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Cairn FuraLED Quick Start

Dualport coupling
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FURALED
Q u i c k
S t a r t

DUALPORT COUPLING

The FuraLED dual port coupling houses two UV LEDs (typically 340nm and 385nm for Fura-2, although a
Fura-8 variant is available), photodiodes providing optical feedback for each channel, excitation filters and
a dichroic mirror to split the two wavelengths.
Each LED has individual focus (Z), and XY adjustments to optimise output when connected to your micro-
scope.
This short guide details how to align your FuraLED coupling. For general power supply operation, please
refer to the OptoLED user manual.
For the purpose of this Quick Start, all words in
bold blue
refer to parts labelled in diagrams throughout
this document.
340nm LED X&Y
adjustments
340nm LED
focus
385nm LED X&Y
adjustments
385nm LED
focus

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Summary of Contents for Cairn FuraLED

  • Page 1 S t a r t DUALPORT COUPLING The FuraLED dual port coupling houses two UV LEDs (typically 340nm and 385nm for Fura-2, although a Fura-8 variant is available), photodiodes providing optical feedback for each channel, excitation filters and a dichroic mirror to split the two wavelengths.
  • Page 2 Repeat the steps above for X, Y and Z adjustment for the 385nm LED Figure 1 Once the FuraLED channels have been aligned and focussed, the coupling should not need further ad- justment unless removed from the microscope. We recommend periodically checking the alignment to...
  • Page 3 Align the 340nm and 380nm channels on the FuraLED as per the previous instructions Perform any final positional adjustment (moving both UV LEDs equally) via the dichroic mirror FuraLED horizontal and vertical adjustments Adjust the on the dual port coupling.
  • Page 4 LED being fully pulled out of the coupling. Adjust the position of the transmitted LED using the transmitted LED X and Y adjustment thumb screws until centred. Please contact us if you have any questions or issues during setup or operation (tech@cairn-research.co.uk)

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