Sutter Instrument dPatch SutterPatch Quick Start Manual page 2

QUICK START GUIDE
DIGITAL PATCH CLAMP AMPLIFIER SYSTEMS OVERVIEW
Please read these instructions carefully before installation. This guide covers both
the dPatch and dPatch-2 systems. If you have any questions or need additional
®
information, contact Sutter Instrument.
OPERATING SYSTEM REQUIREMENTS
Windows 10: 64-bit versions
macOS 10.11 (El Capitan) or later
INSTALL HARDWARE
1. Attach dPatch headstage(s) to amplifier front panel: HEADSTAGE 1 & 2. dPatch
headstages can be attached to any headstage port; they are not "matched" to
a port.
2. OPTIONAL: Attach dPatch Screw Terminal Board to amplifier rear panel
-> DIGITAL OUTPUTS, or dPatch Expansion Panel to DIGITAL OUTPUTS &
AUXILIARY I/O.
3. Attach USB cable to USB 3.0 port on amplifier rear panel and your computer.
The connector on the dPatch rear panel must click in place.
4. Attach power cord to amplifier rear panel and a grounded power outlet.
5. Power on the amplifier.
INSTALL IGOR PRO / SUTTERPATCH SOFTWARE
1. Power on computer.
2. If you have internet access, download the latest version of the SutterPatch
software installer from www.sutter.com/AMPLIFIERS/SutterPatch.html
3. If internet access is not available, attach the included USB flash drive to your
computer.
4. Double-click on 'sutterpatch_win_full' (Windows) or 'sutterpatch_mac_full'
(macOS).
5. Follow the installer prompts for current Igor software.
6. Open Igor Pro and activate the Igor Pro license as instructed:
i. Serial Number:
ii. Activation Key:
affix label here
TEST SYSTEM
1. Model Cell:
a. Attach Model Cell to HEADSTAGE 1 and tighten the collar.
b. Attach ground wire to HEADSTAGE 1 and Model Cell (gold plugs).
c. Surround with aluminum foil shielding and ground it to the headstage
ground connector.
2. Click icon to launch Igor:
SUTTER INSTRUMENT
3. SutterPatch welcome screen will open. Press 'Start'.
4. On dashboard window:
I. Click on the Acquire Data icon:
Acquire Data
II. Click on the Membrane Test icon:
Membrane Test
5. Scope + Analysis windows open:
(Actual analysis values depend on dPatch filter settings)
I. With the Model Cell switch in Bath position, click the Bath button.
Pipette Resistance = ~10 MΩ
II. With the Model Cell switch in Seal position, click the Seal button.
Seal Resistance > 10,000 MΩ
III. With the Model Cell switch in Cell position, click the Cell button.
Access Resistance = ~10 MΩ
Membrane Resistance = ~500 MΩ
Membrane Capacitance = ~28 pF
Move the Model Cell and shielding to HEADSTAGE 2 (if installed), set the Scope
window to 'Headstage 2', and repeat steps I–III.
BACK PANEL
DIGITAL OUTPUTS 1-16
19
37
DIGITAL OUTPUTS PINOUT
PIN FUNCTION
1 - 16 Digital Output 1 - 16
17 - 19
20 - 35
36 - 37
dPATCH
1
20
+5 V
Ground
+5 V
DIGITAL PATCH CLAMP AMPLIFIER SYSTEM
/ dPATCH-2
®