Harvard Bioscience CMA 12 Elite User Manual

CMA 12 Elite Microdialysis Probe User's Manual
Instructions for CMA 12 Elite Microdialysis Probe
1. Fill a microsyringe with perfusion fluid and mount it in the CMA Syringe Pump. The Perfusion Fluid must be clean,
at room temperature and preferably degassed.
2. Run the pump to make sure that liquid leaves the tip of the syringe cannula
3. Connect the desired length of tubing to the inlet and outlet of the probe. Short cannula = inlet, long cannula = outlet.
Tubing Adaptors and FEP Tubing should be used for all connections. To facilitate the handling of Tubing Adaptors,
they should be pre-soaked in ethanol for a minimum of 15 minutes.
4. Mount the microdialysis probe in the CMA 11 & 12 Probe Clip on the CMA/130 In-Vitro Stand or the In-Vitro holder
on the CMA 402 Pump. Put the microdialysis probe in a vial filled with perfusion fluid.
5. Connect the inlet tubing of the microdialysis probe to the syringe cannula, by sliding the Tubing Adapter over the
cannula. Wait for 10 min as the Tubing Adapter must be dry before flushing.
Flush the probe with 10-15 μL/min in the Perfusion Fluid for 4-5 min to wash out air. While flushing, "tap" with a scissor
6.
on the probe clip (not the probe) to remove air bubbles. The vibrations from the probe clip will in most cases remove the
air bubble. If possible, check for air bubbles under a stereomicroscope. If the air bubble is not gone, the flushing and
"tapping" must be repeated. When flushing the membrane it may appear to be "sweating" which is due to ultrafiltration
of fluid through the membrane.
Set the pump to the required perfusion flow (usually 1-5 μL/min) and check for leaks. The microdialysis probe is now
7.
ready for use.
When changing sample vials, remember to consider the internal volume in the system (see TECHNICAL
8.
INFORMATION). This causes a delay that must be calculated when using low perfusion rates and short sampling
times.
After the experiment, put the microdialysis probe in a vial filled with deionized water. Perfuse with deionized water to
9.
prevent salt crystal formation. The probe can be stored in deionized water.
10. For further set up instructions, see CMA 120 System for Freely Moving Animals, User's Manual.
TECHNICAL INFORMATION
Membrane
Material
Molecular Cut-Off
Outer Diameter
Length
Probe Shaft
Material
Diameter
Length
Internal Volume
Inlet Volume
Outlet Volume
Polyarylethersulfone (PAES)
20,000 Daltons
0.5 mm
1, 2, 3 and 4 mm
Stainless-steel or Metal Free
0.64 mm
14 mm
0.2 µL
2.6 – 2.9 µL