Unpacking the equipment ..................16 Installing the freezer ....................17 Installing a Dura-Lo or Dura-Tech Cylinder ......................... 18 Installing a Planer Pump and Dewar ................18 2.5.1 Attaching the delivery tube ..................19 2.5.2 Putting liquid nitrogen into the dewar ..............19 2.5.3...
Hard copies and backup copies should be routinely kept. All equipment supplied by Planer Limited is subject to a Warranty Agreement and Limited Liability. In addition, software products are not sold but are licensed for use and are subject to a Licence Agreement.
Introduction to the Integra 750Plus This indicates first-aid advice. 1.2.2 Symbols used on the freezer Warning: consult the manual and the accompanying documentation. Mains supply ON Mains supply OFF Alternating current RUN key STOP key HEAT manual override COOL manual override Caution —...
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Use tongs or wear cryogloves when handling cold or hot objects. Cryogloves are available as an accessory from Planer. When running a program, the chamber and contents may get cold enough to cause frostbite or hot enough to cause burns.
The AUX socket on the Controller is to be used only for calibration by qualified service personnel. It must not be connected to any other equipment. · The Sample PRT socket inside the chamber is only to be used with a Planer Sample PRT. ·...
Introduction to the Integra 750Plus About this Manual Welcome to Integra 750Plus, a system that provides precision cooling and heating of biological specimens. This manual is aimed at Integra users; that is, people who are responsible for operating or installing the Integra system.
1.6.1 The Integra 750Plus freezer The Integra 750Plus freezer has many important design features: Precision temperature control - Accurate linear cooling and heating is achieved by the controlled injection of atomised liquid nitrogen into the chamber and the pulsing of a heater.
Alternatively, a Planer 5psi dewar and pump can be used. The dewar contains the store of liquid nitrogen, and the pump is inserted into the neck of the dewar. The pump operates by energising a heater element immersed in the liquid nitrogen;...
Introduction to the Integra 750Plus Figure 1.2 Dewar and pump Although it is normal to use a Dura-Lo/Dura-Tech cylinder or Planer dewar, any liquid- nitrogen supply that has a source pressure between 5 and 22psi may be connected directly to the freezer.
Introduction to the Integra 750Plus Any program you run from the PC can be stored in the freezer's memory (overwriting any previously stored program) if the Mak e Permanent option is selected.. This enables you to rerun the program directly from the freezer, without having the PC connected.
If using a front-loading freezer, make sure that there will be sufficient room behind it for you to access the rear panel. Power sockets are required for the freezer, pump (if using a Planer dewar and pump), chart recorder and PC (if using DeltaT). Ensure that sufficient power sockets are nearby.
Installing the Integra 750Plus 3. Continue to lift the door until it is just beyond the vertical position, where it will remain. Do not force or slam the door back on its hinge or allow the door to fall back to the closed position, as personal injury or damage may occur.
2. Wear gloves and goggles, then open the liquid-nitrogen tap slowly whilst checking for leaks. Rectify as necessary. Installing a Planer Pump and Dewar Use the following procedures if your system uses a low-pressure Planer pump and dewar. If required, refer to Figure 1.2...
3. Fit the adaptor to the free end of the freezer's liquid-nitrogen delivery pipe and connect it to the Planer pump, making sure that the fibre sealing washer is undamaged and correctly positioned. Tighten the gland nut securely.
Installing the Integra 750Plus 3. Open the red pressure-release valve on the pump. 4. Slowly insert the pump into the neck of the dewar, being careful to avoid the liquid nitrogen splashing. It is normal for nitrogen gas to issue from the pressure-release valve immediately.
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Installing the Integra 750Plus To rotate a top-loading freezer so that the door is at the front 1. Place protective material on the bench where the front panel will be resting. 2. Each person should have one hand underneath the freezer at the rear, near the foot, and the other hand on the front panel at the front of the freezer.
Operating the Integra 750Plus Operating the Integra 750Plus This chapter describes how to operate and maintain the equipment on a day-to-day basis. Maintaining a Planer dewar Before you start a freezing program, you must ensure that there is sufficient liquid nitrogen to complete the program.
10. When the pump has attained ambient temperature, dry any condensation with absorbent tissue. If the pump is not dry when you put it back into the dewar (see Chapter 2: Installing the Integra 750Plus System ), the filter may become blocked with frozen water.
2. If you are using a Dura-Tech or Dura-Lo cylinder, make sure that the liquid-nitrogen supply tap is open. 3. If you are using a newly-filled Planer dewar and pump, switch on the pump power supply, then wait for the orange light to switch off; this indicates that the correct pressure has been attained.
However, if the equipment is to remain unused for longer than a working day, also turn off the liquid-nitrogen tap, or in the case of a Planer dewar, depressurise the dewar by opening the red pressure-release valve.
Operating the Integra 750Plus 2. If the solenoid valve has stopped for more than five minutes, estimate the temperature of the samples by: Estimating the amount of time that the program has been running for. Reading the temperature off from the DeltaT window.
The earth continuity of the mains installation must be regularly inspected by the person responsible for the safety of the installation. 3.4.2 Cleaning the Integra 750Plus freezer 1. Clean surface dirt with a cloth containing water and detergent. 2. Then clean all surfaces with 70% IPA (alcohol).
1. Disconnect the freezer from its mains wall socket. 2. If using a Dura-Tech or Dura-Lo cylinder, turn off the liquid-nitrogen tap. Otherwise, if using a Planer dewar, depressurise the dewar. 3. Open the door and pull the sample PRT out of its socket.
Spare Parts for details of fuse ratings, etc. If the freezer needs to be lifted and/or rotated to gain access, refer to the Lifting Instructions in Chapter 2: Installing the Integra 750Plus System 3.4.5 Disposal · Do not dispose of with general waste.
Troubleshooting Troubleshooting Integra 750Plus freezer Fan not running, RUN lamp fails to light when RUN pressed · Faulty mains supply. Remedy: check that the supply cable is properly connected and that the external fuse (if any) is intact. Also check the wall socket.
Troubleshooting Remedy: close door and turn handle fully clockwise. · If no manual cooling or heating - faulty door interlock switch. Remedy: refer to Service Engineer. No manual cooling after pressing STOP (RUN lamp off), but solenoid valve clicks. · No liquid-nitrogen supply due to low level, low pressure, supply valve off or blocked or leaking delivery hose.
Troubleshooting · Transformer fault. Remedy: refer to Service Engineer. · Relay fault. Remedy: refer to Service Engineer. Dewar Dewar loses pressure · Dewar overfilled Remedy: depressurise, check there is sufficient space at the top of the dewar, then repressurise as described in Chapter 2 ·...
Additional information Additional information Integra 750Plus specifications 5.1.1 Integra 750Plus freezer Note These specifications are subject to change without notice. Dimensions Front loading Top loading External Internal External Internal Height 55 cm 26 cm 48 cm 25 cm Width 79 cm...
Operating 5 ºC to +40 ºC. temperature Operating humidity Less than 90% Vibration Designed to survive normal shipment when packed in the Planer supplied container. Not designed to endure significant vibration when being operated. Sound pressure < 70 dB(A) level 5.1.2...
Ordering spares When ordering spares, always quote the model number, the serial number, the part number and the part description. Always use qualified parts from Planer Limited, except where parts are clearly marked with the original manufacturer's description and are...
Additional information 5.2.2 Integra 750Plus fuses For 230V AC operation Part number Description Ident. FL 010498 F 5A L 20x5mm, glass, F1, F2 250 V FL 011977 F 1A L 20x5mm, glass, 250 V FL 010496 F 2A L 20x5mm, glass,...
Index - A - - I - alarm Indications for use fitting information reviewing alarms installation - C - CryoCyl low pressure cylnder installing calibration pump and dewar manual configuring - K - system connecting delivery hose keys connection alarm - L - controller keys...
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Index - R - removing pump samples report system reset reviewing information print last print old starting - S - safety samples loading removing screen smaltimento specification controller system starting switching off symbols equipment system configuring installation print report specification switching off - U - unexpected...
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