Techne Barloworld Scientific HB-1D Operator's Manual

Hybridiser

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HB-1D

Hybridiser

Operator's Manual

Issue 25

Date of issue:

3rd May 2005

Techne is a trademark

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Summary of Contents for Techne Barloworld Scientific HB-1D

Page 3: Table Of Contents
Deutsch Español Italia INTRODUCTION Initial checks Specification Unpacking Installation Warning Servicing by Techne and their Agents Switching on Temperature control Speed control OPERATION The tubes The HB-1D The front panel controls Basic Protocol for Southern Hybridisations When the Protocol is Finished...
Page 4 H B - 1 D O P E R A T O R ’ S M A N U A L...
Page 5 H B - 1 D O P E R A T O R ’ S M A N U A L NOTES...
Page 6: English
Notwithstanding the description and specification(s) of the units Techne have taken great care in the design of these units to contained in the Operator’s Manual, Techne hereby reserves protect operators from hazards, but users should pay attention to...
Page 7: Français
L’appareil est garanti contre tout défaut ou vice de fabrication Techne a apporté un soin tout particulier à la conception de ces pour la durée figurant sur la carte de garantie, à compter de la appareils de façon à...
Page 8: Deutsch
Unfall zugrundeliegt. Der unter diese Garantie fallende Service und brennbare Materialien können sich leicht entzünden. wird vom Lieferanten geleistet. Techne hat bei der Konstruktion dieses Gerätes sehr darauf Ungeachtet der in dieser Gebrauchsanweisung enthaltenen geachtet, daß der Bediener vor Gefahren geschützt ist. Dennoch Beschreibungen und Spezifikationen, behält sich Techne hiermit...
Page 9: Español
Techne ha puesto gran cuidado en el diseño de estos aparatos A pesar de la descripción y las especificaciones de los aparatos para proteger al usuario de cualquier peligro; aún así se deberá...
Page 10: Italia
Questo Manuale è stato realizzato esclusivamente a vantaggio · Utilizzare con attenzione e indossare guanti protettivi; dei clienti della Techne e in alcun modo potrà essere utilizzato · Non mettere vicini oggetti caldi o oggetti infiammabili; come garanzia, condizione o rappresentazione concernente la ·...
Page 11: Introduction
Initial Checks When you receive your new HB-1D remove all the packing. Check the unit for any transit damage. If there is any transit damage at all inform Techne Sales Department or your Techne dealer immediately. When unpacking the unit, check that the following have been removed from the packing: a power cable;...
Page 12: Specification
H B - 1 D O P E R A T O R ’ S M A N U A L Specification Maximum Number of Hybridisation Tubes 6 (large and small) 24 mini tubes Temperature Range # ambient +10° to 100°C (NOTE: minimum temperature must be greater than 10°C above ambient) Rotation of Hybridisation Tubes 5 rpm to 20rpm and off...
Page 13: Unpacking
H B - 1 D O P E R A T O R ’ S M A N U A L Unpacking Ensure that the following have been removed from the packing: • The Hybridiser. • Drip Tray (not Techne Inc). • Power Lead. • Guarantee Card. • Decontamination Certificate.
Page 14 H B - 1 D O P E R A T O R ’ S M A N U A L SPEED CONTROL...
Page 15: Warning
11. Servicing by Techne and their Agents Please note that neither Techne nor any of their agents can accept the return of ANY GLASSWARE from these units. If the unit is returned it must be thoroughly decontaminated and a...
Page 16: Switching On
H B - 1 D O P E R A T O R ’ S M A N U A L Switching On Once your HB-1D is connected to a power supply, switch it on using the power switch next to the power inlet socket on the rear panel.
Page 17: Operation
Remove the tube end cap which has the holes in it. Place the filters in the tube and replace the end cap. A small quantity of Techne O-ring grease, see the list of accessories, must be smeared on the seal to facilitate the insertion of the end cap.
Page 18 H B - 1 D O P E R A T O R ’ S M A N U A L Small Tube (3 x 44mm dia) assembly Unscrew the tube end cap. Place the filters in the tube and replace the end cap.
Page 19: The Hb-1D
H B - 1 D O P E R A T O R ’ S M A N U A L The HB-1D Insert the tubes or tube holder into the HB-1D ensuring that they rotate freely and engage on the drive rollers. Close the door.
Page 20: The Front Panel Controls
H B - 1 D O P E R A T O R ’ S M A N U A L The Front Panel Controls The front panel controls consist of three buttons for controlling the display, a four digit LED display and three indicators. The SET temperature Button TEMPERATURE ∇...
Page 21 H B - 1 D O P E R A T O R ’ S M A N U A L Tube temperature NOTE: It takes approximately 50 minutes for the interior of the hybridisation tubes to reach the displayed temperature depending upon the number of filters and amount of hybridisation fluid used.
Page 22: Basic Protocol For Southern Hybridisations
H B - 1 D O P E R A T O R ’ S M A N U A L BASIC PROTOCOL FOR SOUTHERN HYBRIDISATIONS * INSERTION OF FILTERS Place a wet or moist filter, DNA side facing inwards, into a hybridisation tube and allow it to adhere to the glass.
Page 23: Technical Information
Techne Service department giving as much information as possible to help diagnose the fault. Please remember to return the unit in its original packing, Techne accept no responsibility for damage to units which are not properly packed: if in doubt, contact your supplier.
Page 24 You should contact your supplier and return the unit if necessary. Remember to fill in the Decontamination Certificate provided with the unit and return it to Techne Service department or your dealer giving as much information as possible to help diagnose the problem.
Page 25: Accessories And Spares
H B - 1 D O P E R A T O R ’ S M A N U A L Accessories and Spares The following may be purchased from Techne or their dealers. All Techne tubes are made from Borosilicate glass...
Page 26 H B - 1 D O P E R A T O R ’ S M A N U A L...
Page 27 Thank you for reading this data sheet. Please contact us for a quotation: Head Oﬃce (UK) Switzerland Oﬃce P.O. Box 2124 Zugerstrasse 46 Chelmsford 6314 Unterägeri Essex CM1 3UP England Switzerland Tel: +44 (0)1245 600 560 Tel: +41 41 541 5877 Fax: +44 (0)1245 600 030 Fax: +41 41 541 5878 Email:...