New England BioLabs NEBNext Ultra II Instruction Manual

End repair/da-tailing module

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INSTRUCTION MANUAL
NEBNext
®
Ultra™ II End Repair/dA-Tailing Module
NEB #E7546S/L
Table of Contents
Protocol ..................................................................................................................................................................................... 2
Kit Components ........................................................................................................................................................................ 2
Revision History ....................................................................................................................................................................... 3
The NEBNext Ultra II End Repair/dA-Tailing Module Includes
The volumes provided are sufficient for preparation of up to 24 reactions (NEB #E7546S) and 96 reactions (NEB #E7546L). All reagents
should be stored at –20°C. Colored bullets represent the color of the cap of the tube containing the reagent.
(green) NEBNext Ultra II End Prep Reaction Buffer
(green) NEBNext Ultra II End Prep Enzyme Mix
The NEBNext Ultra II End Repair/dA-Tailing Module is Designed for use with the Following:
NEBNext Ultra II Ligation Module (NEB #E7595)
®
NEBNext Ultra II Q5
Master Mix (NEB #M0544)
NEBNext Singleplex or Multiplex Oligos for Illumina
(NEB #E7350, #E7335, #E7500, #E7600 or #E7535)
Applications
The NEBNext Ultra II End Repair/dA-Tailing Module is optimized to convert 500 pg-1 µg of fragmented DNA to repaired DNA having 5´
phosphorylated, 3´ dA-tailed ends.
Lot Control: The lots provided in the NEBNext Ultra II End Repair/dA-Tailing Module for Illumina are managed separately and qualified
by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet
stringent criteria in the additional quality controls listed on each individual component page.
Functionally Validated: Each set of reagents is functionally validated together through construction and sequencing of an indexed DNA
library on the Illumina sequencing platform.
For larger volume requirements, customized and bulk packaging is available
by purchasing through the OEM/Bulks department at NEB. Please contact OEM@neb.com for further information.
®
24/96 reactions
Version 3.0_3/20
1

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Summary of Contents for New England BioLabs NEBNext Ultra II

  • Page 1 (NEB #E7350, #E7335, #E7500, #E7600 or #E7535) Applications The NEBNext Ultra II End Repair/dA-Tailing Module is optimized to convert 500 pg-1 µg of fragmented DNA to repaired DNA having 5´ phosphorylated, 3´ dA-tailed ends. Lot Control: The lots provided in the NEBNext Ultra II End Repair/dA-Tailing Module for Illumina are managed separately and qualified by additional functional validation.
  • Page 2 If necessary, samples can be stored at –20°C; however, a slight loss in yield (~20%) may be observed. We recommend continuing with adaptor ligation before stopping. 1.4. Proceed directly to NEBNext Ultra II Ligation Module (NEB #E7595) Kit Components NEB #E7546S Table of Components...
  • Page 3 This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information about commercial rights, please email us at gbd@neb.com While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain...

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