   f or   O peration   Priming   P rocedures   ( continued)   6  ...
  • Page 29: The   C Lean   C Ycle

    SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.2   P reparing   t he   S tainer   f or   O peration     The  ...
  • Page 30: Reagent   L Evel   M Onitoring

    SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.2   P reparing   t he   S tainer   f or   O peration   Reagent   L evel   M onitoring   Reagent  ...
  • Page 31: Zeroing   T He   R Eagent   L Evel   S Ensors

    SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.2   P reparing   t he   S tainer   f or   O peration     Zeroing  ...
  • Page 32 SECTION   2     SETUP   A ND   P REPARATION   F OR   O PERATION     2.2   P reparing   t he   S tainer   f or   O peration     5  ...
  • Page 33: Controlling   A Nd   C Ustomizing   S Tainer   F Unctions

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu   Many   s oftware   s ettings   c an   b e   c ontrolled   f rom   t he   S ystem   S etup   m enu,   i ncluding:   Creating,  ...
  • Page 34: Adjusting   T He   D Ecolorizer   L Evel

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu   Adjusting   t he   D ecolorizer   L evel:   1  ...
  • Page 35: Managing   U Ser   A Ccess

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu   8   Enter   a   n umeric   p asscode   ( at   l east   4   n umbers)   f or   t he   u ser   a ccount.    ...
  • Page 36: Using   R Eagent   I Nformation   T Racking

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu   Using   R eagent   I nformation   T racking   You  ...
  • Page 37: Modifying   L Evel   D Etect   F Unctions

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu     Modifying   L evel   D etect   F unctions   The  ...
  • Page 38: System   L Og

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu   System   L og   The   i nstrument   r ecords   a ll   l ogin,   l ogout,   s tain   o r   c ytocentrifuge   c ycles,   s etting   c hanges,   maintenance  ...
  • Page 39: Qc/Maintenance   T Racking

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu     QC/Maintenance   T racking   Under   s ystem   d efault   s ettings,   T he   f ollowing   Q C/Maintenance   T racking   o ptions   a re   d isabled:   Enable  ...
  • Page 40: Enable   C Yto   S Lide   T Racking

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu     Enable   C yto   S lide   T racking   Allows  ...
  • Page 41: Restoring   S Oftware   D Efaults

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.1   S ystem   S etup   M enu     Restoring   S oftware   D efaults   1  ...
  • Page 42: R Ecording   S Pecimen   A Nd   R Eagent   I Nformation

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.2   R ecording   S pecimen   a nd   R eagent   I nformation   Scanning   S lides   w ith   t he   B arcode   R eader   From  ...
  • Page 43: Scanning   R Eagent   B Ottles   W Ith   T He   B Arcode   R Eader

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS       3.2   R ecording   S pecimen   a nd   R eagent   I nformation   Scanning  ...
  • Page 44: Manually   E Ntering   S Pecimen   I Nformation

      SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS       3.2   R ecording   S pecimen   a nd   R eagent   I nformation   Scanning  ...
  • Page 45: T He   H Elp   M Enu

    SECTION   3     CONTROLLING   A ND   C USTOMIZING   S TAINER   F UNCTIONS     3.3   T he   H elp   M enu   The   H elp   m enu   i s   a   c omprehensive   o nscreen   h elp   f unction   t hat   p rovides   d etailed   i nformation   o n   the  ...
  • Page 46: Operating   T He   A Erospray   G Ram

    SECTION   4     OPERATING   T HE   A EROSPRAY   G RAM     4.1   O perating   I nstructions     Suggested   S taining   P rotocol   Hub   P attern   T est   ( once   p er   d ay).   •...
  • Page 47: Loading   T He   C Arousel

    SECTION   4     OPERATING   T HE   A EROSPRAY   G RAM     4.1   O perating   I nstructions     Loading   t he   C arousel     CAUTION:     Never  ...
  • Page 48 SECTION   4     OPERATING   T HE   A EROSPRAY   G RAM     4.1   O perating   I nstructions   Loading   t he   C arousel   ( continued)   If   u sing   a   1 2-­‐slide   c arousel,   l oad   s lides   w ith   t he   l abels   t oward   t he   o uter   r im   •...
  • Page 49: Using   B Locking   S Lides

    SECTION   4     OPERATING   T HE   A EROSPRAY   G RAM     4.1   O perating   I nstructions   Using   B locking   S lides   If   t he   c arousel   i s   n ot   f ull,   b lank   s lides   s hould   b e   u sed   a s   b locking   s lides.   B locking   s lides   prevent  ...
  • Page 50: Unloading   T He   C Arousel

    SECTION   4     OPERATING   T HE   A EROSPRAY   G RAM     4.1   O perating   I nstructions     Performing   a   S tain   C ycle   ( continued) 3  ...
  • Page 51: Monitoring   R Eagent   A Nd   W Aste   L Evels

    SECTION   4     OPERATING   T HE   A EROSPRAY   G RAM     4.1   O perating   I nstructions     Monitoring   R eagent   a nd   W aste   L evels   If  ...
  • Page 52: Replacing   A   R Eagent   B Ottle

    SECTION   4     OPERATING   T HE   A EROSPRAY   G RAM     4.1   O perating   I nstructions     NOTE:   Y ou   c an   a ccess   t he   R eagent   I nformation   m enu   b y   p ressing   t he   b ottle   i cons   o n   t he   right  ...
  • Page 53: Preventive   M Aintenance   A Nd   S Afety

      SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.1   P reventive   M aintenance   The   s ystem   p rovides   a   P reventive   M aintenance   L og   f or   t racking   t he   m ost   r ecent   maintenance  ...
  • Page 54: Monthly   M Aintenance

    SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.1   P reventive   M aintenance     Monthly   M aintenance   1   Disassemble   a nd   m anually   c lean   a ll   n ozzles.   R efer   t o   N ozzle   D isassembly   and  ...
  • Page 55: S Toring   T He   I Nstrument

    SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.2   S toring   t he   I nstrument     If   t he   i nstrument   i s   i nactive   f or   m ore   t han   o ne   w eek,   y ou   m ay   w ant   t o   p erform   t he   l ong-­‐term   storage  ...
  • Page 56: R Eplacing   F Uses

    SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.3   R eplacing   F uses     WARNING!   To   p revent   t he   r isk   o f   f ire,   t he   m ain   f uses   s hould   o nly   b e   r eplaced   w ith   f uses   o f   t he   s ame   t ype   and  ...
  • Page 57: C Leaning   T He   S Tainer   A Nd   C Arousels

    SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.4   C leaning   t he   S tainer   a nd   C arousels   WARNING!   All   c leaning   p rocedures   s hould   b e   p erformed   i n   a   w ell-­‐ventilated   r oom   b y   a uthorized   t rained   personnel  ...
  • Page 58: D Econtaminating   T He   S Tainer   A Nd   C Arousels

    SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.5   D econtaminating   t he   S tainer   a nd   C arousels     All   p arts   o f   t he   i nstrument   t hat   c ome   i nto   c ontact   w ith   b iological   s pecimens,   p atient   s pecimens,   positive  ...
  • Page 59: Decontaminating   T He   I Nstrument

    SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.5   D econtaminating   t he   S tainer   a nd   C arousels     Figure   1 3:   L id   L atch   a nd   L ocking   P in   H ole   L ocations    ...
  • Page 60: Decontaminating   T He   R Eagent   D   B Ottle

    SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.5   D econtaminating   t he   S tainer   a nd   C arousels     Decontaminating   t he   R eagent   D   B ottle   1  ...
  • Page 61: S Hipping   O R   D Isposing   O F   T He   S Tainer   O R   C Arousels

    SECTION   5     PREVENTIVE   M AINTENANCE   A ND   S AFETY     5.6   S hipping   o r   D isposing   o f   t he   S tainer   o r   C arousels    ...
  • Page 62: Nozzle   M Aintenance   A Nd   P Erformance

    SECTION   6   NOZZLE   M AINTENANCE   A ND   P ERFORMANCE     6.1   N ozzle   D isassembly   a nd   C leaning     Nozzle   m aintenance   r equires   t he   N ozzle   M aintenance   K it   a nd   p repared   N ozzle   C leaning   Solution  ...
  • Page 63: N Ozzle   R Eassembly

    SECTION   6   NOZZLE   M AINTENANCE   A ND   P ERFORMANCE     6.2   N ozzle   R eassembly     1   Hold   y our   t humb   o r   a   s trainer   o ver   t he   e nd   o f   t he   t ube   t o   k eep   t he   n ozzle   parts  ...
  • Page 64: M Anual   P Riming

    SECTION   6   NOZZLE   M AINTENANCE   A ND   P ERFORMANCE     6.3   M anual   P riming     1   Remove   t he   c arousel   f rom   t he   b owl.    ...
  • Page 65: P Erforming   T He   V Olume   T Est

    SECTION   6   NOZZLE   M AINTENANCE   A ND   P ERFORMANCE     6.4   P erforming   t he   V olume   T est     The   V olume   T est   r equires   t he   N ozzle   M aintenance   K it.    ...
  • Page 66 SECTION   6   NOZZLE   M AINTENANCE   A ND   P ERFORMANCE     6.4   P erforming   t he   V olume   T est   9   Prepare   t he   M aintenance   K it   f or   f uture   u se:   Empty  ...
  • Page 67: F Lushing   T He   B -­-Line

      SECTION   6   NOZZLE   M AINTENANCE   A ND   P ERFORMANCE     6.5   F lushing   t he   B -­‐Line     This   p rocedure   m ay   b e   p erformed   w hen   t roubleshooting   a   s taining   i ssue.   T his   procedure  ...
  • Page 68: P Erforming   T He   S Lide   P Attern   T Est

    SECTION   6   NOZZLE   M AINTENANCE   A ND   P ERFORMANCE     6.6   P erforming   t he   S lide   P attern   T est     This   t est   c an   d ifferentiate   p oor   s taining   r esults   f rom   s ample   p reparation   p roblems,   o r   n ozzle   obstructions.  ...
  • Page 69: Solving   P Roblems

    SECTION   7     SOLVING   P ROBLEMS     7.1   T roubleshooting     This   s ection   h elps   y ou   i dentify   a nd   s olve   r outine   p roblems   w ith   t he   s tainer.   M ore   d ifficult   problems  ...
  • Page 70   S   E   C   T   I   O   N     7   SOLVING   P ROBLEMS     7.1   T roubleshooting   Table   1 3:   G eneral   T roubleshooting   a nd   D iagnosis   ( continued)   Problem  ...
  • Page 71 S   E   C   T   I   O   N     7   SOLVING   P ROBLEMS     7.1   T roubleshooting   Table   1 3:   G eneral   T roubleshooting   a nd   D iagnosis   ( continued)   Problem  ...
  • Page 72 S   E   C   T   I   O   N     7   SOLVING   P ROBLEMS     7.1   T roubleshooting   Table   1 3:   G eneral   T roubleshooting   a nd   D iagnosis   ( continued)     Problem  ...
  • Page 73 S   E   C   T   I   O   N     7   SOLVING   P ROBLEMS     7.1   T roubleshooting   Table   1 3:   G eneral   T roubleshooting   a nd   D iagnosis   ( continued)    ...
  • Page 74: A Bnormal   S Taining   R Esults

      S   E   C   T   I   O   N     7   SOLVING   P ROBLEMS     7.2   A bnormal   S taining   R esults    ...
  • Page 75: I Nstrument   M Alfunction

    S   E   C   T   I   O   N     7   SOLVING   P ROBLEMS     7.3   I nstrument   M alfunction     Air  ...
  • Page 76: Reagent   L Evel   D Etect   S Ystem   E Rrors

    S   E   C   T   I   O   N     7   SOLVING   P ROBLEMS     7.3   I nstrument   M alfunction     Reagent  ...
  • Page 77: Calibrating   T He   R Eagent   L Evel   D Etect   S Ystem

    SECTION   7     SOLVING   P ROBLEMS     7.3   I nstrument   M alfunction     Calibrating   t he   R eagent   L evel   D etect   S ystem   If   t he   R eagent   L evel   D etect   S ystem   i s   r eporting   i ncorrectly   a nd   z eroing   ( Section   2 .2)   d oes   n ot   correct  ...
  • Page 78 SECTION   7     SOLVING   P ROBLEMS     7.3   I nstrument   M alfunction   Calibrating   t he   R eagent   L evel   D etect   S ystem   ( continued)   5  ...
  • Page 79: C Alibrating   T He   T Ouchscreen

    S   E   C   T   I   O   N     7   SOLVING   P ROBLEMS     7.4   C alibrating   t he   T ouchscreen    ...
  • Page 80: C Ustomer   S Ervice

      o ffer   f ull   l ocal   s ervice   a nd   s upport.       WESCOR,   I NC   An   E LITech   G roup   C ompany   370   W est   1 700   S outh  ...
  • Page 81: Cytopro®   C Ytocentrifuge

    S   E   C   T   I   O   N     8   CYTOPRO®   C YTOCENTRIFUGE     8.1   C ytopro   C ytocentrifuge   I nformation    ...
  • Page 82: Appendix   A :   C Ritical   R Eagent   C Omponents

    APPENDIX   A     Critical   R eagent   C omponents   The   f ollowing   i nformation   i dentifies   t he   c ritical   c hemicals   o f   e ach   r eagent   u sed   i n   t his   i nstrument.   Reagent(s)  ...
  • Page 83 APPENDIX   A     Critical   R eagent   C omponents     Reagent(s)   Critical   C omponents   SS-­‐141B   G ram   I odine   C oncentrate   c ontains:   60-­‐70%   D eionized   W ater    ...
  • Page 84: Appendix   B :   R Isk   A Nd   S Afety   P Hrases

    APPENDIX   B     Risk   a nd   S afety   P hrases     Reagents   S S-­‐041AA   a nd   S S-­‐041AA-­‐EU,   G ram   R eagent   A   D ecolorizer   w ith   A cetone   a nd   S afranine   a nd   S S-­‐141A   Gram  ...
  • Page 85 APPENDIX   B     Risk   a nd   S afety   P hrases     SS-­‐041AF   a nd   S S-­‐041AF-­‐EU,   G ram   R eagent   A   D ecolorizer   w ith   F uchsin   a nd   S S-­‐141AF   G ram   R eagent   A   Fuchsin  ...
  • Page 86 APPENDIX   B     Risk   a nd   S afety   P hrases   Reagents   S S-­‐141AF   G ram   R eagent   A   F uchsin   C oncentrate   i s   a ssociated   w ith   t he   f ollowing   R isk   a nd   S afety   Phrases.  ...
  • Page 87 APPENDIX   B     Risk   a nd   S afety   P hrases     SS-­‐133   D econtamination   S olution   C oncentrate   i s   a ssociated   w ith   t he   f ollowing   R isk   a nd   S afety   P hrases.   European  ...
  • Page 88: Appendix   C :   A Ccessories   A Nd   S Upplies

    APPENDIX   C   Accessories   a nd   S upplies     Only   r eplacement   p arts   s upplied   b y   E litech   G roup   s hould   b e   u sed   i n   t his   i nstrument.   U se   o f   n on-­‐ approved  ...
  • Page 89 APPENDIX   C   Accessories   a nd   S upplies     SUPPLIES   ( continued)             REFERENCE   N UMBER   Preventive   M aintenance   C hart,   p ad   o f   2 4   s heets ........SS-­‐125   Reagent  ...

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