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Summary of Contents for Cleaver Scientific runVIEW Real-Time
- Page 1 runVIEW RealTime Horizontal System...
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Page 3: Instruction Manual
Real-Time Horizontal Electrophoresis Units Instruction Manual Catalogue Numbers CSL-RVMSCHOICE7 CSL-RVMSCHOICE15 CSL-RVMSCHOICE10 CSL-RVMSCHOCIETRIO Record the following for your records: Model _____________________ Catalogue No. _____________________ Date of Delivery _____________________ Warranty Period _____________________ Serial No. _____________________ Invoice No. _____________________ Purchase Order No. _____________________... -
Page 4: Table Of Contents
Contents Instruction Manual Catalogue Numbers Contents Safety Information Environmental Conditions Symbols Packing List Specifications Operating Instructions Overview Installation Setting up the Horizontal Gel Tank Fitting Electrodes Fitting Loading Guides Control Interface Gel Preparation Gel Pouring Casting Dams Flexicaster Tape Running the Gel Stain Compatibility Solutions References... -
Page 5: Safety Information
Safety Information The runVIEW real-time horizontal DNA electrophoresis system has been thoroughly tested and found to comply within the limits of CE regulation. It has been manufactured using the latest technology and does not require maintenance. When used correctly this unit poses no particular health risk, although it can deliver dangerous voltage levels if used incorrectly. -
Page 6: Environmental Conditions
12. Never use the power supply in the presence of flammable or combustible material as fire or explosion may result. 13. Ensure that the power supply is only connected to an earthed power line. Do not cut and splice the power line. When removing the power cord from the wall, unplug it by holding the plug attachment and not by pulling the cord. -
Page 7: Packing List
The unit should be checked for damage when received. Cleaver Scientific is liable for all missing or damaged parts / accessories within 7 days after customers have received this instrument package. Please contact Cleaver Scientific immediately regarding this issue. -
Page 8: Specifications
Specifications runVIEW Viewing Dock Blue Light 470nm Timer 1-999 minutes with alarm Wavelength Voltage/ Safety Device 25-150V / 1V No load detection Resolution Current/ Resolution 300mA / 1mA Operating Ambient to 40C Temperature Power Dimensions 30 W 293 x 220 x 80 mm Operating Mode Constant Voltage or Rated Voltage... -
Page 9: Operating Instructions
Operating Instructions Overview runVIEW is an innovative system designed for real-time size fractionation and recovery of nucleic acids. runVIEW can maximise the efficiency of DNA recovery from EtBr and SYBR stained gels by minimising the number of steps involved in post-electrophoretic purification. runVIEW consists of a multiSUB™ MSCHOICE system with bluVIEW lid, containing a spectral emission filter and extractor fan within its viewing pane, and a base unit with integrated power supply and blue LED gel illuminator. -
Page 10: Control Interface
3. Peel the back off the loading guide and carefully apply the loading guide directly to the gel platform. The unit is now ready to be used. Control Interface There are five buttons and four LED indicators on the faceplate. Each LED indicates the activation status or mode of operation of the unit. -
Page 11: Gel Preparation
Gel Preparation The Table below shows the volume of agarose solution required to make the desired agarose gel for each unit tray size. For a standard 0.7% agarose gel, add 0.7 grams of agarose to 100 ml of 1x TAE or TBE solution. The same 1X solution should be used in the tank buffer solution. -
Page 12: Gel Pouring
Gel Pouring The multiSUB range of units allows three different methods of gel casting: ® 1. Casting Dams 2. Flexicaster 3. Traditional Tape Casting Dams 1. To fit the casting dams, place one casting dam on the bench with the groove facing upwards (1). -
Page 13: Tape
2. Insert the desired length tray into the Flexicaster such that one end of the tray is pushed up and seals against the silicone mat of the permanent end of the Flexicaster. 3. Position the movable end of the Flexicaster so that the silicone mat is pushed against the other end of the tray. -
Page 14: Running The Gel
and care must be taken to ensure that samples from the first wells do not migrate into the lanes of the second comb wells. 3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur can be smoothed to the edge of the gel and dispersed using a pipette tip. -
Page 15: Stain Compatibility
Stain Compatibility Staining Method Nucleic Acid Relative Performance Gel Pre- Gel Post- Sample Stain Between Stains Staining Staining Staining SYBR® Green I Higher Intensity Bands (DNA) Observed SYBR® Green II Higher Intensity Bands (RNA) Observed SYBR®... -
Page 16: Solutions
Solutions 0.5M EDTA stock (500mL) dissolve in 400 ml distilled water: 93.05g EDTA disodium salt Fill to 500 ml litre final volume with distilled water 50X TAE stock (1L) dissolve in 750 ml distilled water: 242 g tris base (FW = 121) ... -
Page 17: Troubleshooting
Some suggestions for troubleshooting are given below. If these suggestions fail to resolve the problem, contact support@cleaverscientific.com or the Cleaver Scientific distributor in your region for assistance. If troubleshooting is required, please include a full description of the problem. -
Page 18: Runview Issues
Press START/STOP button to clear the error Over voltage (170V safety limit reached or message. Contact Cleaver Scientific’s service exceeded). department if the problem persists. Error message No load is detected. (1) Check the connections. -
Page 19: Care And Maintenance
If the unit is to be returned for repair, contact support@cleaverscientific.com or your local authorised Cleaver Scientific distributor. Cleaning Horizontal Units Units are best cleaned using warm water and a mild detergent. Water at temperatures above 60°C can cause damage to the unit and components. -
Page 20: Ordering Information
Ordering information Ordering Information CSL-RVMSCHOICE7 runVIEW system complete with 15 x 7cm gel tray. CSL-RVMSCHOICE10 runVIEW system complete with 15 x 10cm gel tray. CSL-RVMSCHOICE15 runVIEW system complete with 15 x 15cm gel tray. CSL-RVMSCHOICETRIO runVIEW system complete with 15 x 7, 15 x 10 and 15 x 15cm gel trays. CSL-RVBSBVLID runVIEW base station &... - Page 21 MS15-28MC-1.5 Choice MS15-35-1.5 Choice MS15-1-2 Choice MS15-2-2 Choice MS15-4-2 Choice MS15-10-2 Choice MS15-10MC-2 Choice MS15-12-2 Choice MS15-14MC-2 Choice MS15-16-2 Choice MS15-20-2 Choice 4.75 MS15-28MC-2 Choice MS15-35-2 Choice 15/11/2018 Page 19...
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Page 22: Related Products
Related Products Catalogue No. Product description nanoPAC-300 Mini Power supply, 300V, 400mA, 60W -100 -240VAC nanoPAC-500 Mini Power supply, 500V, 400mA, 120W -100 -240VAC POWERPRO300 MIDI Power Supply, 300V, 700mA, 150 – 100 -240VAC OMNIDOC plus Blue LED Epi-illumination Module (OMNIDOC-BL), and omniDOCPROSAFE 520, 560 &... -
Page 23: Warranty
Warranty The Cleaver Scientific Ltd. units have a warranty against manufacturing and material faults of twelve months from date of customer receipt. If any defects occur during this warranty period, CSL will repair or replace the defective parts free of charge.
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