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Knauer BlueShadow Series Manual

Valves and valve drives
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BlueShadow
Valves and valve drives
Manual
V7695A
HPLC

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Summary of Contents for Knauer BlueShadow Series

  • Page 1 BlueShadow Valves and valve drives Manual V7695A HPLC...

  • Page 2: Table Of Contents

    Table of contents Table of contents Note: For your own safety, be sure to read the manual and always observe the warnings and safety information on the device and in the manual! Intended use ..........Overview valves and valve drives .
  • Page 3 Table of contents Mounting of the valve on the valve drive ......Screw the adapter plate to the back side of the valve....Screw the valve onto the valve drive .
  • Page 4 Table of contents Technical data ..........Ambient conditions .

  • Page 5: Intended Use

    Intended use Intended use High-pressure liquid chromatography (HPLC) is a method  HPLC for separating substance mixtures, determining substances,  and measuring their concentration. The device or system is suitable for high-pressure liquid chroma- tography. It is suitable for laboratory use, for analyzing sub- stance mixtures that can be dissolved in a solvent or solvent mixture.

  • Page 6: Multiposition Valves

    Intended use Multiposition valves Structure and function The multiposition valve is used in chromatography, for example in the following cases:  Selection of up to 13 different solvents  Fractioning  Column switching up to 16 columns Legend A. Position number B.

  • Page 7: Connections Of The Ports At The Positions Loading And Injecting

    Intended use Connections of the ports at the positions loading and injecting Valve position In the valve position loading, the sample is filled into the sample Loading (L) loop. Valve position In the valve position injecting, the sample is flushed for example Injecting (I) on a precolumn or main column for preseparation or separation.

  • Page 8: Series And Operating Modes

    Intended use Series and operating modes The following valves fit to the listed valve drives: Valve Switch posi- Operating mode tions 6V injection valve 6P/3C 6 ports/3 channels 6P/2C 6 ports/2 channels 6V Multiposition 7P/1C valve 7 ports/1 channel 12V Multiposition 13P/1C...

  • Page 9: Laboratory Use

    Intended use Room ventilation, Always use the device in rooms that are well-ventilated, and are A/C system, preferably equipped with an air-conditioning system. When set- sunlight ting up the system at the installation location, make sure that it is protected against direct sunlight. ...

  • Page 10: Selection Of A Column

    Intended use Selection of a column Basics The selection of a column, also called column selection, is used in chromatography, for example in the following cases:  Method development  Column selection Injection From the injection valve, the sample is injected via the channel 2->1 onto the column A.

  • Page 11: Column Selection With A 2-Channel Valve

    Intended use Column selection with a 2-channel valve  Separation of the sample on the precolumn  Separation of the sample components on different columns The sample is separated on the precolumn. The further separa- tion can be carried out on column 1 and be continued on col- umn 2 after switching over.

  • Page 12: Backflushing Of A Column

    Intended use Backflushing of a column Basics The backflushing of a column is used in chromatography, for example in the following cases:  Analysis of heavily retarding substances  Optimization of analysis times  Flushing of a column Note: The eluent flushes the column after switchover in opposite direction (reduction of the band broadening).

  • Page 13: Backflushing Of A Precolumn

    Intended use Backflushing of a precolumn Basics The precolumn is used in chromatography, for example in the following cases:  Preseparation or separation of heavily retarding substances  Protection of the main column Note: Precolumn and main column are aligned in a row behind each other.

  • Page 14: Accumulation Of A Sample

    Intended use Accumulation of a sample Basics The accumulation of a sample is employed in chromatography, for example in the following case:  Samples that are very diluted by solvents Note: The solvent is fed through a detector A into the waste container.

  • Page 15: Precleaning Of A Sample By A Precolumn

    Intended use Precleaning of a sample by a precolumn Basics The precleaning of a sample is used in chromatography, for example in the following cases:  Preseparation or separation of heavily retarding substances  Precleaning samples, alternating and time-delayed on differ- ent precolumns Injection on precolumn...

  • Page 16: Precleaning Of A Sample

    Intended use Precleaning of a sample Basics The precleaning of a sample is used in chromatography, for example in the following cases:  Preseparation or separation of heavily retarding substances  Precleaning samples, alternating and time-delayed on differ- ent precolumns ...

  • Page 17: Alternating Precleaning Of A Sample

    Intended use Alternating precleaning of a sample Basics The precleaning of a sample is used in chromatography, for example in the following cases:  Preseparation or separation of heavily retarding substances  Precleaning samples, alternating and time-delayed on differ- ent precolumns ...

  • Page 18: Fig. 18: Alternating Sample Precleaning - Precolumn B Is Purged And

    Intended use Note: Make sure there is no pressure when switching between the columns so that the columns are not damaged. Switchover of the direction of flow Pump A Injection Eluent A Detector A Pump B Eluent B Waste Detector B Fig.

  • Page 19: Symbols And Labels

    Symbols and labels Symbols and labels Explanations of symbols and labels on the device or system Symbol Explanation Hazard symbol indicating microelec- tronic devices that can be damaged by electrostatic discharge when touched. Electrostatic Discharge Setting of descending values < Setting of increasing values >...

  • Page 20: Safety

    Safety Symbol Explanation For your own safety, be sure to read the manual and always observe the warnings and safety information on the device and in the manual! Safety Laboratory regulations  Adherence Observe national and international regulations pertaining to laboratory to laboratory work! regulations ...

  • Page 21: Peek Connections

    Safety Note: Even small quantities of other substances, such as addi- tives, modifiers, or salts can influence the durability of the materials. The list of selected solvents was com- piled based on research in the pertinent literature and is only a recommendation by the manufacturer. If there is any doubt, contact the technical customer ser- vice department of the manufacturer.

  • Page 22: Power Supply And Mains Connection

    Safety Power supply and mains connection The device is intended for operation with direct current of 24 volt and 1.5 amperes. The supplied power supply is to be used to connect the device to the mains. Target group To what should the user pay particular attention? To make your HPLC separations as efficient as possible, pay close attention to the following:...

  • Page 23: Installation

    2. If a part is missing, inform the technical service department of the manufacturer. Technical customer services of the manufacturer: Technical Languages: German and English customer services Available by telephone: 8 am to 5 pm (CET) Europe Telephone: +49-(0)30-809727-0 Fax: +49-(0)30-8015010 E-mail contact: E-mail: info@knauer.net (manufacturer)

  • Page 24: Space Requirements

    Installation Space requirements  Side clearance to other devices:  If there is a device on one side, min. clearance of 5 cm.  If there are devices on both sides, min. clearance of 10 cm. Note: Make sure that the power plug on the rear of the device is always accessible, so that the device can be disconnected from the power supply.

  • Page 25: Rear View Of The Device

    Installation Rear view of the device The rear of the device contains the mains connection socket, ground for grounding the device, connections for external devices, symbols, warning signs and serial number. Legend A. Serial number B. CE mark C. Warning of the sup- ply voltage D.

  • Page 26: Mounting Of The Valve On The Valve Drive

    Installation Legend A. Axial plate B. Axial needle bearing C. Compensation plate D. Disk spring E. Rotor F. Rotor seal G. Seal ring Fig. 21 Exploded view of an electrical valve Mounting of the valve on the valve drive Delivery ex works All valve drives are delivered with a ready-mounted injection or multiposition valve.

  • Page 27: Screw The Valve Onto The Valve Drive

    Installation Screw the valve onto the valve drive Caution! Risk of mix-up! Always mount the switch or injection valve onto the matching valve drive. An operation of the valve with the wrong valve drive can block the eluent flow. Damage to the device or the system might be the consequence.

  • Page 28: Mounting Plate For Valves And Column Holder

    Installation Mounting plate for valves and column holder Valves can be attached on the side of housings of pumps, detec- tors, valves, or multifunction modules (assistant) with a mount- ing plate (order number G6725) and 4 screws. Legend A. Valve drive B.

  • Page 29: Inserting The Needle Seal In The Syringe Connection

    Installation Prerequisite The capillary end was cut off cleanly and at a right angle to the capillary axis with a tube cutter (order number A0569). Connecting the capillar- Steps Figure 1. Place the biconical seal (C) on the nee- dle seal (B). 2.

  • Page 30: Multifunction Module 'Assistant

    Installation The injection valve is prepared for the injection of sample solu- tions in the sample loop via a glass syringe with luer lock: Legend A. Injection valve B. Syringe connection C. Needle seal D. Sample loop E. Metal cannula with luer lock F.

  • Page 31: Connection Of The Pump With Other Devices

    Installation Connection of the pump with other devices Control of the pump with chromatography software The valves can be controlled individually or as part of a HPLC system by means of a computer and chromatography software. Local area network and automatic configuration The valve is controlled either by means of the function keys on the front of the device or by means of the chromatography soft-...

  • Page 32: Remote Terminal Strip

    Installation The connections at the interfaces are detected automatically by the device. The LAN connection and the RS-232 interface can be used for the valves. The remote control is often used when the devices are employed in an analytical system whose chromatography software does not support the driver software of the manufac- turer.
  • Page 33 Installation HOME If the valve is controlled by HOME , it has priority over control via RS-232 or manual operation. As long as the switch-off signal is not cancelled, the valve cannot be started. The indicator of the position flashes to indicate that the start of the valve is pre- vented by an external signal.
  • Page 34 Installation Binary code Prerequisite: The DIP switch of the device was set for the binary control. A binary code is entered during binary control so that the valve can be set externally in the correct position (nominal position). Position BIN 0 BIN 1...

  • Page 35: Operating The Valves And Valve Drives

    Operating the valves and valve drives Operating the valves and valve drives The device can be operated by means of the chromatography software at the workstation or the keyboard on the device. Operator errors and clogged capillaries can cause high pressure spikes.

  • Page 36: The Keyboard For Operating The Device

    Operating the valves and valve drives  Prerequisites Preparation of the analog mode via DIP switch on the device bottom (DIP switch 2 is set to OUT position)  Connect a cable from the analog output of the valve, for example to a pump. ...

  • Page 37: Set The Position Manually On The Valve

    Operating the valves and valve drives Set the position manually on the valve.  Press one of the two buttons to adjust the value of the cur- rent injection position. The [<] button reduces the value of the injection position; the [>] button increases it. Practical tip! If several valves are switched in series, then the injection posi- tions are synchronized simultaneously on all valves.

  • Page 38: Injecting The Sample

    Operating the valves and valve drives Injecting the sample  Prerequisites The data acquisition was started. 1. Set the valve to I (= inject). The sample is injected and mea- sured. In the Display, an I is displayed for injecting (A). Fig.

  • Page 39: Binary Control

    Operating the valves and valve drives Command lines for terminal program Command Action Comment 1, 2, 3, … 6 Valve sets the entered Only 360° position valves 1, 2, … 15, 16, Valve sets the entered Only 360° 01, … position valves H, h...

  • Page 40: Connect Valve Drive To The Devices Manager Or Interface Box

    Operating the valves and valve drives Connect flat ribbon cable Steps Figure to the connector strip 1. Place the connector strip (C) on a suitable surface. 2. Put the stylus (A) in the opening on the top of the connector strip and press downward.

  • Page 41: Fig. 38 Basic Functions Of The Dip Switches

    Operating the valves and valve drives DIP configuration of the electric valve drive The DIP switches for configuration of the electric valve drive are set and checked on the device bottom.  Prerequisite Device is switched off.  Switch on the device after the new configuration so that the new configuration becomes effective.

  • Page 42: Fig. 40: Combination Of Valve Drive, Valve, Operating Mode, And Setting

    Operating the valves and valve drives Drive Valve Mode DIP1 DIP2 DIP3 DIP4 6P/2C Analog 6P/3C Analog 7P/1C Analog 7P/1C Binary 13P/1C Analog 13P/1C Binary 17P/1C Analog 17P/1C Binary Fig. 40 Combination of valve drive, valve, operating mode, and setting of the DIP switches.

  • Page 43: Maintenance And Care

    Maintenance and care Maintenance and care Removing the valve Caution! During assembly, make sure the rotor seal is in the right position and installed properly, because other- wise there may be a mix-up of the channels or clog- ging may occur. Note: During disassembly, make sure to mark the position of the rotor seal on the cylinder pins to make the assem-...

  • Page 44: Fig. 44 Inner Components Of The Valve

    Maintenance and care Steps Figure 4. Hold the inner com- ponents of the valve and turn over the bottom part in order to deposit the inner parts orderly. Rotor plate with micro cylinder pins (A), four disk springs in two groups of two (B), rotor seal (C), several sealing disks (D) inside the...

  • Page 45: Alignment Of The Rotor Seal In The Valve

    Maintenance and care Alignment of the rotor seal in the valve Valve type Position of the rotor seal during installation 1-channel cylindrical pin markings rotor rotor with sealing 2-channel 3-channel...

  • Page 46: Insert New Needle Seal

    Maintenance and care Insert new needle seal. Steps Figure 1. Removing the old needle seal 2. Insert the new nee- dle seal (B) into the screw fitting (A). 3. Place the biconical seal (C) on the new needle seal and turn everything into the syringe connection at the valve.

  • Page 47: Cleaning And Caring For The Device

    Environmental protection Cleaning and caring for the device Note: Risk of damage to the device if cleaning solution enters the device's interior! Only lighty moisten the cleaning cloth! All smooth surfaces of the device can be cleaned with a mild, commercially available cleaning solution, or with isopropanol.

  • Page 48: Troubleshooting

    Troubleshooting Troubleshooting The display shows the status of the error: Display Cause of the fault Solution Setting the position Replacing the rotor of the valve was not seals of the valve changed The value of the motor Replacing the rotor current is too high seals of the valve The change from one...

  • Page 49: Technical Data

    Technical data Technical data Ambient conditions Temperature range 4–40 ˚C; 39.2–104 ˚F below 90 % humidity  Air humidity (non-condensing) Valve drive  Control  RS-232  Terminal strip: Remote  Buttons on the device  HPLC software EZChrom Elite as of version 3.3.2 plus driver of the manufacturer ...

  • Page 50: Valve

    Technical data Valve Electrical valves  Material Stainless steel; 1/16¨; 1/8¨  Peek; 1/16¨; 1/8¨  Screw fitting UNF 10/32; 1/16¨  DYNASEAL; 1/16¨; 1/8¨ Switch duration of the approx. 300 ms; via reed contact valve  Seals Rotor seals made of VESPEL, TEFZEL, POM-H-TF or ETFE ...

  • Page 51: Abbreviations And Terminology

    Abbreviations and terminology Abbreviations and terminology Here you can find information on the abbreviations and termi- nology used in this manual on liquid chromatography. Terminology Explanations Backflushing Backflushing of columns or precolumns to separate heavily retarding substances by changing the flow direction. Microelectronic devices can be damaged by electrostatic discharge when touched.

  • Page 52: Legal Information

    4. Damage caused by negligence or improper operation of the device and damage caused by clogged capillaries 5. Packaging and transport damage In the event of device malfunctions, contact: Manufacturer Wissenschaftliche Gerätebau Dr. Ing. Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin, Germany Telephone: +49-(0)30-809727-0 Fax: +49-(0)30-8015010...

  • Page 53: Declaration Of Conformity

    Declaration of conformity Declaration of conformity Manufacturer name Wissenschaftliche Gerätebau and address Dr. Ing. Herbert KNAUER GmbH Hegauer Weg 38 14163 Berlin, Germany Device Order number Valve drive and valve C55000.0; C55020.0 in a multifunction C55100.0; C55110:0 module (‘Assistant’) C55200.0; C55210.0 complies with the following requirements and product...

  • Page 54: Table Of Figures

    Table of Figures Table of Figures Fig. 1: Overview valves and valve drives ......6 Fig.
  • Page 55 Table of Figures Fig. 38: Basic functions of the DIP switches ......42 Fig. 39: Details of the setting of the DIP switches among the different series of the valve drives .

  • Page 56: Index

    Index Index Switch Abbreviations DIP configuration AC system Disconnecting from power supply Accessories Disk springs Accumulation of a sample Pressure stability Adapter plate Display Ambient conditions Disposal ambient conditions Drived, see Valve drives Application examples Dynaseal Assistant, see Multifunction module Electrical connections Backflushing Environmental protection...
  • Page 57 Index Loading a sample Samples Injecting a sample Accumulation Local network Backflushing Luer lock Filling in sample loop Precleaning Scope of delivery Mains connection Seals Maintenance Dynaseal Manufacturer see technical customer services Markings Selecting a column Mounting plate Selection of a solvent Mounting the valve Self-ignition temperature Multi-channel valves, see injection valves...
  • Page 58 Index Valve drives Operating externally Warnings Operating manually Warranty conditions Operation Valve positions Loading and injecting Valves Adapter plate Chromatography software Electrical Exploded view Increasing the pressure stability Manual Manual setting Operation Setting the position...
  • Page 59 © Wissenschaftliche Gerätebau Dr. Ing. Herbert Knauer GmbH All rights reserved. The information in this document is subject to change without prior notice. Translation of the original German edition of this manual. 2011-01-27 Printed in Germany. www.knauer.net HPLC · SMB · Osmometry Wissenschaftliche Gerätebau...

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