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Summary of Contents for Plexon Optogenetic Controller
- Page 1 Optogenetic Controller 6500 Greenville Ave. Suite 700 Plexon Inc Dallas Texas 75206 United States of America Tel: 214-369-4957 Fax: 214-369-1775 E-mail: info@plexon.com www.plexon.com Stimulator 2.0 Optogenetic Controller December 2012 April 2011...
- Page 2 CAUTION: Plexon Inc Proprietary The information contained herein is the property of Plexon Inc. and it is proprietary and restricted solely to assist Plexon Inc. customers. Neither this document nor the contents may be disclosed, copied, revealed or used in whole or in part for any other purpose without the prior written permission of Plexon Inc.
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Page 3: Table Of Contents
5.2.2 Connect the DC power cord between the power supply and the Optogenetic Controller................................11 5.2.3 Connect the USB cable between the Optogenetic Controller and the computer. 11 5.2.4 Connect the BNC cable between the Optogenetic Controller channel 1 and the Turning on the power for the first time .................. - Page 4 Edge Triggering ..........................23 7.7.2.2 Level Triggering ..........................23 7.7.2.3 DI Polarity Positive ........................23 File Load Settings / File Save Settings ..................... 24 7.7.2.4 DI Polarity Negative........................24 View ................................24 7.10 Window ................................ 24 Optogenetic Controller - 4 -...
- Page 5 Sample Arbitrary Waveform Pattern Files ..........27 10.1 Latency between digital input and voltage output ..............28 Optogenetic Controller Limitations ............28 10.2 Synchronization of multiple Controllers ..................29 10.3 LED Flashes when changing from voltage to current mode ..........30 Specifications ..................
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Page 6: Getting Started
Thank you for purchasing this Plexon product. We hope you are pleased with every aspect of it. Please do not hesitate to contact us if you have any questions. -
Page 7: System Components
Neurotechnology Research Systems 4 System components When you receive your Plexon Optogenetic Controller, confirm that you have the following pieces: USB memory with software & drivers Plexon AC power cord (7.5 ft)* Volex 17250 10 B1 Power Supply Plexon 08-06-A-37... -
Page 8: Installation
If the software is already installed, then the welcome screen gives you the option to repair (re-install) or remove the software as shown on the right above. If the software is not already installed, then the welcome screen shown above on the left appears. Click Next to continue. Optogenetic Controller - 8 -... -
Page 9: Read And Accept The Plexon End User License Agreement
Neurotechnology Research Systems Read and accept the Plexon End User License Agreement 5.1.4 Click Next to Continue You will be asked to confirm where the files will be stored: 5.1.5 Click Next to continue. When the installation begins, it may take a couple of minutes before the progress bar begins moving across the window. -
Page 10: Accept The Opal Kelly License Agreement
Accept the Opal Kelly License Agreement 5.1.8 Click Finish When the Opal Kelly Driver Setup is complete. 5.1.9 5.1.10 Click Finish in the Plexon Radiant installation window 5.1.11 The computer will have to be restarted to complete the installation. Optogenetic Controller - 10 -... -
Page 11: Hardware Installation
An oscilloscope is highly recommended for viewing the output of the channels in voltage mode. Connect the AC power cord between the AC outlet and the power supply. 5.2.1 Connect the DC power cord between the power supply and the Optogenetic Controller. 5.2.2 Connect the USB cable between the Optogenetic Controller and the computer. -
Page 12: Turning On The Power For The First Time
5.3 Turning on the power for the first time Flip the power switch to the on position. The LED next to the switch on the end of the Optogenetic Controller should illuminate. 5.3.1 The first time you turn the stimulator on a “Found New Hardware” balloon will appear in the lower right hand corner of the computer screen. -
Page 13: Getting Started
Neurotechnology Research Systems 6 Getting Started This section introduces the basic functions of the Optogenetic Controller and guides you through basic operation using the voltage mode. Voltage mode is intended to be used to control lasers with a 0-5V control input. The current mode functions similarly for controlling LEDs, but it is more difficult to quantify the output without a light meter. -
Page 14: Operational Flowchart
Set LED or LASER Mode Set Max Curent, Voltage, or Power Manual File Pattern Pattern Enter Desired Current or Browse to File Voltage No File Header With Header Enter Info Set Number of Repetitions Start Stimulation Pattern Optogenetic Controller - 14 -... -
Page 15: Launching The Radiant Software
If it is not in manual mode, you can select it by clicking the drop down menu next to Pattern Source. © Plexon Inc - 15 -... -
Page 16: Starting And Stopping Stimulation
In hardware there are start and stop TTL-compatible digital inputs for each channel. Inputs should range from 0 – 5 V. Inputs <0.8V are logic “0” and >2.0V are logic “1”. The pinout for the Digital In connector is shown below. Optogenetic Controller - 16 -... - Page 17 1 in level mode. This is the more common method of communicating through TTL. In Negative mode, the controller responds to a falling edge if the inputs are in edge mode, or to a logic © Plexon Inc - 17 -...
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Page 18: Defining A Pattern With A .Txt File
A dialog box will pop up. There are two types of files (.opt and .txt). A .opt file has a header that defines stimulation parameters such as units, while a .txt file is just a series of amplitudes. Optogenetic Controller - 18 -... - Page 19 Neurotechnology Research Systems Select the file pulse_bursts.txt that is located at C:\Plexon Data\Sample Pattern Files. Note that the pattern files are Read Only. To change them, first copy and rename the original and then remove the Read Only flag by right clicking the file name and selecting Properties from the drop down. One of the dialog boxes below will appear depending on whether LASER or LED is selected for the Output Device.
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Page 20: Verifying The Output On An Oscilloscope
Note that the scaling is 100mV per division and 1 second per division. 6.9 Using Different Scaling Options The Optogenetic Controller fundamentally outputs either a 0-5V signal to control a laser or a 0- 1100mA signal to directly power an LED. The Radiant software allows patterns to be specified in voltage or current, but some users may prefer to specify their stimulation pattern in terms of light output. -
Page 21: Gui Function Reference
(mW). If Power is selected, the power delivered at the Current Output Limit must be manually entered. Note that when using one of the pre-configured Plexon LED modules, the current limit is shown next to the wavelength in the LED Type window. -
Page 22: Current Or Voltage Limit
To reduce the risk of damaging your LED, the current limit can be set. There are default values for Plexon’s LED modules that can be selected, or a custom value can be entered. Reducing the current limit also has the additional benefit of increasing your resolution because the output is an 8 bit value ranging from 0 to the current limit. -
Page 23: Trigger Options
If a digital input is at 0V and the polarity is changed from positive to negative, stimulation will start. © Plexon Inc - 23 -... -
Page 24: File Load Settings / File Save Settings
LED number that is illuminated under “Device” on the end panel of the controller. 7.10 Window You can adjust the appearance of the Radiant GUI in the same ways that other Plexon software packages can be adjusted. Each window can be resized, docked, undocked, or hidden completely. -
Page 25: Input And Output Connectors
This information is provided for reference only. Use the recommended power supply and attached cable provided by Plexon to power the controller. The power input connector is a locking 2.5 mm inner / 5.5 mm outer barrel connector. The stimulator operates from a grounded 12V power supply. -
Page 26: Current Or Voltage Output Connectors
So if the pattern is a series of pulses, the LED will be on during the pulse but off in between pulses. It mirrors the output on the non-zero digital output pin. Optogenetic Controller - 26 -... -
Page 27: Running
This means that to fully know the timing of a pattern, multiple digital outputs of the optogenetic controller need to be monitored. The rising edge of the running signal indicates a pattern has started and the rising edge on the stop signal indicates the pattern has finished. -
Page 28: Optogenetic Controller Limitations
Neurotechnology Research Systems Optogenetic Controller Limitations 10.1 Latency between digital input and voltage output The timing dynamics in voltage mode of starting the first channel with a digital input are shown below. All digital outputs change to reflect that a digital input trigger has been detected within 200ns, though not exactly at the same time. -
Page 29: Synchronization Of Multiple Controllers
(assuming one clock is 50 ppm fast and one is 50 ppm slow). This is a worst-case scenario, but there will certainly be some drift between different controllers. © Plexon Inc - 29 -... -
Page 30: Led Flashes When Changing From Voltage To Current Mode
4ms. The easiest way to avoid this is to switch to current mode before connecting the LED to the controller or before connecting the patch cable to the LED module. Optogenetic Controller - 30 -... -
Page 31: Specifications
TTL, Low < 0.8V, High > 2.0V Digital output levels HCT, Low < 0.33V, High > 3.84V Digital input/output latency See section Minimum trigger pulse width 200 ns Update rate 10 kHz Arbitrary waveforms © Plexon Inc - 31 -... - Page 32 6500 Greenville Ave. Suite 700 Plexon Inc Dallas Texas 75206 United States of America Tel: 214-369-4957 Fax: 214-369-1775 E-mail: info@plexon.com www.plexon.com Optogenetic Controller December 2012...
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