RotaChrom rCPC Operation Manual

Hide thumbs

Table Of Contents

Table of Contents

Quick Links

Download this manual

RotaChrom

rCPC

Operation Manual

Version number: OPM-rCPC-121818

Reviewed by Attila Babits

Issued by Zsuzsanna Friedman

RotaChrom Technologies LLC

151 Fő út

Dabas, 2370, Hungary

Table of Contents

Need help?

Do you have a question about the rCPC and is the answer not in the manual?

Questions and answers

Summary of Contents for RotaChrom rCPC

Page 1 RotaChrom rCPC Operation Manual Version number: OPM-rCPC-121818 Reviewed by Attila Babits Issued by Zsuzsanna Friedman RotaChrom Technologies LLC 151 Fő út Dabas, 2370, Hungary...
Page 2 Operation Manual rCPC OPM-rCPC-121818 ©2018 RotaChrom Technologies LLC All Rights Reserved. All other trademarks are the property of their respective owners. Find us on the World Wide Web at: rotachrom.com RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 3 OPM-rCPC-121818, December 2018 Software version 1.0 This document was created to: ■ Replace the previous Operation Manual ■ Improve the content and usability of the instructions ■ Update the Hazards section RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 4 The customer is responsible for routine preventive maintenance procedures. The cost of repairs, arising from the failure to perform these maintenance procedures at the indicated time intervals according to the specifications provided by RotaChrom, is not covered in the warranty; therefore, it is the expense of the costumer.
Page 5 Follow the power requirements in the system specifications. Follow the procedures and safety warnings throughout this manual. If you use the system in a manner not specified by RotaChrom, the protection provided by the system can be impaired or system failure can occur.
Page 6 Some substances in the chemical preparations may have different storage and disposal regulations. Follow your operation procedure that corresponds the regulations of your country. rCPC Hazards Only a RotaChrom representative can install the system. If the system installation needs modification, please contact RotaChrom. ■...
Page 7 ■ Dedicate the computer hardware to only running the system software. Do not connect the computer hardware to the Internet, unless instructed to do so by RotaChrom. ■ Keep the system’s safety coverings and top coverings closed except for startup procedures and maintenance.
Page 8: Table Of Contents
Electrical and communication cable disconnection: ..............34 Rotary union replacement: ......................34 Spare parts ............................36 Warranty ............................... 37 Troubleshooting ............................ 37 General Safety Information ........................38 General .............................. 38 Operation ............................38 RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 9: Introduction
Introduction RotaChrom rCPC is a research & development purpose Centrifugal Partition Chromatography system intended for pilot scale purification of compounds. Centrifugal Partition Chromatography is a liquid- liquid chromatographic technique, where both stationary and mobile phases are liquids, and the liquid stationary phase is immobilized by a strong centrifugal force.
Page 10: Safety Precautions
This manual contains the operating instructions of the rCPC system, as well as cleaning instructions and maintenance instructions. 3. figure: rCPC with connected vessels Safety precautions Be aware that all covers (including front door) should be mounted before plugging instrument into the mains outlet.
Page 11: Instrument Installation
4. figure: RotaChrom rCPC cells in holder Instrument installation RotaChrom rCPC system installation is always carried out by qualified personnel trained by RotaChrom or its partners. User may disconnect some connections and reinstall them by the instructions described in this chapter.
Page 12 1/8” SS tubing, with VICI nut and SS/PEEK ferrule at both ends. ASC/DSC valve should be connected to the rotor with 1/8” SS tubing, with VICI nut and SS/PEEK ferrule at valve end and Swagelok nut and ferrule at rotor front (DSC). RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 13 Detector should be connected to fraction collector with 1/8” FEP or PEEK tubing, with PEEK nuts and inverted ferrules. Fraction collector has 10 output connections, recommended tubing is 1/8” FEP or PEEK with PEEK nuts and inverted ferrules. 11. figure: Fraction collector RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 14 There is a Swagelok 1/8” tubing to ¼” G thread adapter, with SS304 seal. Check the connection tubing for bending. Check the interconnects for proper connection and fixing. 13. figure: Rotary seals and interconnects RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 15: Starting Of The Instrument
Starting of the instrument Connect the instrument to mains AC and turn on the mains switch. 15. figure: Mains switch RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 16 +36-70-885-6922 for registration key. (Normally the registration is done by RotaChrom during the installation.) After you execute ECOMAC application you can see next window or similar one. It is the main window running as Station 1. Main part of the window is the graph where application shows measured data.
Page 17 You can add selected device to the right by pressing Add button. Devices on the right side are those which application should connect at start time (initiation phase). Check the list for main components of rCPC instrument (CPC, ECP pump, Flash detector and Vici actuator /spider). 19. figure: Device identification RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 18 After communication tests click on Control button. Time table allows doing available actions in specified time of analysis. Use right click on area under preview graph to manipulate with this table. 21. figure: Method setup RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 19: Normal Operation Of The Instrument
Start – startup of the instrument (detector wavelength, filling with stationary phase, spin up of rotor) ■ Loop – injection cycle (injection, elution, fraction collection, extrusion) ■ Stop – stopping of the instrument RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 20: Method Creating In The Software
Method creating in the software Before the method creating the operator prepared and connected the solvents and the dissolved sample to the rCPC. The gradient box inlet connections are the next: • connect the mobile phase to the A line •...
Page 21 Fourthly, the operator has to write a sequence. Here inserts the start, loop and stop method. If the operator wants more injection during the run insert more than one loop method in the sequence. RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 22 It is highly recommended to have flow through the rotor when it is spinning. Running the rotor at high speed without flow decreases rotary seal lifetime. RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 23: Cleaning And Maintenance
Cleaning and maintenance Cleaning RotaChrom rCPC is used in a laboratory or in pilot scale production, as so requires attention during handling of solvents and solutes, as well as occasional cleaning. During a spillage or leak it is recommended to stop the instrument, shut it down and clean it right away.
Page 24: Cell Assembly After Cleaning
14 mm pipe wrench and fix with a torque of 11 Nm. Do not use standard wrench and make sure pipe wrench firmly covers all of the plug. RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 25 29. figure: RotaChrom rCPC cell assembly After connecting plugs screw in the cell in to the cell holder block (6). It is recommended to fix the holder temporary to a stand. Fix with torque of 19-20 Nm. Connect the cells in-line with „C” type (7) interconnect tubing.
Page 26 Cell blocks should be screwed into the rotor, on a stand. Interconnects between cell blocks should be made with „S” type interconnects (10). Input and Output interconnect should be screwed before putting on rotor cover and putting rotor inside machine. RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 28: Maintenance And Service
Rotor should be touched on the cells and rotor plates, but not on the interconnects! Extensive force on interconnects bends them or even brakes the plugs. It is advised to ask RotaChrom’s personnel for rotor removal/mounting and/or use the dedicated device for rotor removal/mounting.
Page 29 With the rotor holding tool (or carried by two workers) move the rotor close to the axis and check the wedge to be in the correct position. 34. figure: mounting of rotor RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 30 35. figure: inlet and outlet connections on the front and back side of instrument output tube (10, 19) hex adapter (11), rotary seal (13), screw (35, 33, 12), tube holder (32), nut (21), slot for screw (11, 13) and input (7) connectors RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 31: Pump Seals And Check Valves Replacement
Loosen screws in head using TX20 screwdriver from unit accessories. Pull the head straightly out of the piston. Leaning the head to sides may cause piston damage! Detail of working head. RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 32 7 MPa. Let the pumping for at least 10 min (optimally 30 min.). Then set flow rate at 30 ml/min. and pressure at 2 MPa. Let the pumping for at least 10 min (optimally 30 min). Seals are now RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 33: Detector Lamp Replacement
Never touch the quartz bulb with bare fingers because fingerprints will irreversibly damage the lamp after being switched on. Clean any dust with air duster. RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 34: Electrical And Communication Cable Disconnection
Electrical and communication cable disconnection: At first the rCPC must be switched off and the electrical cable must be pulled out. Connectors on Unit Rear Panel Unplug the power supply cord into the instrument socket on the rear panel. Unplug serial line cable into connector RS232 for interconnection with PC.
Page 35 39. figure: Replaced rotary union The process of the back rotary union replacement is the same. The terms of service are provided on the basis of the service guideline. RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 36: Spare Parts
Spare parts The RotaChrom rCPC incorporates many wear parts, which has defined exchange interval for preventative maintenance. The following table summarizes user exchangeable wear parts. However, we do recommend exchange carried out by RotaChrom personnel for maximal performance. Unit Part Interval 3/16”...
Page 37: Warranty
Fraction collection not working Loosened electric connector Check connection on the back of the fraction collector Noise in signal Check for interference sources Valve fail Contact support Other Unspecified Contact support RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...
Page 38: General Safety Information
Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument. Rotachrom assumes no liability for the customer’s failure to comply with these requirements. Ensure the proper usage of the equipment.
Page 39 In case of emergency stopping the operator has two possible choices: Hardware emergency stop: press the red button on the rCPC left side and unplug the wall electrical connection 40. figure: Emergency stop Software emergency stop: Firstly, click the stop icon at the top of the window.
Page 40 Secondly, switch off the rotation and after the pump on the on-line configuration side. 42. figure: Switch off the rotation 43. figure: Switch off the pump RotaChrom rCPC Operation Manual All Rights Reserved by RotaChrom Technology LLC©...