Related Manuals for Asymptote EF600 Series
Summary of Contents for Asymptote EF600 Series
- Page 1 Asymptote Ltd St John's Innovation Centre Cowley Road Cambridge CB4 0WS Tel: +44 1223 421161 Fax: +44 1223 421166 e-mail: info@asymptote.co.uk EF600 Series Instruction Manual Asymptote nitrogen-free controlled rate freezer...
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Page 2: Table Of Contents
UMAN SPERMATOZOA CRYOPRESERVATION LINEAR COOLING 6.6 H – ....20 UMAN SPERMATOZOA CRYOPRESERVATION LINEAR COOLING 7. REFERENCES ....................21 Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... - Page 3 NSTALLING THE PROGRAM APPENDIX 3: UNINTERRUPTIBLE POWER SUPPLY (UPS) ......29 APPENDIX 4: CRYOPEN NUCLEATING TOOL OPERATION ...... 29 Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk...
- Page 4 Figure A2.6 – Installation box 6 .................. 27 Figure A2.7 – Installation box 7 .................. 27 Figure A2.8 – Installation box 8 .................. 28 Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk...
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Page 5: Introduction
Additional programs, up to a total of 10, may be specified at the time of ordering. This manual includes operating instructions for all three versions. The following optional extras may also be supplied with the EF600 series freezer: Cryopen nucleating tool (EF-611) Uninterruptible power supply (UPS) (EF-613) -
Page 6: Warning
Plate temperature The plate temperature should never exceed 30ºC. If it does, turn the freezer off immediately. Maintenance A service contract on the EF600 series freezers is available. Contact info@asymptote.co.uk for full details. Please note that there are no user serviceable parts within the EF600 and that breaking the security tags invalidates any warranties. -
Page 7: Installing The Freezer Unit
Installing the Freezer Unit Unpacking Carefully unpack the Asymptote EF600 controlled rate freezer from its carton and place upon a suitable bench. Only lift the freezer by placing hands underneath the freezer; do not move the freezer by pressing on the casework. - Page 8 Figure 2.4 – Front panel of freezer unit (keypad and ON/OFF switch) Starting Asymptote’s Data Logging Software (EF600-01, EF600-10) Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk...
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Page 9: Figure 3 - Desktop Shortcut Icon
EF600 Instruction Manual Start the software by either double-clicking on the shortcut icon on your desktop (figure 3) or by selecting ‘Asymptote Data Logger’ from the program list in the start menu on your computer. Figure 3 – Desktop shortcut icon The program window will open. -
Page 10: User Interface (Ef600-01, Ef600-10)
Figure 4 – User interface Selecting the logging interval (EF600-01, EF600-10) and freezing profile Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 11: Log File Names (Ef600-01, Ef600-10)
Choose logging Choose profile interval (EF600-10) (EF600-01, EF600-10) Figure 4.1 – Freezing profile Log file names (EF600-01, EF600-10) Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 12: Figure 4.2 - Log File Names
Log File Name Figure 4.2 – Log file names Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 13: Operating Procedure For The Ef600 Freezer
Position the straws into the sample plate and fully press them into the grooves (figure 5.4). Place the lid over the sample plate checking that it is located Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 14: Starting The Data Logging (Ef600-01, Ef600-10)
Note that the ‘Ready To Load’ light may remain on for a short time at the Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 15: Sample Nucleation
When the cycle is complete the yellow ‘Cycle Complete’ light will illuminate. The straws can now be removed from the freezer and placed in storage. The Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 16: Stopping The Data Logging
The surface of unit and the sample plate can be wiped down with a sterile filtered Biocide C wipe. Dry the sample plate thoroughly after cleaning. Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 17: Freezing Cycles
00:40 01:00 01:20 01:40 02:00 Time (hh:mm) Figure 6.1 – Pre-programmed human embryo freezing cycle Human oocyte cryopreservation Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 18: Human Blastocyst Cryopreservation
01:20 01:40 02:00 Time (hh:mm) Figure 6.3 – Pre-programmed human blastocyst freezing cycle Human embryonic stem cell cryopreservation Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 19: Human Spermatozoa Cryopreservation - Linear Cooling
-100°C -100 00:00 00:10 00:20 00:30 00:40 00:50 Time (hh:mm) Figure 6.5 – Pre-programmed human sperm freezing cycle Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 20: Human Spermatozoa Cryopreservation - Non-Linear Cooling
-100°C -100 00:00 00:10 00:20 00:30 00:40 Time (hh:mm:ss) Figure 6.6 – Pre-programmed human spermatozoa non-linear freezing cycle Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 21: References
Morris GJ, Acton E, and Avery S (1999) A novel approach to sperm cryopreservation. Human Reproduction 14 1013-1021 Appendix 1: Troubleshooting Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 22: A1.1 Freezing Cycle Will Not Start
Check ON/OFF switch is illuminated and switch to ON position if necessary. A1.4 Straws stuck to sample plate Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... - Page 23 Dry straws before freezing (section 6.3). Note that the grooves fit tightly around the straws such but it should always be possible to remove the straws using tweezers. For help with any other problems, please contact Asymptote Ltd: Asymptote Ltd St John’s Innovation Centre...
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Page 24: Appendix 2: Installing Asymptote's Data Logging Software
CD using Windows Explorer and double click on ‘Setup.exe’ to start. Asymptote’s software requires ‘.NET Framework 1.1’ or later to run. The best way to obtain the latest version of this update is to visit the windows update site and scan for ALL available updates. -
Page 25: Figure A2.2 - Installation Box 2
1.1’. If this update has already been installed on your computer, select ‘Next’ to move to the next window. Figure A2.3 – Installation box 3 Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 26: Figure A2.4 - Installation Box 4
Select ‘Next’ to confirm the installation or ‘Cancel’ to exit. This is the final step before the program is installed. Figure A2.5 – Installation box 5 Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 27: Figure A2.6 - Installation Box 6
When the installation has finished, Installation box 7 will be displayed. Select ‘Close’ to exit. Figure A2.7 – Installation box 7 Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 28: Figure A2.8 - Installation Box 8
The final indication that installation has been successful will be a small message box saying ‘Setup succeeded’: Figure A2.8 – Installation box 8 Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk... -
Page 29: Appendix 3: Uninterruptible Power Supply (Ups)
To nucleate, remove the cryopen’s protective cap, touch the end of the cryopen to the straw and press the lever down for approximately 1 second (figure 5.7). Asymptote Ltd. St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk...
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